FastQCFastQC Report
Fri 27 May 2016
SRR939804_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939804_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1909270
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36820.19284857563361912No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29730.15571396397576037No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33450.037.9695631
GTATCAA53500.035.5218471
TCAACGC66050.028.4890444
ATCAACG66750.028.260763
TATCAAC67000.028.1553062
CAACGCA67600.027.696645
AACGCAG70300.026.833656
ACGCAGA79300.023.6102497
TACATGG54300.023.5645852
GTACATG56200.023.352631
CGCAGAG80150.023.2412578
ACATGGG57550.022.1520883
GCAGAGT86300.020.4403539
GTAGCCC9850.020.0587463
GAGTACT48750.019.49249512-13
AGAGTAC71200.019.39106810-11
AGTACTT51300.018.5235712-13
GAGTACA40350.018.3030741
CAGAGTA82000.018.12787410-11
GTACTTT52200.018.07186314-15
Produced by FastQC (version 0.11.5)