FastQCFastQC Report
Fri 27 May 2016
SRR939808_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939808_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872659
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140641.6116260761649168No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100551.1522255543115927No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79880.9153632747728494No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66060.7569967192225141No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA39180.4489726227541342No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA29700.3403391244460895No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24820.28441808312296096No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23280.26677086926279336No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18900.21657944282932967No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17760.20351592088089393No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT16230.1859832993185196No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15450.1770451000906425No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG15210.1742948849436034No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT13700.1569914479768157No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA13690.15687685567902238No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13530.15504337891432965No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC11360.1301768502931844No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG10450.11974895119399444No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9100.10427899099189947No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG2200.040.644099
GTACAGG2400.035.2921261
CGTCACT1950.033.7839161
CTTTAGG3000.029.8022422
TAGGCAT3050.029.3170475
CCTTTAG3050.029.313681
GTACATG106700.027.3869571
TACATGG107250.026.8955082
GTATCAA172000.026.8110711
CATGGGG25300.026.4109734
GGCATTC3450.025.9179677
GTCGGGA6600.025.6670022
ACATGGG110300.025.5118663
CCCTATA1301.07740725E-525.3379382
TTAGGCA4100.025.2496534
CATGGGA77050.024.5510444
GTAATAC1351.443498E-524.3994963
TCGGGAG7150.024.3507463
GAGTACT93800.024.2812812-13
CATGGAT2151.8517312E-924.075254
Produced by FastQC (version 0.11.5)