FastQCFastQC Report
Fri 27 May 2016
SRR939805_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939805_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1790952
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35880.20034037763156132No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26680.14897105003372507No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32050.039.5245171
GTATCAA52100.033.3293381
ATCAACG61350.028.924423
TCAACGC62150.028.6284454
TATCAAC62150.028.6276442
CAACGCA63000.028.0915625
AACGCAG65750.027.1338756
ACGCAGA73750.024.0618697
CGCAGAG74550.023.5477568
ACATGGG48700.023.479873
TACATGG48900.023.3831842
GTACATG50850.023.3255771
GAGTACT45400.020.58587812-13
GCAGAGT80900.020.1740559
GTATAGG5300.019.6938271
GAACCGT2451.7776802E-719.3666136
GAGTACA36350.019.3170411
AGAGTAC64300.018.74104910-11
GTATAGC7850.018.7359351
GTACTTT50450.018.43353314-15
Produced by FastQC (version 0.11.5)