##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939808_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 872659 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.00945844825986 31.0 30.0 33.0 26.0 34.0 2 30.000104278990992 31.0 30.0 33.0 26.0 34.0 3 29.84955291815016 31.0 30.0 34.0 25.0 34.0 4 33.53768081232188 35.0 33.0 37.0 28.0 37.0 5 33.46124889561673 35.0 33.0 37.0 28.0 37.0 6 33.53484006925959 35.0 33.0 37.0 28.0 37.0 7 33.549209943402865 35.0 33.0 37.0 28.0 37.0 8 33.42158735542749 35.0 33.0 37.0 27.0 37.0 9 34.60057364904275 37.0 34.0 39.0 27.0 39.0 10-11 34.434448621970326 37.0 34.0 39.0 27.0 39.0 12-13 34.352292819990396 37.0 33.5 39.0 27.0 39.0 14-15 34.69629947092736 38.0 33.0 40.0 25.0 41.0 16-17 34.60048770481941 38.0 33.0 40.0 25.0 41.0 18-19 34.87789560412486 38.0 33.5 40.0 25.5 41.0 20-21 35.10088304824679 38.0 34.0 40.0 26.0 41.0 22-23 34.990393154714496 38.0 34.0 40.0 25.5 41.0 24-25 34.727781985861604 38.0 33.0 40.0 25.0 41.0 26-27 34.41657279647606 38.0 33.0 40.0 24.0 41.0 28-29 34.19643812760769 38.0 33.0 40.0 23.5 41.0 30-31 33.8107078480827 37.5 33.0 40.0 22.0 41.0 32-33 33.2929792736911 36.5 32.0 40.0 19.5 41.0 34-35 32.87036803608282 36.0 31.5 40.0 17.5 41.0 36-37 32.45614896540344 36.0 31.0 39.5 15.5 41.0 38-39 31.9854960528683 35.0 31.0 39.0 11.5 41.0 40-41 31.507682267644064 35.0 30.0 39.0 8.5 41.0 42-43 30.93772768057168 35.0 30.0 39.0 4.0 40.5 44-45 30.21968489409953 35.0 27.5 39.0 2.0 40.0 46-47 29.47925077263857 35.0 25.5 39.0 2.0 40.0 48-49 28.89666868731085 35.0 24.0 39.0 2.0 40.0 50-51 28.065613257870485 34.0 23.0 38.0 2.0 39.5 52-53 28.090885443225822 34.0 23.0 38.0 2.0 40.0 54-55 28.094222943899048 34.5 23.0 38.0 2.0 40.0 56-57 27.853398062702613 34.0 22.5 38.0 2.0 40.0 58-59 27.325536091417153 34.0 20.0 38.0 2.0 40.0 60-61 26.785343415927642 33.5 18.0 38.0 2.0 40.0 62-63 26.29447126540837 33.0 14.5 37.5 2.0 40.0 64-65 25.7233060107098 33.0 10.0 37.0 2.0 39.0 66-67 25.1456147246519 32.0 7.5 36.0 2.0 39.0 68-69 24.56034487697944 31.0 2.0 36.0 2.0 39.0 70-71 24.012605152757263 31.0 2.0 35.0 2.0 38.5 72-73 23.48886162865449 31.0 2.0 35.0 2.0 38.0 74-75 22.849732255096207 30.0 2.0 35.0 2.0 37.0 76-77 22.13658714343174 29.5 2.0 34.5 2.0 36.5 78-79 21.447769976588795 29.0 2.0 34.0 2.0 36.0 80-81 20.748066541455483 28.5 2.0 34.0 2.0 35.5 82-83 19.98507607209689 26.5 2.0 34.0 2.0 35.0 84-85 19.25182173105417 26.0 2.0 33.0 2.0 35.0 86-87 18.47666098670844 24.5 2.0 33.0 2.0 35.0 88-89 17.654238368022334 22.0 2.0 32.0 2.0 34.5 90-91 16.777205643899848 19.0 2.0 32.0 2.0 34.0 92-93 15.874008633383717 5.0 2.0 31.0 2.0 34.0 94-95 14.677858705404976 2.0 2.0 31.0 2.0 34.0 96-97 12.517749201005204 2.0 2.0 29.5 2.0 34.0 98-99 11.36216551940678 2.0 2.0 28.0 2.0 33.5 100-101 10.273366228962287 2.0 2.0 24.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0591240107821136 1101 2 0.9373054359185247 1101 3 0.9695517975555994 1101 4 1.204202951739596 1101 5 1.0508782998953095 1101 6 1.0687833661102815 1101 7 0.8512855887568378 1101 8 0.9771775098671327 1101 9 1.180600276365034 1101 10-11 1.166342480238022 1101 12-13 0.9790531722694809 1101 14-15 1.2630496457501366 1101 16-17 1.3979368235329162 1101 18-19 1.2412308047791214 1101 20-21 1.100667955619926 1101 22-23 1.0237235189805673 1101 24-25 1.1656685976562642 1101 26-27 1.0575060435087167 1101 28-29 1.4011052613113222 1101 30-31 1.1820978073407176 1101 32-33 1.370129548576358 1101 34-35 1.3404294457502957 1101 36-37 1.1829009724333233 1101 38-39 1.0227558767845224 1101 40-41 1.2474030796802182 1101 42-43 1.2912714799875715 1101 44-45 1.2394572156879526 1101 46-47 1.2829754725579399 1101 48-49 0.9886361976145892 1101 50-51 1.0995349323698633 1101 52-53 1.2180828228324039 1101 54-55 0.9241079153851182 1101 56-57 0.6220368360558162 1101 58-59 0.4259676815499738 1101 60-61 0.45356735650701197 1101 62-63 0.5940388634195486 1101 64-65 0.8539578932363057 1101 66-67 0.5503755417413458 1101 68-69 0.4456126465653014 1101 70-71 0.10535123368078914 1101 72-73 0.2875360312145041 1101 74-75 0.5471520366561364 1101 76-77 0.24087264651498685 1101 78-79 0.27832475012114344 1101 80-81 -0.23893398341979832 1101 82-83 -0.06384701004123627 1101 84-85 -0.0702253266380346 1101 86-87 -0.4583568221444274 1101 88-89 -0.6937535268432136 1101 90-91 -0.4775657341128152 1101 92-93 -0.4308685496005076 1101 94-95 -0.5673942608674025 1101 96-97 0.9747200786041468 1101 98-99 0.8906208253718155 1101 100-101 0.2691182718665477 1102 1 0.7764039130628433 1102 2 0.6650505794437045 1102 3 0.6688094666049587 1102 4 0.8257321220432132 1102 5 0.8134110324438453 1102 6 0.8232112706242987 1102 7 0.8347744918003457 1102 8 0.896595807591126 1102 9 0.9256455056042725 1102 10-11 0.7903303596931295 1102 12-13 0.5810253820408846 1102 14-15 0.8946292484248417 1102 16-17 0.937937373990188 1102 18-19 0.9584432105133089 1102 20-21 0.7353936837307273 1102 22-23 0.6008812459590018 1102 24-25 0.7058172473321562 1102 26-27 1.112990170680078 1102 28-29 1.1389375343591013 1102 30-31 1.0481984204452672 1102 32-33 1.0506756021908927 1102 34-35 0.9991714936086282 1102 36-37 0.9561119207663609 1102 38-39 0.8327701362491098 1102 40-41 1.051418665484718 1102 42-43 0.35387419924649777 1102 44-45 0.22227180857237627 1102 46-47 0.5411746646963635 1102 48-49 0.4909953002119636 1102 50-51 0.4711735388217484 1102 52-53 0.4710900233377764 1102 54-55 0.6236879951227827 1102 56-57 0.39488081287370136 1102 58-59 0.31466259978085276 1102 60-61 0.436096891160922 1102 62-63 0.41215008724333657 1102 64-65 0.3543471451648692 1102 66-67 0.2720060223953915 1102 68-69 0.007301868874023398 1102 70-71 -0.10705843234891788 1102 72-73 -0.12413785692733725 1102 74-75 -0.35264180398910483 1102 76-77 -0.34597771560726187 1102 78-79 -0.4267861145948082 1102 80-81 -0.660006274187058 1102 82-83 -1.0214277241153802 1102 84-85 -1.020113400325915 1102 86-87 -1.1231407938771376 1102 88-89 -1.2692336697524063 1102 90-91 -0.8840948126735544 1102 92-93 -0.9817530820132667 1102 94-95 -0.9073874718943742 1102 96-97 0.22010016935096033 1102 98-99 0.6648940251799438 1102 100-101 0.504755268886214 1103 1 0.4996117114829879 1103 2 0.6566173741016854 1103 3 0.7749918348320861 1103 4 0.8399068366484457 1103 5 0.9530741179394582 1103 6 0.7382069396051492 1103 7 0.7392309165763606 1103 8 0.7905776302391203 1103 9 0.9297916484115518 1103 10-11 1.0164948867070507 1103 12-13 0.7276232695378084 1103 14-15 0.923509389086 1103 16-17 1.0041806350536788 1103 18-19 0.9091672017737054 1103 20-21 1.0752481097737316 1103 22-23 1.1471475196018446 1103 24-25 1.021125137544054 1103 26-27 1.2896162421224986 1103 28-29 1.3128395886843052 1103 30-31 1.2653330957481117 1103 32-33 1.4329171308467323 1103 34-35 1.119397353025036 1103 36-37 1.3116426968591313 1103 38-39 1.49160282962589 1103 40-41 1.6869685756322284 1103 42-43 1.4800594117250085 1103 44-45 1.412631735656113 1103 46-47 1.3089564071881838 1103 48-49 1.408683958113162 1103 50-51 1.5484001351578343 1103 52-53 1.4531557255446579 1103 54-55 1.4921258546943363 1103 56-57 1.1605539308196349 1103 58-59 1.0063431644940373 1103 60-61 1.3052845988235902 1103 62-63 0.7876552650736919 1103 64-65 0.7529211409502068 1103 66-67 0.7599354073906746 1103 68-69 0.2563432268747725 1103 70-71 0.32992459264171003 1103 72-73 0.20150694778894973 1103 74-75 0.09287203587954451 1103 76-77 -0.004761927426418566 1103 78-79 0.2752707962896288 1103 80-81 -0.16233080326952987 1103 82-83 -0.20577529163402986 1103 84-85 -0.10630003493011131 1103 86-87 0.053220245050322745 1103 88-89 0.09253377391202733 1103 90-91 0.07760152470637394 1103 92-93 -0.009599595866063737 1103 94-95 0.2734545557982919 1103 96-97 1.4307047020533865 1103 98-99 1.3618946328154635 1103 100-101 1.0028593167730655 1104 1 0.3577541547501468 1104 2 0.6695419670266531 1104 3 0.7203096078755813 1104 4 0.9212539706265659 1104 5 0.5845755350821733 1104 6 0.963799720032867 1104 7 0.8243688287574997 1104 8 0.6989365726924461 1104 9 0.821765781629253 1104 10-11 0.8526612485780518 1104 12-13 0.7084374824422994 1104 14-15 1.0389282788756518 1104 16-17 0.9896748753583537 1104 18-19 0.7411581773083498 1104 20-21 0.9482846915375234 1104 22-23 0.8277637724376987 1104 24-25 0.73222912628232 1104 26-27 1.0662060500069686 1104 28-29 1.1597265880495797 1104 30-31 1.2997511245685942 1104 32-33 1.4148776268152545 1104 34-35 1.3223585842738146 1104 36-37 1.1961886141683848 1104 38-39 1.22382821501958 1104 40-41 0.9631429815949133 1104 42-43 1.0232894916003232 1104 44-45 0.9708018304884156 1104 46-47 1.0981239065440285 1104 48-49 0.8908700657154576 1104 50-51 0.8899862037965605 1104 52-53 1.1433727274865504 1104 54-55 1.134628195650702 1104 56-57 0.9441798051432713 1104 58-59 0.8811437904249182 1104 60-61 1.0931681857343456 1104 62-63 0.7951061049899835 1104 64-65 0.9933854358667169 1104 66-67 1.0663166344917236 1104 68-69 0.9698906084600871 1104 70-71 0.7941195864515294 1104 72-73 0.6258258708861781 1104 74-75 0.37483253184807097 1104 76-77 0.374256819077857 1104 78-79 0.5536981763666731 1104 80-81 0.37402849111280645 1104 82-83 0.6168380326496994 1104 84-85 0.2726483257719856 1104 86-87 0.41589126055405146 1104 88-89 0.5223635282530061 1104 90-91 0.19544118333523386 1104 92-93 0.41807459328320995 1104 94-95 0.7690843516166197 1104 96-97 1.7834154981042403 1104 98-99 2.0047300494428537 1104 100-101 1.8988347571556616 1105 1 0.660755259656753 1105 2 0.9453122704605441 1105 3 0.8041436213899154 1105 4 0.6180222118161396 1105 5 0.9660250088564482 1105 6 0.8233872959719477 1105 7 0.9111108175645839 1105 8 0.9712892301106351 1105 9 0.889706997125721 1105 10-11 0.8071114428357617 1105 12-13 0.6607355269684163 1105 14-15 0.7145699865577981 1105 16-17 1.0981057715727687 1105 18-19 0.9722758690828144 1105 20-21 0.848167256458531 1105 22-23 0.8397329739411603 1105 24-25 0.8453320041237973 1105 26-27 0.8343567141290009 1105 28-29 0.6082064424151952 1105 30-31 0.9539221779996936 1105 32-33 1.0018285682180021 1105 34-35 1.3736214143972205 1105 36-37 1.179972156640325 1105 38-39 1.1719856499497538 1105 40-41 0.9614843816144969 1105 42-43 1.0210982795140566 1105 44-45 1.0109387099395057 1105 46-47 0.7863046300780425 1105 48-49 0.5399010093007348 1105 50-51 0.42171041613207905 1105 52-53 0.4857294647506727 1105 54-55 0.8604420877121157 1105 56-57 0.7749635447870951 1105 58-59 0.615783294494225 1105 60-61 0.8287507666523233 1105 62-63 0.5859017741529193 1105 64-65 0.30397455817901076 1105 66-67 0.5469429466458173 1105 68-69 0.0024611718136462457 1105 70-71 0.17725184166387464 1105 72-73 0.31733371497901786 1105 74-75 0.23212906968337066 1105 76-77 0.4328757726283783 1105 78-79 0.2745282080009801 1105 80-81 0.13295857941852773 1105 82-83 -0.17278018553649233 1105 84-85 -0.10071834077067265 1105 86-87 0.023238951472858815 1105 88-89 0.1649181814475007 1105 90-91 0.44400176656719026 1105 92-93 0.6239208016926536 1105 94-95 0.881660817725999 1105 96-97 2.2886473734816875 1105 98-99 2.0173261087333074 1105 100-101 1.0162995979362641 1106 1 0.9617586041383568 1106 2 1.043058052067785 1106 3 1.0416585014987518 1106 4 0.9777599439930782 1106 5 0.8943170557925626 1106 6 1.1766250187011948 1106 7 0.9414908035820062 1106 8 0.7273007940809322 1106 9 1.0970232868296321 1106 10-11 1.1249694629782283 1106 12-13 0.9061543429841379 1106 14-15 1.2760517619673521 1106 16-17 1.4510732904209078 1106 18-19 1.0611446029036458 1106 20-21 0.6786379402822078 1106 22-23 0.7155091015227484 1106 24-25 0.8267748550581686 1106 26-27 0.9896162421224943 1106 28-29 0.8066248994187646 1106 30-31 0.7178754686294724 1106 32-33 1.0589058314116997 1106 34-35 1.1815442456803993 1106 36-37 1.3393263126783452 1106 38-39 1.069003959569386 1106 40-41 1.1609798750672553 1106 42-43 0.8772345529679413 1106 44-45 1.4188464249216501 1106 46-47 1.29426714165146 1106 48-49 1.2922997773221994 1106 50-51 1.3314509826154612 1106 52-53 1.5096529006859 1106 54-55 1.3339337642988554 1106 56-57 1.475808168107772 1106 58-59 1.115947684268047 1106 60-61 1.5092394010834767 1106 62-63 1.5181637396499639 1106 64-65 1.7896443047920165 1106 66-67 1.4672800401590358 1106 68-69 1.5580381421290106 1106 70-71 1.8604330672179792 1106 72-73 1.520715987336974 1106 74-75 1.4431545217552468 1106 76-77 1.5257465471498541 1106 78-79 1.1747058245382114 1106 80-81 1.169872586560981 1106 82-83 1.229252956953541 1106 84-85 1.2338129594201703 1106 86-87 1.1532202450503206 1106 88-89 0.9789744518781269 1106 90-91 0.9081099992826474 1106 92-93 1.0163891046989075 1106 94-95 1.4655449512785168 1106 96-97 2.693416566460167 1106 98-99 2.851725141290041 1106 100-101 2.5028593167730655 1107 1 0.6757347688187458 1107 2 0.634448200870203 1107 3 0.6819679811493629 1107 4 0.4586176237526445 1107 5 0.7320055072313139 1107 6 0.8732247824291477 1107 7 0.7488642052261199 1107 8 0.5184338593128288 1107 9 0.7639984537809283 1107 10-11 0.7592680839085091 1107 12-13 1.0128403521289684 1107 14-15 0.8465042902618478 1107 16-17 0.923965052116678 1107 18-19 0.9521540231801993 1107 20-21 0.9213768596742611 1107 22-23 0.8821121094005164 1107 24-25 0.9673977318233256 1107 26-27 0.9997122811992014 1107 28-29 1.0915389906589468 1107 30-31 1.2000791645118554 1107 32-33 1.280056464020916 1107 34-35 1.1210659478184795 1107 36-37 0.9703532391218417 1107 38-39 1.298620598997438 1107 40-41 0.9456491248117018 1107 42-43 0.8706201599606125 1107 44-45 0.9946009958063264 1107 46-47 0.8428938869122469 1107 48-49 0.6637096358650254 1107 50-51 0.4848080655738798 1107 52-53 0.6296913408007292 1107 54-55 0.6458300004729658 1107 56-57 0.7420604405429039 1107 58-59 0.6854128892309852 1107 60-61 1.0700794522553494 1107 62-63 0.7137143575686871 1107 64-65 0.7033731795617122 1107 66-67 0.7137038240619304 1107 68-69 0.6458499324712399 1107 70-71 0.8737126578429972 1107 72-73 0.9753486944881793 1107 74-75 0.902524911848964 1107 76-77 0.8092791202503413 1107 78-79 0.6896521736454595 1107 80-81 0.5548264645442202 1107 82-83 0.7440866789727494 1107 84-85 0.6880533080744584 1107 86-87 0.5285775606081593 1107 88-89 0.722842090565532 1107 90-91 0.8227368302379539 1107 92-93 1.1242191357325684 1107 94-95 1.1062749461980559 1107 96-97 2.41200064809032 1107 98-99 2.4916379777175592 1107 100-101 1.8334051296774376 1108 1 0.5726233491494419 1108 2 0.5408846019819755 1108 3 0.670643681951482 1108 4 0.666817531025039 1108 5 0.8647969686040184 1108 6 0.8024082749929278 1108 7 0.6811189995557996 1108 8 0.6806987671977964 1108 9 0.8179798554091846 1108 10-11 0.6270928047047164 1108 12-13 0.266290011367019 1108 14-15 0.1843540907533523 1108 16-17 0.43713189214714276 1108 18-19 0.6325452568177354 1108 20-21 0.45294988987320295 1108 22-23 0.6447210223014324 1108 24-25 0.8182269953114698 1108 26-27 1.0622739895012074 1108 28-29 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3.4820523711592237 2312 1 -0.13973712235737068 2312 2 -0.5668579264922613 2312 3 -0.783091607149661 2312 4 -0.5045158781132528 2312 5 -2.0745732817418414 2312 6 -0.7503306527460012 2312 7 0.1631082163011186 2312 8 0.013513310378193921 2312 9 0.15461781094958127 2312 10-11 -0.09592181617576045 2312 12-13 -0.06550471829356042 2312 14-15 0.13648563129952862 2312 16-17 -0.5919025896312391 2312 18-19 -0.1400197459301893 2312 20-21 0.16879620480487745 2312 22-23 0.10434185539786967 2312 24-25 0.1380670468588434 2312 26-27 0.6275562681981199 2312 28-29 0.3525161785727562 2312 30-31 0.30414049832671 2312 32-33 -0.028666600864127645 2312 34-35 0.19529551324522743 2312 36-37 0.6102954565288456 2312 38-39 0.5232955719118451 2312 40-41 1.04676866255096 2312 42-43 0.859146402888264 2312 44-45 0.9197319094935743 2312 46-47 0.6664839314529942 2312 48-49 0.5243166540424653 2312 50-51 0.3860359456750011 2312 52-53 0.2080213636730086 2312 54-55 0.08466170867086831 2312 56-57 0.18970248941734624 2312 58-59 0.6773774973927793 2312 60-61 0.5235900457307316 2312 62-63 0.585855323019512 2312 64-65 0.19897177111100817 2312 66-67 0.22496691543065594 2312 68-69 0.02972798712394109 2312 70-71 0.6275723544843892 2312 72-73 0.8654338636226449 2312 74-75 0.9724207828011728 2312 76-77 1.0815193995328727 2312 78-79 0.9473264242774597 2312 80-81 1.0045676466797708 2312 82-83 1.0640276925757348 2312 84-85 1.2243931419495055 2312 86-87 1.2490843621008771 2312 88-89 0.9674776388982629 2312 90-91 1.1791606294434445 2312 92-93 1.1644261900241286 2312 94-95 1.804714515236217 2312 96-97 2.8097826391821705 2312 98-99 2.796636773913537 2312 100-101 2.442349393551279 2313 1 -0.06580479167200792 2313 2 -0.330969442808847 2313 3 -0.6333799626750611 2313 4 -0.01590639253574011 2313 5 -0.24413528289149156 2313 6 0.36269693891996013 2313 7 0.23756590059893057 2313 8 0.3977223115938102 2313 9 0.5805606204370406 2313 10-11 -0.6854504349904857 2313 12-13 0.1314304441184433 2313 14-15 0.21180319931279 2313 16-17 -0.4750991661161308 2313 18-19 0.027541553177414357 2313 20-21 -1.9605120481941114 2313 22-23 -0.29290380749001343 2313 24-25 0.4259302004882528 2313 26-27 0.17852734783512858 2313 28-29 0.08679856438491385 2313 30-31 0.548190220808074 2313 32-33 0.6359581364590667 2313 34-35 0.3284985683060597 2313 36-37 -0.6690984314648247 2313 38-39 0.16595734784264238 2313 40-41 0.47190930654496555 2313 42-43 0.6328397330176472 2313 44-45 0.07332539887813638 2313 46-47 -0.12480049923330938 2313 48-49 0.12711810807049062 2313 50-51 0.21873320064347723 2313 52-53 0.2570706621021266 2313 54-55 0.1172960625566013 2313 56-57 0.15988867191058276 2313 58-59 0.4391520146799728 2313 60-61 0.3431458038890298 2313 62-63 0.41762555266736 2313 64-65 0.3341861295199635 2313 66-67 0.6666614988700026 2313 68-69 0.4893358330327793 2313 70-71 0.6136581402531931 2313 72-73 0.7083725692267322 2313 74-75 0.8324069775448066 2313 76-77 0.7641963942275076 2313 78-79 0.41495710304375066 2313 80-81 0.24774276140945872 2313 82-83 0.30222961683058003 2313 84-85 0.20665008933875484 2313 86-87 0.05077161885980175 2313 88-89 0.5058408207978289 2313 90-91 0.6630978838465218 2313 92-93 0.634638875782569 2313 94-95 1.247668298253398 2313 96-97 2.46999546629136 2313 98-99 2.670998339702887 2313 100-101 2.2842108886652532 2314 1 -0.34467541985291916 2314 2 -0.17156344150311753 2314 3 -1.195494090162832 2314 4 -0.21943306440448396 2314 5 -0.6089267569350127 2314 6 -0.6408355041287166 2314 7 -0.48671958524424497 2314 8 -2.1109903666064014 2314 9 -2.0773502845681335 2314 10-11 -1.357563853104054 2314 12-13 -0.7734623141328427 2314 14-15 -0.6731278891918535 2314 16-17 -0.8782232981847713 2314 18-19 -2.2161080448238337 2314 20-21 -2.046537496692949 2314 22-23 -2.6947306264036186 2314 24-25 -2.1566253260778083 2314 26-27 -2.48862526019715 2314 28-29 -3.4003365136883303 2314 30-31 -1.9893687384077232 2314 32-33 -1.6194243905722985 2314 34-35 -1.9991733978267376 2314 36-37 -3.687763990385566 2314 38-39 -4.277235004382085 2314 40-41 -5.268711023157735 2314 42-43 -3.767125279204027 2314 44-45 -3.868920119139368 2314 46-47 -3.6196328885378684 2314 48-49 -1.8960730177785017 2314 50-51 -1.5294353514756835 2314 52-53 -2.828651198285815 2314 54-55 -2.234505447328349 2314 56-57 -1.9881180467047166 2314 58-59 -3.732536380078354 2314 60-61 -2.49991597538979 2314 62-63 -2.523425451337598 2314 64-65 -2.122629814326455 2314 66-67 -2.889077278289111 2314 68-69 -2.8113985496132834 2314 70-71 -2.1633795371354445 2314 72-73 -1.869313524273597 2314 74-75 -2.264158689465326 2314 76-77 -1.2574016454950403 2314 78-79 -0.8228131054040553 2314 80-81 -0.7277627879407405 2314 82-83 -0.7614739897961762 2314 84-85 -0.5755642162139587 2314 86-87 -0.33069315469953864 2314 88-89 -0.03253692457049695 2314 90-91 0.3479716212506183 2314 92-93 0.20401086319658823 2314 94-95 0.60187626993614 2314 96-97 1.8202345494432421 2314 98-99 1.6779165416436879 2314 100-101 1.3407173529879408 2315 1 -2.0089291072483384 2315 2 -1.773303835587548 2315 3 -1.3290763439461237 2315 4 -1.6007227215016044 2315 5 -1.7367761358838152 2315 6 -2.8712619105149173 2315 7 -1.9480715503183355 2315 8 -1.0329664687678743 2315 9 -0.6025187484699543 2315 10-11 -3.2433354306835085 2315 12-13 -1.6869761865096748 2315 14-15 -1.1794441329109588 2315 16-17 -1.7261367555029494 2315 18-19 -1.3074602926689352 2315 20-21 -0.9703534545674444 2315 22-23 -0.9367026960046232 2315 24-25 -3.1919876848306146 2315 26-27 -4.421745613685502 2315 28-29 -5.835517407220131 2315 30-31 -8.477307940182051 2315 32-33 -6.826982613737634 2315 34-35 -6.080238313444177 2315 36-37 -3.9557583693896916 2315 38-39 -6.627374361311638 2315 40-41 -4.157405592356103 2315 42-43 -3.962384807664929 2315 44-45 -3.2022346010721456 2315 46-47 -2.2369508915844207 2315 48-49 -2.8869376700500737 2315 50-51 -2.8346589926073094 2315 52-53 -1.9974796288206598 2315 54-55 -3.2686070162156717 2315 56-57 -2.8226994656988147 2315 58-59 -2.0096906955942444 2315 60-61 -2.3640973717622096 2315 62-63 -2.8547672981273493 2315 64-65 -1.6880230688855242 2315 66-67 -2.0636373542192707 2315 68-69 -1.50779285042508 2315 70-71 -1.863132051031183 2315 72-73 -1.1890611450014497 2315 74-75 -1.559248678265135 2315 76-77 -0.8687113793337566 2315 78-79 -0.9671504930179502 2315 80-81 -0.6208286280964366 2315 82-83 -0.633134064619842 2315 84-85 -0.7216689226300339 2315 86-87 -1.037608995900257 2315 88-89 -1.4153713719806227 2315 90-91 -1.4147582992415497 2315 92-93 -0.7788089538874523 2315 94-95 -0.3060153771312173 2315 96-97 0.92282694487022 2315 98-99 0.8613162851826779 2315 100-101 0.47797317100143744 2316 1 -3.849562150578624 2316 2 -2.380808712418631 2316 3 -2.559600614235805 2316 4 -3.2918438233213294 2316 5 -2.673093918830734 2316 6 -6.480207000963084 2316 7 -3.806938123473305 2316 8 -4.704282443627328 2316 9 -7.110468702610852 2316 10-11 -5.963256425394384 2316 12-13 -3.942740481974944 2316 14-15 -4.924501922890023 2316 16-17 -4.172103719430041 2316 18-19 -2.7797214780986295 2316 20-21 -3.0991011694326787 2316 22-23 -4.235206675673965 2316 24-25 -2.9025691882835005 2316 26-27 -4.121397885306536 2316 28-29 -3.540369337994779 2316 30-31 -3.6140018553733597 2316 32-33 -3.739944786545749 2316 34-35 -6.006701081231945 2316 36-37 -6.211640284385808 2316 38-39 -5.9604084321861635 2316 40-41 -4.850966048169326 2316 42-43 -4.3929529975879404 2316 44-45 -3.7345286699196087 2316 46-47 -4.091441142301711 2316 48-49 -4.894014236610843 2316 50-51 -5.727612706819379 2316 52-53 -3.8900279310327797 2316 54-55 -4.273867987677921 2316 56-57 -3.74584573809814 2316 58-59 -3.9750955382656095 2316 60-61 -3.944920132893209 2316 62-63 -3.132023434676114 2316 64-65 -3.5921844228615036 2316 66-67 -3.994861836098984 2316 68-69 -3.2290170445123216 2316 70-71 -2.9108411604135895 2316 72-73 -2.8866963667956007 2316 74-75 -4.112398316585075 2316 76-77 -3.8844348583192776 2316 78-79 -4.182799646470144 2316 80-81 -4.137359177267033 2316 82-83 -3.3478816768808066 2316 84-85 -3.444432806426942 2316 86-87 -3.1610131553773257 2316 88-89 -3.208348484971701 2316 90-91 -2.688530895642643 2316 92-93 -2.5215969371754685 2316 94-95 -2.217021186785278 2316 96-97 -0.741770952743229 2316 98-99 -0.15819854676528955 2316 100-101 -0.4223261331664254 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11738.0 3 2617.0 4 2323.0 5 2487.0 6 3039.0 7 3862.0 8 5059.0 9 6401.0 10 8008.0 11 10315.0 12 12735.0 13 15567.0 14 17891.0 15 20514.0 16 23094.0 17 23634.0 18 18596.0 19 16323.0 20 16640.0 21 17757.0 22 18931.0 23 21016.0 24 22422.0 25 24681.0 26 26943.0 27 29548.0 28 32880.0 29 35283.0 30 39109.0 31 43501.0 32 48522.0 33 54069.0 34 59934.0 35 62977.0 36 59417.0 37 44533.0 38 10228.0 39 65.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.882251708055826 21.33776194491771 14.84078223507208 17.939204111954382 2 15.447148875244363 23.784038657452022 40.4115843865011 20.35722808080252 3 18.51424768657958 32.59923469241405 30.713617770740463 18.172899850265907 4 11.14797329397967 19.538244737862833 39.188147080492165 30.12563488766534 5 10.831552784197445 41.583261996617146 32.48478978810127 15.100395431084143 6 24.39638167520222 40.24956021724637 19.176019819772268 16.178038287779145 7 24.24549488810522 38.761921920956986 21.38274200452376 15.60984118641403 8 26.126189191980632 32.52148865605527 20.76636178978414 20.585960362179964 9 29.6253067164299 16.815175950218404 21.284570872440394 32.2749464609113 10-11 23.403570401849525 29.311643829721064 26.26840732029354 21.01637844813587 12-13 27.152337261045073 28.68027164042932 26.94628301903032 17.221108079495277 14-15 24.23576188577379 28.343063034117545 28.702684249933302 18.718490830175362 16-17 18.543007600072983 32.1354105106611 29.00248898660721 20.319092902658706 18-19 17.915043823228807 29.21324779678739 35.408067055020496 17.463641324963316 20-21 20.26699670042777 27.771801401112718 34.787066227948316 17.17413567051119 22-23 19.699839527827137 28.26124525257861 34.134641607362774 17.90427361223148 24-25 18.48853040976071 29.13787568959092 35.10015613595945 17.273437764688925 26-27 18.68986063862288 28.783683210709714 35.36808906999768 17.158367080669727 28-29 17.845460476142346 28.560156386390155 36.10250337673316 17.491879760734335 30-31 17.809768117889533 30.043201890018086 35.18062616611509 16.9664038259773 32-33 17.146350385712918 30.1405082692967 34.75143296382459 17.961708381165792 34-35 17.85207634954252 29.399809740439803 34.842875112453505 17.90523879756417 36-37 18.325683280942528 29.168685377340076 34.3930894654301 18.112541876287285 38-39 18.192997755786642 29.330990180920686 34.723130439375524 17.752881623917148 40-41 17.39247992367293 29.471801292439565 35.34524279023202 17.790475993655484 42-43 17.160074123060813 29.9226507031128 34.29224876691191 18.625026406914476 44-45 17.36004905000069 29.98491299136114 33.71676609855009 18.938271860088086 46-47 17.678572659015167 29.826784693017412 32.61163159792642 19.883011050041 48-49 17.776136529402915 29.952240438037194 32.13346718568913 20.138155846870763 50-51 18.099450826861407 30.276780734539894 31.785298058967904 19.83847037963079 52-53 17.64209420799022 30.498238203605403 31.759733831719 20.099933756685378 54-55 17.42161980189374 29.97250502963781 31.830627305849085 20.775247862619363 56-57 18.037558313361924 30.478182489595053 30.650243878066135 20.83401531897689 58-59 17.85241127660955 29.302680293727988 31.267432705521443 21.57747572414102 60-61 18.264698874385303 29.48806205378033 30.893401044372933 21.35383802746144 62-63 18.999813419447435 28.889271618227486 31.172041621815573 20.93887334050951 64-65 18.59881459795353 28.7563037269844 31.52354300311419 21.121338671947875 66-67 18.20899000753109 29.078038610605976 31.09208822394886 21.620883157914072 68-69 18.21319275936122 29.21341606577723 31.072255412023296 21.501135762838253 70-71 18.072494576483493 29.005236082989704 30.94553176294856 21.976737577578245 72-73 18.133698880616727 28.47398174713837 30.497795919867503 22.894523452377396 74-75 17.75951874572473 28.860955352225037 30.64739474716518 22.732131154885053 76-77 17.92109898013228 28.30940008524748 31.19655517476307 22.57294575985717 78-79 17.80708299372712 27.786077667438068 31.34268090456735 23.06415843426746 80-81 17.6244880609789 28.735905521761357 30.67228435376494 22.967322063494798 82-83 18.070542445975395 28.0416734157933 30.15038777322475 23.737396365006553 84-85 17.77472374429355 27.458906945718287 30.128799682523454 24.63756962746471 86-87 18.20311243826806 27.347593889973588 30.388078557482483 24.061215114275868 88-89 18.761912254598222 26.48550373008977 30.493361870104096 24.259222145207907 90-91 18.263793375733993 26.747218353839745 30.70765078316799 24.281337487258266 92-93 18.674792900495753 26.809737237478693 30.40560719323314 24.109862668792413 94-95 18.411793490996477 26.48188267619936 30.410787228274604 24.69553660452956 96-97 18.80148498566707 25.86256050376729 29.571202556431018 25.764751954134617 98-99 18.905268481640487 25.658258475720846 29.517118111231806 25.91935493140686 100-101 19.471952885699192 25.423375172221142 30.12009065571349 24.98458128636618 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4278.0 1 2885.5 2 1676.5 3 1868.0 4 1585.0 5 1582.0 6 2583.5 7 4856.0 8 7101.0 9 7439.5 10 5929.5 11 4411.0 12 4016.0 13 4004.0 14 3892.0 15 3576.5 16 3416.5 17 3333.5 18 3253.0 19 3380.0 20 3585.5 21 3790.0 22 4141.5 23 4481.0 24 4857.0 25 5600.0 26 6736.5 27 8082.5 28 9114.5 29 10385.5 30 12449.0 31 14719.0 32 17774.0 33 21668.5 34 25257.5 35 28349.0 36 31793.5 37 34574.0 38 34731.0 39 33511.0 40 31889.0 41 30485.0 42 29164.5 43 28915.0 44 29392.0 45 28691.5 46 27623.5 47 28420.0 48 33584.0 49 32517.0 50 24804.0 51 19469.5 52 15628.5 53 13411.0 54 12076.5 55 11260.5 56 10847.5 57 10583.0 58 10113.0 59 9322.0 60 8771.5 61 8147.0 62 6755.0 63 4936.0 64 3436.0 65 2697.5 66 2285.0 67 1821.0 68 1397.5 69 1076.0 70 817.0 71 690.5 72 635.0 73 601.0 74 506.5 75 406.5 76 300.5 77 194.5 78 140.0 79 101.5 80 75.0 81 50.5 82 26.0 83 16.0 84 17.5 85 12.5 86 5.5 87 2.0 88 2.0 89 2.0 90 2.5 91 2.0 92 1.0 93 0.5 94 1.0 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.12078028187413412 2 0.11493607468667602 3 0.12765581974173187 4 0.14266741075265366 5 0.13808371884092183 6 0.13819831113871514 7 0.14438629521955312 8 0.1454176258996928 9 0.15275153295846372 10-11 0.14902728328018158 12-13 0.14742299111107546 14-15 0.13596376133174587 16-17 0.13980260330782127 18-19 0.15750711331688552 20-21 0.18758759148762577 22-23 0.20529210149669003 24-25 0.222710130761271 26-27 0.22609060354617325 28-29 0.22861163409762575 30-31 0.22683545348182965 32-33 0.2290127071399023 34-35 0.1997916712026118 36-37 0.18804596067879895 38-39 0.20082300188275143 40-41 0.19142643346370117 42-43 0.191942098803771 44-45 0.19606742152432965 46-47 0.21692321972270953 48-49 0.23468502588067044 50-51 0.24849339776476265 52-53 0.273016149492528 54-55 0.2656249462848604 56-57 0.23371099134942747 58-59 0.22798137645976263 60-61 0.20643802447462295 62-63 0.1972706406511593 64-65 0.18867621831666206 66-67 0.1836914533626537 68-69 0.19005132589018162 70-71 0.19291613333501403 72-73 0.22929918788438552 74-75 0.2407011215148185 76-77 0.2580618546305029 78-79 0.24814962087138276 80-81 0.25141550135849167 82-83 0.24935283999821234 84-85 0.23416936054060064 86-87 0.2244863113770671 88-89 0.22568953050389673 90-91 0.2287835225443157 92-93 0.2364039103475699 94-95 0.24190434064164812 96-97 8.556778764672112 98-99 0.30641980429927385 100-101 0.31793633022750006 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 872659.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.08314592469587 #Duplication Level Percentage of deduplicated Percentage of total 1 84.09039103679049 36.22878587902782 2 6.929071775605419 5.970524208621996 3 2.585822500691856 3.3421610439800764 4 1.395783596128939 2.4053899340527938 5 0.8960633815624185 1.930261471281506 6 0.6060228343708775 1.5665622124138987 7 0.4564494062881553 1.376569346484737 8 0.3609395121034936 1.2440327735954677 9 0.26855224928272775 1.0413068169827753 >10 1.9900101720802459 16.976784594574536 >50 0.2642945233930335 7.8292627062492794 >100 0.14724582840961187 11.254915312073535 >500 0.004542974742205497 1.3453259689566532 >1k 0.00374127331711041 3.0309842925761963 >5k 5.344676167300585E-4 1.6802470852334956 >10k+ 5.344676167300585E-4 2.7768863538952093 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14064 1.6116260761649168 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10055 1.1522255543115927 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7988 0.9153632747728494 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6606 0.7569967192225141 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3918 0.4489726227541342 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2970 0.3403391244460895 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2482 0.28441808312296096 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2328 0.26677086926279336 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1890 0.21657944282932967 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1776 0.20351592088089393 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1623 0.1859832993185196 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1545 0.1770451000906425 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1521 0.1742948849436034 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1370 0.1569914479768157 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1369 0.15687685567902238 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1353 0.15504337891432965 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1136 0.1301768502931844 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1045 0.11974895119399444 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 910 0.10427899099189947 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.1459229779329612E-4 0.0 10-11 0.0 0.0 0.0 2.864807444832403E-4 0.0 12-13 0.0 0.0 0.0 4.5836919117318446E-4 0.0 14-15 0.0 0.0 0.0 6.875537867597767E-4 0.0 16-17 0.0 0.0 0.0 0.0012032191268296093 0.0 18-19 0.0 0.0 0.0 0.002005365211382682 0.0 20-21 0.0 0.0 0.0 0.0036669535293854757 0.0 22-23 0.0 0.0 0.0 0.01300622579953911 0.0 24-25 0.0 0.0 0.0 0.033575543253435765 0.0 26-27 0.0 0.0 0.0 0.0619944331061732 0.0 28-29 0.0 0.0 0.0 0.12593693527483243 0.0 30-31 0.0 0.0 0.0 0.28413160237847773 0.0 32-33 0.0 0.0 0.0 0.4808292815406705 0.0 34-35 0.0 0.0 0.0 0.7761909291028912 0.0 36-37 0.0 0.0 0.0 1.1825352170779193 0.0 38-39 0.0 0.0 0.0 1.7249005625335898 0.0 40-41 0.0 0.0 0.0 2.3385423172166906 0.0 42-43 0.0 0.0 0.0 2.876381266909526 0.0 44-45 0.0 0.0 0.0 3.4293463999110765 0.0 46-47 0.0 0.0 0.0 4.093007692580951 0.0 48-49 0.0 0.0 0.0 4.799411912327725 0.0 50-51 0.0 0.0 0.0 5.530625364546747 0.0 52-53 0.0 0.0 0.0 6.173087082124862 0.0 54-55 0.0 0.0 0.0 6.797156735907153 0.0 56-57 0.0 0.0 0.0 7.476517173374709 0.0 58-59 0.0 0.0 0.0 8.228872904536594 0.0 60-61 0.0 0.0 0.0 9.09318531064253 0.0 62-63 0.0 0.0 0.0 9.852301987374222 0.0 64-65 0.0 0.0 0.0 10.549137750255255 0.0 66-67 0.0 0.0 0.0 11.30917116536929 0.0 68-69 0.0 0.0 0.0 12.075277972266372 0.0 70-71 0.0 0.0 0.0 12.951679865789501 0.0 72-73 0.0 0.0 0.0 13.82091974070055 0.0 74-75 0.0 0.0 0.0 14.650396088277322 5.729614889664806E-5 76-77 0.0 0.0 0.0 15.450651399916806 1.1459229779329612E-4 78-79 0.0 0.0 0.0 16.289123242870353 1.1459229779329612E-4 80-81 0.0 0.0 0.0 17.176067627790466 1.1459229779329612E-4 82-83 0.0 0.0 0.0 18.02691543890569 1.1459229779329612E-4 84-85 0.0 0.0 0.0 18.902572482493163 1.1459229779329612E-4 86-87 0.0 0.0 0.0 19.661631863075954 1.1459229779329612E-4 88-89 0.0 0.0 0.0 20.33623672018509 1.1459229779329612E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 220 0.0 40.64409 9 GTACAGG 240 0.0 35.292126 1 CGTCACT 195 0.0 33.783916 1 CTTTAGG 300 0.0 29.802242 2 TAGGCAT 305 0.0 29.317047 5 CCTTTAG 305 0.0 29.31368 1 GTACATG 10670 0.0 27.386957 1 TACATGG 10725 0.0 26.895508 2 GTATCAA 17200 0.0 26.811071 1 CATGGGG 2530 0.0 26.410973 4 GGCATTC 345 0.0 25.917967 7 GTCGGGA 660 0.0 25.667002 2 ACATGGG 11030 0.0 25.511866 3 CCCTATA 130 1.07740725E-5 25.337938 2 TTAGGCA 410 0.0 25.249653 4 CATGGGA 7705 0.0 24.551044 4 GTAATAC 135 1.443498E-5 24.399496 3 TCGGGAG 715 0.0 24.350746 3 GAGTACT 9380 0.0 24.28128 12-13 CATGGAT 215 1.8517312E-9 24.07525 4 >>END_MODULE