FastQCFastQC Report
Fri 27 May 2016
SRR939803_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939803_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2294867
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56340.24550442356790175No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43520.1896406196960434No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26930.11734884853893494No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39900.046.4921151
GTATCAA73800.039.021841
ATCAACG88150.033.9088173
TATCAAC91300.033.1012232
TCAACGC90100.032.9650234
CAACGCA92000.032.181085
AACGCAG94650.031.3803356
ACGCAGA107100.027.200877
CGCAGAG107850.026.7465848
TACATGG83000.026.6940352
GTACATG84800.025.9036251
ACATGGG85650.024.484393
GCAGAGT116850.023.5907549
GAGTACT62300.022.46637712-13
GTATACG1302.9665796E-421.8969351
CATGGGA55650.021.485194
CAGAGTA112050.019.89929810-11
AGAGTAC104850.019.5916610-11
GTACTTT72500.019.24203914-15
GAGTACA68400.018.8663641
Produced by FastQC (version 0.11.5)