FastQCFastQC Report
Fri 27 May 2016
SRR939806_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939806_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2273691
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26300.11567095088998462No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35150.037.5337331
GTATCAA53650.033.7906041
GTACATG86450.027.5026821
ATCAACG64750.027.326873
TCAACGC65750.026.9112514
TATCAAC66100.026.8452432
CAACGCA66100.026.7687555
TACATGG86150.026.6555272
AACGCAG70050.025.462476
ACATGGG87500.024.9385133
TATACCG1151.2938255E-424.7499435
GGACCGT2602.5465852E-1123.7186976
CGTAGAC1503.0659587E-522.1374473
ACGCAGA81400.021.9121117
CGCAGAG82550.021.549398
CATGGGA36800.020.6249524
GAGTACA62050.020.4972361
CATGGGG50900.020.1306024
TAAGGTG10950.019.4948185
GGACCGA4950.019.1666226
Produced by FastQC (version 0.11.5)