FastQCFastQC Report
Fri 27 May 2016
SRR939807_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939807_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2189784
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25010.11421217800477125No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29400.040.105721
GTATCAA47950.035.8035771
ATCAACG57800.029.0426713
TCAACGC57900.028.9925124
CAACGCA59300.028.3080355
TATCAAC60800.027.6096442
AACGCAG63400.026.4586476
TACATGG71200.023.245642
GTACATG73050.023.2431981
ACGCAGA73850.022.7146667
CGCAGAG74800.022.426188
ACATGGG71850.021.98533
GCAGAGT86450.018.9679819
GAGTACA56650.018.7325231
AGAGTAC70500.018.64755810-11
CATGGGG44550.018.5227184
TAAGGTG11150.017.7618755
GAGTACT39550.017.69240412-13
AGTACAT57800.016.724012
CAGAGTA80950.016.56045710-11
Produced by FastQC (version 0.11.5)