FastQCFastQC Report
Fri 27 May 2016
SRR939807_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939807_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2189784
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22800.10411985839699257No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31100.037.2127151
GTATCAA48900.036.6644251
CAACGCA62600.029.6287985
TCAACGC62200.029.5905444
ATCAACG63050.029.3400823
TATCAAC62900.029.3326282
AACGCAG64850.028.381376
TACATGG76050.025.320882
GTACATG77400.024.9410881
ACGCAGA75450.023.891097
CGCAGAG76600.023.406958
ACATGGG78600.022.9923953
GCAGAGT84200.020.3369989
AGAGTAC67950.020.207210-11
CGTACGC1101.2655837E-619.55792490-91
GAGTACA55350.018.4239521
CATGGGA34250.018.2820534
TAAGGTG10400.018.2447645
GTACTAG5000.018.0237661
GTATAGG6600.017.9662741
Produced by FastQC (version 0.11.5)