##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939806_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2273691 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.375562026678207 31.0 31.0 34.0 30.0 34.0 2 31.604939721360555 31.0 31.0 34.0 30.0 34.0 3 31.661262678173948 31.0 31.0 34.0 30.0 34.0 4 35.46999174470058 37.0 35.0 37.0 33.0 37.0 5 35.20072912282276 37.0 35.0 37.0 32.0 37.0 6 35.217259073462486 37.0 35.0 37.0 32.0 37.0 7 35.115922524212834 37.0 35.0 37.0 32.0 37.0 8 35.07143187002983 37.0 35.0 37.0 32.0 37.0 9 36.664682228147974 39.0 35.0 39.0 32.0 39.0 10-11 36.51607276450494 38.0 35.0 39.0 32.0 39.0 12-13 36.43866426880346 38.0 35.0 39.0 32.0 39.0 14-15 37.503395580138196 39.0 36.0 41.0 32.0 41.0 16-17 37.41290417211486 39.0 36.0 41.0 32.0 41.0 18-19 37.3543386062574 39.0 36.0 41.0 32.0 41.0 20-21 37.24583573581458 39.0 36.0 40.0 31.0 41.0 22-23 37.1228071448583 39.0 36.0 40.0 31.0 41.0 24-25 36.975531635565254 39.0 36.0 40.0 31.0 41.0 26-27 36.67999191622785 38.5 35.5 40.0 30.0 41.0 28-29 36.45857308666832 38.0 35.0 40.0 30.0 41.0 30-31 36.21383534526019 38.0 35.0 40.0 30.0 41.0 32-33 36.23336482398004 38.0 35.0 40.0 30.0 41.0 34-35 36.102396279881475 38.0 35.0 40.0 29.5 41.0 36-37 36.19507356100719 38.0 35.0 40.0 30.0 41.0 38-39 36.11173615939897 38.0 35.0 40.0 30.0 41.0 40-41 36.04288533490259 38.0 35.0 40.0 29.0 41.0 42-43 35.848098532298366 38.0 34.5 40.0 28.5 41.0 44-45 35.64847070248332 38.0 34.0 40.0 28.0 41.0 46-47 35.430385219451544 38.0 34.0 40.0 27.0 41.0 48-49 35.17164161708869 38.0 34.0 40.0 27.0 41.0 50-51 34.956238336695705 38.0 33.0 40.0 26.0 41.0 52-53 34.7080522375292 38.0 33.0 40.0 26.0 41.0 54-55 34.433163301433666 37.0 33.0 40.0 25.5 41.0 56-57 34.06351456728289 37.0 32.5 40.0 24.5 41.0 58-59 33.68044580376137 36.5 32.0 39.0 23.5 41.0 60-61 33.35684312424159 36.0 32.0 39.0 23.0 40.5 62-63 33.05952040096918 36.0 31.0 39.0 23.0 40.0 64-65 32.60784820804586 35.0 31.0 39.0 22.0 40.0 66-67 31.66156263098196 35.0 30.0 38.0 17.0 40.0 68-69 29.669267063994184 34.0 26.5 37.0 9.5 39.5 70-71 29.001986417679447 33.0 25.5 36.0 9.0 39.0 72-73 29.621389625943017 33.5 27.5 36.0 10.5 39.0 74-75 29.75698962611894 34.0 28.0 36.0 12.5 38.5 76-77 29.32831308212066 32.5 28.0 35.0 13.0 37.0 78-79 29.87515915751085 34.0 29.0 35.0 13.5 37.0 80-81 29.69652120714732 34.0 29.0 35.0 12.0 37.0 82-83 29.257050760195646 33.5 29.0 35.0 7.5 36.5 84-85 28.897519935646486 33.0 29.0 35.0 7.0 36.0 86-87 28.519529918533344 33.0 27.5 35.0 2.0 35.5 88-89 28.06448171717265 33.0 27.0 35.0 2.0 35.0 90-91 27.57229412439949 33.0 26.0 35.0 2.0 35.0 92-93 27.07761542795393 32.0 25.5 34.5 2.0 35.0 94-95 26.234288872146657 32.0 24.0 34.0 2.0 35.0 96-97 25.7023727058778 32.0 23.5 34.0 2.0 35.0 98-99 25.009162634676393 31.5 20.0 34.0 2.0 35.0 100-101 23.50705065024227 30.0 9.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.32874898729255264 1101 2 0.3851801103942414 1101 3 0.4060373294787887 1101 4 0.28498110896143203 1101 5 0.24672321213279247 1101 6 0.27713167909193714 1101 7 0.20807240704886 1101 8 0.22481729930139238 1101 9 0.41929510593426045 1101 10-11 0.4321669668884809 1101 12-13 0.41042759036528764 1101 14-15 0.5486679320517922 1101 16-17 0.533448117565861 1101 18-19 0.4067212368317499 1101 20-21 0.48822145462019506 1101 22-23 0.4044042935359542 1101 24-25 0.35050704013943346 1101 26-27 0.4161015229652776 1101 28-29 0.36446302205732906 1101 30-31 0.23201597779084437 1101 32-33 0.1678916369926 1101 34-35 0.2833705158136084 1101 36-37 0.454947703010923 1101 38-39 0.5315388831218968 1101 40-41 0.4997063760125826 1101 42-43 0.30029857147541605 1101 44-45 0.23565049865541 1101 46-47 0.20523919195754559 1101 48-49 0.3984402804717959 1101 50-51 0.3609963603540862 1101 52-53 0.23309884291930416 1101 54-55 0.1457171869593452 1101 56-57 -0.004340686121430792 1101 58-59 0.09244408455948161 1101 60-61 0.06391719022646924 1101 62-63 0.30446688074502504 1101 64-65 0.11716253901174412 1101 66-67 -0.0648679445373368 1101 68-69 -8.75380154794971 1101 70-71 -6.654029256141651 1101 72-73 -1.4472969273337881 1101 74-75 -0.27973349163971406 1101 76-77 -0.20681692297404908 1101 78-79 -0.21156377490560985 1101 80-81 -0.17631972253914796 1101 82-83 -0.09341817340989422 1101 84-85 -0.03777811211227444 1101 86-87 -0.09285869964682902 1101 88-89 -0.16694984632145804 1101 90-91 -0.38841616746084284 1101 92-93 -0.721430642777694 1101 94-95 -0.24965257579666655 1101 96-97 -0.6394474655189128 1101 98-99 -0.39408570022442646 1101 100-101 -0.45463870525124506 1102 1 0.2514986281826097 1102 2 0.17085422682516693 1102 3 0.29158331796382697 1102 4 0.20402207335524736 1102 5 0.22665529328784118 1102 6 0.3159924437357944 1102 7 0.2825328111690908 1102 8 0.2853331004837827 1102 9 0.38028159457341815 1102 10-11 0.4448948543013955 1102 12-13 0.3653796354817729 1102 14-15 0.47525770471088435 1102 16-17 0.5649239404844906 1102 18-19 0.5379500617578117 1102 20-21 0.42602000893975145 1102 22-23 0.3090788775166544 1102 24-25 0.41845898595330766 1102 26-27 0.36883210327177807 1102 28-29 0.35110475273624786 1102 30-31 0.328910323265994 1102 32-33 0.13526788629179265 1102 34-35 0.07552990270276183 1102 36-37 0.26223772109917576 1102 38-39 0.3884714118282844 1102 40-41 0.2840615435064251 1102 42-43 0.23213448702130535 1102 44-45 0.09671718794100315 1102 46-47 0.17715948368653045 1102 48-49 0.3133003637894447 1102 50-51 0.3110389604824917 1102 52-53 0.22874151007696497 1102 54-55 0.1554424635412488 1102 56-57 0.14016003554646517 1102 58-59 0.1950391064646695 1102 60-61 0.29962795897345984 1102 62-63 0.255526470583888 1102 64-65 0.16937726296773548 1102 66-67 0.5521987943995583 1102 68-69 -4.419573284359217 1102 70-71 0.47674753504634637 1102 72-73 0.8312410210881644 1102 74-75 0.5481744886349276 1102 76-77 0.30349330802173924 1102 78-79 0.2116090226625822 1102 80-81 0.1795525439669774 1102 82-83 0.2860754419152549 1102 84-85 0.1780803244371505 1102 86-87 0.05456429428537035 1102 88-89 0.29445247198063385 1102 90-91 0.16471374379434778 1102 92-93 -0.16660327759994686 1102 94-95 0.1868663368673822 1102 96-97 -0.2590715051627228 1102 98-99 0.24071207070790734 1102 100-101 0.243126744751617 1103 1 0.2167352900397468 1103 2 0.25023835870569044 1103 3 0.18381261627054357 1103 4 0.18256370892583362 1103 5 0.23071851368023744 1103 6 0.14817729538678748 1103 7 0.21779285727420472 1103 8 0.23405981988938862 1103 9 0.3983981675372448 1103 10-11 0.4503687999371664 1103 12-13 0.39607042440991336 1103 14-15 0.3076229135935833 1103 16-17 0.3699391153688154 1103 18-19 0.3100645533577193 1103 20-21 0.3566591783843407 1103 22-23 0.31056941601774213 1103 24-25 0.20263460828940794 1103 26-27 0.36017806575746647 1103 28-29 0.23494018401807182 1103 30-31 0.22404606799624815 1103 32-33 0.28421290013991296 1103 34-35 0.2426484989845008 1103 36-37 0.30566857172191675 1103 38-39 0.43336688755676533 1103 40-41 0.33636721377195045 1103 42-43 0.20464555722380595 1103 44-45 0.25454324064677536 1103 46-47 0.07677513620655674 1103 48-49 0.02808820563895864 1103 50-51 0.045835053157077255 1103 52-53 0.06692997716487525 1103 54-55 0.020673113490659034 1103 56-57 0.14118413293108034 1103 58-59 0.06535707419109116 1103 60-61 0.2139251047684425 1103 62-63 0.08763772819205684 1103 64-65 -0.029214706781999666 1103 66-67 0.28253985614392363 1103 68-69 -3.7664023139958474 1103 70-71 0.6005285689286275 1103 72-73 0.7746979924134365 1103 74-75 0.4945835139595687 1103 76-77 0.23447246121344278 1103 78-79 0.1452510363614401 1103 80-81 0.20434129796800704 1103 82-83 0.08993280629491096 1103 84-85 0.0673731167817131 1103 86-87 0.030136254747745284 1103 88-89 0.09441660784145611 1103 90-91 0.008225858824946641 1103 92-93 0.03965905717624452 1103 94-95 -0.24862589326015438 1103 96-97 -0.7344206104250048 1103 98-99 -0.3903578830211316 1103 100-101 -0.5201440232799612 1104 1 0.20052399641242147 1104 2 0.14009185788799527 1104 3 0.22178252392037834 1104 4 0.25011809455154577 1104 5 0.26267653047459305 1104 6 0.22627548356199156 1104 7 0.1771278367444893 1104 8 0.22638668683157448 1104 9 0.3354847871023807 1104 10-11 0.23655947657196208 1104 12-13 0.20402005073950846 1104 14-15 0.3132326690361751 1104 16-17 0.2549720246809031 1104 18-19 0.2875249977341383 1104 20-21 0.3239254229095181 1104 22-23 0.3257807418696146 1104 24-25 0.24186916132160263 1104 26-27 0.30033222661700165 1104 28-29 0.256890802594981 1104 30-31 0.1963571314866428 1104 32-33 0.17688923551144597 1104 34-35 0.14082331443187712 1104 36-37 0.25508812718438634 1104 38-39 0.26828409062775194 1104 40-41 0.13889187225895228 1104 42-43 0.11795717927291349 1104 44-45 0.14597725284376395 1104 46-47 0.06569560066959923 1104 48-49 0.06298620825451451 1104 50-51 0.0926952416169371 1104 52-53 0.044612804438656894 1104 54-55 0.165944867069598 1104 56-57 0.041646890760965505 1104 58-59 -0.023617064272393407 1104 60-61 -0.04238519875988089 1104 62-63 0.08210903760941335 1104 64-65 0.024667410477732687 1104 66-67 0.09193606224134498 1104 68-69 -4.045601104501426 1104 70-71 0.622260664839601 1104 72-73 0.768540755452964 1104 74-75 0.6268256437868729 1104 76-77 0.4669114292762373 1104 78-79 0.03850506375767182 1104 80-81 0.0037903590254551034 1104 82-83 0.03881612526146938 1104 84-85 0.03344151471182144 1104 86-87 0.16450067267855673 1104 88-89 0.0891920363607106 1104 90-91 0.021848447690832273 1104 92-93 -0.3967407768673681 1104 94-95 0.2218957012101157 1104 96-97 -0.30610880039941435 1104 98-99 -0.042625215124566296 1104 100-101 0.01413768499664414 1105 1 0.15424405845682898 1105 2 0.2646194140212934 1105 3 0.21428856935491325 1105 4 0.0978115946306275 1105 5 0.2585455535034882 1105 6 0.20692841399844042 1105 7 0.21833854225954497 1105 8 0.2461481469639466 1105 9 0.1280920459221946 1105 10-11 0.24667191611341366 1105 12-13 0.20945271445484082 1105 14-15 0.19218763915404935 1105 16-17 0.2582046237190738 1105 18-19 0.13967420386053675 1105 20-21 0.2155477653445601 1105 22-23 0.22696026063253782 1105 24-25 0.20187669355860294 1105 26-27 0.26712498465489176 1105 28-29 0.2539982959786684 1105 30-31 0.31873373527721327 1105 32-33 0.2623428504646981 1105 34-35 0.24814467901934734 1105 36-37 0.11911479459431717 1105 38-39 0.43894181308480285 1105 40-41 0.37283020798717814 1105 42-43 0.3846008876722422 1105 44-45 0.32114583827756604 1105 46-47 0.36236826939524036 1105 48-49 0.3843337533262101 1105 50-51 0.3485567357669481 1105 52-53 0.2857806437007895 1105 54-55 0.26002663767923195 1105 56-57 0.40787664514557065 1105 58-59 0.4909621630738954 1105 60-61 0.3689287551541156 1105 62-63 0.3398723124635268 1105 64-65 0.29253103568328953 1105 66-67 0.8753878584431192 1105 68-69 2.2635104172690212 1105 70-71 2.569023901747091 1105 72-73 1.5043493056669242 1105 74-75 0.9932476498737799 1105 76-77 0.6744552853315966 1105 78-79 0.6603317773080057 1105 80-81 0.6269269866854543 1105 82-83 0.7219476250900385 1105 84-85 0.5314599789347056 1105 86-87 0.569571231571885 1105 88-89 0.6991098903145669 1105 90-91 0.7973668808512109 1105 92-93 0.593161895078687 1105 94-95 0.8867429727638836 1105 96-97 0.46799872354305805 1105 98-99 0.7004844502835965 1105 100-101 0.5840563549708229 1106 1 0.41864043405282203 1106 2 0.3991840216510809 1106 3 0.434909559445817 1106 4 0.3708604726146021 1106 5 0.36425983504000925 1106 6 0.3770335512515217 1106 7 0.397812486783657 1106 8 0.38647024472283675 1106 9 0.5015923111322991 1106 10-11 0.48511051037925057 1106 12-13 0.48752212383140403 1106 14-15 0.6383823402891267 1106 16-17 0.5999551639178691 1106 18-19 0.5932507384509904 1106 20-21 0.47621173372844083 1106 22-23 0.48014104545703873 1106 24-25 0.7064667840224814 1106 26-27 0.6570672708427239 1106 28-29 0.5717496067672485 1106 30-31 0.5258991053814697 1106 32-33 0.6088242197749807 1106 34-35 0.7679640619845642 1106 36-37 0.6368080187047056 1106 38-39 0.8188121470960041 1106 40-41 0.5604914862523316 1106 42-43 0.5825344422150422 1106 44-45 0.729859278672194 1106 46-47 0.7015310462528319 1106 48-49 0.6212554774148558 1106 50-51 0.7766188157671792 1106 52-53 0.7461493716463963 1106 54-55 0.6284214713645326 1106 56-57 0.5559444862914802 1106 58-59 0.6572647979572679 1106 60-61 0.7204610883047948 1106 62-63 0.7073410127366273 1106 64-65 0.7380257905183925 1106 66-67 1.3058515429370487 1106 68-69 2.767450185514509 1106 70-71 3.0067534473952158 1106 72-73 1.7254915770863413 1106 74-75 1.3332422469719312 1106 76-77 0.9025466645593738 1106 78-79 0.8625873791631271 1106 80-81 0.8930350732357013 1106 82-83 1.070772658437786 1106 84-85 1.1573153317738054 1106 86-87 0.8722343540616606 1106 88-89 0.9788376396257661 1106 90-91 0.9886171774076438 1106 92-93 0.8106087717225456 1106 94-95 1.2196179306793695 1106 96-97 1.1775367279129014 1106 98-99 1.429137961603189 1106 100-101 1.5906919994277118 1107 1 0.14635386178236942 1107 2 0.12619716268961056 1107 3 0.17087103785304691 1107 4 0.1220024077808759 1107 5 0.17236869269898847 1107 6 0.13999543137942538 1107 7 0.07463929181159301 1107 8 0.21684773062785467 1107 9 0.11338922010028796 1107 10-11 0.22153731210021732 1107 12-13 0.12572436933346864 1107 14-15 0.1823034872937228 1107 16-17 0.04236630717581846 1107 18-19 0.16439644655977048 1107 20-21 0.1387262533565803 1107 22-23 0.10111082696834472 1107 24-25 0.19858682071472344 1107 26-27 0.2461219608470202 1107 28-29 0.29642213301908527 1107 30-31 0.3724086450261126 1107 32-33 0.3504387235071462 1107 34-35 0.3131853522031278 1107 36-37 0.30086299994709265 1107 38-39 0.3529846156638925 1107 40-41 0.30833961253918574 1107 42-43 0.2142460240587738 1107 44-45 0.2315616122972628 1107 46-47 0.2549570772262797 1107 48-49 0.3400349417847863 1107 50-51 0.33015438984254786 1107 52-53 0.47801867300943 1107 54-55 0.4412885018220294 1107 56-57 0.47259233208786355 1107 58-59 0.44896154356887763 1107 60-61 0.6057145011454992 1107 62-63 0.5766129701159102 1107 64-65 0.5836514695259183 1107 66-67 0.9995689360141498 1107 68-69 2.390179233000598 1107 70-71 2.7042624785945186 1107 72-73 1.7251960173962466 1107 74-75 0.9640932672259446 1107 76-77 0.7801880531375076 1107 78-79 0.8538732535895264 1107 80-81 0.757367726512431 1107 82-83 0.8822186348989831 1107 84-85 0.7446430738368157 1107 86-87 0.7886910161424261 1107 88-89 0.8402851568304222 1107 90-91 0.7330636427730504 1107 92-93 0.5804754372868892 1107 94-95 1.0339015056401095 1107 96-97 0.7628034437580951 1107 98-99 0.9704546884778509 1107 100-101 1.1226194920405987 1108 1 -0.1336067790508828 1108 2 -0.1502383750141938 1108 3 -0.03912572407512016 1108 4 -0.07405701128571707 1108 5 -0.14430468631418591 1108 6 0.006479055795388433 1108 7 -0.053239071500065904 1108 8 -0.004793891063101796 1108 9 -0.05663448689098516 1108 10-11 0.08106841059590408 1108 12-13 -0.012516451719562838 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64-65 -0.22594946646486846 2316 66-67 0.2790237717768669 2316 68-69 1.6441781837090979 2316 70-71 1.9875823605146827 2316 72-73 1.0568169370665252 2316 74-75 0.7708936880804274 2316 76-77 0.17189422063076165 2316 78-79 0.03972108681476172 2316 80-81 -0.12164453337374681 2316 82-83 -0.12272983927999093 2316 84-85 -0.2163390906459277 2316 86-87 -0.19801565527084364 2316 88-89 -0.25796238005733585 2316 90-91 -0.13339196675071463 2316 92-93 0.060089332962444786 2316 94-95 0.2494987036822991 2316 96-97 0.24893548380510566 2316 98-99 0.13276813647736674 2316 100-101 0.030356716449183807 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 18.0 4 25.0 5 106.0 6 214.0 7 492.0 8 1193.0 9 2027.0 10 3191.0 11 4707.0 12 6810.0 13 8512.0 14 10260.0 15 12236.0 16 14592.0 17 16893.0 18 16883.0 19 16468.0 20 17022.0 21 17857.0 22 19953.0 23 22880.0 24 27794.0 25 34267.0 26 42058.0 27 52641.0 28 64118.0 29 78168.0 30 93820.0 31 110682.0 32 131972.0 33 160251.0 34 197572.0 35 249208.0 36 309950.0 37 331201.0 38 182879.0 39 14767.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.49113576575546 19.203230716441286 14.053660510826244 24.25197300697701 2 16.235622450137736 20.27981287739281 38.82543347108565 24.6591312013838 3 19.750370433247465 23.62961689131627 29.601409867400513 27.01860280803575 4 13.532181813623751 16.294386528336524 36.117176872319064 34.05625478572066 5 13.87286135187235 37.40165220339967 34.38413575107611 14.341350693651863 6 33.62383053435652 35.7681880715384 16.719708244930704 13.888273149174383 7 29.492046192732435 31.837307708039482 20.248441850717622 18.42220424851046 8 26.735207202737747 35.705159584129945 19.06859815163978 18.491035061492525 9 26.361585633228085 16.09545888161584 19.373784740318715 38.16917074483736 10-11 25.582258099275585 26.00707396035785 27.292187021015607 21.118480919350958 12-13 26.009800804067044 22.99993710666929 27.646764665911068 23.343497423352602 14-15 22.760062822960553 24.824261520144997 25.21129740144989 27.20437825544456 16-17 22.95384249999725 27.934639071198376 26.021113326069052 23.09040510273532 18-19 22.985731130571395 26.83427519394676 27.55985751801806 22.62013615746379 20-21 23.62926624594107 26.044875930810303 27.57971509761001 22.74614272563862 22-23 23.266200200466994 26.024732472442384 27.436269924101385 23.272797402989237 24-25 23.143903334206364 26.274036098596575 27.51343136342935 23.06862920376771 26-27 22.94496508749677 26.50333115771585 27.373636793855262 23.178066960932124 28-29 22.965947834531384 26.751515599325852 27.319711411592092 22.962825154550668 30-31 23.146124591686704 26.352442270976212 27.213402220098402 23.288030917238682 32-33 22.87213642253266 26.39514048385806 27.234982464257783 23.497740629351497 34-35 23.417676283083903 26.489903982438378 27.122844465009045 22.969575269468674 36-37 23.504887252638007 26.157159040327706 27.20069457499768 23.13725913203661 38-39 23.073978583901365 26.441135884831073 27.218150173309407 23.266735357958158 40-41 23.39947875287255 26.240119298185366 27.222845584886258 23.13755636405583 42-43 23.154706148466214 26.183138635755743 27.153328057254626 23.508827158523417 44-45 23.07492668641067 26.33081609768747 27.379879519610533 23.21437769629133 46-47 23.28683148595997 26.46610245784997 26.946279393640975 23.300786662549083 48-49 23.062569845437014 26.510438873122492 26.99880939462262 23.42818188681787 50-51 23.05266135236299 26.60368450073023 27.066871960352934 23.276782186553852 52-53 23.358340029000992 26.33038019728507 26.938899199226757 23.372380574487185 54-55 22.955011349923556 26.39462350732442 27.06978772694346 23.580577415808566 56-57 22.847929415952112 26.563851853220623 27.033885684129434 23.554333046697828 58-59 22.97256519998768 26.254426209317362 26.95855088656148 23.814457704133474 60-61 22.74150311122078 26.439251635554463 27.16202518227138 23.657220070953382 62-63 22.826074287665847 26.340007595767922 27.207825180344507 23.626092936221717 64-65 22.506545892944246 26.43887589655532 27.068020547484277 23.986557663016157 66-67 22.352500133258797 26.5427514042834 27.195014667278116 23.90973379517969 68-69 22.320228394431847 26.14783618539246 27.670318486193366 23.86161693398233 70-71 22.222771795161115 26.194689991916125 27.305102114945583 24.277436097977176 72-73 22.307571298327776 26.418001800497322 27.107773821227273 24.166653079947633 74-75 22.56621653428126 26.70368032168444 26.545829694659346 24.184273449374956 76-77 22.57967086753089 26.429148087613207 26.698556278308537 24.292624766547366 78-79 22.70758510973555 26.580552824301947 26.505582936510468 24.20627912945204 80-81 22.746437849257685 26.621970772694176 26.324499051419924 24.307092326628215 82-83 22.672918763569623 26.693782641041075 26.41014685156137 24.22315174382793 84-85 22.619317791980915 26.637546422621337 26.240084339193448 24.503051446204296 86-87 22.58813486006376 26.62846981772345 26.294734855973438 24.488660466239352 88-89 22.55218066514872 26.649028648182444 26.115555923044813 24.683234763624018 90-91 22.443137211782688 26.726489054064455 26.196729963605176 24.633643770547682 92-93 22.48731540483387 26.487734999278683 26.279059334349043 24.74589026153841 94-95 22.105083383475957 26.374681812599672 26.581319301909055 24.938915502015316 96-97 22.39337868831835 26.047825960728904 26.524308436185574 25.03448691476717 98-99 22.285314739455057 26.211887337004075 26.290121862865895 25.212676060674976 100-101 22.073890246458436 26.226579542737927 26.42260178454356 25.276928426260074 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 76.0 1 56.0 2 42.0 3 54.5 4 61.0 5 66.5 6 101.5 7 198.5 8 314.0 9 385.5 10 401.5 11 414.0 12 460.5 13 535.5 14 642.5 15 745.5 16 840.5 17 956.0 18 1108.0 19 1338.0 20 1565.0 21 1848.5 22 2295.5 23 3024.5 24 3935.0 25 5210.5 26 7451.5 27 10314.0 28 13201.5 29 16654.5 30 20428.0 31 25194.5 32 31149.0 33 34908.0 34 37767.5 35 41848.0 36 47321.0 37 52549.5 38 56319.5 39 59964.0 40 63902.5 41 67572.5 42 70130.5 43 73041.0 44 76768.0 45 81031.0 46 84549.5 47 87876.0 48 91093.5 49 91215.5 50 90100.0 51 89911.5 52 90173.0 53 90630.0 54 89387.5 55 84321.0 56 77393.0 57 70652.0 58 64705.0 59 57361.5 60 47489.5 61 38367.0 62 30231.5 63 22498.0 64 16430.0 65 12216.0 66 8859.5 67 6614.5 68 5021.5 69 3649.5 70 2645.0 71 1842.5 72 1270.5 73 945.0 74 708.0 75 517.0 76 358.5 77 214.0 78 125.5 79 68.0 80 40.0 81 25.0 82 13.0 83 7.5 84 5.0 85 2.5 86 2.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022474469925772674 2 0.023266134228441773 3 8.796270029656624E-5 4 0.0 5 0.0 6 1.3194405044484936E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.738414762604065E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 6.597202522242468E-5 26-27 3.0786945103798187E-4 28-29 1.3194405044484936E-4 30-31 3.5185080118626497E-4 32-33 0.0021550861572658727 34-35 0.004244200289309321 36-37 0.10441172525202412 38-39 0.09568142724758993 40-41 0.10588510048199161 42-43 0.10702861558584698 44-45 0.10045340373867864 46-47 0.09379022919121376 48-49 0.09488976294492084 50-51 0.09449393079358628 52-53 0.07474630457700716 54-55 0.06284934936189658 56-57 0.03384364893910386 58-59 0.01323838639463322 60-61 0.009961775808586128 62-63 0.0024849462833779964 64-65 0.0056296128189802395 66-67 0.1614995177444956 68-69 0.43488759026622353 70-71 0.4093124351549969 72-73 0.18991146994028651 74-75 0.02885176569727373 76-77 0.032810087210619204 78-79 0.004903920541533568 80-81 0.0012974498293743522 82-83 2.199067507414156E-5 84-85 6.597202522242468E-4 86-87 0.0013414311795226352 88-89 1.7592540059313248E-4 90-91 7.037016023725299E-4 92-93 0.002946750459934969 94-95 0.08083772157254437 96-97 0.01605319280412334 98-99 0.017504577359016685 100-101 0.009873813108289562 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2273691.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.06228501306843 #Duplication Level Percentage of deduplicated Percentage of total 1 74.0893032482753 40.054370286279166 2 14.145149735371612 15.294382330923792 3 4.972956317939083 8.065481454538864 4 2.322476684039309 5.022335857149569 5 1.2207103796050696 3.2997196230310113 6 0.7329533114064778 2.3775078493517583 7 0.477798972422241 1.8081632958229694 8 0.35795962733915376 1.5481692317104876 9 0.2501842501393262 1.2172979013131755 >10 1.2953374757131952 12.7039326997153 >50 0.09431198992597022 3.483806242443731 >100 0.037687309137200455 3.500652079293773 >500 0.0021950990903262637 0.8107500193184535 >1k 9.755995957003698E-4 0.8134311291079022 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2630 0.11567095088998462 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 8.796270029656624E-5 7 4.398135014828312E-5 0.0 0.0 0.0 1.3194405044484936E-4 8 4.398135014828312E-5 0.0 0.0 0.0 1.3194405044484936E-4 9 4.398135014828312E-5 0.0 0.0 0.0 1.7592540059313248E-4 10-11 8.796270029656624E-5 0.0 0.0 0.0 1.7592540059313248E-4 12-13 8.796270029656624E-5 0.0 0.0 0.0 1.7592540059313248E-4 14-15 8.796270029656624E-5 0.0 0.0 4.398135014828312E-5 2.638881008896987E-4 16-17 8.796270029656624E-5 0.0 0.0 6.597202522242468E-5 3.5185080118626497E-4 18-19 1.3194405044484936E-4 0.0 0.0 8.796270029656624E-5 3.5185080118626497E-4 20-21 1.7592540059313248E-4 0.0 0.0 1.539347255189909E-4 3.958321513345481E-4 22-23 2.199067507414156E-4 0.0 0.0 5.057855267052559E-4 5.277762017793974E-4 24-25 2.638881008896987E-4 0.0 0.0 0.0010775430786329366 6.157389020759637E-4 26-27 2.638881008896987E-4 0.0 0.0 0.0020231421068210237 6.157389020759637E-4 28-29 3.51850801186265E-4 0.0 0.0 0.006883081298206308 6.817109272983883E-4 30-31 3.958321513345481E-4 0.0 0.0 0.025729089836745628 7.037016023725299E-4 32-33 3.958321513345481E-4 0.0 0.0 0.0692046544583235 7.037016023725299E-4 34-35 3.958321513345481E-4 0.0 0.0 0.13451695942852393 7.47682952520813E-4 36-37 3.958321513345481E-4 0.0 0.0 0.2462075981300889 7.47682952520813E-4 38-39 3.958321513345481E-4 0.0 0.0 0.41777884505854135 7.47682952520813E-4 40-41 3.958321513345481E-4 0.0 0.0 0.6059310609928966 7.47682952520813E-4 42-43 4.178228264086897E-4 0.0 0.0 0.7973598875132988 7.47682952520813E-4 44-45 5.277762017793974E-4 0.0 0.0 1.0234460179505482 7.916643026690962E-4 46-47 5.937482270018221E-4 0.0 0.0 1.289841055798699 8.57636327891521E-4 48-49 6.157389020759637E-4 0.0 0.0 1.5817452767328541 9.455990281880872E-4 50-51 6.157389020759637E-4 0.0 0.0 1.8882952872663876 9.675897032622287E-4 52-53 6.157389020759637E-4 0.0 0.0 2.1771208136901627 9.895803783363704E-4 54-55 6.597202522242468E-4 0.0 0.0 2.492555056953649 0.0010115710534105119 56-57 6.597202522242468E-4 0.0 0.0 2.8508491259366378 0.0010115710534105119 58-59 6.597202522242468E-4 0.0 0.0 3.215938313517536 0.0010335617284846534 60-61 6.597202522242468E-4 0.0 0.0 3.588108498472308 0.0010555524035587949 62-63 6.597202522242468E-4 0.0 0.0 3.9395854581823126 0.001099533753707078 64-65 6.597202522242468E-4 0.0 0.0 4.302849419732057 0.001099533753707078 66-67 6.597202522242468E-4 0.0 0.0 4.692150340569585 0.001099533753707078 68-69 6.597202522242468E-4 0.0 0.0 5.128555287415924 0.001099533753707078 70-71 6.597202522242468E-4 0.0 0.0 5.621102427726546 0.001099533753707078 72-73 6.597202522242468E-4 0.0 0.0 6.131527986872447 0.0011655057789295028 74-75 6.597202522242468E-4 0.0 0.0 6.637291522902628 0.0012094871290777858 76-77 6.817109272983883E-4 0.0 0.0 7.191720422871885 0.0012314778041519275 78-79 7.037016023725299E-4 0.0 0.0 7.804380630437469 0.0012314778041519275 80-81 7.47682952520813E-4 0.0 0.0 8.454820817780428 0.0012974498293743522 82-83 8.796270029656625E-4 0.0 0.0 9.101148748884523 0.001495365905041626 84-85 9.236083531139456E-4 0.0 0.0 9.722671198504987 0.001495365905041626 86-87 9.675897032622287E-4 0.0 0.0 10.351076729423657 0.0015173565801157676 88-89 9.675897032622287E-4 0.0 0.0 11.027070081202767 0.0015393472551899093 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3515 0.0 37.533733 1 GTATCAA 5365 0.0 33.790604 1 GTACATG 8645 0.0 27.502682 1 ATCAACG 6475 0.0 27.32687 3 TCAACGC 6575 0.0 26.911251 4 TATCAAC 6610 0.0 26.845243 2 CAACGCA 6610 0.0 26.768755 5 TACATGG 8615 0.0 26.655527 2 AACGCAG 7005 0.0 25.46247 6 ACATGGG 8750 0.0 24.938513 3 TATACCG 115 1.2938255E-4 24.749943 5 GGACCGT 260 2.5465852E-11 23.718697 6 CGTAGAC 150 3.0659587E-5 22.137447 3 ACGCAGA 8140 0.0 21.912111 7 CGCAGAG 8255 0.0 21.54939 8 CATGGGA 3680 0.0 20.624952 4 GAGTACA 6205 0.0 20.497236 1 CATGGGG 5090 0.0 20.130602 4 TAAGGTG 1095 0.0 19.494818 5 GGACCGA 495 0.0 19.166622 6 >>END_MODULE