##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939804_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1909270 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.345928024847193 31.0 31.0 34.0 30.0 34.0 2 31.5721809906404 31.0 31.0 34.0 30.0 34.0 3 31.619481791470037 31.0 31.0 34.0 30.0 34.0 4 35.444000062851245 37.0 35.0 37.0 33.0 37.0 5 35.16409517773809 37.0 35.0 37.0 32.0 37.0 6 35.184755953846235 37.0 35.0 37.0 32.0 37.0 7 35.08791684780047 37.0 35.0 37.0 32.0 37.0 8 35.04283155342094 37.0 35.0 37.0 32.0 37.0 9 36.6342555007935 39.0 35.0 39.0 32.0 39.0 10-11 36.48624290959372 38.0 35.0 39.0 32.0 39.0 12-13 36.41222954322856 38.0 35.0 39.0 32.0 39.0 14-15 37.44822183347563 39.0 36.0 41.0 32.0 41.0 16-17 37.3657924232822 39.0 36.0 41.0 32.0 41.0 18-19 37.32409245418406 39.0 36.0 41.0 32.0 41.0 20-21 37.22785881514925 39.0 36.0 40.0 31.0 41.0 22-23 37.10132720882851 39.0 36.0 40.0 31.0 41.0 24-25 36.954169394585364 39.0 36.0 40.0 31.0 41.0 26-27 36.655148302754455 38.5 35.5 40.0 30.0 41.0 28-29 36.43126247204429 38.0 35.0 40.0 30.0 41.0 30-31 36.16234346111341 38.0 35.0 40.0 29.5 41.0 32-33 36.14593195305012 38.0 35.0 40.0 30.0 41.0 34-35 36.01698266876869 38.0 35.0 40.0 29.5 41.0 36-37 36.069822497603795 38.0 35.0 40.0 30.0 41.0 38-39 35.963378935404634 38.0 35.0 40.0 29.0 41.0 40-41 35.89233345728996 38.0 35.0 40.0 29.0 41.0 42-43 35.68041502773311 38.0 34.0 40.0 28.0 41.0 44-45 35.45550576922069 38.0 34.0 40.0 27.0 41.0 46-47 35.211579556584454 38.0 34.0 40.0 27.0 41.0 48-49 34.925362573130045 38.0 33.0 40.0 26.0 41.0 50-51 34.71042021296097 38.0 33.0 40.0 26.0 41.0 52-53 34.45770503909871 38.0 33.0 40.0 24.5 41.0 54-55 34.194694045368124 37.0 33.0 40.0 24.0 41.0 56-57 33.8223446657623 37.0 32.5 40.0 23.5 41.0 58-59 33.44836979578582 36.5 32.0 39.0 23.0 41.0 60-61 33.12814950216575 36.0 31.0 39.0 22.0 40.5 62-63 32.831533518046164 36.0 31.0 39.0 22.0 40.0 64-65 32.39126472421397 35.0 31.0 39.0 20.5 40.0 66-67 31.45927972471154 35.0 29.5 38.0 15.0 40.0 68-69 29.509402546523017 34.0 26.5 37.0 7.5 39.5 70-71 28.841796079129722 33.0 25.5 36.5 8.5 39.0 72-73 29.418510739706797 33.5 27.0 36.0 9.0 39.0 74-75 29.538100687697394 34.0 28.0 36.0 8.0 39.0 76-77 29.103199914103296 32.5 27.5 35.0 8.0 37.5 78-79 29.641309243847125 34.0 29.0 35.0 8.0 37.5 80-81 29.45266620226579 34.0 29.0 35.0 7.0 37.0 82-83 28.998970548953267 33.5 28.5 35.0 4.5 36.5 84-85 28.620812666621273 33.0 28.0 35.0 2.0 36.0 86-87 28.222953537215794 33.0 27.0 35.0 2.0 36.0 88-89 27.75084980123293 33.0 26.5 35.0 2.0 35.0 90-91 27.244007133616513 32.5 26.0 35.0 2.0 35.0 92-93 26.735287570642186 32.0 25.0 34.0 2.0 35.0 94-95 25.889591571647802 32.0 23.5 34.0 2.0 35.0 96-97 25.339162611888312 32.0 21.5 34.0 2.0 35.0 98-99 24.64200558328576 31.0 18.0 34.0 2.0 35.0 100-101 23.14471499578373 30.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.24425922788103094 1101 2 0.3283410336835608 1101 3 0.32792683165667924 1101 4 0.12394994489034872 1101 5 0.20221114249261518 1101 6 0.18102405380864184 1101 7 0.23290408935798013 1101 8 0.2241455164514221 1101 9 0.4842653961509953 1101 10-11 0.4679044919437203 1101 12-13 0.4526850042155175 1101 14-15 0.4654248178119005 1101 16-17 0.5008363550846155 1101 18-19 0.2685950425783403 1101 20-21 0.31119934273928607 1101 22-23 0.37993798055984485 1101 24-25 0.4054136289344612 1101 26-27 0.4441452754912021 1101 28-29 0.3301144660860089 1101 30-31 0.23801655102565888 1101 32-33 0.16782157239089912 1101 34-35 0.14911718034463206 1101 36-37 0.3989937918218871 1101 38-39 0.4097925939514866 1101 40-41 0.5162937875920406 1101 42-43 0.1095136965680581 1101 44-45 0.07031248942105606 1101 46-47 0.13755259089956695 1101 48-49 0.3091083047802954 1101 50-51 0.06793592199040432 1101 52-53 -0.01617185533040555 1101 54-55 -0.06191610327174857 1101 56-57 -0.1442633347708906 1101 58-59 -0.16978514071594475 1101 60-61 -0.2511302792319725 1101 62-63 -0.1811155581160122 1101 64-65 -0.31883896255795463 1101 66-67 -0.44744307874873 1101 68-69 -8.876964855253814 1101 70-71 -6.5272378282714385 1101 72-73 -1.8051545715191963 1101 74-75 -0.6630187409349197 1101 76-77 -0.6297302344844518 1101 78-79 -0.6333321199250577 1101 80-81 -0.5424550273260884 1101 82-83 -0.4565577410140875 1101 84-85 -0.41855913782493914 1101 86-87 -0.47982554803196464 1101 88-89 -0.4104717928436763 1101 90-91 -0.7245682998174843 1101 92-93 -0.8159219779904952 1101 94-95 -0.48225079187453446 1101 96-97 -0.7918257521293448 1101 98-99 -0.8008184800327882 1101 100-101 -0.6487693901378826 1102 1 0.2100915978537401 1102 2 0.11887133071535416 1102 3 0.2749858104416312 1102 4 0.2648067287966853 1102 5 0.22313876639663022 1102 6 0.305065048102378 1102 7 0.29990436912846263 1102 8 0.2485895386007968 1102 9 0.4206009834842561 1102 10-11 0.28174521280140397 1102 12-13 0.30840987369995077 1102 14-15 0.4498055748813812 1102 16-17 0.440313817724693 1102 18-19 0.3552110602302747 1102 20-21 0.2781251930040085 1102 22-23 0.15284220931707893 1102 24-25 0.25402876504544025 1102 26-27 0.29230604850232567 1102 28-29 0.302591385021195 1102 30-31 0.2996242547434349 1102 32-33 0.23193876455098916 1102 34-35 0.0478904131717357 1102 36-37 2.168441113070685E-5 1102 38-39 0.28132489160616814 1102 40-41 0.14945793896371384 1102 42-43 0.13793177924287647 1102 44-45 0.19919905568706753 1102 46-47 0.0721935873047741 1102 48-49 0.03977471279122824 1102 50-51 0.14532690387922287 1102 52-53 0.05425878006531093 1102 54-55 -0.11058019921718909 1102 56-57 -0.14583361258825533 1102 58-59 -0.1763289986981178 1102 60-61 -0.07513894155956535 1102 62-63 -0.0390978586739692 1102 64-65 -0.044098805992057066 1102 66-67 0.19362247728190596 1102 68-69 -4.786670647558541 1102 70-71 0.14274161123742957 1102 72-73 0.6620590992278963 1102 74-75 0.40431607570983985 1102 76-77 0.1774248956088229 1102 78-79 0.024008292129355624 1102 80-81 9.171835469565792E-4 1102 82-83 0.04177538117171409 1102 84-85 0.06924999536164833 1102 86-87 0.1413648225847446 1102 88-89 0.18181538363871041 1102 90-91 -0.03724863300829284 1102 92-93 -0.1446786885230651 1102 94-95 0.09443231790634599 1102 96-97 -0.34469379547793366 1102 98-99 -0.3789234045211529 1102 100-101 -0.46445501257442245 1103 1 0.11744646359517574 1103 2 0.08259048176464745 1103 3 0.13935233037479833 1103 4 0.15839795789233335 1103 5 0.24528561024700934 1103 6 0.2305596917261994 1103 7 -0.0070596958205086935 1103 8 0.24194411466858412 1103 9 0.3003277074304407 1103 10-11 0.3000655194416524 1103 12-13 0.19444462936539963 1103 14-15 0.3284474451386217 1103 16-17 0.275993593848348 1103 18-19 0.228863560475979 1103 20-21 0.1691851399316633 1103 22-23 0.15000366616863658 1103 24-25 0.1917799664151758 1103 26-27 0.15394588587125924 1103 28-29 0.11909864496708877 1103 30-31 0.2041766572242807 1103 32-33 0.1721060252210691 1103 34-35 -0.14392009887816926 1103 36-37 -0.05130396050069663 1103 38-39 0.06850553933714565 1103 40-41 0.03313974280292342 1103 42-43 -0.09177709873726769 1103 44-45 -0.008524885322962916 1103 46-47 -0.03702540597583237 1103 48-49 -0.10567857474607933 1103 50-51 -0.16219801581393511 1103 52-53 -0.09685491167019933 1103 54-55 -0.14237718454212 1103 56-57 -0.014063266893792559 1103 58-59 -0.0642502540195764 1103 60-61 -0.1623745496931832 1103 62-63 -0.3834033143668307 1103 64-65 -0.35246959861158444 1103 66-67 -0.08842448088611476 1103 68-69 -4.554908060979741 1103 70-71 0.37737429470172046 1103 72-73 0.4842127969637886 1103 74-75 -0.047235876742295346 1103 76-77 -0.1792010012156311 1103 78-79 -0.19866138679843814 1103 80-81 -0.15902392476512617 1103 82-83 -0.3676562118781881 1103 84-85 -0.3047758476662743 1103 86-87 -0.30573055246503955 1103 88-89 -0.325635824455599 1103 90-91 -0.4629991981305501 1103 92-93 -0.6122274804287535 1103 94-95 -0.3717142724795899 1103 96-97 -1.0012294279234517 1103 98-99 -0.516961822489197 1103 100-101 -0.5482751873097058 1104 1 0.0959713501050743 1104 2 0.1670213653512711 1104 3 0.12676752267829627 1104 4 0.1814289120817989 1104 5 0.20017515448187595 1104 6 0.1887617189796842 1104 7 0.07987054817576933 1104 8 0.045446838629324304 1104 9 0.24160562877173675 1104 10-11 0.20730397161319303 1104 12-13 0.27647097552954136 1104 14-15 0.2268414536149308 1104 16-17 0.2396959741160387 1104 18-19 0.15636525117604094 1104 20-21 0.24803931630787446 1104 22-23 0.18433033123550047 1104 24-25 0.10054327138177399 1104 26-27 0.18545475882382334 1104 28-29 0.14485789820322026 1104 30-31 0.08613356569245667 1104 32-33 0.27245056113630284 1104 34-35 0.4608371146101433 1104 36-37 0.4107343693057999 1104 38-39 0.44558011755457727 1104 40-41 0.21088066012422502 1104 42-43 0.2862274884641778 1104 44-45 0.505728435092955 1104 46-47 0.5103501416424123 1104 48-49 0.4809883960152206 1104 50-51 0.27068243136384496 1104 52-53 0.29951620641305254 1104 54-55 0.30915004812399616 1104 56-57 0.35392342665219445 1104 58-59 0.2758423675868755 1104 60-61 0.21618323357592573 1104 62-63 0.4123772238410055 1104 64-65 0.47700669093145365 1104 66-67 0.5786526309023827 1104 68-69 -3.762155041022538 1104 70-71 0.9345868905659032 1104 72-73 1.2740864208529317 1104 74-75 0.8491925069881141 1104 76-77 0.7210014613559395 1104 78-79 0.38416680237751066 1104 80-81 0.24554708860379648 1104 82-83 0.12498663988162306 1104 84-85 0.09856878840186667 1104 86-87 0.18778764561559314 1104 88-89 0.20734001588678552 1104 90-91 0.15811219173397362 1104 92-93 -0.1180294077276578 1104 94-95 0.2950435399429381 1104 96-97 -0.14527496576439347 1104 98-99 -0.055239569306614555 1104 100-101 -0.03309830339386721 1105 1 0.11909119295761172 1105 2 0.17702220124203194 1105 3 0.1279223455689653 1105 4 0.13380154820263357 1105 5 0.13842670983575545 1105 6 0.15847759572958608 1105 7 0.022378180654506252 1105 8 0.2761671177372307 1105 9 0.26181577568948455 1105 10-11 0.18902607574925412 1105 12-13 0.1441790725247145 1105 14-15 0.2765040622309698 1105 16-17 0.24421898897499972 1105 18-19 0.24436166082620048 1105 20-21 0.3014544773760619 1105 22-23 0.26862692916010644 1105 24-25 0.19169292966167006 1105 26-27 0.1378181901952047 1105 28-29 0.373224995552647 1105 30-31 0.3592449501265804 1105 32-33 0.18651850313343488 1105 34-35 0.12878398778163103 1105 36-37 -0.13762041855706997 1105 38-39 0.19498131128619178 1105 40-41 -0.04944209322034254 1105 42-43 0.09455027365219593 1105 44-45 0.059183898037126426 1105 46-47 0.0997023860091133 1105 48-49 0.09645575846143828 1105 50-51 0.19886896635781426 1105 52-53 0.08904662487589121 1105 54-55 -8.743718275212586E-4 1105 56-57 -0.1991270273161021 1105 58-59 -0.08841021670872351 1105 60-61 -0.06645158366253412 1105 62-63 -0.13571983103721408 1105 64-65 -0.08306798197652654 1105 66-67 0.561038720884028 1105 68-69 1.8069519788676836 1105 70-71 1.8938023871845253 1105 72-73 0.9921566376670725 1105 74-75 0.5687275228501356 1105 76-77 0.25927854123127503 1105 78-79 -0.023987212485419462 1105 80-81 0.06854547851897408 1105 82-83 0.0911885413340876 1105 84-85 -0.19447286881127468 1105 86-87 -0.09015971515884047 1105 88-89 0.09469030188084204 1105 90-91 0.08648580704112518 1105 92-93 0.0885440770213144 1105 94-95 0.192791709939236 1105 96-97 -0.09349078678824085 1105 98-99 0.353328908058657 1105 100-101 0.36851287604799765 1106 1 0.370855110237283 1106 2 0.35323370373752283 1106 3 0.3528982759829624 1106 4 0.28588734185078835 1106 5 0.4083127725573803 1106 6 0.45155741189959997 1106 7 0.37673465715167964 1106 8 0.338773336148364 1106 9 0.46847115455030774 1106 10-11 0.38177326525950406 1106 12-13 0.363926092875964 1106 14-15 0.5561080720896214 1106 16-17 0.5030954886801524 1106 18-19 0.5696646846618094 1106 20-21 0.4036043775406384 1106 22-23 0.3611897037470442 1106 24-25 0.5082056142149227 1106 26-27 0.5061603213772159 1106 28-29 0.6548304567006156 1106 30-31 0.762200252628709 1106 32-33 0.7800974173856403 1106 34-35 0.6252288822627818 1106 36-37 0.8042086486496416 1106 38-39 0.7917493468759815 1106 40-41 0.7953347670938555 1106 42-43 0.7205713537086638 1106 44-45 0.8116149657465783 1106 46-47 0.7757423581215903 1106 48-49 0.8135843714683375 1106 50-51 0.8118604562375182 1106 52-53 0.8504379813255127 1106 54-55 1.0495579321654134 1106 56-57 0.9241886945106259 1106 58-59 0.9896483597313761 1106 60-61 0.9107170899886654 1106 62-63 0.9020306863205505 1106 64-65 0.9946629477181474 1106 66-67 1.46512873244059 1106 68-69 2.9120973370341865 1106 70-71 3.2413458665795325 1106 72-73 2.1069361539355853 1106 74-75 1.7116316982316526 1106 76-77 1.0740897550498225 1106 78-79 1.1639788640218711 1106 80-81 1.1941294327606755 1106 82-83 1.4277228529439263 1106 84-85 1.3813317035405568 1106 86-87 1.2927700079737328 1106 88-89 1.3360185319876656 1106 90-91 1.35689555123162 1106 92-93 1.309320674901926 1106 94-95 1.4730138615468 1106 96-97 1.3065042332974137 1106 98-99 1.3796955783614493 1106 100-101 1.4594073793292104 1107 1 0.04242878926056903 1107 2 0.09128399700253809 1107 3 0.11124116009271745 1107 4 0.12411564874692971 1107 5 0.0615866824044744 1107 6 0.0030738183678025166 1107 7 0.0013506196291643846 1107 8 0.07036762558665544 1107 9 0.034702979984587046 1107 10-11 0.17017942526951657 1107 12-13 0.15510679356587076 1107 14-15 0.15473990330252008 1107 16-17 0.1140478214773637 1107 18-19 0.13069173823925695 1107 20-21 0.16647665717452753 1107 22-23 0.08961618524101311 1107 24-25 0.13931169043492986 1107 26-27 0.241325718352428 1107 28-29 0.08895056533869194 1107 30-31 0.1246943536197449 1107 32-33 0.24123819743527264 1107 34-35 0.0016277901034129627 1107 36-37 -0.21137597122945095 1107 38-39 9.98431125061927E-4 1107 40-41 -0.18091068625501805 1107 42-43 -0.1309453737940416 1107 44-45 0.007424341880224006 1107 46-47 0.14344725036635708 1107 48-49 0.08616593830933539 1107 50-51 0.131727194815916 1107 52-53 0.14937768462542778 1107 54-55 0.16367084963066247 1107 56-57 0.17529144802355034 1107 58-59 0.02000899383872934 1107 60-61 0.20847057080524678 1107 62-63 0.27908410325127164 1107 64-65 0.29485508686734363 1107 66-67 0.6752955740710576 1107 68-69 1.9408339378566772 1107 70-71 2.1675560863918584 1107 72-73 1.4592514547609667 1107 74-75 0.8374575594869604 1107 76-77 0.3097599787787644 1107 78-79 0.4669030067794928 1107 80-81 0.5497297191234516 1107 82-83 0.535486510554211 1107 84-85 0.4661329671323564 1107 86-87 0.47155372060932876 1107 88-89 0.4075590641986615 1107 90-91 0.30177272343131634 1107 92-93 0.3145980808147364 1107 94-95 0.7065451929089832 1107 96-97 0.2308733202431874 1107 98-99 0.6288681119336346 1107 100-101 0.6863450633221042 1108 1 -0.10800126466989823 1108 2 -0.17987464592678037 1108 3 -0.1798825184227688 1108 4 -0.05450407197663765 1108 5 -0.026922868021713953 1108 6 -0.027165801767765174 1108 7 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64-65 -0.37959178690892514 2316 66-67 0.1840096235859221 2316 68-69 1.5774936428560231 2316 70-71 1.767135666951404 2316 72-73 0.9585852014432987 2316 74-75 0.40267097810071917 2316 76-77 0.016824983347362377 2316 78-79 -0.1277245440189212 2316 80-81 -0.19970246988581764 2316 82-83 -0.0852837326690441 2316 84-85 -0.23893436524375034 2316 86-87 -0.23532962795264822 2316 88-89 -0.25346647641831765 2316 90-91 -0.28683461677666244 2316 92-93 -0.27801384974263144 2316 94-95 0.05501852102106497 2316 96-97 0.0054871652590691156 2316 98-99 -0.19383073068917156 2316 100-101 -0.33127339379837295 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 16.0 4 24.0 5 66.0 6 171.0 7 482.0 8 1090.0 9 1911.0 10 3086.0 11 4718.0 12 6548.0 13 8246.0 14 9968.0 15 12041.0 16 14485.0 17 16747.0 18 16517.0 19 14917.0 20 14881.0 21 15925.0 22 17245.0 23 19825.0 24 23633.0 25 28952.0 26 35956.0 27 44480.0 28 54091.0 29 65634.0 30 77984.0 31 92549.0 32 109946.0 33 133508.0 34 163657.0 35 203771.0 36 252707.0 37 270976.0 38 158275.0 39 14239.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.9444997432969 19.702276799279137 14.31696021625926 24.036263241164697 2 15.938019453526378 20.80622068599167 39.02288123206715 24.232878628414806 3 19.347771319808786 24.150499484357628 30.2598533784396 26.24187581739399 4 13.208870405966678 17.02305069476816 37.008752036118516 32.75932686314665 5 13.36269883253809 37.2810026868908 35.05910636002242 14.29719212054869 6 32.4203123084997 36.67288895313697 17.062211341950256 13.844587396413072 7 28.21884804139802 32.474610715090066 20.985140917732956 18.321400325778963 8 26.06844500777784 35.80567442006631 19.614250472693755 18.5116300994621 9 25.71873019531025 16.5040565242213 20.086053832092894 37.69115944837556 10-11 24.791543364741496 26.528935142750893 27.87861329199118 20.800908200516428 12-13 25.627831579608962 23.69025858050459 28.049621059357765 22.632288780528683 14-15 22.14953882897652 25.47460547748616 26.005960393239302 26.36989530029802 16-17 22.071104998216587 28.599468590203227 26.85138386003799 22.47804255154219 18-19 22.03059284438555 27.289801861444424 28.80525017415033 21.874355120019693 20-21 22.578262896290205 26.510524965038996 28.971491721967034 21.939720416703768 22-23 22.186280620341805 26.53173726083791 28.89792957517795 22.384052543642337 24-25 22.174792734593957 26.70702945161677 28.941997111980672 22.1761807018086 26-27 22.011084905536848 26.964828837095776 28.659173723054394 22.364912534312978 28-29 21.982095227620015 27.353212684969495 28.57936355172861 22.085328535681878 30-31 22.171687729834172 26.981359764557627 28.46824707202854 22.37870543357966 32-33 21.901146121299668 26.936102768562275 28.527536347266057 22.635214762871996 34-35 22.469471282525642 27.009154686866548 28.441309542391362 22.080064488216443 36-37 22.541406483476727 26.674793295269257 28.458745563122932 22.325054658131087 38-39 22.070982821933757 26.891263598243004 28.56754906979774 22.470204510025496 40-41 22.37779305260851 26.797549595575266 28.671267990963035 22.153389360853197 42-43 22.16714029550245 26.812888646540063 28.401004582490064 22.618966475467424 44-45 22.224199931948142 26.91726142136105 28.538578534095922 22.319960112594888 46-47 22.529898309705203 27.117088661512472 27.991423362686646 22.36158966609568 48-49 22.215823438299896 27.17036950050079 27.99800574632349 22.615801314875828 50-51 22.20601430089119 27.315965606354066 28.041259164137838 22.436760928616906 52-53 22.505481177403556 27.057151527889665 27.979163401721163 22.458203892985612 54-55 22.020740663098522 27.186358067960718 28.105838961787654 22.687062307153106 56-57 22.11155785529261 27.304487421304565 27.909504438799885 22.67445028460294 58-59 22.057977695245178 27.005044499504976 27.940005552616277 22.99697225263356 60-61 21.748280008286656 27.207624980128003 28.185261748573343 22.858833263012 62-63 21.85290744707346 27.13289910861807 28.144277276687706 22.86991616762076 64-65 21.646977112907056 27.21408942546695 28.037192999180537 23.10174046244546 66-67 21.436577269669066 27.18742417823966 28.14996872045001 23.226029831641267 68-69 21.694233242423685 26.82837538233337 28.42003939710334 23.057351978139604 70-71 21.597569733047703 26.824337063069827 28.08971982669649 23.48837337718598 72-73 21.618284511264623 26.919414791109837 28.014536554828112 23.447764142797432 74-75 21.92230344882868 27.148160246615465 27.413757016807853 23.515779287747996 76-77 21.856347269263143 27.00443218680833 27.548193056720937 23.59102748720759 78-79 22.01012173953125 27.072327172502224 27.39686814815746 23.520682939809067 80-81 22.157906177081397 27.017522725070513 27.257461758243483 23.567109339604606 82-83 22.006077721933224 27.02980933006786 27.347234646995606 23.616878301003315 84-85 21.945611417192307 27.05151364847446 27.22553385838814 23.777341075945092 86-87 22.114390213306777 27.027142397350158 27.072657759308587 23.785809630034482 88-89 21.982577561382357 26.98479831474151 26.88669758262617 24.14592654124997 90-91 21.89425244251094 26.972857730595894 27.004283609125974 24.128606217767196 92-93 21.981370392815073 26.637829395161056 27.242605484232037 24.138194727791834 94-95 21.593703060799523 26.452826816087576 27.5827355212674 24.370734601845502 96-97 21.93155464894373 26.091614242257087 27.51004615652988 24.466784952269297 98-99 21.78240539444693 26.387324600325 27.08808513268718 24.742184872540896 100-101 21.664112648818136 26.139715732706296 27.392856310370917 24.803315308104647 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 223.0 1 157.0 2 118.5 3 169.0 4 170.5 5 153.5 6 242.0 7 544.0 8 865.5 9 1007.0 10 978.5 11 920.0 12 954.0 13 1030.0 14 1159.0 15 1306.0 16 1476.5 17 1622.5 18 1814.0 19 2143.0 20 2563.0 21 3118.5 22 3914.0 23 5059.0 24 6474.5 25 8087.5 26 10483.5 27 13394.0 28 16293.5 29 19497.5 30 22927.5 31 27255.0 32 33006.5 33 36114.0 34 37418.0 35 40759.0 36 44807.0 37 48533.0 38 51024.0 39 53102.5 40 56039.0 41 59282.0 42 61554.0 43 63406.5 44 65327.0 45 68429.5 46 71133.5 47 72983.0 48 74294.5 49 73032.0 50 71323.5 51 69903.5 52 68630.0 53 68518.0 54 67508.0 55 62947.0 56 57458.5 57 51925.5 58 46730.0 59 40422.5 60 32702.0 61 27050.0 62 22065.0 63 16202.0 64 11044.0 65 7778.5 66 5682.0 67 4380.0 68 3235.5 69 2221.5 70 1568.0 71 1067.0 72 752.5 73 585.5 74 424.5 75 294.5 76 216.5 77 141.5 78 85.0 79 51.0 80 33.0 81 21.5 82 14.5 83 11.5 84 7.5 85 5.0 86 4.0 87 3.5 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023569217554353233 2 0.021631304110995304 3 5.2376039009673856E-5 4 0.0 5 0.0 6 2.0950415603869542E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.713843510870647E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.5712811702902157E-4 26-27 3.6663227306771697E-4 28-29 1.3094009752418463E-4 30-31 6.808885071257602E-4 32-33 0.002514049872464345 34-35 0.004399587276812603 36-37 0.10265703645896075 38-39 0.09472206654899518 40-41 0.10438544574627999 42-43 0.10527583840944443 44-45 0.09956685015738999 46-47 0.09231276875455018 48-49 0.09372692180781136 50-51 0.09252227291058887 52-53 0.0721218057163209 54-55 0.06193466612893934 56-57 0.03383492120024931 58-59 0.013617770142515202 60-61 0.009244370885207435 62-63 0.0022783576969208127 64-65 0.004897159647404505 66-67 0.1609253798572229 68-69 0.4250315565635033 70-71 0.4059404903444772 72-73 0.18810854410324365 74-75 0.028780633435815786 76-77 0.03239458012748328 78-79 0.0058661163690834715 80-81 0.0018331613653385847 82-83 1.0475207801934771E-4 84-85 4.713843510870647E-4 86-87 0.0012832129557370094 88-89 4.1900831207739085E-4 90-91 8.118286046499447E-4 92-93 0.002671177989493367 94-95 0.08115667244548963 96-97 0.017336468912202044 98-99 0.018017357419327806 100-101 0.009768131275304175 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1909270.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.055034209746594 #Duplication Level Percentage of deduplicated Percentage of total 1 78.26400759061052 48.566756684272036 2 12.848690795105453 15.946518936814499 3 4.077875874440822 7.591581806745766 4 1.7975075694689968 4.461775748627082 5 0.9006836479718761 2.7945977293527062 6 0.5335678680628941 1.9866343383518674 7 0.3388435024180559 1.471886159401197 8 0.2244673897013648 1.1143465237512569 9 0.16937996827846644 0.9459791753369426 >10 0.7790174465274843 8.374575044736241 >50 0.04125639932608838 1.7429524347413243 >100 0.021065426439551976 2.5488977703572684 >500 0.0023679675849463994 1.0459810830262457 >1k 0.001268554063364141 1.4075165644856698 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4165 0.21814620247529162 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3674 0.19242956732154173 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2468 0.12926406427587508 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.2376039009673856E-5 2 0.0 0.0 0.0 0.0 5.2376039009673856E-5 3 0.0 0.0 0.0 0.0 5.2376039009673856E-5 4 0.0 0.0 0.0 0.0 5.2376039009673856E-5 5 0.0 0.0 0.0 0.0 5.2376039009673856E-5 6 0.0 0.0 0.0 0.0 5.2376039009673856E-5 7 0.0 0.0 0.0 0.0 5.2376039009673856E-5 8 0.0 0.0 0.0 0.0 5.2376039009673856E-5 9 0.0 0.0 0.0 0.0 5.2376039009673856E-5 10-11 0.0 0.0 0.0 5.2376039009673856E-5 1.3094009752418465E-4 12-13 0.0 0.0 0.0 5.2376039009673856E-5 4.4519633158222774E-4 14-15 0.0 0.0 0.0 5.2376039009673856E-5 5.499484096015755E-4 16-17 0.0 0.0 0.0 5.2376039009673856E-5 5.761364291064124E-4 18-19 0.0 0.0 0.0 5.2376039009673856E-5 5.761364291064124E-4 20-21 0.0 0.0 0.0 7.856405851451078E-5 7.332645461354339E-4 22-23 0.0 0.0 0.0 1.5712811702902157E-4 7.856405851451078E-4 24-25 0.0 0.0 0.0 5.237603900967385E-4 8.380166241547817E-4 26-27 0.0 0.0 0.0 0.0012308369167273357 8.642046436596186E-4 28-29 0.0 0.0 0.0 0.00665175695422858 8.903926631644555E-4 30-31 0.0 0.0 0.0 0.026554651777904642 8.903926631644555E-4 32-33 0.0 0.0 0.0 0.0731169504575047 0.001047520780193477 34-35 0.0 0.0 0.0 0.14542207231035947 0.0011522728582128248 36-37 0.0 0.0 0.0 0.2614873747557967 0.0011522728582128248 38-39 0.0 0.0 0.0 0.4368685413796896 0.0011784608777176617 40-41 0.0 0.0 0.0 0.6269149989262912 0.0012046488972224986 42-43 0.0 0.0 0.0 0.8182970454676395 0.0012046488972224986 44-45 0.0 0.0 0.0 1.0500086420464365 0.0012570249362321723 46-47 0.0 0.0 0.0 1.3262136837639518 0.0013617770142515203 48-49 0.0 0.0 0.0 1.618105349164864 0.0014665290922708679 50-51 0.0 0.0 0.0 1.9306855499745976 0.0015189051312805419 52-53 0.0 0.0 0.0 2.225903093852624 0.0015974691897950525 54-55 0.0 0.0 0.0 2.5351574161852435 0.0016760332483095634 56-57 0.0 0.0 0.0 2.8829605032289827 0.0017284092873192372 58-59 0.0 0.0 0.0 3.2446432406102854 0.001780785326328911 60-61 0.0 0.0 0.0 3.6111183855609736 0.0018855374043482587 62-63 0.0 0.0 0.0 3.9583715241951114 0.0019902894823676065 64-65 0.0 0.0 0.0 4.316833135177319 0.0019902894823676065 66-67 0.0 0.0 0.0 4.704939584239002 0.0020164775018724434 68-69 0.0 0.0 0.0 5.129866388724487 0.0020426655213772803 70-71 0.0 0.0 0.0 5.600281783089872 0.0021474175993966282 72-73 0.0 0.0 0.0 6.090285816044876 0.0023569217554353233 74-75 0.0 0.0 0.0 6.584584684198672 0.002487861852959508 76-77 0.0 0.0 0.0 7.113111293845291 0.002514049872464345 78-79 0.0 0.0 0.0 7.69794214542731 0.002540237891969182 80-81 0.0 0.0 0.0 8.315010449019782 0.002566425911474019 82-83 0.0 0.0 0.0 8.931162171929586 0.0026711779894933664 84-85 0.0 0.0 0.0 9.527489564074227 0.0026711779894933664 86-87 0.0 0.0 0.0 10.132013806323883 0.0026973660089982037 88-89 0.0 0.0 0.0 10.772808455587738 0.0027497420480078775 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3390 0.0 39.61512 1 GTATCAA 5730 0.0 36.853577 1 ATCAACG 6840 0.0 29.821352 3 TCAACGC 6965 0.0 29.286152 4 CAACGCA 7035 0.0 28.994747 5 TATCAAC 7145 0.0 28.952776 2 AACGCAG 7370 0.0 27.805532 6 ACGCAGA 8290 0.0 24.319204 7 CGCAGAG 8320 0.0 24.231516 8 TACATGG 5410 0.0 23.679497 2 GTACATG 5720 0.0 23.146343 1 GTACGGG 150 3.0572766E-5 22.145271 1 ACATGGG 5535 0.0 22.025738 3 GCAGAGT 9370 0.0 20.95925 9 GAGTACT 5140 0.0 20.765661 12-13 AGAGTAC 7685 0.0 20.524574 10-11 GTAGGAC 440 0.0 19.405947 3 GTACTTT 5440 0.0 19.315287 14-15 CTATACT 700 0.0 18.974703 4 CCGATCG 150 7.7931286E-4 18.9747 9 >>END_MODULE