##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939805_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1790952 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.378554534124866 31.0 31.0 34.0 30.0 34.0 2 31.58523734862799 31.0 31.0 34.0 30.0 34.0 3 31.651917527661265 31.0 31.0 34.0 30.0 34.0 4 35.46580254523851 37.0 35.0 37.0 33.0 37.0 5 35.18881689738195 37.0 35.0 37.0 32.0 37.0 6 35.21390802210221 37.0 35.0 37.0 32.0 37.0 7 35.130905239224724 37.0 35.0 37.0 32.0 37.0 8 35.05600373432677 37.0 35.0 37.0 32.0 37.0 9 36.668720881408326 39.0 35.0 39.0 32.0 39.0 10-11 36.51854572316846 38.0 35.0 39.0 32.0 39.0 12-13 36.46456242266683 38.0 35.0 39.0 32.0 39.0 14-15 37.512692411633594 39.0 36.0 41.0 32.0 41.0 16-17 37.438745147831995 39.0 36.0 41.0 32.0 41.0 18-19 37.40019805109238 39.0 36.0 41.0 32.0 41.0 20-21 37.314757179421896 39.0 36.0 40.5 31.0 41.0 22-23 37.175216867900424 39.0 36.0 40.0 31.0 41.0 24-25 37.01905243691623 39.0 36.0 40.0 31.0 41.0 26-27 36.722771464561866 39.0 35.5 40.0 30.0 41.0 28-29 36.49874340574175 38.0 35.0 40.0 30.0 41.0 30-31 36.245362243097524 38.0 35.0 40.0 30.0 41.0 32-33 36.20822975713475 38.0 35.0 40.0 30.0 41.0 34-35 36.295201378931424 38.0 35.0 40.0 30.0 41.0 36-37 36.273177896448374 38.0 35.0 40.0 30.0 41.0 38-39 36.14050544068183 38.0 35.0 40.0 30.0 41.0 40-41 35.97004135230871 38.0 35.0 40.0 29.0 41.0 42-43 35.77750715820413 38.0 34.5 40.0 28.5 41.0 44-45 35.548280467594886 38.0 34.0 40.0 27.5 41.0 46-47 35.2785445952767 38.0 34.0 40.0 27.0 41.0 48-49 34.98382564133489 38.0 33.5 40.0 26.0 41.0 50-51 34.72041601338283 38.0 33.0 40.0 26.0 41.0 52-53 34.439761925501074 38.0 33.0 40.0 24.5 41.0 54-55 34.128268094287286 37.0 33.0 40.0 24.0 41.0 56-57 32.85952889859695 36.5 31.5 39.5 16.0 41.0 58-59 29.50372511379423 34.5 24.5 38.5 8.0 40.0 60-61 29.507359493721776 34.0 25.0 38.0 9.0 40.0 62-63 31.312211885075648 35.0 29.5 38.0 12.5 40.0 64-65 30.808409717290022 35.0 29.0 38.0 4.5 40.0 66-67 25.27182414715749 28.5 18.0 36.0 2.0 39.0 68-69 17.50511376072614 13.0 7.0 29.0 2.0 35.5 70-71 16.348823419053105 13.5 7.5 25.5 2.0 33.0 72-73 18.46320476484015 18.5 7.0 30.0 2.0 34.5 74-75 19.212676274964377 17.5 7.5 30.5 2.0 35.0 76-77 20.265852742005368 23.0 8.5 31.0 2.0 34.5 78-79 23.405003595852932 28.5 12.0 34.0 2.0 36.0 80-81 26.06001807976987 31.5 22.0 34.5 2.0 36.0 82-83 26.991153308408045 33.0 26.0 35.0 2.0 36.0 84-85 26.965259537944064 33.0 26.0 35.0 2.0 36.0 86-87 26.66424560792249 33.0 25.0 35.0 2.0 36.0 88-89 26.2040962013499 33.0 24.5 35.0 2.0 35.0 90-91 24.578865039375707 31.5 11.0 34.5 2.0 35.0 92-93 22.472167316600334 30.5 2.0 34.0 2.0 35.0 94-95 17.95852987684762 22.0 2.0 33.0 2.0 35.0 96-97 16.60707740910979 13.5 2.0 32.5 2.0 34.5 98-99 15.487210433333779 2.0 2.0 32.0 2.0 34.0 100-101 14.85295753320022 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2149468654588098 1101 2 0.34473584672185353 1101 3 0.45250794669972905 1101 4 0.3354787503397958 1101 5 0.38718442181567525 1101 6 0.3823029838039034 1101 7 0.2714707931819902 1101 8 0.3997557200785167 1101 9 0.2985477418020466 1101 10-11 0.45820326104001197 1101 12-13 0.5188489400522442 1101 14-15 0.6315705588712959 1101 16-17 0.5295948127281207 1101 18-19 0.6678075332299045 1101 20-21 0.49217129758420697 1101 22-23 0.5558144891385624 1101 24-25 0.7591282544533229 1101 26-27 0.7252221229567439 1101 28-29 0.6781212902327596 1101 30-31 0.6610658403227561 1101 32-33 0.3185932185763818 1101 34-35 0.8041845861787706 1101 36-37 0.5272456715405909 1101 38-39 0.47755448710791626 1101 40-41 0.19512602876272211 1101 42-43 0.1943703307502176 1101 44-45 0.01621267567435325 1101 46-47 -0.23316579429204864 1101 48-49 -0.1931139897374834 1101 50-51 0.08745647036695203 1101 52-53 0.2568179807603528 1101 54-55 0.3412578720999164 1101 56-57 0.9981109255847471 1101 58-59 -8.920896768199828 1101 60-61 -10.128610232419508 1101 62-63 -2.7035513181844664 1101 64-65 -0.28987713172596585 1101 66-67 -1.3139346892200159 1101 68-69 -4.082946174530486 1101 70-71 -4.38492232170497 1101 72-73 -6.923987835261283 1101 74-75 -7.853960673657429 1101 76-77 -9.04648253144437 1101 78-79 -12.051400273093478 1101 80-81 -8.025342606266566 1101 82-83 -3.932470351912695 1101 84-85 -2.9679117347279735 1101 86-87 -2.734350148432437 1101 88-89 -2.970223673904343 1101 90-91 -8.472110026971324 1101 92-93 -14.447992969527933 1101 94-95 -12.882199811934615 1101 96-97 -12.50165493389415 1101 98-99 -11.85455635310218 1101 100-101 -11.729476100745382 1102 1 0.510831926949745 1102 2 0.5557575871193556 1102 3 0.5617217515607216 1102 4 0.41437876199685064 1102 5 0.5677630486152268 1102 6 0.4713337001295983 1102 7 0.5004462113786445 1102 8 0.5724476242575705 1102 9 0.779936533548856 1102 10-11 0.7030787127506954 1102 12-13 0.7414703555094988 1102 14-15 0.7881147172323111 1102 16-17 1.0103105920018791 1102 18-19 0.9027216717739392 1102 20-21 0.8981216749811907 1102 22-23 0.9373387940869833 1102 24-25 0.8942125130649714 1102 26-27 0.971034692170484 1102 28-29 1.0103599537520225 1102 30-31 1.0940450907744648 1102 32-33 0.9118814901273495 1102 34-35 0.9085670904044463 1102 36-37 0.9934711917364254 1102 38-39 0.7875482214437497 1102 40-41 0.8363256848797604 1102 42-43 0.8078635478044802 1102 44-45 0.8791175760065713 1102 46-47 0.7946681437507337 1102 48-49 0.9345170427856786 1102 50-51 1.0102658557490045 1102 52-53 0.9611050428924273 1102 54-55 0.6260514330371407 1102 56-57 1.4143167745093805 1102 58-59 -8.999779476423878 1102 60-61 -9.779823112002184 1102 62-63 -2.520261516645739 1102 64-65 0.46697138491447276 1102 66-67 -0.7082644452962512 1102 68-69 -2.999178433958125 1102 70-71 -3.722035999795544 1102 72-73 -6.477426300610702 1102 74-75 -7.0778853108317605 1102 76-77 -8.464535294165126 1102 78-79 -11.522407005039621 1102 80-81 -7.376365512369034 1102 82-83 -3.0035073193312414 1102 84-85 -1.9743376833203854 1102 86-87 -1.6614717314597733 1102 88-89 -1.7194568586102967 1102 90-91 -8.167924781881855 1102 92-93 -14.8834092719118 1102 94-95 -12.50993284364179 1102 96-97 -12.156456909764058 1102 98-99 -11.743542080502074 1102 100-101 -11.445485783382207 1103 1 0.23875638926833176 1103 2 0.26837530250416464 1103 3 0.17814570180177114 1103 4 0.1870943965983045 1103 5 0.2643102721558108 1103 6 0.24131658924607535 1103 7 0.38724460270579897 1103 8 0.35536796497648027 1103 9 0.3130885581285767 1103 10-11 0.39925343110804334 1103 12-13 0.3977392461747016 1103 14-15 0.3582499806400037 1103 16-17 0.40270280592539365 1103 18-19 0.41459749921629907 1103 20-21 0.37913983499917236 1103 22-23 0.39076346873040535 1103 24-25 0.4796001932288405 1103 26-27 0.3336830073104906 1103 28-29 0.5183848956749344 1103 30-31 0.3010318267173133 1103 32-33 0.4215906675559751 1103 34-35 0.4581599263148206 1103 36-37 0.26461812052018274 1103 38-39 0.12840482724396196 1103 40-41 0.2228471171980999 1103 42-43 0.3759817253080442 1103 44-45 0.21053665526618204 1103 46-47 0.1602228111501276 1103 48-49 0.1508273367931281 1103 50-51 0.13241820506083002 1103 52-53 0.13613345695083012 1103 54-55 0.1362196067937944 1103 56-57 0.7523201092582141 1103 58-59 -6.500467346431119 1103 60-61 -8.523614484120188 1103 62-63 -2.5797630528783486 1103 64-65 -0.21122066914093196 1103 66-67 -1.6701209137098125 1103 68-69 -4.150824575891029 1103 70-71 -4.931542219664154 1103 72-73 -7.8916323930844126 1103 74-75 -8.360848428759471 1103 76-77 -9.216252939607635 1103 78-79 -12.52640027309348 1103 80-81 -8.981209953205344 1103 82-83 -5.069162528783444 1103 84-85 -4.0964831632994 1103 86-87 -3.909010012378019 1103 88-89 -4.088123333768294 1103 90-91 -9.52470356438629 1103 92-93 -14.855305894698 1103 94-95 -12.512535696288356 1103 96-97 -12.302420240016598 1103 98-99 -11.938548700040954 1103 100-101 -11.675203141561708 1104 1 0.03467475661527075 1104 2 0.08419162903477684 1104 3 0.13579876302626204 1104 4 0.06362500884319644 1104 5 -0.03926115641561978 1104 6 -0.011234431162087333 1104 7 0.0852037863792674 1104 8 0.15995980171117452 1104 9 -0.04609511534081179 1104 10-11 0.08904934947538834 1104 12-13 0.11074945025632843 1104 14-15 0.021005082680822795 1104 16-17 0.014947703884580221 1104 18-19 0.1505668869713972 1104 20-21 0.018935753366527308 1104 22-23 0.07520224424060729 1104 24-25 0.033426723841081696 1104 26-27 0.08011157873906427 1104 28-29 -0.09079877779445411 1104 30-31 -0.045141642670436966 1104 32-33 -0.009531781423618213 1104 34-35 -0.07780946144028178 1104 36-37 -0.11701453254104166 1104 38-39 -0.16827884622542655 1104 40-41 -0.013377372597815906 1104 42-43 -0.10105909101849164 1104 44-45 -0.19099395697871557 1104 46-47 -0.22421596436007007 1104 48-49 -0.16983592851300244 1104 50-51 -0.26324506024529626 1104 52-53 -0.2656522573348852 1104 54-55 -0.4709232503490668 1104 56-57 0.18701398680922665 1104 58-59 -6.971385713778059 1104 60-61 -8.546063463712024 1104 62-63 -2.927467134510998 1104 64-65 -0.6352002609776655 1104 66-67 -2.368335199424095 1104 68-69 -4.895212330993072 1104 70-71 -5.194297321704969 1104 72-73 -8.034999740023189 1104 74-75 -8.964419857330899 1104 76-77 -9.72773253144437 1104 78-79 -12.508032926154701 1104 80-81 -9.160036483817585 1104 82-83 -5.688550283885483 1104 84-85 -4.860768877585116 1104 86-87 -4.647275318500466 1104 88-89 -4.635062109278493 1104 90-91 -9.642050503161798 1104 92-93 -15.206581404902083 1104 94-95 -12.607688757512847 1104 96-97 -12.462369219608437 1104 98-99 -11.796967067387893 1104 100-101 -11.329539876255586 1105 1 0.05227906836964635 1105 2 0.17455074003823157 1105 3 0.17230182122283466 1105 4 0.13243897359824075 1105 5 0.1924956412589367 1105 6 0.29144527573551215 1105 7 0.20699873581304473 1105 8 0.24639624572498064 1105 9 0.18166028949174517 1105 10-11 0.34289707550942694 1105 12-13 0.18721344831846665 1105 14-15 0.28461254346928655 1105 16-17 0.23150814111585305 1105 18-19 0.26080200475738735 1105 20-21 0.25392636682258996 1105 22-23 0.2995565408553986 1105 24-25 0.16902519171487285 1105 26-27 0.2531373765952978 1105 28-29 0.24279823667898626 1105 30-31 0.2446429210066654 1105 32-33 0.36185969801682205 1105 34-35 0.39083721087849455 1105 36-37 0.16925234809845335 1105 38-39 0.3960598566147624 1105 40-41 0.18233282542798435 1105 42-43 0.21847703203606983 1105 44-45 0.16023689144374487 1105 46-47 0.2471573475759783 1105 48-49 0.2642077037767123 1105 50-51 0.31861241568275744 1105 52-53 0.18889416063883147 1105 54-55 0.01588032864929545 1105 56-57 0.6836688950633274 1105 58-59 -6.369616653038033 1105 60-61 -8.755798218147923 1105 62-63 -2.6366016043226637 1105 64-65 -0.2854190280097022 1105 66-67 -1.3523500664985235 1105 68-69 -3.6820060692598915 1105 70-71 -4.493889309835534 1105 72-73 -6.0718320842368385 1105 74-75 -6.456747871622671 1105 76-77 -8.53338111135111 1105 78-79 -11.745947750838285 1105 80-81 -8.071802970827822 1105 82-83 -3.996612113958758 1105 84-85 -2.9814132184075 1105 86-87 -2.807965380875565 1105 88-89 -2.8109250657370453 1105 90-91 -8.110056013971466 1105 92-93 -11.065392644531464 1105 94-95 -11.3525284292866 1105 96-97 -11.79718799875214 1105 98-99 -11.34661204407293 1105 100-101 -11.02252797654021 1106 1 0.15192081621346532 1106 2 0.2593411136338908 1106 3 0.2924864410932919 1106 4 0.17973785901622108 1106 5 0.20846141973287047 1106 6 0.08620323773411087 1106 7 0.26619692659520666 1106 8 0.2561389264467451 1106 9 0.2619400639795657 1106 10-11 0.3092158612568596 1106 12-13 0.24645395374736268 1106 14-15 0.437546210815718 1106 16-17 0.3518280611802851 1106 18-19 0.2723428801727579 1106 20-21 0.18358768963533834 1106 22-23 0.2312777691180159 1106 24-25 0.3763913183067942 1106 26-27 0.3183426470801223 1106 28-29 0.3148753702350362 1106 30-31 0.3514671356521504 1106 32-33 0.4558078145705622 1106 34-35 0.3238842388079419 1106 36-37 0.28055978178624486 1106 38-39 0.1748586184668639 1106 40-41 0.40532639111258106 1106 42-43 0.23213932915245294 1106 44-45 0.3172925032015286 1106 46-47 0.18093972583691453 1106 48-49 0.3227089474361833 1106 50-51 0.3157997362233189 1106 52-53 0.24716765193499413 1106 54-55 0.10984658005163794 1106 56-57 0.8454665919375586 1106 58-59 -6.755405687855166 1106 60-61 -9.383262201805223 1106 62-63 -2.819238909128572 1106 64-65 -0.36940151187946313 1106 66-67 -1.6028467302197598 1106 68-69 -4.0148125437564275 1106 70-71 -4.615215536204669 1106 72-73 -5.7266489311556015 1106 74-75 -6.252563344204379 1106 76-77 -8.302364475294409 1106 78-79 -11.681905066382873 1106 80-81 -7.953186481983423 1106 82-83 -3.992761836691148 1106 84-85 -2.9575762044703353 1106 86-87 -2.7404665854206556 1106 88-89 -2.9919851720925976 1106 90-91 -8.328631432717565 1106 92-93 -11.481965051470358 1106 94-95 -11.842051109403872 1106 96-97 -12.272930505237127 1106 98-99 -11.522710669202496 1106 100-101 -11.21884500214057 1107 1 0.1907526276200464 1107 2 0.20504190810511247 1107 3 0.11777735338520401 1107 4 0.0347125282315659 1107 5 -0.012282321793797735 1107 6 0.027169188813736866 1107 7 0.14061726236212024 1107 8 0.13724182762746295 1107 9 0.15008552926891383 1107 10-11 0.14959982639865643 1107 12-13 0.29135070332488766 1107 14-15 0.18023821938253803 1107 16-17 0.27573297849711764 1107 18-19 0.3217559281174829 1107 20-21 0.2185608098523133 1107 22-23 0.1427865759984499 1107 24-25 0.21968037251779293 1107 26-27 0.41758180920108856 1107 28-29 0.35725643172202837 1107 30-31 0.2418023539587324 1107 32-33 0.33168284625868694 1107 34-35 0.10973969646345694 1107 36-37 0.08834306830899408 1107 38-39 0.1603631132513641 1107 40-41 0.05682667850197021 1107 42-43 0.2153065216538721 1107 44-45 0.11841169037897714 1107 46-47 0.24078986741608333 1107 48-49 0.27411719133458234 1107 50-51 0.15532242958726528 1107 52-53 0.1352817428361064 1107 54-55 0.21664392194175264 1107 56-57 0.968848614735812 1107 58-59 -6.741861812655671 1107 60-61 -8.5440733319322 1107 62-63 -2.162353521740936 1107 64-65 0.18210286314451452 1107 66-67 -1.490706778217195 1107 68-69 -4.159577736041985 1107 70-71 -4.912273084721129 1107 72-73 -6.203394413871184 1107 74-75 -6.885945370588267 1107 76-77 -9.102877867855415 1107 78-79 -12.24855018332687 1107 80-81 -8.211330466401645 1107 82-83 -4.143456609072654 1107 84-85 -3.249629269138506 1107 86-87 -2.966621243585042 1107 88-89 -2.9773168388184246 1107 90-91 -8.505150015857364 1107 92-93 -11.781342332612486 1107 94-95 -11.975253853569223 1107 96-97 -12.277147581581591 1107 98-99 -11.65999925512834 1107 100-101 -11.278891816313475 1108 1 -0.025137492391024807 1108 2 -0.047000918442190454 1108 3 -0.049880345053811226 1108 4 0.06366583544057391 1108 5 -0.08805792726285944 1108 6 -0.04650809775000653 1108 7 -0.010670416346613365 1108 8 -0.11157687033056618 1108 9 0.07999770225930547 1108 10-11 0.006888389973326525 1108 12-13 -0.09070130619118544 1108 14-15 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3.366631144364426 2312 82-83 2.1170259041869173 2312 84-85 1.7043497493709658 2312 86-87 1.5959333784058565 2312 88-89 1.646168510177766 2312 90-91 3.1478157260153843 2312 92-93 5.017544217078157 2312 94-95 9.070993545797169 2312 96-97 9.793303420472332 2312 98-99 10.224215416206249 2312 100-101 9.648765633227361 2313 1 -0.2695426602422373 2313 2 -0.3782508653413217 2313 3 -0.4058540550026386 2313 4 -0.33103127472286076 2313 5 -0.3574927191499526 2313 6 -0.2263176243201812 2313 7 -0.42174958086634007 2313 8 -0.3033404637773458 2313 9 -0.42440054873561905 2313 10-11 -0.4586207861061524 2313 12-13 -0.3550227970735804 2313 14-15 -0.4755050606019111 2313 16-17 -0.6007376516876377 2313 18-19 -0.4189741320181213 2313 20-21 -0.5201538844747162 2313 22-23 -0.5506811130745106 2313 24-25 -0.5605691317007384 2313 26-27 -0.5141099927097699 2313 28-29 -0.6652710940297837 2313 30-31 -0.6547886544046335 2313 32-33 -0.40998751982623105 2313 34-35 -0.63434762288162 2313 36-37 -0.7095033354107514 2313 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3.8215704811369697 2314 62-63 1.2803246115902027 2314 64-65 1.1932593081486047 2314 66-67 1.6832429433184544 2314 68-69 -0.30903524147426253 2314 70-71 2.3980265680346946 2314 72-73 8.230703424856852 2314 74-75 10.115839880561193 2314 76-77 8.951646524925497 2314 78-79 6.6581773175139745 2314 80-81 3.652991820919315 2314 82-83 2.1245991259550223 2314 84-85 1.5266007716528343 2314 86-87 1.5874339250744534 2314 88-89 1.6435757317691042 2314 90-91 2.623093885374626 2314 92-93 4.726244068319364 2314 94-95 8.67690810573475 2314 96-97 9.296861984358605 2314 98-99 9.546284773930562 2314 100-101 8.756630832456688 2315 1 -0.7254347927629752 2315 2 -0.5605302300635451 2315 3 -0.5361410928525636 2315 4 -0.4243415753553279 2315 5 -0.7062591361262989 2315 6 -0.8524143017339227 2315 7 -0.7744584903895699 2315 8 -0.7901690322469719 2315 9 -0.8799244279022602 2315 10-11 -0.9261061965969333 2315 12-13 -0.7141222559698335 2315 14-15 -0.754503500847882 2315 16-17 -0.9917596296576221 2315 18-19 -0.9906998182284141 2315 20-21 -0.875221613126925 2315 22-23 -0.8989849134175856 2315 24-25 -0.9092301456827983 2315 26-27 -0.8525676814245031 2315 28-29 -0.8738155181062481 2315 30-31 -0.9433891497198488 2315 32-33 -0.8353041099421148 2315 34-35 -0.9261995771477629 2315 36-37 -0.8622957524932886 2315 38-39 -0.9277982442440234 2315 40-41 -0.5079780953644288 2315 42-43 -0.5624812882721884 2315 44-45 -0.6471236984465065 2315 46-47 -0.5760457036671269 2315 48-49 -0.49552857984564014 2315 50-51 -0.4331858290356081 2315 52-53 -0.36889849495116067 2315 54-55 -0.535511062710647 2315 56-57 0.837975649928687 2315 58-59 4.087103417713603 2315 60-61 3.7968892917681494 2315 62-63 1.3049554030588197 2315 64-65 1.1274323542706561 2315 66-67 1.7698078899980132 2315 68-69 6.318808422888154 2315 70-71 5.347914165854583 2315 72-73 9.175104812650186 2315 74-75 10.091825078441186 2315 76-77 8.762819915050372 2315 78-79 6.243166958245858 2315 80-81 3.2654764211855998 2315 82-83 1.583889242480911 2315 84-85 0.9284620079930441 2315 86-87 0.9948496045232709 2315 88-89 1.1902204071160973 2315 90-91 2.638610088231985 2315 92-93 4.667564833794561 2315 94-95 8.959168369138766 2315 96-97 9.432659329132393 2315 98-99 9.376699082502448 2315 100-101 8.72731438741918 2316 1 -0.462987263224786 2316 2 -0.5219920007340448 2316 3 -0.7063995938325434 2316 4 -0.48624935203605446 2316 5 -0.7229346258033758 2316 6 -0.5525904596548514 2316 7 -0.5940493709864612 2316 8 -0.7633293212185137 2316 9 -0.9617956353775199 2316 10-11 -0.6361696996003445 2316 12-13 -0.6467550290514552 2316 14-15 -0.7191371626541425 2316 16-17 -1.0377120216758797 2316 18-19 -0.9941797590103292 2316 20-21 -0.8188007759347045 2316 22-23 -0.8663903135533886 2316 24-25 -2.1091370789819734 2316 26-27 -1.4113416835080201 2316 28-29 -1.0351160657374692 2316 30-31 -0.6894108599933446 2316 32-33 -0.685560383442585 2316 34-35 -0.7571571212750925 2316 36-37 -0.7271039001938817 2316 38-39 -1.0373137192752822 2316 40-41 -0.9489993408712039 2316 42-43 -0.9697869676080941 2316 44-45 -0.8246073380913259 2316 46-47 -0.8327359355177038 2316 48-49 -1.1603392497558431 2316 50-51 -1.324553127369363 2316 52-53 -1.172749228885813 2316 54-55 -1.495021379964932 2316 56-57 -0.16949575841648112 2316 58-59 3.554881056313274 2316 60-61 3.520815879031499 2316 62-63 0.897420882794421 2316 64-65 0.510201893460124 2316 66-67 5.650843339229052 2316 68-69 8.603462449411595 2316 70-71 5.11290289242779 2316 72-73 8.912618032996082 2316 74-75 9.900972027474413 2316 76-77 8.552850215573066 2316 78-79 5.903498013844427 2316 80-81 2.8570755644278343 2316 82-83 1.2976873160795535 2316 84-85 0.9592831260857331 2316 86-87 0.8935049550650618 2316 88-89 0.858964023658622 2316 90-91 2.072854666614891 2316 92-93 4.079697710598506 2316 94-95 8.053886975564112 2316 96-97 8.9622675194555 2316 98-99 9.259560158063316 2316 100-101 8.135328694298975 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 11.0 4 36.0 5 59.0 6 165.0 7 424.0 8 1011.0 9 1816.0 10 2916.0 11 4449.0 12 6271.0 13 8303.0 14 11174.0 15 15157.0 16 19909.0 17 26107.0 18 29209.0 19 31236.0 20 32731.0 21 32988.0 22 31640.0 23 30624.0 24 31328.0 25 33923.0 26 44708.0 27 64422.0 28 89163.0 29 116403.0 30 135968.0 31 147111.0 32 152183.0 33 156555.0 34 158192.0 35 150675.0 36 123982.0 37 75273.0 38 23684.0 39 1141.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.90514327585321 19.714140825184916 14.315186771305957 24.065529127655914 2 15.886078741335824 20.846960467397903 39.141670635915546 24.125290155350722 3 19.348617285853244 24.146545476068432 30.272299148248532 26.232538089829795 4 13.194267629729886 17.029490460939208 36.969500020101044 32.80674188922986 5 13.276761979043867 37.31915920694321 35.142979740020586 14.261099073992337 6 32.42859934671543 36.70772495044529 17.02297663251347 13.840699070325805 7 28.265246639775942 32.401929253268655 20.90966145379664 18.423162653158766 8 26.024725521694435 35.80175360131506 19.66416574955205 18.50935512743845 9 25.75512911568819 16.429809397460122 20.085462926979616 37.729598559872066 10-11 24.845473245514118 26.533206920118463 27.811102698453112 20.810217135914307 12-13 25.60724687205464 23.686955317618786 28.064571244790482 22.641226565536098 14-15 22.147773734294276 25.482516935867743 26.01807143983841 26.35163788999957 16-17 22.072450852954184 28.58602575613417 26.85683368398483 22.48468970692682 18-19 22.015221747203746 27.207065401137793 28.882959611083546 21.89475324057491 20-21 22.548510512844565 26.51676315166459 28.94106039692856 21.993665938562284 22-23 22.212348516319814 26.505316725406374 28.891421992325867 22.39091276594794 24-25 22.184737502735974 26.66319365343125 28.94700695496027 22.20506188887251 26-27 21.99344537632133 26.924963545873293 28.69550481462487 22.38608626318051 28-29 22.006388374175245 27.318549597285624 28.62718635419601 22.04787567434312 30-31 22.184036287712917 26.92326162791022 28.48929444343 22.403407640946863 32-33 21.895477114767463 26.916171467896184 28.565717308854673 22.622634108481684 34-35 22.455356382579254 27.029116379809746 28.436203800978653 22.079323436632347 36-37 22.536157174803243 26.64592218109193 28.50496728317424 22.312953360930592 38-39 22.069645144967925 26.87413363834476 28.626124943731867 22.430096272955442 40-41 22.36501013266998 26.760944107371394 28.65338114505175 22.22066461490688 42-43 22.163138342567787 26.797884262182375 28.42146533916899 22.617512056080848 44-45 22.236200480430096 26.858399344532945 28.60234607610589 22.303054098931067 46-47 22.526587641686028 27.08215520541943 28.00466252412457 22.386594628769974 48-49 22.221784716433415 27.20242508527872 27.942321843221197 22.633468355066665 50-51 22.19484365828499 27.284361695651516 28.088591497615518 22.432203148447975 52-53 22.494435491775196 27.04969508645058 27.995082066548477 22.46078735522575 54-55 21.98488505471112 27.160969197142087 28.130183092660992 22.7239626554858 56-57 22.18594623430734 27.125088037693974 28.10283417766573 22.586131550332954 58-59 20.74619408158305 26.74209107195909 29.70129954896153 22.81041529749633 60-61 21.87687546821068 26.93516113448761 28.453486988118826 22.73447640918288 62-63 21.826217201278098 27.079484684740017 28.246300481450408 22.847997632531474 64-65 21.159096608998514 27.07810434061499 28.639106327690474 23.123692722696028 66-67 21.5012621742027 26.674165310830222 29.02182065796212 22.802751857004957 68-69 22.414479586051485 25.703877537838828 29.575473119900103 22.306169756209588 70-71 21.493398703894997 26.082157456943538 29.582891505310215 22.84155233385125 72-73 21.259897186207233 26.243125284356932 29.563731377555158 22.933246151880677 74-75 21.77936411448061 26.493603673700132 28.77834833832239 22.94868387349687 76-77 20.23269545347884 26.837687254667475 29.46074213714647 23.46887515470721 78-79 21.25342333811603 27.02748400385943 28.2653395129838 23.45375314504074 80-81 21.939497964206666 27.045955549798208 27.43418569383366 23.58036079216147 82-83 22.01493006088108 27.019479321176103 27.364938338608464 23.60065227933435 84-85 21.978618075749658 27.044108384814336 27.245649241297365 23.73162429813864 86-87 22.081011291259667 27.01409376354941 27.09720846826328 23.807686476927643 88-89 21.957518112578704 27.047912799425333 26.885205992457085 24.10936309553888 90-91 21.715579499256766 26.977715269444992 27.22338010638107 24.08332512491717 92-93 21.613748948395294 26.724435937136416 27.52882963108812 24.13298548338017 94-95 20.3289747819192 26.463185879209956 28.85022897882713 24.35761036004371 96-97 20.11419921821418 26.24364668213344 29.087568666458697 24.554585433193683 98-99 20.45120123741034 26.45356650981859 28.34242803984466 24.752804212926414 100-101 20.33474888927265 26.414761628154725 28.327472251440412 24.923017231132214 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 122.0 1 96.5 2 72.5 3 88.0 4 111.0 5 131.0 6 167.5 7 256.5 8 384.5 9 520.5 10 609.5 11 655.5 12 762.0 13 891.0 14 1028.0 15 1165.0 16 1337.0 17 1538.0 18 1800.0 19 2114.5 20 2462.5 21 2999.5 22 3820.5 23 4774.5 24 6039.5 25 7721.5 26 9851.0 27 12321.0 28 14953.0 29 18185.0 30 21694.0 31 25645.5 32 30136.0 33 33886.5 34 36725.0 35 39972.5 36 43715.0 37 47005.5 38 49989.5 39 52882.5 40 55799.0 41 58666.0 42 61174.0 43 63528.5 44 65878.0 45 68011.0 46 69576.0 47 70781.0 48 71318.0 49 70576.5 50 69245.0 51 67580.0 52 65720.0 53 63792.5 54 60951.0 55 56723.5 56 51345.0 57 45175.0 58 38950.5 59 32621.0 60 26446.5 61 21088.0 62 16214.0 63 11985.5 64 8559.5 65 6041.5 66 4339.0 67 3086.0 68 2233.0 69 1555.0 70 1020.0 71 721.5 72 521.0 73 389.5 74 274.0 75 189.0 76 122.0 77 68.5 78 45.0 79 30.0 80 16.0 81 10.0 82 7.5 83 4.5 84 2.0 85 1.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023451214772925236 2 0.005639458790632022 3 0.0030151561850903876 4 0.0 5 0.062424900276500994 6 1.1167245129964399E-4 7 0.0 8 5.025260308483979E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.5126301542419897E-4 16-17 0.0 18-19 0.001535496205370105 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.39590564124555E-4 28-29 0.0023730395901174345 30-31 0.008542942524422765 32-33 2.5126301542419897E-4 34-35 0.001032970174521707 36-37 0.02434459438332239 38-39 0.023339542321625596 40-41 0.025489237009143743 42-43 0.025991763039992136 44-45 0.026410534732365806 46-47 0.02822521206598502 48-49 0.027890194712086088 50-51 0.023395378547275414 52-53 0.020240631798060474 54-55 0.015299125828051227 56-57 0.1482730972131023 58-59 0.7660730159155578 60-61 0.31070067762843445 62-63 5.30444143673309E-4 64-65 0.1477147349566041 66-67 1.4374477931290173 68-69 2.746556021601919 70-71 2.2169773394261822 72-73 1.8815412138348766 74-75 1.5779596549767947 76-77 1.1317165395834172 78-79 0.43482460724798877 80-81 2.2334490259928798E-4 82-83 8.3754338474733E-5 84-85 0.0 86-87 0.0029872380722654767 88-89 2.7918112824910997E-5 90-91 0.2327253885084581 92-93 0.17912261188462894 94-95 1.3082706850881543 96-97 1.2885046612081172 98-99 1.0171964407756322 100-101 0.41519817393207636 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1790952.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.81419321601942 #Duplication Level Percentage of deduplicated Percentage of total 1 78.7606441014526 49.472863164067846 2 12.621295804679425 15.855930266233372 3 4.0283085498749305 7.591048547567607 4 1.6880903121788495 4.241441241411711 5 0.8840899525370659 2.776669854950235 6 0.5017964413176109 1.891196317202122 7 0.313240693701173 1.3773173020085807 8 0.23411586252880984 1.1764639219055761 9 0.16692368251888018 0.943665880146338 >10 0.7373910143852027 8.062352070398807 >50 0.0390298439037755 1.6533807885361795 >100 0.02125119809979996 2.4830305615679458 >500 0.0025779939961510213 1.1206992008924976 >1k 0.0012445488257280788 1.3539408831111301 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3987 0.2226190316658403 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3383 0.1888939513733478 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2342 0.1307684404718831 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 1796 0.10028186126708029 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.67508676949466E-4 2 0.0 0.0 0.0 0.0 1.67508676949466E-4 3 0.0 0.0 0.0 0.0 1.67508676949466E-4 4 0.0 0.0 0.0 5.5836225649821994E-5 1.67508676949466E-4 5 0.0 0.0 0.0 5.5836225649821994E-5 1.67508676949466E-4 6 0.0 0.0 0.0 5.5836225649821994E-5 1.67508676949466E-4 7 0.0 0.0 0.0 5.5836225649821994E-5 1.67508676949466E-4 8 0.0 0.0 0.0 5.5836225649821994E-5 1.67508676949466E-4 9 0.0 0.0 0.0 5.5836225649821994E-5 1.67508676949466E-4 10-11 0.0 0.0 0.0 5.5836225649821994E-5 2.2334490259928798E-4 12-13 0.0 0.0 0.0 5.5836225649821994E-5 4.46689805198576E-4 14-15 0.0 0.0 0.0 5.5836225649821994E-5 5.5836225649822E-4 16-17 0.0 0.0 0.0 5.5836225649821994E-5 5.5836225649822E-4 18-19 0.0 0.0 0.0 1.39590564124555E-4 6.141984821480419E-4 20-21 0.0 0.0 0.0 2.23344902599288E-4 6.97952820622775E-4 22-23 0.0 0.0 0.0 4.18771692373665E-4 7.537890462725969E-4 24-25 0.0 0.0 0.0 7.537890462725969E-4 8.375433847473299E-4 26-27 0.0 0.0 0.0 0.001256315077120995 8.375433847473299E-4 28-29 0.0 0.0 0.0 0.007509972349901058 8.375433847473299E-4 30-31 0.0 0.0 0.0 0.026382616619540895 8.375433847473299E-4 32-33 0.0 0.0 0.0 0.06892982056470526 8.933796103971519E-4 34-35 0.0 0.0 0.0 0.13744086943703682 0.0010050520616967959 36-37 0.0 0.0 0.0 0.25042547203945165 0.0010050520616967959 38-39 0.0 0.0 0.0 0.42823593262130977 0.0011446426258213509 40-41 0.0 0.0 0.0 0.6241094122008853 0.0012283969642960838 42-43 0.0 0.0 0.0 0.8148459590206772 0.0013121513027708168 44-45 0.0 0.0 0.0 1.0481576278984586 0.0014238237540704609 46-47 0.0 0.0 0.0 1.3103924616628473 0.0015075780925451938 48-49 0.0 0.0 0.0 1.56684824607248 0.0016750867694946597 50-51 0.0 0.0 0.0 1.8316236281039358 0.0017309229951444818 52-53 0.0 0.0 0.0 2.082830807302485 0.0018705135592690368 54-55 0.0 0.0 0.0 2.342162157333083 0.0018984316720939479 56-57 0.0 0.0 0.0 2.570336893451081 0.0018984316720939479 58-59 0.0 0.0 0.0 2.7263433079166832 0.0018984316720939479 60-61 0.0 5.5836225649821994E-5 0.0 2.8442135802634576 0.0018984316720939479 62-63 0.0 5.5836225649821994E-5 0.0 2.9455563298178844 0.0018984316720939479 64-65 0.0 5.5836225649821994E-5 0.0 3.0460056997619143 0.00195426789774377 66-67 0.0 5.5836225649821994E-5 0.0 3.1347573804323066 0.00195426789774377 68-69 0.0 5.5836225649821994E-5 0.0 3.2391432042846486 0.00195426789774377 70-71 0.0 5.5836225649821994E-5 5.5836225649821994E-5 3.4243519647651084 0.00195426789774377 72-73 0.0 5.5836225649821994E-5 5.5836225649821994E-5 3.661628005664027 0.0020659403490434136 74-75 0.0 5.5836225649821994E-5 5.5836225649821994E-5 3.9236953307514666 0.0021496946875181468 76-77 0.0 5.5836225649821994E-5 5.5836225649821994E-5 4.299054357682394 0.002205530913167969 78-79 0.0 5.5836225649821994E-5 5.5836225649821994E-5 4.834579597889837 0.002261367138817791 80-81 0.0 5.5836225649821994E-5 5.5836225649821994E-5 5.417621466125279 0.0023451214772925236 82-83 0.0 5.5836225649821994E-5 5.5836225649821994E-5 5.976123313187623 0.0024567939285921677 84-85 0.0 5.5836225649821994E-5 5.5836225649821994E-5 6.424906976848067 0.0024567939285921677 86-87 0.0 5.5836225649821994E-5 5.5836225649821994E-5 6.826564866060062 0.00251263015424199 88-89 0.0 5.5836225649821994E-5 5.5836225649821994E-5 7.234448494432011 0.0025684663798918117 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3040 0.0 37.70111 1 GTATCAA 5010 0.0 36.150555 1 CAACGCA 6115 0.0 28.910128 5 TCAACGC 6130 0.0 28.839384 4 ATCAACG 6140 0.0 28.794827 3 AACGCAG 6380 0.0 27.842321 6 TATCAAC 6365 0.0 27.778494 2 CGCAGAG 7305 0.0 24.123268 8 ACGCAGA 7340 0.0 24.072432 7 TACATGG 4910 0.0 23.334616 2 GTACATG 5255 0.0 22.079226 1 ACATGGG 5045 0.0 21.774406 3 TAAGGTG 1100 0.0 21.428535 5 AGAGTAC 6810 0.0 20.860512 10-11 GCAGAGT 8385 0.0 20.791386 9 GGTAAGG 1010 0.0 20.539171 3 GCGTAAT 65 0.0065367664 18.738213 68-69 CGCGCGA 65 0.0067862133 18.617025 72-73 GAGTACT 4750 0.0 18.59964 12-13 AGTACTT 5095 0.0 18.403727 12-13 >>END_MODULE