##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939809_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 789281 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.750409549957492 31.0 30.0 33.0 16.0 34.0 2 29.560009679695824 31.0 30.0 33.0 25.0 34.0 3 29.763294694791842 31.0 30.0 33.0 25.0 34.0 4 33.5863095652879 35.0 33.0 37.0 28.0 37.0 5 33.62124769252016 35.0 35.0 37.0 28.0 37.0 6 33.73631951104866 35.0 35.0 37.0 28.0 37.0 7 33.68136569865485 35.0 35.0 37.0 28.0 37.0 8 33.57537429635326 35.0 33.0 37.0 28.0 37.0 9 34.72617356809552 37.0 34.0 39.0 27.0 39.0 10-11 34.60238748937324 37.0 34.0 39.0 27.0 39.0 12-13 34.50516419374088 37.0 34.0 39.0 27.0 39.0 14-15 34.91231639935587 38.0 33.0 40.0 25.5 41.0 16-17 34.85242644888196 38.0 33.0 40.0 25.5 41.0 18-19 34.97751687928634 38.0 33.5 40.0 26.5 41.0 20-21 35.25214023902767 38.0 34.0 40.0 27.0 41.0 22-23 35.12392164514286 38.0 34.0 40.0 26.0 41.0 24-25 34.861026681245335 38.0 34.0 40.0 25.0 41.0 26-27 34.600455351136034 38.0 33.0 40.0 24.5 41.0 28-29 34.47083864935301 38.0 33.0 40.0 24.0 41.0 30-31 34.04491302843981 37.5 33.0 40.0 23.5 41.0 32-33 33.46102275362007 37.0 33.0 40.0 20.0 41.0 34-35 33.135002616305215 36.0 32.0 40.0 18.5 41.0 36-37 32.742188776874144 36.0 31.5 40.0 16.0 41.0 38-39 32.34378250585026 36.0 31.0 39.5 14.5 41.0 40-41 31.915836691875263 35.0 31.0 39.0 9.0 41.0 42-43 31.262650437550125 35.0 30.0 39.0 5.0 41.0 44-45 30.596715871787108 35.0 29.0 39.0 2.0 40.5 46-47 29.84284240973747 35.0 26.5 39.0 2.0 40.0 48-49 29.236700237304586 35.0 25.0 39.0 2.0 40.0 50-51 28.50986340732895 34.5 24.0 38.5 2.0 40.0 52-53 28.559691035258673 35.0 24.0 38.0 2.0 40.0 54-55 28.591108236483585 35.0 24.0 39.0 2.0 40.0 56-57 28.266783312913905 35.0 23.0 38.5 2.0 40.0 58-59 27.85824566915965 34.0 22.5 38.0 2.0 40.0 60-61 27.409471404987578 34.0 21.5 38.0 2.0 40.0 62-63 26.894014299089932 34.0 19.0 38.0 2.0 40.0 64-65 26.284521608907347 33.0 15.0 37.5 2.0 40.0 66-67 25.653810239952563 33.0 8.0 36.5 2.0 39.0 68-69 25.072422876009938 32.0 6.5 36.0 2.0 39.0 70-71 24.448378334205437 31.0 2.0 35.5 2.0 38.5 72-73 23.730620653480827 31.0 2.0 35.0 2.0 38.0 74-75 23.33738047666167 31.0 2.0 35.0 2.0 37.5 76-77 22.71607767069016 30.0 2.0 35.0 2.0 37.0 78-79 22.00915706826846 29.5 2.0 34.0 2.0 36.0 80-81 21.261997311477153 29.0 2.0 34.0 2.0 36.0 82-83 20.50980069202223 28.0 2.0 34.0 2.0 35.0 84-85 19.79503370789364 26.5 2.0 34.0 2.0 35.0 86-87 19.030181899728994 25.5 2.0 33.0 2.0 35.0 88-89 18.157905106039546 24.5 2.0 33.0 2.0 35.0 90-91 16.95074377819813 17.5 2.0 32.0 2.0 34.5 92-93 15.678247670981563 7.5 2.0 31.0 2.0 34.0 94-95 15.080590436105773 2.0 2.0 31.0 2.0 34.0 96-97 14.599546929420574 2.0 2.0 31.0 2.0 34.0 98-99 13.771321620563526 2.0 2.0 31.0 2.0 34.0 100-101 12.339479222228839 2.0 2.0 28.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.9413144622648169 1101 2 1.2997387268934695 1101 3 0.8867001976124804 1101 4 0.7345932820791035 1101 5 0.8467879872028377 1101 6 1.0795075711992297 1101 7 0.8342628707809041 1101 8 1.0486060264942196 1101 9 1.0053416790325684 1101 10-11 1.0689078942885715 1101 12-13 1.218984564104467 1101 14-15 1.0971679818041764 1101 16-17 1.1468594769384879 1101 18-19 0.9220384406857676 1101 20-21 0.9060579093651384 1101 22-23 1.386773741621191 1101 24-25 1.320612847863302 1101 26-27 0.94280183655043 1101 28-29 1.4631254268555338 1101 30-31 1.2931646915691317 1101 32-33 1.3812166773427705 1101 34-35 1.6512917995533556 1101 36-37 1.4182383043511564 1101 38-39 1.3892612892580942 1101 40-41 1.5841137659931448 1101 42-43 2.0469041706801967 1101 44-45 2.286263124584732 1101 46-47 1.9517895556585962 1101 48-49 1.87301931559929 1101 50-51 1.901416604993269 1101 52-53 1.3708157062031177 1101 54-55 1.7845390029641912 1101 56-57 2.1429123485093875 1101 58-59 2.0595940055064226 1101 60-61 1.5793786384950756 1101 62-63 1.6374994704399448 1101 64-65 1.7684306924043902 1101 66-67 1.6544728820660737 1101 68-69 1.9920568452454717 1101 70-71 2.0398886494636876 1101 72-73 2.43044132783146 1101 74-75 2.0810439144270845 1101 76-77 1.8544685745400677 1101 78-79 1.4837682250857824 1101 80-81 0.9872686969679378 1101 82-83 0.44436929077215126 1101 84-85 0.07270330999326902 1101 86-87 -0.4387078082650149 1101 88-89 -3.9850818839435256 1101 90-91 -6.727736924785987 1101 92-93 -8.655252215238527 1101 94-95 -6.477030058981002 1101 96-97 -3.508145754355475 1101 98-99 -2.5164171727554088 1101 100-101 -1.824016130059249 1102 1 1.7078038758661407 1102 2 1.0710944689170816 1102 3 0.6248087935730631 1102 4 1.0361714848305894 1102 5 0.6542082233234296 1102 6 0.9256998826442455 1102 7 0.8085408491202557 1102 8 0.9226913031065749 1102 9 1.192823384564818 1102 10-11 1.4436456735841148 1102 12-13 1.1819687576843236 1102 14-15 1.370164127769634 1102 16-17 1.442924946740419 1102 18-19 1.557905354531016 1102 20-21 1.3918309116092615 1102 22-23 1.303710759866874 1102 24-25 1.4196468999659473 1102 26-27 1.3881867521519524 1102 28-29 1.3803795492089392 1102 30-31 1.4192684577902241 1102 32-33 1.5060947142244103 1102 34-35 1.3791920825077923 1102 36-37 1.4574982321489856 1102 38-39 1.503577320090372 1102 40-41 1.277504584609595 1102 42-43 1.5777303488439252 1102 44-45 1.913135258451451 1102 46-47 1.4708158021216668 1102 48-49 1.7159857025638736 1102 50-51 2.055504808720464 1102 52-53 1.8574729410552955 1102 54-55 1.8840548093843275 1102 56-57 1.9303916147792641 1102 58-59 1.9410519526232157 1102 60-61 1.8760722183565264 1102 62-63 1.9946684625367332 1102 64-65 1.9205430936142633 1102 66-67 1.8515286923890315 1102 68-69 1.9732623287072713 1102 70-71 1.718717071748788 1102 72-73 1.9063353174889848 1102 74-75 1.459416194649549 1102 76-77 1.3741107347508184 1102 78-79 1.2811265039422537 1102 80-81 1.3841122914649624 1102 82-83 1.3135699444944677 1102 84-85 1.0204543100908374 1102 86-87 1.127109588554184 1102 88-89 0.41976858927337446 1102 90-91 -4.442770098754178 1102 92-93 -8.189304317882687 1102 94-95 -6.464730078495101 1102 96-97 -3.212031499306203 1102 98-99 -1.8447784762972184 1102 100-101 -1.2314217306056463 1103 1 1.840243992256699 1103 2 0.9152511792996876 1103 3 0.9159352640877323 1103 4 0.6513197660688661 1103 5 0.8217739431356748 1103 6 0.6397176080258973 1103 7 0.6503714158155489 1103 8 0.672568325976151 1103 9 0.6808925185556944 1103 10-11 0.9031215825727017 1103 12-13 0.7231431684362875 1103 14-15 0.8534058883019 1103 16-17 0.6338062967374967 1103 18-19 0.8977265999766928 1103 20-21 0.7977742504175112 1103 22-23 0.8789831854761303 1103 24-25 0.9875031793032889 1103 26-27 0.965147507856841 1103 28-29 0.8015991488454262 1103 30-31 0.6714299371842642 1103 32-33 0.8491884019542155 1103 34-35 0.6543502852908247 1103 36-37 0.5986655560832546 1103 38-39 0.561933095013039 1103 40-41 0.3617376658745499 1103 42-43 0.32327190677657214 1103 44-45 0.04976493470894283 1103 46-47 0.18087950010651 1103 48-49 0.3331224614703352 1103 50-51 0.2565286454401843 1103 52-53 0.1062410853929272 1103 54-55 0.07844621849380573 1103 56-57 0.17241269180880536 1103 58-59 0.12724349679909963 1103 60-61 0.07011345529802426 1103 62-63 0.11027738692456168 1103 64-65 0.10728282398169497 1103 66-67 8.541940940389736E-4 1103 68-69 -0.027362978735553156 1103 70-71 -0.4532570655353716 1103 72-73 -0.1679178569884634 1103 74-75 -0.11961849740738018 1103 76-77 -0.04937247782870102 1103 78-79 -0.45854967017668713 1103 80-81 -0.8155838091178254 1103 82-83 -1.246228206262991 1103 84-85 -1.2521359634603257 1103 86-87 -1.2330987638857636 1103 88-89 -1.8110719906784354 1103 90-91 -6.069624135322158 1103 92-93 -8.315791507417542 1103 94-95 -6.778661511449638 1103 96-97 -4.431020418858315 1103 98-99 -3.4111256803392056 1103 100-101 -2.8031840971025055 1104 1 2.1079034650607156 1104 2 1.1804392550077907 1104 3 1.0719518285537681 1104 4 0.925498842812253 1104 5 0.8997702798402969 1104 6 0.8168405938770817 1104 7 0.8920214914705582 1104 8 0.8628340476187901 1104 9 1.0876032509493143 1104 10-11 1.0738046013404556 1104 12-13 0.8334999415508761 1104 14-15 0.9404927721117247 1104 16-17 1.041244379082002 1104 18-19 1.029929089955722 1104 20-21 0.9508893787655737 1104 22-23 0.896625403628029 1104 24-25 1.1068974082857963 1104 26-27 1.069098823192249 1104 28-29 1.0605198039912906 1104 30-31 1.3803851016343742 1104 32-33 1.1427497621517162 1104 34-35 1.2410867669345294 1104 36-37 1.1070314077994325 1104 38-39 0.873984805878159 1104 40-41 0.9698818616750273 1104 42-43 1.1661842265981868 1104 44-45 1.0820032612731474 1104 46-47 1.002053612819207 1104 48-49 0.8582903625082672 1104 50-51 0.7951724297835803 1104 52-53 0.8673052992444319 1104 54-55 1.1704546600005372 1104 56-57 1.066467800513113 1104 58-59 0.9751384292405021 1104 60-61 0.7670261998495356 1104 62-63 1.0362801476689008 1104 64-65 0.9452868589157291 1104 66-67 1.0059562619977314 1104 68-69 0.9993339499363714 1104 70-71 0.8489714391499277 1104 72-73 0.776981402388774 1104 74-75 0.4672546941723468 1104 76-77 0.6469934269787139 1104 78-79 0.6211323133119357 1104 80-81 0.7485610237775084 1104 82-83 0.17545425504677326 1104 84-85 0.10600557777829422 1104 86-87 0.4123911355063399 1104 88-89 0.4405433102161531 1104 90-91 -1.9502206153731247 1104 92-93 -6.5082034423276856 1104 94-95 -3.889926144721917 1104 96-97 -2.0689487987792106 1104 98-99 -1.3809791485242808 1104 100-101 -0.7954513583910305 1105 1 2.023816867582756 1105 2 1.4520718852945897 1105 3 1.2694409480716793 1105 4 1.126692905974842 1105 5 0.9186632605344158 1105 6 1.0296628235389491 1105 7 1.3031140034669875 1105 8 0.9461394822801026 1105 9 1.078988141028546 1105 10-11 1.0897849606753454 1105 12-13 0.9923270813790239 1105 14-15 1.245854896874583 1105 16-17 1.0655050642733954 1105 18-19 1.1315416331986654 1105 20-21 1.1181140188727028 1105 22-23 1.0494989668464143 1105 24-25 1.1499227402799974 1105 26-27 0.592762476802335 1105 28-29 0.8393335524568641 1105 30-31 1.4302623474774663 1105 32-33 1.6449816559135755 1105 34-35 1.5199395735409311 1105 36-37 1.6896784338009994 1105 38-39 1.4089083635168222 1105 40-41 0.9890873074958151 1105 42-43 0.9054878757444875 1105 44-45 0.8128145546555778 1105 46-47 1.1652943225614223 1105 48-49 1.080603720392432 1105 50-51 1.5017620961154599 1105 52-53 1.5709600252794758 1105 54-55 1.704764446854682 1105 56-57 1.4671820065169072 1105 58-59 1.1208476134833312 1105 60-61 0.9884412204945541 1105 62-63 1.1077063277827257 1105 64-65 1.1939533023930196 1105 66-67 1.1518160990694852 1105 68-69 1.0389277452717103 1105 70-71 0.9784491760096508 1105 72-73 1.1494509928362682 1105 74-75 0.8626793119605409 1105 76-77 0.8187878695632484 1105 78-79 0.4086895055895887 1105 80-81 0.21439631001788229 1105 82-83 -0.16216989404373194 1105 84-85 -0.13426336542000783 1105 86-87 0.2576372017935853 1105 88-89 -0.09283466099241977 1105 90-91 -2.601381200129291 1105 92-93 -6.788769227396315 1105 94-95 -4.850483002215499 1105 96-97 -2.812320074306495 1105 98-99 -1.7446271788780372 1105 100-101 -1.1423490260620497 1106 1 1.5859598913930029 1106 2 0.9094883668155234 1106 3 0.5478138975192834 1106 4 0.41693039976104984 1106 5 0.6982028817211798 1106 6 0.587164522821702 1106 7 0.7359086388059808 1106 8 -0.2557657433947895 1106 9 -0.2592097062084733 1106 10-11 -0.7233218354411548 1106 12-13 -0.4494154189506858 1106 14-15 0.2560100134910428 1106 16-17 0.5407741596462756 1106 18-19 0.7614860387540574 1106 20-21 0.6841704654949368 1106 22-23 0.7843474516948987 1106 24-25 0.7253404594874624 1106 26-27 0.5812200353134571 1106 28-29 0.6607287904071768 1106 30-31 0.868002656493303 1106 32-33 0.7838155928727204 1106 34-35 0.8412435067464443 1106 36-37 0.8520157338182415 1106 38-39 0.9237758194622856 1106 40-41 0.843131600442014 1106 42-43 1.363989869231414 1106 44-45 1.301021025118498 1106 46-47 1.0836628082215043 1106 48-49 1.3149779278165248 1106 50-51 1.371191238561007 1106 52-53 1.279948977405354 1106 54-55 1.410423312038155 1106 56-57 1.3750884901682845 1106 58-59 1.2618677918319356 1106 60-61 1.0240272447816139 1106 62-63 1.1643888206051578 1106 64-65 1.3523923970557519 1106 66-67 1.2320273173791279 1106 68-69 1.3176202613261268 1106 70-71 0.9299536753045778 1106 72-73 0.9667933146144811 1106 74-75 0.7948931476728696 1106 76-77 1.3083309400403635 1106 78-79 1.2429713937717075 1106 80-81 1.2722809819802237 1106 82-83 1.3082651223403943 1106 84-85 1.1001539581335713 1106 86-87 1.379214541984922 1106 88-89 0.5915882422955683 1106 90-91 -2.5143271984452262 1106 92-93 -6.701045657583693 1106 94-95 -4.469706708985239 1106 96-97 -2.1992518290822396 1106 98-99 -1.4275308726622118 1106 100-101 -1.1542287878581146 1107 1 1.7152331607349005 1107 2 0.8787310552740522 1107 3 0.7210714775384091 1107 4 0.9753178973958896 1107 5 0.5845089871587419 1107 6 0.5587468022736957 1107 7 0.4702742900304955 1107 8 0.6430463069051342 1107 9 0.5597264063373615 1107 10-11 0.675536239599623 1107 12-13 0.7128946650700314 1107 14-15 0.9915812572329514 1107 16-17 0.4591748510401601 1107 18-19 0.4890438637270762 1107 20-21 0.5758448455672465 1107 22-23 0.37929777054408476 1107 24-25 0.4794021428679329 1107 26-27 0.4931964399629791 1107 28-29 0.28445991387110325 1107 30-31 0.8143250240059743 1107 32-33 1.1720713574715163 1107 34-35 0.8884175876579334 1107 36-37 0.6174634267002617 1107 38-39 0.3483514969208983 1107 40-41 0.4932144449755569 1107 42-43 0.24214192474747875 1107 44-45 0.5340724142985884 1107 46-47 0.512390940077271 1107 48-49 0.44784240541014597 1107 50-51 0.443974252363315 1107 52-53 0.6631969945061016 1107 54-55 0.6981533718191777 1107 56-57 0.5778082966598213 1107 58-59 0.6363570450549432 1107 60-61 0.5731867818749983 1107 62-63 0.1135532158656325 1107 64-65 0.3399222492140552 1107 66-67 0.6880417282368754 1107 68-69 0.7784033467224774 1107 70-71 0.9162306317396052 1107 72-73 0.644979377300178 1107 74-75 0.6892484688999936 1107 76-77 0.5150242158257221 1107 78-79 0.37472872065475826 1107 80-81 0.18730396878135025 1107 82-83 -0.06373495948136565 1107 84-85 0.09328220863089243 1107 86-87 0.5004731140929053 1107 88-89 0.16647944492204303 1107 90-91 -2.6077190684304465 1107 92-93 -3.6378588022496814 1107 94-95 -1.8602445336514304 1107 96-97 -0.7497742098909459 1107 98-99 -0.3772974249488765 1107 100-101 0.16498347313284967 1108 1 1.884112288023534 1108 2 1.1760598804492588 1108 3 -0.5563278863373888 1108 4 0.02269912535254548 1108 5 0.6122341596322443 1108 6 0.7382687066363403 1108 7 0.8338438763674958 1108 8 0.8763125039213691 1108 9 1.1561192722682634 1108 10-11 0.946510566793485 1108 12-13 1.0256016560567573 1108 14-15 0.8622612174979665 1108 16-17 0.850751217841399 1108 18-19 1.1364050419238723 1108 20-21 0.8997560069040205 1108 22-23 0.7161656335130857 1108 24-25 0.6272563628867047 1108 26-27 0.8554245150948958 1108 28-29 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96-97 2.132525865817751 2311 98-99 1.5271758575739565 2311 100-101 1.6836169049049747 2312 1 1.0905879292802183 2312 2 0.5501092587156151 2312 3 -0.24239744842065036 2312 4 -0.8211826551484478 2312 5 -0.10542067307034131 2312 6 -0.19732329192195408 2312 7 0.2716284621787537 2312 8 0.4064380520681823 2312 9 -0.020813508412942383 2312 10-11 -0.02493473866696405 2312 12-13 0.17869831010853687 2312 14-15 0.26442677285329097 2312 16-17 -0.19142154601995998 2312 18-19 -0.6530705392619254 2312 20-21 0.033647999455233446 2312 22-23 0.18713337544856756 2312 24-25 0.11112432708446107 2312 26-27 0.2527843997588022 2312 28-29 0.1716803527491706 2312 30-31 -0.10528416050874512 2312 32-33 -0.22729102390686506 2312 34-35 -0.14632517225717123 2312 36-37 -0.12987997521873496 2312 38-39 -0.14208860902729015 2312 40-41 -0.1674373819289734 2312 42-43 -0.3914168115923964 2312 44-45 -0.583829145104314 2312 46-47 -0.4672456085378549 2312 48-49 -0.019203148800595926 2312 50-51 -0.18979234589309968 2312 52-53 -0.34589455244262624 2312 54-55 -0.3099865709968661 2312 56-57 -0.2746334230686429 2312 58-59 -0.05964095526079305 2312 60-61 0.12067840600451873 2312 62-63 -0.23707914042898537 2312 64-65 -0.06420516926372954 2312 66-67 -0.15772560674531988 2312 68-69 -0.3938992721622583 2312 70-71 -0.543557296482259 2312 72-73 -0.16039345856784237 2312 74-75 -0.14620470516024398 2312 76-77 0.19623332315965314 2312 78-79 0.17712044827671392 2312 80-81 0.2627990660466928 2312 82-83 0.13207272000784087 2312 84-85 0.23746646024028806 2312 86-87 0.46767478400467155 2312 88-89 0.8303097173351723 2312 90-91 1.524566184775189 2312 92-93 2.1838061980134373 2312 94-95 1.9021947303244335 2312 96-97 1.1819225397450772 2312 98-99 0.9766589679013773 2312 100-101 0.8518048522133448 2313 1 -6.998659382547739 2313 2 -2.762793967090836 2313 3 -1.674655512936777 2313 4 -1.0706450207398461 2313 5 -0.7946679848982967 2313 6 -0.2553878080509904 2313 7 -0.5219199249180235 2313 8 -0.1516264640608469 2313 9 -0.003609207337667897 2313 10-11 -0.10880570640890141 2313 12-13 -0.42398986193447996 2313 14-15 -0.39202484004993465 2313 16-17 -0.5016365997833958 2313 18-19 -0.6100597865737569 2313 20-21 -0.628179957534023 2313 22-23 -1.2338343664869171 2313 24-25 -0.5937143825929567 2313 26-27 -0.4310865679831366 2313 28-29 -0.574018571982009 2313 30-31 -0.047219644379723036 2313 32-33 -0.21653833573483183 2313 34-35 -0.008153129246416313 2313 36-37 0.33732432585653527 2313 38-39 0.1751156920479815 2313 40-41 -0.1636739410687582 2313 42-43 -0.06668562879670148 2313 44-45 -0.14135602682474158 2313 46-47 -0.9871380816561377 2313 48-49 -0.5014612133167233 2313 50-51 -0.30377084051675496 2313 52-53 -0.7308407890017641 2313 54-55 -1.0675134527172965 2313 56-57 -0.5386119176923003 2313 58-59 -0.611791492895204 2313 60-61 -0.38577320689870476 2313 62-63 -0.5795522587085564 2313 64-65 -0.26581807248953737 2313 66-67 -0.19804818739048002 2313 68-69 -0.02024335818376244 2313 70-71 -0.03172933949300827 2313 72-73 -0.2206085123312853 2313 74-75 -0.15480685569787767 2313 76-77 0.06558816186933214 2313 78-79 -0.016427938820061883 2313 80-81 0.3644119692724992 2313 82-83 0.3482017522659042 2313 84-85 0.38746646024028664 2313 86-87 0.6762769345423081 2313 88-89 0.9776215452921626 2313 90-91 1.2831683353128227 2313 92-93 2.5375696388736504 2313 94-95 2.43929150451798 2313 96-97 1.4953633999601301 2313 98-99 0.951927785105676 2313 100-101 0.9270736694176467 2314 1 0.7567140864879676 2314 2 0.33510149607976913 2314 3 -1.0266651731058296 2314 4 0.05318339625745239 2314 5 -0.13327416050637453 2314 6 -2.9825570335124354 2314 7 -2.3853895356362074 2314 8 -2.3181666285137297 2314 9 -3.431715699201277 2314 10-11 -2.4725196964037224 2314 12-13 -1.0979217807430572 2314 14-15 -0.8662931397451743 2314 16-17 -2.33530918905371 2314 18-19 -3.039408300172738 2314 20-21 -2.8472789167658092 2314 22-23 -4.122871799641992 2314 24-25 -3.1351611079106547 2314 26-27 -1.6362702836981597 2314 28-29 -1.6914471269817284 2314 30-31 -1.8023458590884758 2314 32-33 -2.0628467711324454 2314 34-35 -1.5046628181659685 2314 36-37 -1.8391146368253146 2314 38-39 -3.588210166154539 2314 40-41 -2.3019264854882877 2314 42-43 -1.780597997263147 2314 44-45 -1.9572493049571094 2314 46-47 -1.7892857442402352 2314 48-49 -2.844463340853956 2314 50-51 -3.6321648374117004 2314 52-53 -2.2838164660761144 2314 54-55 -2.433915614755133 2314 56-57 -2.075815068747442 2314 58-59 -1.486491050137456 2314 60-61 -1.8794394710583298 2314 62-63 -1.8775713490426398 2314 64-65 -2.5542459949781815 2314 66-67 -1.849873844339477 2314 68-69 -1.963561741255468 2314 70-71 -1.6994396494234323 2314 72-73 -2.5296016697115355 2314 74-75 -2.396624462505997 2314 76-77 -1.8408577009332667 2314 78-79 -2.8031193309194222 2314 80-81 -1.760874098233682 2314 82-83 -1.2388490211226326 2314 84-85 -2.2302812253442106 2314 86-87 -0.7562916354076634 2314 88-89 0.33290301271784273 2314 90-91 1.2499730043945334 2314 92-93 2.017099280642382 2314 94-95 1.7174037464822138 2314 96-97 0.7650114938017722 2314 98-99 0.728231620422795 2314 100-101 0.7883122410926511 2315 1 -2.967050761365897 2315 2 -2.084439699320356 2315 3 -2.7212584247266705 2315 4 -4.418270865254069 2315 5 -4.220659991749571 2315 6 -3.261917925093524 2315 7 -6.115958368157148 2315 8 -3.206805358341942 2315 9 -2.891602831849994 2315 10-11 -3.7576138708393856 2315 12-13 -2.885431245510457 2315 14-15 -1.954090688370421 2315 16-17 -2.360136457048597 2315 18-19 -2.0832982012659187 2315 20-21 -1.2868534754710268 2315 22-23 -0.6317325965754037 2315 24-25 -2.0962431674440367 2315 26-27 -3.7805299022680323 2315 28-29 -3.0712114542733815 2315 30-31 -3.1385651577492126 2315 32-33 -3.653538763939224 2315 34-35 -3.5219472580883604 2315 36-37 -3.076230722197515 2315 38-39 -1.9003374909380284 2315 40-41 -1.4664572696442661 2315 42-43 -2.7415286205180784 2315 44-45 -6.114283993329643 2315 46-47 -2.751361604350972 2315 48-49 -1.3786997707436903 2315 50-51 -2.320196047482213 2315 52-53 -2.4850800125244596 2315 54-55 -1.2343858953854863 2315 56-57 -0.9220392181014709 2315 58-59 -2.106051175072384 2315 60-61 -2.3314531003233867 2315 62-63 -3.867971708703802 2315 64-65 -3.480268781405705 2315 66-67 -2.959136302339573 2315 68-69 -1.4602721430348424 2315 70-71 -2.6306148186061513 2315 72-73 -1.4360678804919083 2315 74-75 -0.5217126031524408 2315 76-77 -2.2363589786768685 2315 78-79 -0.9905501198077857 2315 80-81 -0.8463835393391648 2315 82-83 -0.49268225192098214 2315 84-85 -2.065421540520962 2315 86-87 -1.5884925487577206 2315 88-89 -1.2611820992030331 2315 90-91 -0.635309873841237 2315 92-93 -0.21184752736481194 2315 94-95 -0.09321039861267089 2315 96-97 -0.25289880386516295 2315 98-99 -0.4488346566109467 2315 100-101 -1.016224931781327 2316 1 -7.969809373435293 2316 2 -6.834873427633941 2316 3 -3.6914224165270824 2316 4 -4.168968330380814 2316 5 -2.0304070043989384 2316 6 -2.770496246177469 2316 7 -3.225765377806667 2316 8 -2.2980270472574986 2316 9 -7.1973762931769265 2316 10-11 -6.804677767644545 2316 12-13 -5.705755854280021 2316 14-15 -4.763812702269725 2316 16-17 -5.027789324405234 2316 18-19 -4.245124484951738 2316 20-21 -2.6132446559120055 2316 22-23 -3.1471112026451067 2316 24-25 -3.1171333729337647 2316 26-27 -4.581232367144793 2316 28-29 -4.402084910600564 2316 30-31 -7.029268122600968 2316 32-33 -4.907289201417349 2316 34-35 -5.967329363983062 2316 36-37 -4.565236271920028 2316 38-39 -6.665124876568747 2316 40-41 -3.8095551147520084 2316 42-43 -4.908976498124201 2316 44-45 -3.1648114669559604 2316 46-47 -3.919259459458214 2316 48-49 -5.355853413061574 2316 50-51 -4.482939785295322 2316 52-53 -5.820343249362619 2316 54-55 -5.166638657747367 2316 56-57 -4.587691560484355 2316 58-59 -3.225241465557861 2316 60-61 -3.1104041527707658 2316 62-63 -4.248670173780546 2316 64-65 -3.123308504419551 2316 66-67 -3.8476381272483238 2316 68-69 -2.6637188456997123 2316 70-71 -2.072653341679999 2316 72-73 -1.6921594509133868 2316 74-75 -1.601817972883957 2316 76-77 -2.3940254703522825 2316 78-79 -4.0005996173329095 2316 80-81 -4.662387739129176 2316 82-83 -2.512778747096224 2316 84-85 -1.2516897271116356 2316 86-87 -1.4196953636169773 2316 88-89 -0.3790943285915631 2316 90-91 -0.397804874091225 2316 92-93 -0.3288054294697069 2316 94-95 -0.7887474967577628 2316 96-97 -2.1945673454380845 2316 98-99 -3.608633541666693 2316 100-101 -3.3755507154921407 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10611.0 3 2341.0 4 1940.0 5 2068.0 6 2478.0 7 3101.0 8 4116.0 9 5128.0 10 6544.0 11 8304.0 12 10939.0 13 13249.0 14 15373.0 15 17882.0 16 20689.0 17 21248.0 18 16573.0 19 13939.0 20 14499.0 21 15145.0 22 16308.0 23 17910.0 24 19374.0 25 21452.0 26 23564.0 27 25755.0 28 28410.0 29 31658.0 30 34571.0 31 38571.0 32 42471.0 33 47430.0 34 53153.0 35 58191.0 36 58856.0 37 50043.0 38 15178.0 39 219.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.78507531612728 21.867621740879027 14.528216429357114 17.819086513636577 2 15.529161338772859 23.757712760559375 40.366787432259635 20.346338468408128 3 18.579149011747393 32.579859437241524 30.796691446984497 18.04430010402659 4 11.083437584849738 19.596324594814746 39.23223516097026 30.088002659365255 5 10.762829051696796 41.62600697748176 32.49844592451633 15.112718046305107 6 24.34308523461151 40.21802845636767 19.158565458019623 16.2803208510012 7 24.203933215298797 38.82295454401228 21.370850546702965 15.60226169398595 8 26.05875716825524 32.577602430385916 20.85662474318643 20.507015658172413 9 29.60505023968078 16.86357677082659 21.288247709484303 32.24312528000833 10-11 23.39005657518357 29.388130125460354 26.260441281355572 20.961372018000503 12-13 27.114306017308326 28.712217343857066 26.953023881430347 17.22045275740426 14-15 24.119281085148746 28.354701207201334 28.7543098470726 18.77170786057732 16-17 18.432818633500176 32.15916971263139 29.103074684876685 20.30493696899175 18-19 17.908184179900424 29.25380767995817 35.39153014098664 17.446477999154766 20-21 20.217551746436275 27.814030761342266 34.8501626783449 17.118254813876554 22-23 19.717436294612817 28.28112898515763 34.07219309048895 17.92924162974061 24-25 18.461740674233784 29.199737885456962 35.1002994506262 17.23822198968305 26-27 18.56173304823457 28.83436985404459 35.40753902908503 17.19635806863581 28-29 17.70433859329268 28.571047553004426 36.19579774780009 17.5288161059028 30-31 17.880672755534334 29.967185134764456 35.23064380124148 16.921498308459736 32-33 17.201427949145504 30.146376217103132 34.77857517496949 17.873620658781874 34-35 17.79559164040829 29.421168684482364 34.89863515703916 17.884604518070184 36-37 18.26988222012119 29.1322488534098 34.443296566674306 18.154572359794702 38-39 18.171527322779056 29.28678632921422 34.724804075434136 17.81688227257259 40-41 17.327812969637787 29.46971366023351 35.37187217661778 17.83060119351093 42-43 17.14542201703229 29.890370675107896 34.29147890390991 18.672728403949908 44-45 17.3594191011507 29.977006298826698 33.69548518487012 18.96808941515248 46-47 17.574741317296073 29.861669753441113 32.683766783343984 19.879822145918823 48-49 17.74610098998078 29.91277803114127 32.171961681277764 20.16915929760019 50-51 18.082805553738243 30.24950408514428 31.837511425255805 19.830178935861678 52-53 17.611656454967104 30.45692856298965 31.815594872257417 20.11582010978583 54-55 17.40936822147342 30.001810253657823 31.834485649769906 20.75433587509885 56-57 18.094348476833893 30.444652868751742 30.602389287639724 20.858609366774637 58-59 17.810614816037795 29.399535173167045 31.1853719249673 21.60447808582786 60-61 18.239927173603224 29.515512746206486 30.968676142816427 21.27588393737387 62-63 18.963513296486283 28.970426085526878 31.14931941719285 20.916741200793986 64-65 18.576444322846807 28.80183936881516 31.48897083328308 21.132745475054957 66-67 18.244072823675946 29.150166137422374 31.063000173945944 21.54276086495573 68-69 18.31861266627011 29.172250532146876 31.13070339882036 21.378433402762656 70-71 18.177708787668124 29.089235320819885 30.897114795521567 21.835941095990428 72-73 18.110215217656382 28.66863289029793 30.493359549648417 22.72779234239727 74-75 17.742868665313914 28.95288540009463 30.655577889766377 22.64866804482508 76-77 17.941168810792853 28.339116852495174 31.26886154553672 22.450852791175258 78-79 17.794165285161682 27.820461517940508 31.450421438416637 22.93495175848117 80-81 17.598248198552742 28.72437269359801 30.742031736472548 22.935347371376697 82-83 17.945654072768274 28.109009481156793 30.34605428240189 23.599282163673035 84-85 17.7790239741796 27.423313935432493 30.32791830653341 24.469743783854504 86-87 18.12934616857593 27.361181414768577 30.631386474218473 23.878085942437025 88-89 18.737972112449555 26.545698668627963 30.575255356419255 24.141073862503223 90-91 18.43870396226487 26.68473577900614 30.78172351076192 24.094836747967065 92-93 19.04464168486318 26.663530875433878 30.54598459383218 23.74584284587076 94-95 18.53821438606886 26.34148636294862 30.606537596456185 24.513761654526338 96-97 18.69366650443552 25.9825679877704 29.814357583713182 25.509407924080907 98-99 18.82853910589671 25.70769406769966 29.741010029108566 25.722756797295066 100-101 19.48158116726877 25.401709119180012 30.40536116473872 24.7113485488125 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3921.0 1 2710.0 2 1650.0 3 1765.0 4 1506.5 5 1493.0 6 2347.5 7 4368.0 8 6403.0 9 6667.0 10 5391.0 11 4190.0 12 3808.5 13 3714.5 14 3495.0 15 3200.5 16 3070.5 17 2977.0 18 2960.5 19 3106.0 20 3228.0 21 3408.0 22 3802.5 23 4200.5 24 4573.0 25 5172.5 26 6195.0 27 7424.5 28 8258.0 29 9280.5 30 11128.5 31 13062.5 32 15911.5 33 19461.0 34 22672.0 35 25626.5 36 28452.5 37 30943.5 38 31158.5 39 29928.0 40 28759.5 41 27396.0 42 26131.5 43 26144.5 44 26492.0 45 25963.0 46 25183.5 47 25419.0 48 30321.5 49 30036.0 50 22690.5 51 17600.0 52 14072.5 53 11964.0 54 10865.0 55 10287.5 56 9864.0 57 9578.5 58 9175.0 59 8510.0 60 8003.0 61 7359.5 62 6229.5 63 4669.0 64 3243.5 65 2563.0 66 2113.0 67 1654.0 68 1276.0 69 973.5 70 748.5 71 585.5 72 503.0 73 508.5 74 479.0 75 384.0 76 279.0 77 180.0 78 122.5 79 87.0 80 61.5 81 41.5 82 24.5 83 18.5 84 14.0 85 7.0 86 5.5 87 5.0 88 4.0 89 2.0 90 2.5 91 2.0 92 1.5 93 1.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13556642057771567 2 0.12378354477049365 3 0.12935823870079224 4 0.14228139281193897 5 0.13062521459404192 6 0.14278818316923883 7 0.17433588291115584 8 0.15938556737080964 9 0.17205532630330642 10-11 0.16508695889043318 12-13 0.15469775656578583 14-15 0.15963896254945958 16-17 0.15412761741382347 18-19 0.1690145841595072 20-21 0.17439923170581834 22-23 0.21171167176202138 24-25 0.23299686676861597 26-27 0.2472503455676749 28-29 0.24294262753062598 30-31 0.2322366812326662 32-33 0.2354041209657904 34-35 0.22229092047065618 36-37 0.23280682038462852 38-39 0.2195669223001694 40-41 0.2245714770785056 42-43 0.21766645846029486 44-45 0.21209176452999629 46-47 0.22571175538243035 48-49 0.26530475204648285 50-51 0.26600158878777014 52-53 0.2675853086543322 54-55 0.26587489119844515 56-57 0.23964849020817683 58-59 0.23958514141351434 60-61 0.2096211615381594 62-63 0.2040464676078608 64-65 0.20518674591178554 66-67 0.21285195006594612 68-69 0.21152162537803393 70-71 0.21899678314820703 72-73 0.2514947148100613 74-75 0.2532051322659484 76-77 0.2699292140568441 78-79 0.26555814722513277 80-81 0.2794315332562167 82-83 0.2779111621843171 84-85 0.25295173708729846 86-87 0.2561191768204226 88-89 0.2594766629375343 90-91 0.4553511360339347 92-93 0.6068814528665963 94-95 0.3527260886807107 96-97 0.296218963841775 98-99 0.3257395021544925 100-101 0.3465812555984497 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 789281.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.28080400792922 #Duplication Level Percentage of deduplicated Percentage of total 1 83.5553688045374 36.163435410394264 2 7.153184088203113 6.191911171083135 3 2.7037140079958872 3.5105674822068824 4 1.4592935791273363 2.5263759755295916 5 0.9331085672453712 2.0192844508533274 6 0.6480411702969131 1.6828645728413865 7 0.49919974622621743 1.5124036464057442 8 0.3580867401422654 1.2398625614348533 9 0.2775403471910685 1.081095243396234 >10 2.0136111080210592 17.454686073191542 >50 0.24875602492121276 7.385306677429646 >100 0.14047043309635554 10.513538652018289 >500 0.004511898279325877 1.2936555336325979 >1k 0.003910311842082426 2.910562218519069 >5k 9.023796558651753E-4 2.8662990725559934 >10k+ 3.007932186217251E-4 1.648151258507466 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12660 1.6039914808540938 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9210 1.1668847976829546 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6954 0.8810550361658268 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5853 0.7415609903190372 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3509 0.44458184094131237 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2566 0.32510601420786767 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2213 0.280381765176154 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2046 0.2592232677588843 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1677 0.21247185729797122 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1628 0.20626367542104776 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1511 0.19144005747002651 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1417 0.17953048407347955 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1275 0.16153942638933408 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1195 0.15140361924333667 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1156 0.14646241325966292 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1155 0.14633571567033793 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1009 0.12783786762889263 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 982 0.12441703271711849 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 834 0.10566578949702324 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 798 0.1011046762813244 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.2669758932496792E-4 0.0 3 0.0 0.0 0.0 1.2669758932496792E-4 0.0 4 0.0 0.0 0.0 1.2669758932496792E-4 0.0 5 0.0 0.0 0.0 1.2669758932496792E-4 0.0 6 0.0 0.0 0.0 1.2669758932496792E-4 0.0 7 0.0 0.0 0.0 1.2669758932496792E-4 0.0 8 0.0 0.0 0.0 1.2669758932496792E-4 0.0 9 0.0 0.0 0.0 1.2669758932496792E-4 0.0 10-11 0.0 0.0 0.0 1.900463839874519E-4 0.0 12-13 0.0 0.0 0.0 5.067903572998717E-4 0.0 14-15 0.0 0.0 0.0 5.067903572998717E-4 0.0 16-17 0.0 0.0 0.0 0.0011402783039247111 0.0 18-19 0.0 0.0 0.0 0.001710417455887067 0.0 20-21 0.0 0.0 0.0 0.0040543228583989725 0.0 22-23 0.0 0.0 0.0 0.014950315540346214 0.0 24-25 0.0 0.0 0.0 0.03287802442982918 0.0 26-27 0.0 0.0 0.0 0.06220851635855924 0.0 28-29 0.0 0.0 0.0 0.1144712719551085 0.0 30-31 0.0 0.0 0.0 0.280381765176154 0.0 32-33 0.0 0.0 0.0 0.4884192068477513 0.0 34-35 0.0 0.0 0.0 0.779506918321865 0.0 36-37 0.0 0.0 0.0 1.1827219963485756 0.0 38-39 0.0 0.0 0.0 1.71738582329994 0.0 40-41 0.0 0.0 0.0 2.3101404949568023 0.0 42-43 0.0 0.0 0.0 2.8671030976293617 0.0 44-45 0.0 0.0 0.0 3.4317942532507435 0.0 46-47 0.0 0.0 0.0 4.093219018321738 0.0 48-49 0.0 0.0 0.0 4.814191650375468 0.0 50-51 0.0 0.0 0.0 5.550177946764206 0.0 52-53 0.0 0.0 0.0 6.197475930625468 0.0 54-55 0.0 0.0 0.0 6.829950296535708 0.0 56-57 0.0 0.0 0.0 7.497583243483627 0.0 58-59 0.0 0.0 0.0 8.248709901796698 0.0 60-61 0.0 0.0 0.0 9.116968481440704 0.0 62-63 0.0 0.0 0.0 9.891534193778895 0.0 64-65 0.0 0.0 0.0 10.596669627167003 0.0 66-67 0.0 0.0 0.0 11.356094977580861 0.0 68-69 0.0 0.0 0.0 12.141429985011676 0.0 70-71 0.0 0.0 0.0 13.027806319929152 0.0 72-73 0.0 0.0 0.0 13.892200623098745 0.0 74-75 0.0 0.0 0.0 14.696476920133641 0.0 76-77 0.0 0.0 0.0 15.499232846096637 0.0 78-79 0.0 0.0 0.0 16.338350980195898 0.0 80-81 0.0 0.0 0.0 17.194383242470046 0.0 82-83 0.0 0.0 0.0 18.002587164774017 0.0 84-85 0.0 0.0 0.0 18.82156038217061 0.0 86-87 0.0 0.0 0.0 19.6395200188526 0.0 88-89 0.0 0.0 0.0 20.401542669847622 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGATAGT 145 0.0 39.261734 6 CGTCACT 190 0.0 34.95672 1 CATATAG 190 0.0 34.9545 3 GCGCAGA 170 3.6379788E-12 33.48795 1 GTCAACG 85 1.645429E-5 33.48795 1 GACATAT 195 0.0 31.627508 1 GGGACCG 90 2.4324921E-5 31.627504 9 TGGGAGT 330 0.0 30.189892 6 AACGGAG 95 3.5205852E-5 29.961 5 CATGGGT 540 0.0 28.111557 4 GCCTATC 85 6.451888E-4 27.906626 7 GATAGTC 170 3.6852725E-9 27.906626 7 GTACAGG 155 4.6389687E-8 27.546537 1 CATGGGG 2145 0.0 26.981197 4 GTATCAA 14940 0.0 26.927837 1 GTACATG 9805 0.0 26.660004 1 TACATGG 9920 0.0 26.397093 2 GTTCGAC 100 7.2577677E-10 26.103462 16-17 ATGGGAT 2125 0.0 25.895704 5 ACATGGG 10050 0.0 25.253197 3 >>END_MODULE