##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939807_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2189784 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.411041910983002 31.0 31.0 34.0 30.0 34.0 2 31.623909481483103 31.0 31.0 34.0 30.0 34.0 3 31.695676833879507 31.0 31.0 34.0 30.0 34.0 4 35.50124441497426 37.0 35.0 37.0 33.0 37.0 5 35.2254386734034 37.0 35.0 37.0 32.0 37.0 6 35.24432090105691 37.0 35.0 37.0 32.0 37.0 7 35.159853665932346 37.0 35.0 37.0 32.0 37.0 8 35.094258611808286 37.0 35.0 37.0 32.0 37.0 9 36.70795932384198 39.0 35.0 39.0 32.0 39.0 10-11 36.557797481395426 38.5 35.0 39.0 32.0 39.0 12-13 36.500210294713995 38.0 35.0 39.0 32.0 39.0 14-15 37.5752402976732 39.0 36.0 41.0 32.0 41.0 16-17 37.49203779916193 39.0 36.0 41.0 32.0 41.0 18-19 37.43254357507407 39.0 36.0 41.0 32.0 41.0 20-21 37.33896151401234 39.0 36.0 40.5 31.5 41.0 22-23 37.19521628617252 39.0 36.0 40.0 31.0 41.0 24-25 37.038550605904504 39.0 36.0 40.0 31.0 41.0 26-27 36.75158120618289 39.0 35.5 40.0 30.0 41.0 28-29 36.526948320016956 38.0 35.0 40.0 30.0 41.0 30-31 36.2960702973444 38.0 35.0 40.0 30.0 41.0 32-33 36.29836983921702 38.0 35.0 40.0 30.0 41.0 34-35 36.41760717038758 38.0 35.0 40.0 30.0 41.0 36-37 36.4086624525524 38.0 35.0 40.0 30.0 41.0 38-39 36.28507674729562 38.0 35.0 40.0 30.0 41.0 40-41 36.131759799140006 38.0 35.0 40.0 29.5 41.0 42-43 35.95450761353631 38.0 35.0 40.0 29.0 41.0 44-45 35.754374175717786 38.0 34.0 40.0 28.5 41.0 46-47 35.52153363071426 38.0 34.0 40.0 27.5 41.0 48-49 35.253114462431 38.0 34.0 40.0 27.0 41.0 50-51 34.99972006371405 38.0 33.5 40.0 26.5 41.0 52-53 34.70841987154897 38.0 33.0 40.0 26.0 41.0 54-55 34.39035539578333 37.5 33.0 40.0 25.5 41.0 56-57 32.97696325299664 36.5 31.5 39.5 18.0 41.0 58-59 29.34207118144986 34.0 23.5 38.5 8.0 40.0 60-61 29.367665486641606 34.0 24.0 38.0 9.5 40.0 62-63 31.38323505880032 35.0 29.5 38.0 14.5 40.0 64-65 30.909967604110726 35.0 29.5 38.0 7.5 40.0 66-67 25.246737577770226 28.5 18.0 36.0 2.0 39.0 68-69 17.40614393931091 13.0 7.0 29.0 2.0 35.5 70-71 16.27797170862514 13.0 7.5 25.5 2.0 33.0 72-73 18.337056074937074 17.5 7.0 30.0 2.0 34.0 74-75 19.073776682997043 17.5 7.5 30.5 2.0 35.0 76-77 20.112197139078557 23.0 8.5 30.5 2.0 34.5 78-79 23.251102848500125 27.5 12.0 33.5 2.0 35.5 80-81 26.01833080340344 31.5 22.0 34.5 2.0 36.0 82-83 27.122506375057995 33.0 26.0 35.0 2.0 36.0 84-85 27.15711686632106 33.0 26.0 35.0 2.0 36.0 86-87 26.878803571493812 33.0 26.0 35.0 2.0 35.5 88-89 26.435456876111985 33.0 25.0 35.0 2.0 35.0 90-91 24.70487271803977 31.5 12.5 34.5 2.0 35.0 92-93 22.445780268738837 30.5 2.0 34.0 2.0 35.0 94-95 17.860745854385637 20.0 2.0 33.0 2.0 35.0 96-97 16.47675113161846 13.5 2.0 32.5 2.0 34.5 98-99 15.338031285277452 2.0 2.0 31.5 2.0 34.0 100-101 14.726392648772665 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4742456934796273 1101 2 0.3419946264030429 1101 3 0.471208733953393 1101 4 0.33617285046200607 1101 5 0.4185662997625812 1101 6 0.4020499494958827 1101 7 0.4762039322967766 1101 8 0.5563296210734734 1101 9 0.49814289108868337 1101 10-11 0.48575616316884407 1101 12-13 0.5607948093107495 1101 14-15 0.6709639694140392 1101 16-17 0.5892477890611048 1101 18-19 0.670712073124669 1101 20-21 0.5603487102068883 1101 22-23 0.6205789257902055 1101 24-25 0.5418470302091407 1101 26-27 0.6625277634438973 1101 28-29 0.7882251924324564 1101 30-31 0.8367254623054095 1101 32-33 0.7559861890357595 1101 34-35 0.8036147595320102 1101 36-37 0.819548130726254 1101 38-39 0.6933711623938663 1101 40-41 0.7659405329427287 1101 42-43 0.7308843378175212 1101 44-45 0.558609262500525 1101 46-47 0.5104844258286079 1101 48-49 0.5059271041757256 1101 50-51 0.43125675761516646 1101 52-53 0.7367717227810644 1101 54-55 0.5315004646629262 1101 56-57 1.2018979182773961 1101 58-59 -8.196226524148926 1101 60-61 -9.551125864990116 1101 62-63 -2.7494786015906207 1101 64-65 -0.26525400016154066 1101 66-67 -1.6678465918407035 1101 68-69 -4.1051860375774325 1101 70-71 -4.646570790610555 1101 72-73 -7.223262985101371 1101 74-75 -8.247836284131004 1101 76-77 -9.124083813192296 1101 78-79 -12.098503021744818 1101 80-81 -8.273616717746162 1101 82-83 -4.597082896743867 1101 84-85 -3.911967402714673 1101 86-87 -3.6157469819060992 1101 88-89 -3.6278802942803416 1101 90-91 -9.00831934808259 1101 92-93 -14.896953529085712 1101 94-95 -12.86759442066873 1101 96-97 -12.618718924608508 1101 98-99 -12.24290837380058 1101 100-101 -12.041842147279487 1102 1 0.500979701725484 1102 2 0.5257116663839589 1102 3 0.47201400323569587 1102 4 0.41246544321305834 1102 5 0.547359399778216 1102 6 0.5040709169966391 1102 7 0.49323785569495726 1102 8 0.6076071865084813 1102 9 0.6625110173359303 1102 10-11 0.6264383164783922 1102 12-13 0.7830989045773649 1102 14-15 0.7961020110381156 1102 16-17 0.8581973510767966 1102 18-19 0.7925743928339415 1102 20-21 0.8850249398522294 1102 22-23 0.8823285077999614 1102 24-25 0.7173393945534627 1102 26-27 0.8890602637478295 1102 28-29 0.6917413514038842 1102 30-31 0.7112082960060775 1102 32-33 0.6807460379111561 1102 34-35 0.8270965558289518 1102 36-37 0.8352508817311417 1102 38-39 0.7599604932748178 1102 40-41 0.6982331019720149 1102 42-43 0.6454419205016464 1102 44-45 0.5703979218860269 1102 46-47 0.5723546076093626 1102 48-49 0.769986805086603 1102 50-51 0.59173303139994 1102 52-53 0.5185003279707914 1102 54-55 0.6240416208703436 1102 56-57 1.0916857397761746 1102 58-59 -8.311355886147755 1102 60-61 -9.550271669562573 1102 62-63 -2.493294103850328 1102 64-65 0.3695036417422841 1102 66-67 -1.0461122002341412 1102 68-69 -3.4884141549883196 1102 70-71 -4.195316057376656 1102 72-73 -6.421787914772684 1102 74-75 -7.462243360673099 1102 76-77 -8.790378062231033 1102 78-79 -11.821290448022014 1102 80-81 -7.5820030826437055 1102 82-83 -3.2668210380840854 1102 84-85 -2.2341488225556425 1102 86-87 -2.0585146174516424 1102 88-89 -2.267307523734644 1102 90-91 -8.134344202564371 1102 92-93 -14.415111906618488 1102 94-95 -12.50684860830198 1102 96-97 -12.381234380822574 1102 98-99 -11.897121253788345 1102 100-101 -11.592674717774438 1103 1 0.3456897021552656 1103 2 0.3799441328377675 1103 3 0.3115925199337859 1103 4 0.2457847589082931 1103 5 0.31004606485050346 1103 6 0.3596200817887478 1103 7 0.4470004193886652 1103 8 0.4122215324951881 1103 9 0.4566719753280495 1103 10-11 0.5197304345284266 1103 12-13 0.5016232236029055 1103 14-15 0.6278283541146266 1103 16-17 0.5751801069142886 1103 18-19 0.6641355389023218 1103 20-21 0.5504097980475464 1103 22-23 0.575640099742337 1103 24-25 0.5729499422057884 1103 26-27 0.56063646249752 1103 28-29 0.558887455566385 1103 30-31 0.6188841558442277 1103 32-33 0.7072362893464614 1103 34-35 0.7917522637871954 1103 36-37 0.38902267855860373 1103 38-39 0.36379674724645383 1103 40-41 0.51668851179668 1103 42-43 0.6043012151519136 1103 44-45 0.6003674607701655 1103 46-47 0.48568139206668803 1103 48-49 0.5412687156913591 1103 50-51 0.7067462096933994 1103 52-53 0.6597563755191729 1103 54-55 0.4264666448425629 1103 56-57 1.042112962426927 1103 58-59 -6.237774390581613 1103 60-61 -8.430943389296452 1103 62-63 -2.0772446759447583 1103 64-65 0.23575038932465375 1103 66-67 -1.4317126449051507 1103 68-69 -4.081675591890132 1103 70-71 -4.86886864027899 1103 72-73 -7.607321773949092 1103 74-75 -8.292460528502888 1103 76-77 -8.935361190684077 1103 78-79 -12.130614720408918 1103 80-81 -8.297395025058567 1103 82-83 -4.282144132989391 1103 84-85 -3.3226186948813528 1103 86-87 -3.05750246858063 1103 88-89 -3.2620982061249215 1103 90-91 -8.675314948336307 1103 92-93 -14.509567173319304 1103 94-95 -12.53803099847447 1103 96-97 -12.527670339786333 1103 98-99 -12.129086054368079 1103 100-101 -11.787886607912073 1104 1 0.220447648963777 1104 2 0.27177783206394324 1104 3 0.20514246600130548 1104 4 0.20934023290331538 1104 5 0.0632897036066069 1104 6 0.1800477636894513 1104 7 0.14322177421922788 1104 8 0.12709429892834123 1104 9 0.27113189368294144 1104 10-11 0.2911766123080284 1104 12-13 0.26261265137638645 1104 14-15 0.22671169885659026 1104 16-17 0.31565416362585097 1104 18-19 0.27921591976453186 1104 20-21 0.3260065266127441 1104 22-23 0.3582150330500937 1104 24-25 0.35790565535622676 1104 26-27 0.29372071760102614 1104 28-29 0.26653698809069937 1104 30-31 0.32530671785181653 1104 32-33 0.2739886640874616 1104 34-35 0.27653864614357104 1104 36-37 0.17554927195633496 1104 38-39 0.2574234169855387 1104 40-41 0.2313832410802874 1104 42-43 0.18956432694934477 1104 44-45 0.14083689494568574 1104 46-47 0.16759973409855178 1104 48-49 0.06683829120179041 1104 50-51 0.09993990768226269 1104 52-53 0.11380471786210222 1104 54-55 0.1725411820198275 1104 56-57 0.6564803257931828 1104 58-59 -6.877253072783475 1104 60-61 -8.61321372180798 1104 62-63 -2.347746101197739 1104 64-65 -0.11771886742194582 1104 66-67 -1.4056471894471976 1104 68-69 -4.023661113620658 1104 70-71 -4.873495694592224 1104 72-73 -7.932962581697296 1104 74-75 -8.602935681763418 1104 76-77 -8.99867003674094 1104 78-79 -12.066538376967003 1104 80-81 -8.40384725123543 1104 82-83 -4.596575464608215 1104 84-85 -3.597198031067798 1104 86-87 -3.3053338301242903 1104 88-89 -3.5231994610391872 1104 90-91 -9.449735189344214 1104 92-93 -15.457049602903394 1104 94-95 -13.000126029126577 1104 96-97 -12.622250902856406 1104 98-99 -11.828198318911994 1104 100-101 -11.455816738801094 1105 1 0.08453734391852663 1105 2 0.11980327854960038 1105 3 0.0694902133266524 1105 4 0.07067695483900849 1105 5 0.10410211569475791 1105 6 0.14531068229195654 1105 7 0.13286717864927056 1105 8 0.18783108906232826 1105 9 0.15601460683810586 1105 10-11 0.19485132779330883 1105 12-13 0.20335899637447596 1105 14-15 0.1818963670293101 1105 16-17 0.12323154381709145 1105 18-19 0.20644000086366532 1105 20-21 0.2648953563017358 1105 22-23 0.19859054986523006 1105 24-25 0.16706198572009612 1105 26-27 0.19734774756565088 1105 28-29 0.07483391348461765 1105 30-31 0.07057084413230541 1105 32-33 0.1145222446395806 1105 34-35 0.04167218336136358 1105 36-37 0.12245939229789826 1105 38-39 0.24650973754590666 1105 40-41 0.13488994724513503 1105 42-43 0.06453742059411383 1105 44-45 0.07925304302121106 1105 46-47 0.027250768439238016 1105 48-49 0.04537181492199949 1105 50-51 0.03140879931802942 1105 52-53 0.007468493205578852 1105 54-55 -0.11007020851481286 1105 56-57 0.5581647701902739 1105 58-59 -6.4048483372649585 1105 60-61 -8.904916796414067 1105 62-63 -2.937621408445228 1105 64-65 -0.49022048357926096 1105 66-67 -1.806672650140829 1105 68-69 -4.2319015623085505 1105 70-71 -4.8690016228703605 1105 72-73 -6.373040352607887 1105 74-75 -6.962394464424003 1105 76-77 -8.50981717099211 1105 78-79 -12.061842995980117 1105 80-81 -8.491069307697927 1105 82-83 -4.5384374909498035 1105 84-85 -3.555603558680996 1105 86-87 -3.2904548820828587 1105 88-89 -3.4519317188287104 1105 90-91 -8.596047597880366 1105 92-93 -11.765235794881573 1105 94-95 -11.929000544279384 1105 96-97 -12.30615788323519 1105 98-99 -11.645846223945881 1105 100-101 -11.377003850745739 1106 1 0.11103938174423433 1106 2 0.12630943782056292 1106 3 0.04630193196932453 1106 4 0.052292828509081346 1106 5 0.00936230100714397 1106 6 -0.03982921787748239 1106 7 0.058572594533629285 1106 8 0.17273839470565377 1106 9 0.06429107949155366 1106 10-11 0.0705937603618807 1106 12-13 0.0698646213840135 1106 14-15 0.07604448719233403 1106 16-17 0.007556790722190954 1106 18-19 0.15612306935597786 1106 20-21 0.13130996606193435 1106 22-23 0.051683160266534856 1106 24-25 0.21585608136814471 1106 26-27 0.2308927819873503 1106 28-29 0.11181758798203134 1106 30-31 0.12531394071150714 1106 32-33 0.26167806579939423 1106 34-35 0.2770762567230918 1106 36-37 0.19566274745962886 1106 38-39 0.3057339518000006 1106 40-41 0.31565787171583537 1106 42-43 0.15969424540215726 1106 44-45 0.1369152104434761 1106 46-47 0.13086437913056415 1106 48-49 0.050735417981037756 1106 50-51 0.09782092857876279 1106 52-53 -0.05691058053776743 1106 54-55 -0.1852844855064717 1106 56-57 0.6834856132547955 1106 58-59 -6.958876859192571 1106 60-61 -8.85159846056207 1106 62-63 -2.6221699003015573 1106 64-65 -0.23492700233183328 1106 66-67 -1.749783349107414 1106 68-69 -4.137276423018966 1106 70-71 -4.884380051683841 1106 72-73 -6.0773702552196625 1106 74-75 -6.614927817601332 1106 76-77 -8.67670641478323 1106 78-79 -12.035672963466492 1106 80-81 -8.20145916588968 1106 82-83 -4.214083184039513 1106 84-85 -3.1529382620132616 1106 86-87 -2.956805952895394 1106 88-89 -3.1826889580709725 1106 90-91 -8.577468179920023 1106 92-93 -11.850390673451582 1106 94-95 -11.841701083120189 1106 96-97 -12.25250696964816 1106 98-99 -11.90753185689302 1106 100-101 -11.617177065930974 1107 1 0.14127916531552032 1107 2 0.10718170472113187 1107 3 0.0939486571816488 1107 4 -0.0019247250902552082 1107 5 0.05414632435964961 1107 6 0.050527304749955704 1107 7 0.1581876914697844 1107 8 0.14009678313836105 1107 9 0.030992502505661434 1107 10-11 0.2788588432663417 1107 12-13 0.2103439654284074 1107 14-15 0.2531054740142764 1107 16-17 0.3198772113679311 1107 18-19 0.20881621660195293 1107 20-21 0.27058722190739104 1107 22-23 0.3256462527829953 1107 24-25 0.2548603942081513 1107 26-27 0.3577754663985431 1107 28-29 0.47079987281264835 1107 30-31 0.40171584855317377 1107 32-33 0.26534010494020066 1107 34-35 0.18794207726056555 1107 36-37 0.1327379068867529 1107 38-39 0.3429619921878171 1107 40-41 0.14354379339898315 1107 42-43 0.18561942766749695 1107 44-45 0.25974044355172055 1107 46-47 0.22217229805904282 1107 48-49 0.322007535523241 1107 50-51 0.4005290468865539 1107 52-53 0.4019976709244091 1107 54-55 0.3836411089033902 1107 56-57 1.0488975288109614 1107 58-59 -6.906583527362216 1107 60-61 -9.009304506405226 1107 62-63 -2.4706287028749365 1107 64-65 -0.1757587240743952 1107 66-67 -1.6703994405917584 1107 68-69 -4.224176098117569 1107 70-71 -4.707711835071951 1107 72-73 -6.264049415384191 1107 74-75 -6.797595613849291 1107 76-77 -9.156109169223763 1107 78-79 -12.628222306324943 1107 80-81 -8.285366389926043 1107 82-83 -4.370643503328228 1107 84-85 -3.401420313764998 1107 86-87 -3.1349066946381186 1107 88-89 -3.2276943182982087 1107 90-91 -8.702562849869754 1107 92-93 -12.169706396119414 1107 94-95 -12.087201251618023 1107 96-97 -12.65277592037047 1107 98-99 -11.911573456483458 1107 100-101 -11.3694773255468 1108 1 -0.014917636520397792 1108 2 0.01469740150077925 1108 3 -0.04943658171108822 1108 4 -0.05661929001620791 1108 5 -0.18763423881883767 1108 6 0.023896758296551468 1108 7 -0.0279046839432624 1108 8 0.017451393313407948 1108 9 -0.06884468430923363 1108 10-11 0.039553294081358104 1108 12-13 -0.14599381562105407 1108 14-15 -0.023373670347623943 1108 16-17 -0.08888568810890973 1108 18-19 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1.7816662359021471 2312 86-87 1.5467787360680987 2312 88-89 1.7138682245946129 2312 90-91 3.3243311575778343 2312 92-93 5.37510709241479 2312 94-95 9.283397363834293 2312 96-97 9.882230479377782 2312 98-99 10.473583330839201 2312 100-101 9.551245862744171 2313 1 -0.36153165135088017 2313 2 -0.2924062659447628 2313 3 -0.2499539418606389 2313 4 -0.3632617110739389 2313 5 -0.44005009637867687 2313 6 -0.33580264370251456 2313 7 -0.3975056903519487 2313 8 -0.4352978716945728 2313 9 -0.34108158190962 2313 10-11 -0.49042990454134383 2313 12-13 -0.3943872771669277 2313 14-15 -0.6095026541244835 2313 16-17 -0.5164353435114606 2313 18-19 -0.4731087331817392 2313 20-21 -0.5799117752920893 2313 22-23 -0.5540737564630547 2313 24-25 -0.5635604295606242 2313 26-27 -0.6115706496852482 2313 28-29 -0.603661593938547 2313 30-31 -0.6033967557605848 2313 32-33 -0.46794108581770644 2313 34-35 -0.4667646007271813 2313 36-37 -0.6087409587776449 2313 38-39 -1.018125724058926 2313 40-41 -1.033349989085231 2313 42-43 -0.6382029397045912 2313 44-45 -0.7332300354352412 2313 46-47 -0.6778396409505518 2313 48-49 -0.8553712453856335 2313 50-51 -0.5554980169028525 2313 52-53 -0.6297496050360678 2313 54-55 -0.4702755728300758 2313 56-57 0.7547601928197736 2313 58-59 4.256030169771435 2313 60-61 3.8535160827028925 2313 62-63 1.1262317571850708 2313 64-65 1.048863633227743 2313 66-67 1.851872915299058 2313 68-69 0.31088911633831984 2313 70-71 2.7212119604803284 2313 72-73 8.110603952827834 2313 74-75 9.824606443017014 2313 76-77 8.785095022932499 2313 78-79 6.5110698896823145 2313 80-81 3.660262101662102 2313 82-83 2.1402845048253916 2313 84-85 1.6007405325391382 2313 86-87 1.5285307178695362 2313 88-89 1.5244112194950539 2313 90-91 2.9514526624510715 2313 92-93 5.099460100807338 2313 94-95 9.024208970462812 2313 96-97 9.65781893982869 2313 98-99 10.018354856057691 2313 100-101 9.099678262252983 2314 1 -0.49480705982341533 2314 2 -0.3988693320283403 2314 3 -0.4225653556842772 2314 4 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0.7461871713509609 2314 66-67 1.2675947047681966 2314 68-69 -0.5350338471318992 2314 70-71 2.261119891767404 2314 72-73 7.919030542914502 2314 74-75 9.613606489225596 2314 76-77 8.539891246857788 2314 78-79 6.21438766612518 2314 80-81 3.2451073142632616 2314 82-83 1.657492847217373 2314 84-85 1.1810152615182083 2314 86-87 1.2049713841518894 2314 88-89 1.3428099632275412 2314 90-91 2.659096244267918 2314 92-93 4.8057329541723455 2314 94-95 8.65323508285876 2314 96-97 9.15870769758283 2314 98-99 9.447648144980281 2314 100-101 8.769147113499969 2315 1 -0.6815248013573836 2315 2 -0.5085416603225958 2315 3 -0.5983761560474434 2315 4 -0.4026619030487808 2315 5 -0.7366237869141088 2315 6 -0.7768479479365027 2315 7 -0.751423015465285 2315 8 -0.847980940953093 2315 9 -1.0073357373535572 2315 10-11 -0.8458970307383495 2315 12-13 -0.6624674610745416 2315 14-15 -0.8847017395190804 2315 16-17 -0.9403748575119835 2315 18-19 -0.8449174894782985 2315 20-21 -0.9440033496011893 2315 22-23 -1.0226070463574075 2315 24-25 -0.9189215439535232 2315 26-27 -1.049309598899427 2315 28-29 -0.9465216690975353 2315 30-31 -0.9273063756257258 2315 32-33 -1.0001965197679752 2315 34-35 -1.1451711299404863 2315 36-37 -1.0516495248894273 2315 38-39 -1.175038210817398 2315 40-41 -0.934382997541114 2315 42-43 -1.0545901568688834 2315 44-45 -0.97577281109875 2315 46-47 -0.8500740157454416 2315 48-49 -0.8526882318181137 2315 50-51 -0.9059932292665636 2315 52-53 -0.9218317166480006 2315 54-55 -0.9113291312664415 2315 56-57 0.5247246221799031 2315 58-59 3.970986903828191 2315 60-61 3.6522891701047477 2315 62-63 1.1069284596013809 2315 64-65 0.9851470582722222 2315 66-67 1.5948491857600295 2315 68-69 5.746315993851127 2315 70-71 4.598466799730236 2315 72-73 9.001028918945824 2315 74-75 10.049127808671813 2315 76-77 9.004377456279318 2315 78-79 6.210015559527747 2315 80-81 2.9596492818316484 2315 82-83 1.3835116831491767 2315 84-85 1.0268644267246394 2315 86-87 1.0549120437211812 2315 88-89 1.22305735129736 2315 90-91 2.391607109516972 2315 92-93 4.504272738369092 2315 94-95 8.358225811422432 2315 96-97 9.245303540271589 2315 98-99 9.607803757771421 2315 100-101 8.97092603113213 2316 1 -0.4794340206111727 2316 2 -0.533715245984645 2316 3 -0.7225247865921354 2316 4 -0.348860500291174 2316 5 -0.6026644962889378 2316 6 -0.5321447727407786 2316 7 -0.5215485391865826 2316 8 -0.7331174304367565 2316 9 -0.8493990075981941 2316 10-11 -0.6779545178707664 2316 12-13 -0.6005611231700883 2316 14-15 -0.7969537068739214 2316 16-17 -0.9227706773403526 2316 18-19 -0.815159435522709 2316 20-21 -0.8347978640923088 2316 22-23 -0.9508783214456997 2316 24-25 -2.384234163190854 2316 26-27 -1.6534177802333332 2316 28-29 -1.1680439675230687 2316 30-31 -0.7856723543344089 2316 32-33 -0.7643166625671967 2316 34-35 -1.0158228017767783 2316 36-37 -1.042570168993059 2316 38-39 -1.2017297820019053 2316 40-41 -1.0821599739788965 2316 42-43 -1.160515468940865 2316 44-45 -1.1063682110597028 2316 46-47 -0.975861592890702 2316 48-49 -1.2003219008673014 2316 50-51 -1.225574243545843 2316 52-53 -1.055318998470156 2316 54-55 -1.023833466428961 2316 56-57 0.25494135098842463 2316 58-59 3.672760406055332 2316 60-61 3.641795525121818 2316 62-63 0.9179585897680944 2316 64-65 0.6166107937283876 2316 66-67 5.955738625380146 2316 68-69 8.596217194492983 2316 70-71 5.3017878219620975 2316 72-73 9.368772530105257 2316 74-75 10.189352209930767 2316 76-77 8.712385774579381 2316 78-79 6.125598734167529 2316 80-81 3.124423569980049 2316 82-83 1.6490765181784646 2316 84-85 1.3142513351744753 2316 86-87 1.3284801805318551 2316 88-89 1.258995604250213 2316 90-91 2.531202491453204 2316 92-93 4.438938011703126 2316 94-95 8.55211393263195 2316 96-97 9.158552513568292 2316 98-99 9.497056832886514 2316 100-101 8.4051393834544 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 14.0 4 37.0 5 95.0 6 201.0 7 500.0 8 1118.0 9 2006.0 10 3181.0 11 4638.0 12 6650.0 13 9146.0 14 11959.0 15 16658.0 16 22427.0 17 29771.0 18 33601.0 19 37555.0 20 40395.0 21 40457.0 22 38779.0 23 37133.0 24 38512.0 25 41300.0 26 55344.0 27 80586.0 28 113847.0 29 149003.0 30 173588.0 31 183351.0 32 186875.0 33 191341.0 34 192728.0 35 182009.0 36 149480.0 37 87995.0 38 26420.0 39 1080.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.35022584710364 19.294363117562447 14.051302851625143 24.30410818370877 2 16.203399242988876 20.21264449705526 38.93145249682143 24.652503763134433 3 19.72956285615382 23.618335545189918 29.601811012762397 27.050290585893865 4 13.512519956306193 16.37545072938701 36.05734629534238 34.054683018964425 5 13.892886078644892 37.47378724450417 34.34219407103914 14.291132605811793 6 33.71154415229996 35.768961687545435 16.638079372212054 13.881414787942555 7 29.55250380859482 31.827248714941746 20.175231895017955 18.445015581445475 8 26.68732934906157 35.68276587067999 19.039061968410483 18.590842811847967 9 26.35611548901627 16.089669118049997 19.413147598119266 38.14106779481447 10-11 25.6046486776778 26.01656145080976 27.25035893951184 21.1284309320006 12-13 25.992243983881515 23.044213493202985 27.636812580601557 23.326729942313946 14-15 22.759528984539312 24.905955142972157 25.145910809741345 27.188605062747186 16-17 22.975713586362858 27.976343785505787 25.994139147970756 23.0538034801606 18-19 22.94758291287124 26.794865266798297 27.599544880279904 22.658006940050555 20-21 23.634751190071714 26.08147652919192 27.5636546800963 22.720117600640062 22-23 23.248160549168322 26.033458094496993 27.477413297384583 23.240968058950106 24-25 23.146575187324412 26.25923378744205 27.511777417316047 23.08241360791749 26-27 22.945260686124033 26.496281131566246 27.38452145170814 23.173936730601582 28-29 22.952208045693297 26.731097730976483 27.372866204311443 22.943828019018778 30-31 23.110352366422532 26.313407379446712 27.2861275088413 23.290112745289456 32-33 22.836722610808835 26.389030646320272 27.241584574843014 23.53266216802788 34-35 23.418894557959042 26.48950854147092 27.150654809950414 22.94094209061962 36-37 23.51791660395225 26.139108830273212 27.20047653057239 23.142498035202145 38-39 23.038682651607186 26.459932244206808 27.18778285348592 23.31360225070009 40-41 23.357124405556277 26.252731397660206 27.246438248315023 23.143705948468487 42-43 23.11925310524746 26.20259675476459 27.146493312143665 23.531656827844287 44-45 23.08126450793007 26.304705874022915 27.367834655677697 23.246194962369316 46-47 23.280000785724123 26.43918278295719 26.98270287728063 23.298113554038064 48-49 23.059567601273145 26.529401217641755 27.007481496116498 23.4035496849686 50-51 23.039304357994713 26.57066233260795 27.07886817481678 23.311165134580563 52-53 23.342789286034844 26.339445482713785 26.955494596723522 23.362270634527846 54-55 22.931556159274248 26.386576921873566 27.103236581272395 23.578630337579792 56-57 22.820981652441873 26.450338843058358 27.223631845119133 23.505047659380633 58-59 21.23314066397192 26.16927402617153 28.918993971902314 23.678591337954238 60-61 22.671496101944904 26.313704114257447 27.454766987000372 23.560032796797273 62-63 22.763264370419623 26.30693733057628 27.301582444962406 23.62821585404169 64-65 21.828291827153063 26.441199095208866 27.78752409010959 23.942984987528483 66-67 21.75526012693276 26.237001527890076 28.460166120120366 23.547572225056797 68-69 22.30753254189993 25.44758386733771 29.03332398507878 23.211559605683583 70-71 21.459389358164096 25.78433331434862 29.006677640455 23.749599687032287 72-73 21.375541919702123 26.009190939535326 28.915374210182716 23.699892930579836 74-75 21.91131312986055 26.235622032991362 28.12135629246474 23.73170854468335 76-77 20.550087235534097 26.443658036706346 28.71413714404993 24.29211758370963 78-79 21.64522702690184 26.67656958731741 27.419105192085397 24.25909819369535 80-81 22.39246517570951 26.718244169845452 26.534549833567677 24.354740820877357 82-83 22.678333594775182 26.684147878816113 26.41096968895727 24.226548837451443 84-85 22.640497875589556 26.69904885596022 26.18991188171984 24.47054138673038 86-87 22.593304669286464 26.6203444709063 26.282541038070818 24.503809821736418 88-89 22.607240078126463 26.60185963632013 26.140786552822814 24.650113732730595 90-91 22.076883644718652 26.73457338227621 26.44549307507117 24.743049897933965 92-93 21.888212339827902 26.642893413787267 26.611213533827705 24.857680712557126 94-95 20.292723074524492 26.645257458717918 28.036169733397298 25.02584973336029 96-97 20.17319533646508 26.392051387789966 28.256947276077536 25.177805999667413 98-99 20.51734557364949 26.500738109517 27.54168012178807 25.44023619504544 100-101 20.460248993173803 26.578721359388464 27.36843167131544 25.592597976122295 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 42.0 1 32.5 2 22.5 3 31.0 4 41.0 5 46.0 6 68.5 7 97.0 8 139.0 9 202.0 10 246.5 11 290.0 12 351.5 13 423.0 14 488.5 15 586.0 16 720.5 17 861.5 18 1024.5 19 1211.5 20 1459.0 21 1815.0 22 2302.5 23 3030.0 24 4013.0 25 5395.5 26 7396.0 27 9918.0 28 12655.5 29 15949.5 30 19967.5 31 24434.5 32 29461.5 33 34128.0 34 38411.0 35 42875.5 36 47668.0 37 52210.5 38 56391.5 39 60610.0 40 64999.5 41 69103.5 42 72571.0 43 75693.5 44 79095.0 45 83018.5 46 86118.0 47 89028.5 48 91013.5 49 91444.5 50 91258.5 51 90157.0 52 88770.0 53 86872.5 54 83534.5 55 77829.5 56 71078.5 57 63820.0 58 55571.0 59 46987.5 60 38397.5 61 30458.5 62 23338.0 63 17348.5 64 12634.0 65 9237.0 66 6747.5 67 4917.0 68 3511.0 69 2457.0 70 1804.5 71 1263.0 72 876.0 73 651.5 74 456.0 75 293.0 76 170.0 77 103.5 78 64.0 79 40.5 80 23.5 81 14.5 82 8.0 83 4.0 84 2.5 85 1.5 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021508970747799783 2 0.005479992547210136 3 0.003196662319205913 4 0.0 5 0.06580557717108172 6 0.0 7 0.0 8 3.1966623192059126E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.054997205203801E-4 16-17 0.0 18-19 0.0013928314390825763 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.283330228004223E-4 28-29 0.002443163343964519 30-31 0.009247487423417104 32-33 4.794993478808869E-4 34-35 0.0012101650208422383 36-37 0.028655794361452998 38-39 0.027331462829210555 40-41 0.030025792498255537 42-43 0.03045962524157634 44-45 0.031030457798577396 46-47 0.03290278858554086 48-49 0.03272012216730052 50-51 0.02794796199077169 52-53 0.02331280162792312 54-55 0.017832809080712983 56-57 0.14732046631083248 58-59 0.7555311391443175 60-61 0.30340892069720116 62-63 3.653328364806757E-4 64-65 0.14622446780139045 66-67 1.412216912718332 68-69 2.7062714861374455 70-71 2.17875370356163 72-73 1.8591331382455987 74-75 1.55716271559204 76-77 1.1134431523839794 78-79 0.423900256828984 80-81 2.283330228004223E-4 82-83 2.054997205203801E-4 84-85 0.0 86-87 0.0022376636234441387 88-89 4.566660456008446E-5 90-91 0.22486236085385589 92-93 0.17599909397456553 94-95 1.2902185786360665 96-97 1.2762674309429605 98-99 1.0085012951049053 100-101 0.40739177928051346 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2189784.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.35418411979658 #Duplication Level Percentage of deduplicated Percentage of total 1 74.2691135320608 40.368370713357095 2 14.130223569492092 15.360735471001249 3 4.904719017940972 7.997760016710945 4 2.265273034689298 4.925082704364499 5 1.2436190404570806 3.379794914994446 6 0.7354734970852874 2.3985637125482637 7 0.46244886624786163 1.759522157541922 8 0.32974769774884494 1.43385336612158 9 0.23955093433022048 1.1718536031588696 >10 1.288061438210067 12.713401378876762 >50 0.09148364445171561 3.411793044025243 >100 0.03699493497778193 3.426204493067711 >500 0.0021938615386473794 0.7787651950017364 >1k 0.0010969307693235776 0.8742992292294997 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2501 0.11421217800477125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 4.566660456008447E-5 6 0.0 0.0 0.0 0.0 9.133320912016894E-5 7 0.0 0.0 0.0 4.566660456008447E-5 9.133320912016894E-5 8 0.0 0.0 0.0 4.566660456008447E-5 9.133320912016894E-5 9 0.0 0.0 0.0 4.566660456008447E-5 9.133320912016894E-5 10-11 0.0 0.0 0.0 6.84999068401267E-5 9.133320912016894E-5 12-13 0.0 0.0 0.0 9.133320912016894E-5 4.109994410407602E-4 14-15 0.0 0.0 0.0 9.133320912016894E-5 5.479992547210136E-4 16-17 0.0 0.0 0.0 9.133320912016894E-5 5.93665859281098E-4 18-19 6.84999068401267E-5 0.0 0.0 1.1416651140021116E-4 5.93665859281098E-4 20-21 1.369998136802534E-4 0.0 0.0 1.5983311596029563E-4 7.534989752413937E-4 22-23 1.8266641824033787E-4 0.0 0.0 2.96832929640549E-4 8.90498788921647E-4 24-25 3.1966623192059126E-4 0.0 0.0 6.164991615611403E-4 0.0010503319048819426 26-27 3.1966623192059126E-4 0.0 0.0 0.0015983311596029562 0.0010503319048819426 28-29 4.1099944104076023E-4 0.0 0.0 0.006005158499651107 0.0010503319048819426 30-31 5.023326501609291E-4 0.0 0.0 0.024637133160165567 0.0010731652071619849 32-33 5.023326501609291E-4 0.0 0.0 0.06468674535935964 0.0011188318117220694 34-35 5.479992547210136E-4 0.0 0.0 0.1298758233688802 0.0011644984162821539 36-37 6.393324638411825E-4 0.0 0.0 0.24095983896128567 0.001187331718562196 38-39 6.393324638411825E-4 0.0 0.0 0.4164566002856903 0.001187331718562196 40-41 6.393324638411825E-4 0.0 0.0 0.6052423435370795 0.0012101650208422383 42-43 6.393324638411825E-4 0.0 0.0 0.7954665848321112 0.0012329983231222806 44-45 6.393324638411825E-4 0.0 0.0 1.022977608750452 0.0012329983231222806 46-47 6.84999068401267E-4 2.2833302280042234E-5 0.0 1.2852409187390172 0.0012558316254023228 48-49 6.84999068401267E-4 9.133320912016894E-5 0.0 1.5418187364598517 0.001278664927682365 50-51 6.84999068401267E-4 9.133320912016894E-5 0.0 1.7985563872966468 0.0013243315322424495 52-53 6.84999068401267E-4 9.133320912016894E-5 0.0 2.0436947205751803 0.0013471648345224918 54-55 6.84999068401267E-4 9.133320912016894E-5 0.0 2.3029897012673395 0.001369998136802534 56-57 6.84999068401267E-4 9.133320912016894E-5 0.0 2.529678726303599 0.001369998136802534 58-59 6.84999068401267E-4 9.133320912016894E-5 0.0 2.6867946792925697 0.0013928314390825763 60-61 6.84999068401267E-4 9.133320912016894E-5 0.0 2.8007785242745404 0.0014156647413626185 62-63 6.84999068401267E-4 9.133320912016894E-5 0.0 2.8993042236129227 0.0014156647413626185 64-65 6.84999068401267E-4 9.133320912016894E-5 0.0 3.0025107499187134 0.0014156647413626185 66-67 7.306656729613515E-4 9.133320912016894E-5 0.0 3.08715380147083 0.0014156647413626185 68-69 7.306656729613515E-4 9.133320912016894E-5 0.0 3.185474001088692 0.0014156647413626185 70-71 7.306656729613515E-4 9.133320912016894E-5 0.0 3.36839158565411 0.0014156647413626185 72-73 7.306656729613515E-4 9.133320912016894E-5 0.0 3.5956057766428104 0.0014156647413626185 74-75 7.534989752413937E-4 9.133320912016894E-5 0.0 3.8581430862587363 0.001461331345922703 76-77 7.763322775214359E-4 9.133320912016894E-5 0.0 4.241788231168005 0.0015069979504827872 78-79 7.763322775214359E-4 9.133320912016894E-5 0.0 4.787800988590655 0.0015069979504827872 80-81 8.676654866416048E-4 9.133320912016894E-5 0.0 5.400281488950508 0.0015983311596029562 82-83 9.133320912016893E-4 9.133320912016894E-5 0.0 5.977575870496816 0.0017353309732832097 84-85 9.589986957617737E-4 9.133320912016894E-5 0.0 6.4393337425061095 0.0017353309732832097 86-87 0.0010046653003218582 9.133320912016894E-5 0.0 6.847867186900626 0.001758164275563252 88-89 0.0010046653003218582 9.133320912016894E-5 0.0 7.265396952393479 0.0017809975778432941 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2940 0.0 40.10572 1 GTATCAA 4795 0.0 35.803577 1 ATCAACG 5780 0.0 29.042671 3 TCAACGC 5790 0.0 28.992512 4 CAACGCA 5930 0.0 28.308035 5 TATCAAC 6080 0.0 27.609644 2 AACGCAG 6340 0.0 26.458647 6 TACATGG 7120 0.0 23.24564 2 GTACATG 7305 0.0 23.243198 1 ACGCAGA 7385 0.0 22.714666 7 CGCAGAG 7480 0.0 22.42618 8 ACATGGG 7185 0.0 21.9853 3 GCAGAGT 8645 0.0 18.967981 9 GAGTACA 5665 0.0 18.732523 1 AGAGTAC 7050 0.0 18.647558 10-11 CATGGGG 4455 0.0 18.522718 4 TAAGGTG 1115 0.0 17.761875 5 GAGTACT 3955 0.0 17.692404 12-13 AGTACAT 5780 0.0 16.72401 2 CAGAGTA 8095 0.0 16.560457 10-11 >>END_MODULE