FastQCFastQC Report
Fri 27 May 2016
SRR939804_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939804_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1909270
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41650.21814620247529162No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36740.19242956732154173No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24680.12926406427587508No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33900.039.615121
GTATCAA57300.036.8535771
ATCAACG68400.029.8213523
TCAACGC69650.029.2861524
CAACGCA70350.028.9947475
TATCAAC71450.028.9527762
AACGCAG73700.027.8055326
ACGCAGA82900.024.3192047
CGCAGAG83200.024.2315168
TACATGG54100.023.6794972
GTACATG57200.023.1463431
GTACGGG1503.0572766E-522.1452711
ACATGGG55350.022.0257383
GCAGAGT93700.020.959259
GAGTACT51400.020.76566112-13
AGAGTAC76850.020.52457410-11
GTAGGAC4400.019.4059473
GTACTTT54400.019.31528714-15
CTATACT7000.018.9747034
CCGATCG1507.7931286E-418.97479
Produced by FastQC (version 0.11.5)