FastQCFastQC Report
Fri 27 May 2016
SRR939805_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939805_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1790952
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39870.2226190316658403No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33830.1888939513733478No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23420.1307684404718831No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA17960.10028186126708029No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30400.037.701111
GTATCAA50100.036.1505551
CAACGCA61150.028.9101285
TCAACGC61300.028.8393844
ATCAACG61400.028.7948273
AACGCAG63800.027.8423216
TATCAAC63650.027.7784942
CGCAGAG73050.024.1232688
ACGCAGA73400.024.0724327
TACATGG49100.023.3346162
GTACATG52550.022.0792261
ACATGGG50450.021.7744063
TAAGGTG11000.021.4285355
AGAGTAC68100.020.86051210-11
GCAGAGT83850.020.7913869
GGTAAGG10100.020.5391713
GCGTAAT650.006536766418.73821368-69
CGCGCGA650.006786213318.61702572-73
GAGTACT47500.018.5996412-13
AGTACTT50950.018.40372712-13
Produced by FastQC (version 0.11.5)