##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939809_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 789281 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.32668593314675 31.0 31.0 33.0 30.0 34.0 2 31.475017135848955 31.0 31.0 34.0 30.0 34.0 3 31.4780122668606 31.0 31.0 34.0 30.0 34.0 4 35.343673545923444 37.0 35.0 37.0 33.0 37.0 5 35.10614977428825 37.0 35.0 37.0 32.0 37.0 6 35.18911135577823 37.0 35.0 37.0 32.0 37.0 7 35.09587586676988 37.0 35.0 37.0 32.0 37.0 8 35.05718749089361 37.0 35.0 37.0 32.0 37.0 9 36.55717545462262 39.0 35.0 39.0 32.0 39.0 10-11 36.42312433721324 38.0 35.0 39.0 32.0 39.0 12-13 36.359585496166765 38.0 35.0 39.0 32.0 39.0 14-15 37.10465791017394 39.0 36.0 40.5 31.0 41.0 16-17 37.0942021916149 39.0 36.0 40.0 31.5 41.0 18-19 37.30755839808636 39.0 36.0 40.0 32.0 41.0 20-21 37.33102405860524 39.0 36.0 41.0 32.0 41.0 22-23 37.160753267847575 39.0 36.0 40.5 31.0 41.0 24-25 36.939067328365944 39.0 35.5 40.0 31.0 41.0 26-27 36.542245410696566 38.0 35.0 40.0 30.0 41.0 28-29 36.239288669054496 38.0 35.0 40.0 30.0 41.0 30-31 35.7638756032389 38.0 34.5 40.0 29.5 41.0 32-33 35.36506516690507 37.5 34.0 40.0 29.0 41.0 34-35 35.11952017088971 38.0 34.0 40.0 27.5 41.0 36-37 34.848279636783346 38.0 33.5 40.0 26.0 41.0 38-39 34.52296140411336 37.0 33.0 40.0 25.0 41.0 40-41 34.15080750708556 37.0 33.0 40.0 24.0 41.0 42-43 33.68683586707396 37.0 33.0 40.0 22.0 41.0 44-45 33.09850990965195 37.0 33.0 40.0 17.5 41.0 46-47 32.37815556183412 37.0 32.0 40.0 5.5 41.0 48-49 31.79283423774296 36.0 31.0 40.0 2.0 41.0 50-51 31.375467672476596 36.0 31.0 40.0 2.0 41.0 52-53 31.004964011549752 36.0 30.0 40.0 2.0 41.0 54-55 30.65688962486111 35.0 30.0 39.0 2.0 41.0 56-57 29.633116089200172 35.0 27.0 39.0 2.0 40.5 58-59 27.038528736913722 33.5 17.0 38.0 2.0 40.0 60-61 26.841406926050418 33.0 17.5 38.0 2.0 40.0 62-63 28.024042134550307 34.0 25.0 38.0 2.0 40.0 64-65 27.42824165284607 34.0 22.0 38.0 2.0 40.0 66-67 22.685013955739464 26.0 10.0 35.0 2.0 38.5 68-69 15.844462871904938 12.5 2.0 28.5 2.0 35.0 70-71 14.867990614242583 10.5 2.0 25.0 2.0 33.0 72-73 16.831779176237614 14.5 2.0 29.0 2.0 34.0 74-75 17.451453284698353 16.5 2.0 30.0 2.0 35.0 76-77 18.239958265814074 20.5 2.0 30.5 2.0 34.5 78-79 20.9237597256237 27.0 2.0 33.5 2.0 35.5 80-81 22.983664246320387 30.5 2.0 34.0 2.0 36.0 82-83 23.510385401396967 31.0 2.0 35.0 2.0 36.0 84-85 23.367153143177145 32.0 2.0 35.0 2.0 35.5 86-87 23.032529606059185 31.0 2.0 35.0 2.0 35.0 88-89 22.57564732965826 31.0 2.0 34.0 2.0 35.0 90-91 21.298858074627415 30.0 2.0 34.0 2.0 35.0 92-93 19.74655034138665 28.0 2.0 34.0 2.0 35.0 94-95 16.073174192714635 7.0 2.0 32.0 2.0 35.0 96-97 14.996132556085856 2.0 2.0 31.5 2.0 34.5 98-99 14.072427310425564 2.0 2.0 31.0 2.0 34.0 100-101 13.547191684583819 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.28494009565642386 1101 2 0.23329700336452674 1101 3 0.516485703598061 1101 4 0.22169447028784361 1101 5 0.5299895329862068 1101 6 0.3012337648224701 1101 7 0.5771102576883322 1101 8 0.46002024509365214 1101 9 0.7962884481232777 1101 10-11 0.8113132318253164 1101 12-13 0.4901937228289128 1101 14-15 0.3811181507251007 1101 16-17 0.39122386559219535 1101 18-19 0.4527192598332519 1101 20-21 0.6151639092800494 1101 22-23 0.4304587283080039 1101 24-25 0.18212674453675248 1101 26-27 0.22290201190694603 1101 28-29 0.3551183886807294 1101 30-31 0.6256371989648812 1101 32-33 0.6413764030395583 1101 34-35 0.8518335237206003 1101 36-37 0.43684069935260794 1101 38-39 0.17825134967576162 1101 40-41 0.8112792104037894 1101 42-43 0.5915820395460045 1101 44-45 0.31971380898528423 1101 46-47 0.4204772787518678 1101 48-49 0.9750412621896913 1101 50-51 0.7484277589023485 1101 52-53 0.8957902754698246 1101 54-55 1.0176982228524487 1101 56-57 1.5487208481067647 1101 58-59 -6.900551274546242 1101 60-61 -7.625197753989955 1101 62-63 -1.4505301172417084 1101 64-65 0.35531371955037727 1101 66-67 -1.3955829811095057 1101 68-69 -4.054778696627221 1101 70-71 -3.8530951681589105 1101 72-73 -6.300559372585385 1101 74-75 -6.725725430878626 1101 76-77 -8.00897918904076 1101 78-79 -10.126939184038488 1101 80-81 -5.99560465768878 1101 82-83 -3.2005924983300176 1101 84-85 -2.378163001198626 1101 86-87 -2.1855064378335847 1101 88-89 -2.921778026399668 1101 90-91 -7.753276518084835 1101 92-93 -12.3890359501152 1101 94-95 -10.699465459932181 1101 96-97 -10.328575977637634 1101 98-99 -10.015932672693918 1101 100-101 -9.927010022861994 1102 1 0.5063275480908089 1102 2 0.49005376012128465 1102 3 0.5790649796249916 1102 4 0.22957328773345154 1102 5 0.5253305418651237 1102 6 0.40824420486540447 1102 7 0.5046519690748141 1102 8 0.3881473794482204 1102 9 0.5084432924983417 1102 10-11 0.7199079727756583 1102 12-13 0.6467394833909168 1102 14-15 0.9104441825330838 1102 16-17 0.9677801149823821 1102 18-19 0.9197038436950891 1102 20-21 0.8851353699103512 1102 22-23 0.8619276521054431 1102 24-25 0.8404104795352936 1102 26-27 1.1154890935734514 1102 28-29 1.0251349756648196 1102 30-31 0.9213184849439955 1102 32-33 1.1674643140552732 1102 34-35 1.0828621606607882 1102 36-37 1.2909423190228253 1102 38-39 1.1179466858061105 1102 40-41 1.0403625356062491 1102 42-43 1.1704372937171499 1102 44-45 1.2163378698692782 1102 46-47 1.4381680290689687 1102 48-49 1.2876205382166148 1102 50-51 1.2670027418275147 1102 52-53 1.3144652681520448 1102 54-55 0.9685165953765953 1102 56-57 1.6050251216944353 1102 58-59 -7.975741537010876 1102 60-61 -8.745685118610893 1102 62-63 -2.2011643254571425 1102 64-65 -0.09194942807378226 1102 66-67 -0.9843599349586647 1102 68-69 -2.7922591825282836 1102 70-71 -3.696360364763457 1102 72-73 -5.7870458590718705 1102 74-75 -6.312288754129675 1102 76-77 -6.793781364862792 1102 78-79 -9.535168035633767 1102 80-81 -5.959820678764011 1102 82-83 -2.2413342780158416 1102 84-85 -1.6465672357580914 1102 86-87 -1.5005737614749144 1102 88-89 -1.3337816365079718 1102 90-91 -6.387161531256851 1102 92-93 -11.783098785513776 1102 94-95 -10.156802761132298 1102 96-97 -10.075484212587385 1102 98-99 -9.800936087661231 1102 100-101 -9.75187708306299 1103 1 0.2575385601717457 1103 2 0.36649717813514116 1103 3 0.28665454449438244 1103 4 0.14724530981096962 1103 5 0.1370521383167116 1103 6 0.1277271172973471 1103 7 0.07725850061948591 1103 8 0.31367326300763665 1103 9 0.13230835948961328 1103 10-11 0.3616339046921837 1103 12-13 0.4279589996530788 1103 14-15 0.4952066907662953 1103 16-17 0.5047545440766896 1103 18-19 0.313409915847231 1103 20-21 0.41659250300078554 1103 22-23 0.31382275052883557 1103 24-25 0.5604895183960679 1103 26-27 0.31828463471450874 1103 28-29 0.2814026406000849 1103 30-31 0.5134992238072726 1103 32-33 0.5134427534546404 1103 34-35 0.32718228472178623 1103 36-37 0.08801337896063188 1103 38-39 0.20553975239719335 1103 40-41 -0.0509406884789243 1103 42-43 -0.18530844793917112 1103 44-45 -0.33669028564051473 1103 46-47 -0.4398115608960964 1103 48-49 0.05872049569571658 1103 50-51 0.09501192967820415 1103 52-53 -0.07036352267011381 1103 54-55 0.08490298535166474 1103 56-57 0.5147849202620627 1103 58-59 -5.506404529648922 1103 60-61 -7.465555721657392 1103 62-63 -2.3976776111559452 1103 64-65 -0.5837662380053352 1103 66-67 -1.6741782623029415 1103 68-69 -3.917985581341034 1103 70-71 -4.395781330071399 1103 72-73 -6.851710986092655 1103 74-75 -7.2801813012987004 1103 76-77 -7.775484869085702 1103 78-79 -10.47869235175586 1103 80-81 -7.277203808802945 1103 82-83 -4.014606136679689 1103 84-85 -3.14382213608409 1103 86-87 -2.9231398564716216 1103 88-89 -3.0513969640440095 1103 90-91 -7.5643799136281835 1103 92-93 -11.672335993807854 1103 94-95 -10.368665260194337 1103 96-97 -10.21563436974799 1103 98-99 -9.924271727684243 1103 100-101 -9.813678301995632 1104 1 0.24893512516869265 1104 2 0.21763996701783483 1104 3 0.3725667846791403 1104 4 0.2803928238137843 1104 5 0.3196913037224576 1104 6 0.1915879120731674 1104 7 0.3187147932583869 1104 8 0.2910935597876545 1104 9 0.21738972181076122 1104 10-11 0.3330164005112479 1104 12-13 0.279790647339631 1104 14-15 0.44268618427589246 1104 16-17 0.44199273791279836 1104 18-19 0.3159609497990772 1104 20-21 0.21290000744718185 1104 22-23 0.2705255192368554 1104 24-25 0.3544318082211291 1104 26-27 0.3000610675142781 1104 28-29 0.4417912684570524 1104 30-31 0.31231396193474836 1104 32-33 0.4987311716012286 1104 34-35 0.6351008427637836 1104 36-37 0.8396870802162368 1104 38-39 0.847236282884829 1104 40-41 0.6672204965174942 1104 42-43 0.6688459413788763 1104 44-45 0.6566507459222208 1104 46-47 0.3636234421566513 1104 48-49 0.550662573155833 1104 50-51 0.544956183879048 1104 52-53 0.41018915711008574 1104 54-55 0.30555547665797533 1104 56-57 1.1045728207690857 1104 58-59 -5.953479202474174 1104 60-61 -7.716289708012944 1104 62-63 -1.8882592256604696 1104 64-65 -0.053431232298024156 1104 66-67 -1.4283804337345458 1104 68-69 -3.796767668357571 1104 70-71 -4.462049812458382 1104 72-73 -6.760609077462675 1104 74-75 -7.844788027958465 1104 76-77 -7.995671484784779 1104 78-79 -10.705942756576544 1104 80-81 -7.518204844082071 1104 82-83 -4.207951150085222 1104 84-85 -3.3825044115745158 1104 86-87 -3.257462014099506 1104 88-89 -3.3680423942157596 1104 90-91 -7.8681918644656985 1104 92-93 -12.381673415166459 1104 94-95 -10.297818985871913 1104 96-97 -10.166270137314552 1104 98-99 -9.664845378503177 1104 100-101 -9.187009246223287 1105 1 0.001987239888034509 1105 2 0.1486298119012197 1105 3 0.1440878202778464 1105 4 0.1424283109246005 1105 5 0.12158841342003512 1105 6 0.14932700369240592 1105 7 0.15953613016274915 1105 8 0.13559752139908454 1105 9 0.2645273180611767 1105 10-11 0.11993192333235925 1105 12-13 0.14996696694681333 1105 14-15 0.33089620547888643 1105 16-17 0.2545471403232398 1105 18-19 0.22505591687864523 1105 20-21 0.24211549678988575 1105 22-23 0.21908594776045476 1105 24-25 0.43225794369708126 1105 26-27 0.37650342885788035 1105 28-29 0.1198126946371687 1105 30-31 0.15174036523794854 1105 32-33 0.17936949321711637 1105 34-35 0.27034244526350193 1105 36-37 0.15309518199058658 1105 38-39 0.38416296517475246 1105 40-41 0.5540523233225372 1105 42-43 0.20370812193907994 1105 44-45 0.3602106164723864 1105 46-47 0.11393590728331304 1105 48-49 0.4117048675426176 1105 50-51 0.09664126366925174 1105 52-53 0.060685753472100146 1105 54-55 0.25966774091419964 1105 56-57 0.7596726980149278 1105 58-59 -6.050566581114946 1105 60-61 -6.923336912566004 1105 62-63 -1.8936771477673773 1105 64-65 0.11320753569662045 1105 66-67 -1.5088625227871084 1105 68-69 -3.7076202517745998 1105 70-71 -4.036522527557144 1105 72-73 -4.859052331563781 1105 74-75 -5.209167003519227 1105 76-77 -6.901915207583574 1105 78-79 -10.159296176953735 1105 80-81 -6.6459807619483815 1105 82-83 -2.983004157562064 1105 84-85 -2.3185439160941037 1105 86-87 -2.1636945774294922 1105 88-89 -2.0653864406391484 1105 90-91 -6.571472748295626 1105 92-93 -8.849260738009892 1105 94-95 -9.826509980180585 1105 96-97 -10.025839381818514 1105 98-99 -9.419348224167722 1105 100-101 -9.138158671510643 1106 1 0.13942624324602093 1106 2 0.21575908927488996 1106 3 0.10422822668541087 1106 4 0.1428797621627922 1106 5 0.20433080215506294 1106 6 0.07622741050577275 1106 7 0.31735638155515034 1106 8 0.2333088157960148 1106 9 0.2856237865965454 1106 10-11 0.06953677668679603 1106 12-13 0.034806321320822065 1106 14-15 0.3384542093542606 1106 16-17 0.3227137410475862 1106 18-19 0.12296199473115621 1106 20-21 0.017079735764838233 1106 22-23 0.31085989750227583 1106 24-25 0.04152689704035595 1106 26-27 0.3154738156856496 1106 28-29 0.24508280450721287 1106 30-31 0.32349473518448235 1106 32-33 0.3701000326252597 1106 34-35 0.24931191904382644 1106 36-37 0.4461656591086083 1106 38-39 0.3117608387886932 1106 40-41 0.365809838210275 1106 42-43 0.5748543008355114 1106 44-45 0.6424919162461578 1106 46-47 0.6873956469633384 1106 48-49 0.6544765752456954 1106 50-51 0.7137649613085166 1106 52-53 0.7923208185520956 1106 54-55 0.9835596563862943 1106 56-57 1.726255161416944 1106 58-59 -5.808233643435511 1106 60-61 -7.410072362140422 1106 62-63 -1.1352289226301693 1106 64-65 0.8859627070959171 1106 66-67 -0.7276489812789535 1106 68-69 -3.4849076892562145 1106 70-71 -4.260586907547202 1106 72-73 -5.18203018509068 1106 74-75 -5.4888841617097714 1106 76-77 -7.068503250719996 1106 78-79 -10.22109427172806 1106 80-81 -6.364599828408091 1106 82-83 -2.540500784358997 1106 84-85 -1.6959317887950007 1106 86-87 -1.5665006765864433 1106 88-89 -1.813235706650449 1106 90-91 -6.538463546806346 1106 92-93 -9.829792537423511 1106 94-95 -9.860462664032834 1106 96-97 -9.92875707566356 1106 98-99 -9.43830410368604 1106 100-101 -9.443801304739484 1107 1 -0.0031865301537266078 1107 2 0.0756981320625556 1107 3 0.08594361991568178 1107 4 0.13048376529247463 1107 5 0.07477371357789053 1107 6 0.09045161236005583 1107 7 -0.17557370686349572 1107 8 -0.1333012574855985 1107 9 0.017194713279138796 1107 10-11 0.12407225920506448 1107 12-13 -0.043559749240223766 1107 14-15 -0.17555648884106034 1107 16-17 0.08897198075466406 1107 18-19 0.018749196784881406 1107 20-21 0.060456775555785214 1107 22-23 0.1501415024361208 1107 24-25 0.39505827312896713 1107 26-27 0.576518475025857 1107 28-29 0.608017290845531 1107 30-31 0.4072137387999817 1107 32-33 0.04165086183767386 1107 34-35 -0.08878582727803064 1107 36-37 0.07704340205531679 1107 38-39 0.17849108778254674 1107 40-41 -0.0635664754125358 1107 42-43 -0.005219798997266878 1107 44-45 -0.10455099415390379 1107 46-47 -0.04057767914240884 1107 48-49 0.32141598111742553 1107 50-51 0.1350895022117271 1107 52-53 0.07627173572290147 1107 54-55 0.15145279716574933 1107 56-57 0.3719567063140765 1107 58-59 -6.358726619548669 1107 60-61 -7.935352729520137 1107 62-63 -2.5551330888919885 1107 64-65 -0.7438551883460995 1107 66-67 -2.214577954876095 1107 68-69 -3.412388039248798 1107 70-71 -4.372797782682079 1107 72-73 -5.7711404757113485 1107 74-75 -5.849681054528377 1107 76-77 -8.540665953292777 1107 78-79 -11.61426062048309 1107 80-81 -8.235508101099565 1107 82-83 -4.8110290972454095 1107 84-85 -4.095553107454961 1107 86-87 -3.8105174602752747 1107 88-89 -3.7902527409184295 1107 90-91 -7.461586887997978 1107 92-93 -9.663869511245663 1107 94-95 -9.728564705997169 1107 96-97 -10.699550030809894 1107 98-99 -10.272083195157656 1107 100-101 -9.780491461070186 1108 1 0.09886127941000922 1108 2 0.09005376012128607 1108 3 0.059158963450091306 1108 4 -0.033393858264169296 1108 5 -0.11993497252488794 1108 6 0.1596287519884072 1108 7 0.07640438421709916 1108 8 -0.03149039962928413 1108 9 0.08806332355737112 1108 10-11 0.13690775092542395 1108 12-13 -0.1387414274051295 1108 14-15 0.03316971346102093 1108 16-17 0.1054583514747236 1108 18-19 0.20205868305535546 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80-81 2.226845211535281 2312 82-83 1.2134986089062494 2312 84-85 0.946008751198935 2312 86-87 0.8781887089260785 2312 88-89 1.0585983176863536 2312 90-91 2.1640476609328907 2312 92-93 4.055108194028943 2312 94-95 7.303571447943439 2312 96-97 7.704419517857858 2312 98-99 7.691550617047433 2312 100-101 6.898400468274305 2313 1 -0.34973005836856785 2313 2 -0.35940860547011155 2313 3 -0.43496009704128724 2313 4 -0.356611254792071 2313 5 -0.410972396685402 2313 6 -0.4916898039075477 2313 7 -0.3861053773014902 2313 8 -0.2328960583065225 2313 9 -0.32969963673577496 2313 10-11 -0.5543399213278306 2313 12-13 -0.5838726822858789 2313 14-15 -0.7129497074556568 2313 16-17 -0.7867985116237293 2313 18-19 -0.8344283716692189 2313 20-21 -0.6026543121672105 2313 22-23 -0.8756368834327759 2313 24-25 -0.7258796489535086 2313 26-27 -0.7017928442395203 2313 28-29 -0.658264348895564 2313 30-31 -0.9155317926073394 2313 32-33 -0.5831004932115249 2313 34-35 -0.6784883303915734 2313 36-37 -0.7632791785898476 2313 38-39 -1.1714326084759463 2313 40-41 -0.8973883281024584 2313 42-43 -0.678502964813184 2313 44-45 -0.8610911895616482 2313 46-47 -0.919042482945315 2313 48-49 -0.8145765814604111 2313 50-51 -0.8154998784123855 2313 52-53 -0.7915535199384856 2313 54-55 -0.5344704781065737 2313 56-57 0.5726855386037144 2313 58-59 3.266413270644108 2313 60-61 3.24818934278424 2313 62-63 1.0153373631418994 2313 64-65 0.7740437398932976 2313 66-67 1.588731171753036 2313 68-69 0.4587384495512161 2313 70-71 2.297812998071839 2313 72-73 7.170481022648559 2313 74-75 8.470543822245467 2313 76-77 7.489571377654968 2313 78-79 5.498376857809813 2313 80-81 3.0128667169116277 2313 82-83 1.7005953830997953 2313 84-85 1.4363313318440944 2313 86-87 1.237866128280917 2313 88-89 1.0064477800519462 2313 90-91 2.0710369082447215 2313 92-93 3.493280237039695 2313 94-95 6.564861770524081 2313 96-97 7.147967904954633 2313 98-99 7.7200452407033495 2313 100-101 7.141948855371078 2314 1 -0.37547325887457816 2314 2 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2.4508717647266245 2314 62-63 0.3195378116497487 2314 64-65 -0.1205281651150365 2314 66-67 1.0626515903603568 2314 68-69 -0.1362445876519338 2314 70-71 2.638952093005999 2314 72-73 7.12766002328107 2314 74-75 7.899673832020639 2314 76-77 6.762402152193523 2314 78-79 4.6610558296918185 2314 80-81 1.9921031035483914 2314 82-83 0.8595914155303674 2314 84-85 0.36203428164571605 2314 86-87 0.22769995037280566 2314 88-89 0.30054817680888846 2314 90-91 1.3399432391055122 2314 92-93 2.892363095989726 2314 94-95 6.318340581403271 2314 96-97 6.864134312866774 2314 98-99 6.894408417058937 2314 100-101 6.083499082500049 2315 1 -0.6955669600718721 2315 2 -0.5663621690822538 2315 3 -0.8006633038164352 2315 4 -0.5037898636035649 2315 5 -0.6168054921274049 2315 6 -0.6529040012622005 2315 7 -0.905997850419773 2315 8 -1.0775006829140068 2315 9 -1.3160351586693508 2315 10-11 -0.9937573254576293 2315 12-13 -0.7118213453365954 2315 14-15 -0.8262335593920938 2315 16-17 -1.0148542257735045 2315 18-19 -0.8491074562633756 2315 20-21 -0.9894013861038289 2315 22-23 -0.9860684183076458 2315 24-25 -0.8621509402276573 2315 26-27 -0.9456730897433729 2315 28-29 -0.7997787651102399 2315 30-31 -0.7431890387772881 2315 32-33 -0.6083395739994231 2315 34-35 -0.850420721675242 2315 36-37 -1.0352353590066272 2315 38-39 -1.1817701762749735 2315 40-41 -0.8536484384840364 2315 42-43 -0.4968836385214388 2315 44-45 -0.6035999915409036 2315 46-47 -0.5173665385167325 2315 48-49 -0.5987449133663958 2315 50-51 -0.47297561349659745 2315 52-53 -0.39860699790974863 2315 54-55 -0.3252819254374337 2315 56-57 0.7477462008931823 2315 58-59 3.6082833343989833 2315 60-61 3.557877467248982 2315 62-63 1.4600026500388452 2315 64-65 1.2895399812102646 2315 66-67 2.300676884951244 2315 68-69 6.072463589907102 2315 70-71 5.015491892352928 2315 72-73 8.326892194025472 2315 74-75 9.13854149091042 2315 76-77 7.795762499550484 2315 78-79 5.776313626293966 2315 80-81 3.3150267701992853 2315 82-83 1.7746676069079612 2315 84-85 1.411256396074755 2315 86-87 1.368428027605308 2315 88-89 1.6136202036888285 2315 90-91 2.5395960004514 2315 92-93 4.286266011138089 2315 94-95 7.417058696968082 2315 96-97 8.118431317267891 2315 98-99 8.465560989109477 2315 100-101 7.394474071852189 2316 1 -0.36935827050634984 2316 2 -0.3720931325340828 2316 3 -0.6415575091061676 2316 4 -0.38639073356007003 2316 5 -0.6733926627688689 2316 6 -0.47774775907430467 2316 7 -0.5492181894028221 2316 8 -0.45786416473990954 2316 9 -0.5096704769034446 2316 10-11 -0.5176167574860244 2316 12-13 -0.4326971765450409 2316 14-15 -0.8825232449078229 2316 16-17 -1.0487631553270305 2316 18-19 -0.8811752278747917 2316 20-21 -0.7407625680447367 2316 22-23 -0.90798607242494 2316 24-25 -2.2014127271383046 2316 26-27 -1.580369479923867 2316 28-29 -1.2058222129378393 2316 30-31 -0.8783347814901532 2316 32-33 -0.6267217798891309 2316 34-35 -0.49431665187872653 2316 36-37 -0.5246515131989256 2316 38-39 -0.7660015897042953 2316 40-41 -0.5004592229448122 2316 42-43 -0.669299392733727 2316 44-45 -0.6843097060551742 2316 46-47 -0.42970404664132644 2316 48-49 -0.7426064702885498 2316 50-51 -0.6815195612992078 2316 52-53 -0.5976132975947657 2316 54-55 -0.4274174436615219 2316 56-57 0.4981730545505023 2316 58-59 3.6072552608026633 2316 60-61 3.634548008721911 2316 62-63 1.3731376182904356 2316 64-65 1.029210497684442 2316 66-67 5.538968095390722 2316 68-69 8.121204277872707 2316 70-71 5.591953694262832 2316 72-73 8.322841146577847 2316 74-75 8.855708757547088 2316 76-77 7.842824521449391 2316 78-79 5.400700531948683 2316 80-81 2.8238563287213587 2316 82-83 1.6514975154125366 2316 84-85 1.3591671255382813 2316 86-87 1.4181467916671018 2316 88-89 1.3659338380071127 2316 90-91 2.346351287687039 2316 92-93 3.892396329622162 2316 94-95 7.424156467079575 2316 96-97 7.954702003733566 2316 98-99 8.028784077955038 2316 100-101 7.176813079901786 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 10.0 4 28.0 5 96.0 6 257.0 7 663.0 8 1221.0 9 2169.0 10 3977.0 11 5880.0 12 7792.0 13 9837.0 14 12231.0 15 16184.0 16 20319.0 17 23542.0 18 20904.0 19 17426.0 20 17105.0 21 16892.0 22 15948.0 23 16200.0 24 16299.0 25 16104.0 26 18916.0 27 25707.0 28 33686.0 29 41949.0 30 49226.0 31 54201.0 32 57884.0 33 60300.0 34 61691.0 35 58438.0 36 47447.0 37 29152.0 38 9203.0 39 394.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.56394613514877 22.271209982181915 15.749360652704523 18.415483229964792 2 15.275972257065604 24.55482827914237 39.87375292367652 20.295446540115503 3 17.89881000648719 32.52627817872202 31.917217401881288 17.657694412909503 4 11.085785670756042 20.59608681825611 38.485406338173604 29.832721172814246 5 10.385765705686136 42.16633301341501 32.92694832156989 14.520952959328959 6 24.083311266836528 40.90862443160294 18.51127798591376 16.496786315646773 7 23.420682874666944 38.961535878856836 21.090967602159434 16.52681364431679 8 25.698452135551214 33.55953076280818 20.619905965049202 20.122111136591403 9 28.215933235438328 17.03284381608071 21.131764225922073 33.61945872255889 10-11 23.109513595284824 29.638810512352382 26.341695796554077 20.909980095808717 12-13 26.6998065327811 28.644487831330036 27.028966869847366 17.6267387660415 14-15 23.52126211310442 28.574921811601595 28.626487828456206 19.27732824683778 16-17 18.05903094081829 32.26708865410418 29.170029431849997 20.503850973227532 18-19 17.73273750774451 29.06366523960779 35.26414948515652 17.939447767491185 20-21 19.764633888311007 27.7316950490383 34.85317649861076 17.65049456403993 22-23 19.194368038759325 28.473952876098625 34.30565286634291 18.026026218799135 24-25 18.41707832825065 29.22311572177716 35.28559537097687 17.074210578995313 26-27 18.547152300680054 28.916889180294636 35.62422595348277 16.911732565542536 28-29 17.728967060367786 28.445560536298466 36.34065747495762 17.484814928376128 30-31 17.6118487657958 30.08839185365674 35.61345289547942 16.686306485068037 32-33 17.12273748919899 30.19810510365169 35.019600191567484 17.659557215581838 34-35 17.83999548951153 29.210037357233855 35.15609014135187 17.793877011902747 36-37 17.986505906833983 29.100123433947626 34.727266736452044 18.18610392276634 38-39 17.876946297306596 28.972448800492213 35.14211542470515 18.008489477496042 40-41 17.166579433282937 29.161634377641555 35.736201178347706 17.935585010727806 42-43 17.156527899405248 29.43279848225816 34.79774214356956 18.612931474767038 44-45 17.030358216691603 29.753130776244944 34.10260163498029 19.113909372083164 46-47 17.544820174337993 29.437849949685823 32.84495628876537 20.17237358721082 48-49 17.460539747938512 29.469674743220835 32.418714320385114 20.651071188455536 50-51 17.666232196932015 29.781094420179333 32.1063206792175 20.446352703671153 52-53 17.375789010810763 30.076186034841296 32.32457379793466 20.223451156413276 54-55 17.083901445073522 29.589096816377836 32.026106107222326 21.300895631326313 56-57 18.122565834307316 30.032766455880132 30.620265710322702 21.224401999489846 58-59 16.750823313189805 29.158537839199607 32.46622292581593 21.624415921794665 60-61 18.427016995258615 29.743418627413593 30.517548208315855 21.312016169011937 62-63 18.52117917969282 29.659467044227156 30.766048696434396 21.053305079645625 64-65 17.85265198761082 29.415548903892493 31.403223166397453 21.32857594209923 66-67 19.041725601131542 29.040246881831038 30.304616175903305 21.613411341134114 68-69 20.44802534442804 28.64748234266475 29.53910036406541 21.365391948841804 70-71 19.553026634696476 28.749772312854134 29.96246160108953 21.734739451359868 72-73 19.119378719341775 28.619900953197895 29.724698464044668 22.536021863415662 74-75 19.24413561823517 28.88246252198197 29.55298114524555 22.320420714537303 76-77 17.378063047152995 29.038331238257264 31.121206507406697 22.462399207183044 78-79 17.644816879183526 28.77847344175511 30.502150619734795 23.074559059326564 80-81 17.607145255353306 29.70502192507339 29.644650370781434 23.04318244879187 82-83 18.02554226636935 29.26489965538212 28.840398844516525 23.86915923373201 84-85 17.840921040795358 28.489916772353574 28.898769893105243 24.77039229374583 86-87 18.216897359340187 28.644345574647435 29.064552770434343 24.074204295578035 88-89 18.728881095579393 28.053950367486358 29.021983298723775 24.195185238210474 90-91 18.34178703776609 28.008600173222625 29.366188060846355 24.28342472816493 92-93 18.30066935712649 28.32408130781987 29.302509184859215 24.07274015019442 94-95 17.974126473107482 27.916390034300274 29.632944699638248 24.476538792954 96-97 17.79127478695352 27.786357835925322 29.450739339973143 24.971628037148015 98-99 18.04359376500336 27.704573824536695 29.05175559325289 25.200076817207055 100-101 18.03153127649951 27.793593106199065 29.69522819557458 24.479647421726845 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2455.0 1 1768.0 2 1086.0 3 1169.0 4 1218.0 5 1384.5 6 1986.5 7 3016.5 8 3942.5 9 4374.5 10 4396.5 11 4298.0 12 4439.0 13 4502.5 14 4375.0 15 4212.0 16 3959.5 17 3727.5 18 3548.5 19 3454.5 20 3550.5 21 3686.5 22 3937.5 23 4276.0 24 4619.0 25 5221.5 26 6176.0 27 7264.5 28 8350.0 29 9624.5 30 11309.0 31 13526.0 32 16565.0 33 19841.0 34 22998.0 35 26071.5 36 29222.5 37 31225.5 38 31257.0 39 30662.5 40 29779.5 41 28998.0 42 28522.5 43 28406.0 44 28386.5 45 28228.5 46 28096.0 47 27993.5 48 28517.5 49 26545.5 50 21974.0 51 17710.0 52 14094.0 53 11925.0 54 10828.0 55 10150.5 56 9498.5 57 9117.0 58 8537.0 59 7689.0 60 6873.0 61 5856.0 62 4815.0 63 3618.0 64 2588.0 65 2023.5 66 1580.0 67 1165.5 68 857.5 69 659.0 70 533.5 71 485.0 72 439.5 73 421.0 74 341.0 75 204.0 76 134.5 77 93.0 78 58.0 79 30.0 80 12.0 81 10.0 82 8.0 83 4.5 84 2.5 85 1.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024706029918368743 2 0.004434415626373877 3 0.004181020447723941 4 0.0 5 0.06816330305683273 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.900463839874519E-4 16-17 0.0 18-19 0.0015203710718996151 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.434415626373877E-4 28-29 0.0024072541971743903 30-31 0.008615436074097817 32-33 3.800927679749038E-4 34-35 0.0012036270985871951 36-37 0.024706029918368743 38-39 0.023629100409106516 40-41 0.026606493758243265 42-43 0.027113284115543132 44-45 0.027810120856830454 46-47 0.030280723848667333 48-49 0.0288237015714302 50-51 0.024896076302356192 52-53 0.020271614291994866 54-55 0.014823617951021247 56-57 0.16217291433595893 58-59 0.8002853229711597 60-61 0.32827345394099183 62-63 4.434415626373877E-4 64-65 0.14874296986751234 66-67 1.4672847819724535 68-69 2.818831316096549 70-71 2.272638008516612 72-73 1.9261201017128244 74-75 1.620778911439652 76-77 1.1754368849623897 78-79 0.43850035665371395 80-81 2.5339517864993584E-4 82-83 1.2669758932496792E-4 84-85 0.0 86-87 0.003484183706436618 88-89 0.0 90-91 0.23502402819781548 92-93 0.18187438947599144 94-95 1.339193519164911 96-97 1.310433166388143 98-99 1.039680418000687 100-101 0.41803869597773163 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 789281.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.10551618958959 #Duplication Level Percentage of deduplicated Percentage of total 1 77.30949790785058 25.593708345973905 2 9.467016613173524 6.268209435090595 3 3.7379353442797107 3.7123883716707335 4 2.07826114901685 2.752076324598896 5 1.2911972374361969 2.1372875523948687 6 0.9015734382958622 1.7908232434564575 7 0.6533589949680467 1.514085074987006 8 0.5336678160384285 1.4133878818978494 9 0.423369376145208 1.2614275562536466 >10 2.9552263490245254 19.544616489495606 >50 0.40455611624689475 9.340188971192141 >100 0.22789739575028822 13.44481362648226 >500 0.00917707633893778 2.0688321055428407 >1k 0.005735672711836113 3.9441107454199527 >5k 7.647563615781484E-4 1.991866437050517 >10k+ 7.647563615781484E-4 3.2221778384927777 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14097 1.7860559167140726 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11357 1.4389045219636605 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8774 1.1116446487372684 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6961 0.8819419192911018 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3797 0.48107074666690314 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2923 0.3703370535968812 No Hit CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 2753 0.34879846341163667 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2603 0.3297938250128915 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2338 0.296218963841775 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2251 0.28519627357050276 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2076 0.2630241954386334 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1735 0.2198203174788193 No Hit ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT 1727 0.21880673676421958 TruSeq Adapter, Index 3 (95% over 21bp) GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1716 0.21741306328164492 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1511 0.19144005747002651 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1480 0.1875124322009525 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1467 0.18586536353972793 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1452 0.1839648996998534 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1328 0.1682543986235574 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 966 0.122389871287919 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 943 0.11947582673344476 No Hit ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT 899 0.11390113280314615 RNA PCR Primer, Index 3 (95% over 23bp) GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 829 0.10503230155039842 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 820 0.10389202324647369 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 817 0.10351193047849877 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 811 0.10275174494254898 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.2669758932496792E-4 0.0 3 0.0 0.0 0.0 1.2669758932496792E-4 0.0 4 0.0 0.0 0.0 1.2669758932496792E-4 0.0 5 0.0 0.0 0.0 1.2669758932496792E-4 0.0 6 0.0 0.0 0.0 1.2669758932496792E-4 0.0 7 0.0 0.0 0.0 1.2669758932496792E-4 0.0 8 0.0 0.0 0.0 1.2669758932496792E-4 0.0 9 0.0 0.0 0.0 1.2669758932496792E-4 0.0 10-11 0.0 0.0 0.0 1.900463839874519E-4 0.0 12-13 0.0 0.0 0.0 5.067903572998717E-4 0.0 14-15 0.0 0.0 0.0 5.701391519623556E-4 0.0 16-17 0.0 0.0 0.0 0.0012669758932496791 0.0 18-19 0.0 0.0 0.0 0.001837115045212035 0.0 20-21 0.0 0.0 0.0 0.004307718037048909 0.0 22-23 0.0 0.0 1.2669758932496792E-4 0.015520454692308568 0.0 24-25 0.0 0.0 1.2669758932496792E-4 0.03408165152841637 0.0 26-27 0.0 0.0 1.2669758932496792E-4 0.06442572417174619 0.0 28-29 0.0 0.0 1.2669758932496792E-4 0.11865229240283245 0.0 30-31 0.0 0.0 1.2669758932496792E-4 0.292164640983376 0.0 32-33 0.0 0.0 1.2669758932496792E-4 0.509451006675696 0.0 34-35 0.0 0.0 1.2669758932496792E-4 0.8178962878873304 0.0 36-37 0.0 0.0 1.2669758932496792E-4 1.2401793531074485 0.0 38-39 0.0 0.0 1.2669758932496792E-4 1.8027799985049684 0.0 40-41 0.0 0.0 1.2669758932496792E-4 2.4286027409756477 0.0 42-43 0.0 0.0 1.2669758932496792E-4 3.0107148151292127 0.0 44-45 0.0 0.0 1.2669758932496792E-4 3.5963110729892143 0.0 46-47 0.0 0.0 1.2669758932496792E-4 4.2701522018140565 0.0 48-49 0.0 1.2669758932496792E-4 1.2669758932496792E-4 4.958246809437957 0.0 50-51 0.0 1.2669758932496792E-4 1.2669758932496792E-4 5.632658077414761 0.0 52-53 0.0 1.2669758932496792E-4 1.2669758932496792E-4 6.213439826880414 0.0 54-55 0.0 1.2669758932496792E-4 1.2669758932496792E-4 6.788330138442456 0.0 56-57 0.0 1.2669758932496792E-4 1.2669758932496792E-4 7.256921172560849 1.2669758932496792E-4 58-59 0.0 1.2669758932496792E-4 1.2669758932496792E-4 7.595520480031826 1.2669758932496792E-4 60-61 0.0 1.2669758932496792E-4 1.2669758932496792E-4 7.871151085608294 1.2669758932496792E-4 62-63 0.0 1.2669758932496792E-4 1.2669758932496792E-4 8.087043777818039 1.2669758932496792E-4 64-65 0.0 1.2669758932496792E-4 1.2669758932496792E-4 8.283741785245052 1.2669758932496792E-4 66-67 0.0 1.2669758932496792E-4 1.2669758932496792E-4 8.444394328509112 1.2669758932496792E-4 68-69 0.0 1.2669758932496792E-4 1.2669758932496792E-4 8.623861463787929 1.2669758932496792E-4 70-71 0.0 1.2669758932496792E-4 1.2669758932496792E-4 8.97791787715655 1.2669758932496792E-4 72-73 0.0 1.2669758932496792E-4 1.2669758932496792E-4 9.401594615859244 1.2669758932496792E-4 74-75 0.0 1.2669758932496792E-4 1.2669758932496792E-4 9.867081559039176 1.2669758932496792E-4 76-77 0.0 1.2669758932496792E-4 1.2669758932496792E-4 10.503103457450514 1.2669758932496792E-4 78-79 0.0 1.2669758932496792E-4 1.2669758932496792E-4 11.33797722230739 1.2669758932496792E-4 80-81 0.0 1.2669758932496792E-4 1.2669758932496792E-4 12.232588900530988 1.2669758932496792E-4 82-83 0.0 1.2669758932496792E-4 1.2669758932496792E-4 13.069426478022402 1.2669758932496792E-4 84-85 0.0 1.2669758932496792E-4 1.2669758932496792E-4 13.801865241910043 1.2669758932496792E-4 86-87 0.0 1.2669758932496792E-4 1.2669758932496792E-4 14.454737919701602 1.2669758932496792E-4 88-89 0.0 1.2669758932496792E-4 1.2669758932496792E-4 15.033049066175419 1.2669758932496792E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTTACG 15 0.0093963295 48.64771 64-65 CGTCACT 180 0.0 39.306953 1 GGTACGC 25 0.001438263 38.911804 68-69 GGTATCA 9835 0.0 30.07072 1 GTATCAA 14120 0.0 27.693031 1 CATATAG 265 0.0 26.693985 3 GTACATG 9430 0.0 26.110155 1 CCTTTAG 235 7.2759576E-12 26.093128 1 TACATGG 9320 0.0 25.959543 2 CTTTAGG 255 0.0 25.893063 2 TAGGCAT 220 8.367351E-11 25.721664 5 ATGGGAC 1065 0.0 25.681414 5 CGGGAGT 600 0.0 25.15007 4 ACATGGG 9305 0.0 24.935425 3 GTCGGGA 595 0.0 24.571987 2 ATGGGAT 2240 0.0 23.999231 5 CATGGGG 1890 0.0 23.95245 4 GGGACTC 415 0.0 23.842615 7 GAGTACT 9425 0.0 23.650755 12-13 GTCACTA 320 0.0 23.58118 2 >>END_MODULE