FastQCFastQC Report
Fri 27 May 2016
SRR939806_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939806_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2273691
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23750.1044557066021724No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22740.10001359023719583No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA56200.035.6494751
GGTATCA35300.034.7732771
ATCAACG64950.030.5591873
TCAACGC66350.029.7017214
CAACGCA68300.028.9927235
TATCAAC68150.028.8469432
AACGCAG71250.027.8589556
GTACATG78150.026.719841
TACATGG76150.026.6193072
ACATGGG80150.024.4703983
ACGCAGA83950.023.4763497
CGCAGAG84200.023.0161088
GTAAGGT9450.022.3969674
TAAGGTG10250.021.567595
GCAGAGT90800.019.7894559
CATGGGG47900.019.638264
GAGTACT38450.019.2074312-13
TAGCCCT15950.018.8724294
GTAGCCC14500.018.8134543
AGAGTAC73400.018.68094410-11
Produced by FastQC (version 0.11.5)