##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939803_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2294867 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.982696600718036 31.0 30.0 33.0 16.0 34.0 2 29.83784289024157 31.0 30.0 33.0 25.0 34.0 3 30.109266898691732 31.0 30.0 34.0 26.0 34.0 4 33.88932604808906 35.0 35.0 37.0 28.0 37.0 5 33.860845530481726 35.0 35.0 37.0 28.0 37.0 6 33.89088605134851 35.0 35.0 37.0 28.0 37.0 7 33.810048251162264 35.0 35.0 37.0 28.0 37.0 8 33.679841576875695 35.0 33.0 37.0 28.0 37.0 9 34.96374168960554 37.0 34.0 39.0 28.0 39.0 10-11 34.809272389206 37.0 34.0 39.0 27.0 39.0 12-13 34.70193937164986 37.0 34.0 39.0 27.0 39.0 14-15 35.410827512008325 38.0 34.0 40.0 27.0 41.0 16-17 35.245551485118746 38.0 33.5 40.0 27.0 41.0 18-19 35.09536761825413 38.0 33.5 40.0 26.0 41.0 20-21 35.28327284326281 38.0 34.0 40.0 27.0 41.0 22-23 35.18789563839647 38.0 34.0 40.0 26.0 41.0 24-25 34.99292268353678 38.0 34.0 40.0 25.0 41.0 26-27 34.89852200585045 38.0 33.5 40.0 25.0 41.0 28-29 34.952438202301046 38.0 34.0 40.0 25.0 41.0 30-31 34.751793894809595 38.0 33.5 40.0 24.5 41.0 32-33 34.42590267758437 38.0 33.0 40.0 24.0 41.0 34-35 34.26146504350797 38.0 33.0 40.0 24.0 41.0 36-37 34.06874123859901 38.0 33.0 40.0 23.5 41.0 38-39 33.858310960940216 38.0 33.0 40.0 22.0 41.0 40-41 33.64465130223233 38.0 32.0 40.0 20.5 41.0 42-43 33.28134070514762 37.5 31.0 40.0 18.0 41.0 44-45 32.958857310685104 37.0 31.0 40.0 16.5 41.0 46-47 32.60803741567594 37.0 31.0 40.0 14.5 41.0 48-49 32.27913251617632 37.0 30.0 40.0 11.5 41.0 50-51 31.667178969413044 36.0 30.0 38.5 9.0 40.5 52-53 31.82700718603736 36.5 30.0 39.0 9.0 40.0 54-55 31.949642397576852 37.0 30.0 39.0 8.5 40.0 56-57 31.67114129054102 36.0 30.0 39.0 7.5 40.0 58-59 31.29104845727443 36.0 29.5 39.0 6.5 40.0 60-61 30.896939125448228 35.5 29.0 39.0 2.0 40.0 62-63 30.46436285850117 35.0 28.0 38.5 2.0 40.0 64-65 29.921450131968435 34.5 28.0 38.0 2.0 40.0 66-67 29.33386161376672 34.0 26.5 38.0 2.0 40.0 68-69 28.790396349766674 34.0 26.0 37.5 2.0 39.5 70-71 28.21293848401672 33.5 26.0 36.5 2.0 39.0 72-73 27.554367856612167 33.0 25.0 36.0 2.0 39.0 74-75 27.190341967530145 32.5 24.5 36.0 2.0 38.5 76-77 26.6070247208226 32.0 22.5 35.0 2.0 38.0 78-79 25.940380858672857 32.0 20.5 35.0 2.0 37.0 80-81 25.198202771663894 31.0 18.5 35.0 2.0 36.5 82-83 24.442136951727484 31.0 15.0 34.0 2.0 36.0 84-85 23.654061869380666 30.0 7.5 34.0 2.0 35.5 86-87 22.706381459143383 29.5 2.0 34.0 2.0 35.0 88-89 21.688564086720497 29.0 2.0 34.0 2.0 35.0 90-91 20.252832517091406 27.5 2.0 33.5 2.0 35.0 92-93 18.65260274342696 24.5 2.0 32.0 2.0 34.0 94-95 18.16108580584409 23.5 2.0 32.0 2.0 34.0 96-97 17.865565629729304 24.0 2.0 32.0 2.0 34.0 98-99 16.966339879391704 19.5 2.0 32.0 2.0 34.0 100-101 15.318385553498306 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.672265810916116 1101 2 1.0461257983037449 1101 3 1.0234645618427898 1101 4 0.9585540164603898 1101 5 0.8512729319511223 1101 6 0.9374126973614167 1101 7 0.9501454119574078 1101 8 0.9472836130694233 1101 9 1.0330656269781926 1101 10-11 1.0669827088497712 1101 12-13 0.9970312330070854 1101 14-15 1.3609773829179446 1101 16-17 1.1204398420853465 1101 18-19 1.0111670611811405 1101 20-21 1.0738861416374021 1101 22-23 1.3703577207764255 1101 24-25 1.3756017037582282 1101 26-27 1.3149066429408052 1101 28-29 1.4303329167582461 1101 30-31 1.3388230434255561 1101 32-33 1.4423236386711835 1101 34-35 1.4808369077868377 1101 36-37 1.1779555861071742 1101 38-39 1.381138504316084 1101 40-41 1.439319085133981 1101 42-43 1.4966469555948265 1101 44-45 1.5087778540135588 1101 46-47 1.4185570905946108 1101 48-49 1.3827368529629567 1101 50-51 1.284221907820836 1101 52-53 1.2623734207574238 1101 54-55 1.3060724816350557 1101 56-57 1.2668672493168778 1101 58-59 1.099714051247691 1101 60-61 0.6243703626703727 1101 62-63 0.5089398149793567 1101 64-65 0.6491257482153188 1101 66-67 0.718310988895766 1101 68-69 0.5767288768530854 1101 70-71 0.7134457987398655 1101 72-73 0.5019601865536707 1101 74-75 0.49394021166613555 1101 76-77 0.3633456223464897 1101 78-79 0.11286091019410804 1101 80-81 -0.08309109205055876 1101 82-83 -0.0262031163541927 1101 84-85 0.0823187925895894 1101 86-87 -0.3456164113668123 1101 88-89 -4.628100405123224 1101 90-91 -8.582445039190503 1101 92-93 -10.886624270362132 1101 94-95 -9.084094611309315 1101 96-97 -5.590294041899595 1101 98-99 -4.212529346596021 1101 100-101 -3.172325269649466 1102 1 1.7199169204164768 1102 2 1.0785604120392733 1102 3 0.8950138561759253 1102 4 0.9635343127633504 1102 5 0.8095901364349629 1102 6 0.8341887916467385 1102 7 0.77290973410161 1102 8 1.045884707235949 1102 9 1.325508260361488 1102 10-11 1.2048404174894571 1102 12-13 1.08903128710498 1102 14-15 1.3062087446387878 1102 16-17 1.1983274727107442 1102 18-19 1.2200717578655684 1102 20-21 1.1903108481471705 1102 22-23 1.1916010878441838 1102 24-25 1.3692773671245604 1102 26-27 1.3569439990011034 1102 28-29 1.1606123141222966 1102 30-31 1.202037606287135 1102 32-33 1.4448256664285282 1102 34-35 1.5657684136347072 1102 36-37 1.4116232298257358 1102 38-39 1.468415592650274 1102 40-41 1.3299956414811405 1102 42-43 1.3514840204037384 1102 44-45 1.2969240908651187 1102 46-47 1.3246246815311693 1102 48-49 1.141483989521042 1102 50-51 1.3230652947732224 1102 52-53 1.2250261330907612 1102 54-55 1.2349732233245447 1102 56-57 1.105822560432788 1102 58-59 1.2058981719795803 1102 60-61 1.176872737677737 1102 62-63 1.1237856304887153 1102 64-65 1.1143051613335437 1102 66-67 0.8953642691108108 1102 68-69 0.9275601385659193 1102 70-71 0.8596380560879489 1102 72-73 0.8809364277074785 1102 74-75 0.665646207138721 1102 76-77 0.6954575494342201 1102 78-79 0.7361222384948789 1102 80-81 0.6641368476127418 1102 82-83 0.43712472311834105 1102 84-85 0.33930025419050125 1102 86-87 0.3847812337663328 1102 88-89 -0.19459805241297445 1102 90-91 -5.93178458793362 1102 92-93 -10.914459100708568 1102 94-95 -8.72412464295293 1102 96-97 -5.116007540165722 1102 98-99 -3.4591845064936884 1102 100-101 -2.654955641013256 1103 1 2.1457510762179055 1103 2 1.324969545186761 1103 3 1.0876682060221121 1103 4 0.9452974534948524 1103 5 0.9759128094274132 1103 6 0.873208409675243 1103 7 0.9360625861901042 1103 8 0.9621251334187164 1103 9 0.945123980179801 1103 10-11 0.9838922993541885 1103 12-13 0.8340563651673065 1103 14-15 0.9969976791241564 1103 16-17 1.0375548721532013 1103 18-19 1.0437651879326921 1103 20-21 0.8205479498273149 1103 22-23 0.8813081160505121 1103 24-25 0.8196095288015854 1103 26-27 0.8011304481898947 1103 28-29 0.7649387848972609 1103 30-31 0.9063179034145534 1103 32-33 0.8606531270510587 1103 34-35 0.9435435941448134 1103 36-37 0.997185018823707 1103 38-39 0.8422813081398033 1103 40-41 0.6188299877488674 1103 42-43 0.7468031668838719 1103 44-45 0.6751563904859239 1103 46-47 0.6785800316139969 1103 48-49 0.6849873573114564 1103 50-51 0.5416288325980929 1103 52-53 0.5487775688026097 1103 54-55 0.4422158485272689 1103 56-57 0.47227769407664155 1103 58-59 0.6713360424509567 1103 60-61 0.7792489490145122 1103 62-63 0.508816422539919 1103 64-65 0.552339260572257 1103 66-67 0.6550428428068393 1103 68-69 0.43700986412130405 1103 70-71 0.2622895777982208 1103 72-73 0.5492667886122469 1103 74-75 0.3232484096217121 1103 76-77 0.07698635890443839 1103 78-79 -0.04783674776269109 1103 80-81 -0.22409550972511738 1103 82-83 -0.6535949508957088 1103 84-85 -0.45506252061484886 1103 86-87 -0.32057144632532086 1103 88-89 -1.0406037433150317 1103 90-91 -6.697743932036957 1103 92-93 -10.035317468816428 1103 94-95 -8.792917894674343 1103 96-97 -5.453547869961 1103 98-99 -3.8064195707616335 1103 100-101 -2.961999719531544 1104 1 1.9900277153165504 1104 2 1.2628176413951593 1104 3 0.962694175897056 1104 4 0.9158496129898381 1104 5 0.8777356948802932 1104 6 0.8143866429246742 1104 7 0.8683131755065006 1104 8 0.8505235912076827 1104 9 1.0628174557481387 1104 10-11 0.9924124160188583 1104 12-13 0.8306158561577561 1104 14-15 1.095692193487487 1104 16-17 1.0658240798722503 1104 18-19 1.0069458983086577 1104 20-21 0.987052304775581 1104 22-23 0.973365829103372 1104 24-25 1.1407929560260044 1104 26-27 1.072596048094006 1104 28-29 0.8293390805542984 1104 30-31 0.9926492590420324 1104 32-33 0.9178352957353866 1104 34-35 1.0121609979563857 1104 36-37 0.9935577264828197 1104 38-39 0.7076884358716313 1104 40-41 0.5786331161199527 1104 42-43 0.7444019522927974 1104 44-45 0.6021700546355078 1104 46-47 0.482854073725143 1104 48-49 0.39693749515156895 1104 50-51 0.3155695613527385 1104 52-53 0.4848202592816584 1104 54-55 0.4504527936709053 1104 56-57 0.6824204285047024 1104 58-59 0.5692274883736879 1104 60-61 0.5136380576484996 1104 62-63 0.604058941218252 1104 64-65 0.6216573515866806 1104 66-67 0.5907738947865404 1104 68-69 0.5863191653318971 1104 70-71 0.4956988230737025 1104 72-73 0.2802113928713119 1104 74-75 0.01670400113555459 1104 76-77 0.25792946490147983 1104 78-79 0.02003502098555998 1104 80-81 -0.19940664880378733 1104 82-83 -0.25899268951890875 1104 84-85 -0.3147153233636608 1104 86-87 0.04170880857898851 1104 88-89 -0.4513108231024816 1104 90-91 -3.545279291020538 1104 92-93 -8.402824161053434 1104 94-95 -6.080007870302612 1104 96-97 -3.573040758210629 1104 98-99 -2.6734073649204095 1104 100-101 -2.198888928049664 1105 1 1.942456125117598 1105 2 1.2600306849503724 1105 3 1.0019619088774938 1105 4 0.8759537256226366 1105 5 0.9032599340493519 1105 6 0.9337448260523544 1105 7 0.8708468960456983 1105 8 0.942849011599705 1105 9 1.0024618771381313 1105 10-11 0.984505601386175 1105 12-13 0.8763491885380503 1105 14-15 0.9150660677109315 1105 16-17 0.6379142835910017 1105 18-19 0.8060096742738452 1105 20-21 0.7931250821880198 1105 22-23 0.7759629096745968 1105 24-25 0.8636609751902498 1105 26-27 0.7550255436780375 1105 28-29 0.7734081526897683 1105 30-31 0.9615166444357612 1105 32-33 1.0250281995479469 1105 34-35 1.0522793176680807 1105 36-37 0.9427069185105239 1105 38-39 0.9223223738397266 1105 40-41 0.7980918958496517 1105 42-43 0.9539730441888352 1105 44-45 0.842693279499457 1105 46-47 0.8762354606137919 1105 48-49 0.7095745795077093 1105 50-51 0.6771025337568375 1105 52-53 0.6689213160650347 1105 54-55 0.547498902347229 1105 56-57 0.8153566001224029 1105 58-59 0.7137249512494357 1105 60-61 0.6008054983184579 1105 62-63 0.6891177845292162 1105 64-65 0.7210615494483363 1105 66-67 0.7432218248553113 1105 68-69 0.6990958527884175 1105 70-71 0.6211418157230533 1105 72-73 0.6625103902749601 1105 74-75 0.6791514308591857 1105 76-77 0.5152394392647501 1105 78-79 0.25526352214214043 1105 80-81 0.12169213974831905 1105 82-83 -0.26411050548175297 1105 84-85 0.01364825532258962 1105 86-87 0.18457700133235733 1105 88-89 0.01854142531922065 1105 90-91 -3.2047085674057456 1105 92-93 -8.367396151528496 1105 94-95 -5.6246698003686575 1105 96-97 -2.9185700042347573 1105 98-99 -1.9760434179529085 1105 100-101 -1.4635044519552682 1106 1 1.6449947453705605 1106 2 0.9216601588189732 1106 3 0.6095526426012761 1106 4 0.6962763998283705 1106 5 0.6440591317072233 1106 6 0.7351358946118296 1106 7 0.6875555846872743 1106 8 -0.2822922741291407 1106 9 -0.5399513017764122 1106 10-11 -0.7379436814882396 1106 12-13 -0.33313418526177685 1106 14-15 0.36876080079721163 1106 16-17 0.5229907631067263 1106 18-19 0.5951170327619764 1106 20-21 0.488662143846895 1106 22-23 0.5758805468430808 1106 24-25 0.642876358391888 1106 26-27 0.7741779980150341 1106 28-29 0.5376006200353984 1106 30-31 0.5066007374431223 1106 32-33 0.549194574262394 1106 34-35 0.7000392963316386 1106 36-37 0.5371262399082752 1106 38-39 0.5376267483847457 1106 40-41 0.5268172839187528 1106 42-43 0.44708635204096225 1106 44-45 0.3823552275482385 1106 46-47 0.3252541422054378 1106 48-49 0.36591633805556967 1106 50-51 0.26224674858006125 1106 52-53 0.32800936981105266 1106 54-55 0.34222287109781746 1106 56-57 0.3024452526097612 1106 58-59 0.3463773000528896 1106 60-61 0.17988431608428002 1106 62-63 0.2789204619224961 1106 64-65 0.07784032098041038 1106 66-67 0.161778014649137 1106 68-69 0.18239866873934574 1106 70-71 0.279964510030414 1106 72-73 0.3231171938145714 1106 74-75 0.3190216999769149 1106 76-77 0.1263824178378492 1106 78-79 0.26582127411963796 1106 80-81 0.047594577214304934 1106 82-83 -0.28836949126846534 1106 84-85 -0.4429819436404543 1106 86-87 -0.3395041209415375 1106 88-89 -0.6440386581312545 1106 90-91 -3.7406372662129996 1106 92-93 -8.794845516963173 1106 94-95 -6.102370274623498 1106 96-97 -3.2722721497963896 1106 98-99 -2.2344763201541813 1106 100-101 -1.5290122477973913 1107 1 1.6727251207971818 1107 2 0.8871038448257984 1107 3 0.7324195367992274 1107 4 0.6723856148454317 1107 5 0.5083936026969837 1107 6 0.5260915260111432 1107 7 0.5749275983391513 1107 8 0.4621616507855322 1107 9 0.5466527937867269 1107 10-11 0.6234044413786464 1107 12-13 0.41238629255392567 1107 14-15 0.6513973195685381 1107 16-17 0.5338696026971661 1107 18-19 0.5616272716698205 1107 20-21 0.6059829629595228 1107 22-23 0.4278430041809642 1107 24-25 0.41932687033727944 1107 26-27 0.3865926737829497 1107 28-29 0.17497263368726834 1107 30-31 0.5381707033134333 1107 32-33 0.6481706834774172 1107 34-35 0.6627099447958074 1107 36-37 0.35389245151236537 1107 38-39 0.23920524667826015 1107 40-41 0.34081045798019005 1107 42-43 0.402931061938574 1107 44-45 0.10206512515915733 1107 46-47 -0.009003536975448867 1107 48-49 0.16689756672450784 1107 50-51 0.35716995677118746 1107 52-53 0.2096219977973952 1107 54-55 0.4222143386746069 1107 56-57 0.25935651540839544 1107 58-59 0.2437749109743912 1107 60-61 0.10266588605014704 1107 62-63 0.15093411379962873 1107 64-65 0.18086933121931992 1107 66-67 0.28315173478565825 1107 68-69 0.3931495219816661 1107 70-71 0.43738771822154376 1107 72-73 0.3546871596848753 1107 74-75 0.19658142693937108 1107 76-77 0.1199831004317069 1107 78-79 -0.12240398185306134 1107 80-81 -0.00871941595975656 1107 82-83 0.033729484840748114 1107 84-85 0.09989348298070766 1107 86-87 0.2925351282052233 1107 88-89 -0.029277565981082887 1107 90-91 -3.4995963105816017 1107 92-93 -4.7193335715706795 1107 94-95 -3.2009197626781063 1107 96-97 -1.7176646412639656 1107 98-99 -1.2506879242497444 1107 100-101 -0.9057187324390288 1108 1 1.62262510818735 1108 2 0.9485982625494067 1108 3 -0.38046861397558374 1108 4 -0.19198419139269163 1108 5 0.3702161532367825 1108 6 0.4893420016513019 1108 7 0.283802219178078 1108 8 0.4538405462195243 1108 9 0.5230000020942569 1108 10-11 0.711842505616751 1108 12-13 0.4917602593443391 1108 14-15 0.5170284556037004 1108 16-17 0.4284166637052067 1108 18-19 0.4091677132080065 1108 20-21 0.4156580484223227 1108 22-23 0.3443680700361682 1108 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0.983265953135465 2311 80-81 1.205108633749262 2311 82-83 1.3235353688849862 2311 84-85 1.4868412049168889 2311 86-87 1.7567408807356628 2311 88-89 2.123064457733996 2311 90-91 2.848357837959771 2311 92-93 3.689611429574075 2311 94-95 3.2824945909608836 2311 96-97 2.3474626395951894 2311 98-99 1.9694888606373517 2311 100-101 2.0260112330053257 2312 1 1.2774998701697804 2312 2 0.5514555543839847 2312 3 -0.0526536001658755 2312 4 -0.5534028826195581 2312 5 -0.035181222044236904 2312 6 -0.2731028715877528 2312 7 0.023030821283285263 2312 8 0.05166723795255734 2312 9 0.006468888997083866 2312 10-11 -0.04299685378573059 2312 12-13 -0.012427323777011168 2312 14-15 0.07795968176021262 2312 16-17 -0.20548775282625797 2312 18-19 -0.6609010984703048 2312 20-21 0.13439035309371405 2312 22-23 0.27849335924202023 2312 24-25 0.1939496263166589 2312 26-27 0.21719868296082012 2312 28-29 0.23639806284744935 2312 30-31 0.32472766007803244 2312 32-33 0.31316399518013327 2312 34-35 0.13298644341537624 2312 36-37 0.2989817646690085 2312 38-39 0.3179358933784826 2312 40-41 0.30030566859807806 2312 42-43 0.26316368906967824 2312 44-45 0.22803930472357337 2312 46-47 0.2731889884347325 2312 48-49 0.3661937921461842 2312 50-51 0.2160751139094259 2312 52-53 -0.022284688094501348 2312 54-55 0.004050266256584223 2312 56-57 0.16482935998599757 2312 58-59 0.15909445256210475 2312 60-61 0.13286628774888243 2312 62-63 0.149310314151883 2312 64-65 0.2927645113690396 2312 66-67 0.4255687023477499 2312 68-69 0.4588009060115361 2312 70-71 0.5278997237485719 2312 72-73 0.4100266392137435 2312 74-75 0.48444798037492376 2312 76-77 0.6448176636277623 2312 78-79 0.8331771383064464 2312 80-81 0.7244150168111645 2312 82-83 0.755517197884064 2312 84-85 1.0351521350048323 2312 86-87 1.7739492119819928 2312 88-89 1.9908015243035777 2312 90-91 2.8169079816883063 2312 92-93 3.899014378910529 2312 94-95 3.7398587390898506 2312 96-97 2.9851671610884942 2312 98-99 2.7006931323007777 2312 100-101 2.6595633252847843 2313 1 -7.19732573312637 2313 2 -2.61899930723186 2313 3 -1.4444502730690907 2313 4 -0.7341116295735759 2313 5 -0.4454543328312752 2313 6 -0.09999333498358709 2313 7 -0.00844592764642016 2313 8 0.07837904534794404 2313 9 0.1631866203315937 2313 10-11 -0.09195477193533463 2313 12-13 -0.09471818449879521 2313 14-15 -0.19689108655436627 2313 16-17 -0.29914210426433385 2313 18-19 -0.5227141989725084 2313 20-21 -0.4024640508626618 2313 22-23 -0.9176606131577358 2313 24-25 -0.41255084181521084 2313 26-27 -0.4135808699146395 2313 28-29 -0.35274190310943965 2313 30-31 0.1408710194047984 2313 32-33 -0.20855536953601472 2313 34-35 -0.09922642137969717 2313 36-37 -0.017219505461056883 2313 38-39 -0.04348542998888405 2313 40-41 -0.01773022515865108 2313 42-43 -0.07174245791591005 2313 44-45 -0.08886002113528235 2313 46-47 -1.0814246503800433 2313 48-49 -0.5883511508573918 2313 50-51 -0.2814952895280243 2313 52-53 -0.7606196860703598 2313 54-55 -0.5681621608860006 2313 56-57 -0.4255032871707449 2313 58-59 -0.5593827668440348 2313 60-61 -0.25721392770119067 2313 62-63 -0.12156602244664683 2313 64-65 -0.29231833781122063 2313 66-67 -0.1537630022930827 2313 68-69 -0.13529367694471262 2313 70-71 -0.04347913169636897 2313 72-73 -0.1931804157444681 2313 74-75 0.014310729426547653 2313 76-77 0.1647047492841729 2313 78-79 0.16295524849171272 2313 80-81 0.2492084995403019 2313 82-83 0.46643412675146934 2313 84-85 0.608309500774471 2313 86-87 1.1792979506831323 2313 88-89 1.777269152867909 2313 90-91 2.3654814690127957 2313 92-93 3.3618905842133167 2313 94-95 3.0747849711988486 2313 96-97 2.418646013876266 2313 98-99 2.012732249055521 2313 100-101 1.6447509766070407 2314 1 0.2524305312373336 2314 2 -0.17923421590057842 2314 3 -1.5621407884526661 2314 4 -0.6376432118273954 2314 5 -0.4754389850408032 2314 6 -3.672101707879232 2314 7 -2.9096984027758417 2314 8 -2.664609081983052 2314 9 -3.3255546222827803 2314 10-11 -2.678098420892404 2314 12-13 -1.4700547623391316 2314 14-15 -1.4455670414175543 2314 16-17 -2.3668226793691076 2314 18-19 -3.290046069096803 2314 20-21 -2.9524751839749186 2314 22-23 -4.30791277815046 2314 24-25 -3.5025946585641776 2314 26-27 -2.189895571189382 2314 28-29 -1.9233385584044314 2314 30-31 -2.3362746891780333 2314 32-33 -2.5076702871112246 2314 34-35 -1.9852670890483424 2314 36-37 -2.2888928917561913 2314 38-39 -3.493576085331579 2314 40-41 -2.8411523053011543 2314 42-43 -1.866020435824641 2314 44-45 -2.296431332695427 2314 46-47 -2.0403041026309197 2314 48-49 -2.429817620308924 2314 50-51 -3.5092294626161085 2314 52-53 -2.223762722706251 2314 54-55 -2.663997979289036 2314 56-57 -1.7587332416045065 2314 58-59 -1.1851901162885703 2314 60-61 -1.6449989154945257 2314 62-63 -1.262345022216035 2314 64-65 -1.7511767166710293 2314 66-67 -1.1149422374482754 2314 68-69 -1.170982856354815 2314 70-71 -0.9796283472095659 2314 72-73 -2.2353224265435863 2314 74-75 -2.246066921645813 2314 76-77 -1.3561543293712681 2314 78-79 -2.382257910846981 2314 80-81 -1.0878929946824059 2314 82-83 -0.5572133989143317 2314 84-85 -1.7358340845648108 2314 86-87 -0.31296811479547415 2314 88-89 0.8872806040690904 2314 90-91 2.063918061962351 2314 92-93 3.1700503602787116 2314 94-95 2.779858489500892 2314 96-97 1.781114304386879 2314 98-99 1.7884437742123787 2314 100-101 1.666408458456095 2315 1 -2.80383860846538 2315 2 -1.81291675314473 2315 3 -2.8312918455820686 2315 4 -4.2351302126923365 2315 5 -3.5876072441084688 2315 6 -3.154054533625704 2315 7 -5.661999842448836 2315 8 -3.044074956957587 2315 9 -2.5544896783702526 2315 10-11 -3.9180028434369234 2315 12-13 -3.0728588018391356 2315 14-15 -2.2054811152145675 2315 16-17 -2.5752937406395304 2315 18-19 -2.1086440994398075 2315 20-21 -1.5033588246010154 2315 22-23 -0.9949934579459452 2315 24-25 -2.386927403026398 2315 26-27 -3.954365603883417 2315 28-29 -3.3422209500449114 2315 30-31 -3.220893297727912 2315 32-33 -3.797647177537783 2315 34-35 -3.8163691685759993 2315 36-37 -3.000863784380165 2315 38-39 -2.0147278571212013 2315 40-41 -1.7321945069889537 2315 42-43 -3.0710811652455945 2315 44-45 -6.592550293566113 2315 46-47 -3.4907537708482437 2315 48-49 -2.4558180611273777 2315 50-51 -3.4701859718682897 2315 52-53 -3.3221709339517993 2315 54-55 -1.86835490197549 2315 56-57 -1.4877451069678678 2315 58-59 -2.816977301161817 2315 60-61 -3.19644292988124 2315 62-63 -4.906286941045327 2315 64-65 -4.268017484363046 2315 66-67 -3.5956216989818515 2315 68-69 -2.1592667701547725 2315 70-71 -3.811089426613659 2315 72-73 -2.6107078922248697 2315 74-75 -1.7243983570108057 2315 76-77 -3.473903951169074 2315 78-79 -1.9625503204207533 2315 80-81 -1.3819444502181852 2315 82-83 -1.0515223325010261 2315 84-85 -2.5526085625122015 2315 86-87 -1.814629031898228 2315 88-89 -1.4587593768287768 2315 90-91 -0.42852741180059795 2315 92-93 0.28517277088774406 2315 94-95 0.07231982604908538 2315 96-97 -0.28547416153151417 2315 98-99 -0.638380529578324 2315 100-101 -1.130762288564208 2316 1 -7.584058704950959 2316 2 -6.0294556477696695 2316 3 -3.301054660860032 2316 4 -4.172201972492889 2316 5 -2.0007540338584064 2316 6 -2.9803707775234045 2316 7 -3.2783901593546787 2316 8 -2.3681844799619505 2316 9 -6.974616742899027 2316 10-11 -6.7354227715584045 2316 12-13 -5.960461376226924 2316 14-15 -4.687585234738684 2316 16-17 -4.6202843690909035 2316 18-19 -3.8769610700372468 2316 20-21 -2.564919826007653 2316 22-23 -3.3233890704063285 2316 24-25 -2.996735691000957 2316 26-27 -3.9458785064013213 2316 28-29 -4.382177024283404 2316 30-31 -6.994858380165162 2316 32-33 -4.817033691861319 2316 34-35 -5.33219953095454 2316 36-37 -4.027087751800991 2316 38-39 -6.2467197339132206 2316 40-41 -3.5650994352363483 2316 42-43 -4.11345045822431 2316 44-45 -2.7231348191786786 2316 46-47 -3.9301113970583614 2316 48-49 -4.871257012952942 2316 50-51 -4.457054471593075 2316 52-53 -6.051127939948866 2316 54-55 -4.781176475604205 2316 56-57 -3.9271120867389904 2316 58-59 -2.7266893553836944 2316 60-61 -2.9866511905146176 2316 62-63 -3.95485566633484 2316 64-65 -2.7280657271527105 2316 66-67 -3.8412755308839728 2316 68-69 -2.87036045326332 2316 70-71 -2.395750126188073 2316 72-73 -1.9347359313227521 2316 74-75 -1.8946263320727752 2316 76-77 -2.8117029503380415 2316 78-79 -4.548643014210349 2316 80-81 -5.558145033736491 2316 82-83 -3.004838349754749 2316 84-85 -1.66078321508137 2316 86-87 -1.767451468932574 2316 88-89 -0.7881840608762758 2316 90-91 -0.4242844926261604 2316 92-93 -0.1805284403984082 2316 94-95 -1.0982659227214633 2316 96-97 -2.716692750654758 2316 98-99 -4.260768817480933 2316 100-101 -3.59392274890331 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16471.0 3 5639.0 4 5216.0 5 5785.0 6 6513.0 7 7709.0 8 9207.0 9 11200.0 10 13217.0 11 15617.0 12 18906.0 13 21136.0 14 23375.0 15 25606.0 16 28540.0 17 31048.0 18 29177.0 19 29176.0 20 31716.0 21 34954.0 22 38728.0 23 44337.0 24 49941.0 25 56608.0 26 64351.0 27 72856.0 28 83832.0 29 95454.0 30 107989.0 31 123862.0 32 139425.0 33 155886.0 34 178490.0 35 210887.0 36 234321.0 37 200034.0 38 66227.0 39 1431.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.56519542242075 20.009390085169642 13.385409326117395 23.040005166292215 2 15.288693900219844 20.361424179322096 40.77685998728676 23.573021933171297 3 19.090347844108248 25.06947065303941 30.09832131676829 25.741860186084054 4 12.369422816121144 16.768161246349948 38.37271811734973 32.48969782017918 5 13.138215766295207 37.975763223728585 35.0613030932081 13.824717916768103 6 31.09519157048833 37.04506290846429 18.05775980150659 13.801985719540788 7 27.45201600019555 33.234932596295366 21.699741419799107 17.61330998370997 8 25.76301737249947 35.70948799025865 20.34723176935012 18.180262867891756 9 26.480085691239168 16.511713580350378 20.528097577668596 36.480103150741854 10-11 24.49987189911848 26.963206022443625 28.224857856032067 20.312064222405827 12-13 25.41990387919983 24.27112393691595 28.42683745110768 21.882134732776535 14-15 22.40095509766576 25.6809064262658 26.542082954360964 25.37605552170748 16-17 21.743289668463536 28.89485954389297 27.27287605088959 22.0889747367539 18-19 21.698814464916044 27.698749865235463 29.344579497545432 21.25785617230306 20-21 22.682588806341503 26.898652954682355 29.2511589129353 21.16759932604084 22-23 22.098069888358964 26.9665229717235 29.09740726872992 21.837999871187616 24-25 21.775864678221403 27.037647932854487 29.46467158267545 21.721815806248664 26-27 21.7546327288135 27.26552970199178 29.17569151033129 21.80414605886342 28-29 21.6338871232915 27.51472415040211 29.241743381339337 21.609645344967056 30-31 21.884977711508103 27.312983797195578 29.11870868472675 21.683329806569564 32-33 21.612279156631267 27.233186881484066 29.120238504177685 22.03429545770698 34-35 22.011584205373122 27.392151947601622 28.96070995291378 21.635553894111474 36-37 22.06779669899885 27.048210898605866 29.08686801454708 21.7971243878482 38-39 21.719879080725295 27.34690490980728 29.146283282550005 21.78693272691742 40-41 21.97005807980509 27.238880519890568 29.114710399056015 21.676351001248324 42-43 21.759856559609663 27.186403730463244 28.99331684287037 22.060422867056722 44-45 21.78266457631659 27.286425918361513 29.011445037816458 21.919464467505442 46-47 21.885609374986352 27.39444886494154 28.67174446401885 22.048197296053257 48-49 21.72095667976105 27.50599579578884 28.649298318774274 22.12374920567584 50-51 21.80087218605529 27.612707973208337 28.50763474090552 22.078785099830853 52-53 21.82686957644408 27.601226057054777 28.36590607176117 22.205998294739974 54-55 21.609847644966436 27.584228022027833 28.53892914353896 22.266995189466765 56-57 21.624006174244194 27.736381262199654 28.35374016547638 22.285872398079764 58-59 21.605566477439744 27.501156370564463 28.458508900013147 22.434768251982646 60-61 21.583505646207666 27.432580745801992 28.551015199556538 22.4328984084338 62-63 21.570673107569895 27.357247456952223 28.606067512471828 22.466011923006057 64-65 21.461331957107333 27.392464333064158 28.486939497358776 22.659264212469736 66-67 21.424875466346222 27.514448873083964 28.268004108038618 22.792671552531193 68-69 21.573848430478783 27.306119187461817 28.43187170137244 22.68816068068696 70-71 21.529248548249473 27.12157761112038 28.270794595968376 23.078379244661775 72-73 21.406558845049197 27.13632045079774 28.35963350842516 23.097487195727908 74-75 21.329407072598695 27.34635359673528 28.221309531142087 23.102929799523938 76-77 21.37432045064013 27.126531293458285 28.294419033629804 23.204729222271787 78-79 21.432451956736433 27.146723940162538 28.252997454454658 23.16782664864637 80-81 21.472425878613656 27.060046483032906 28.20907926628643 23.258448372067008 82-83 21.41042250628658 27.04820185222002 28.2478409336692 23.293534707824197 84-85 21.374789418930575 26.987488868935305 28.157023311403563 23.48069840073056 86-87 21.19502527486953 26.882247699618826 28.417262180001458 23.505464845510186 88-89 21.37772663550075 26.73995506411206 28.252795168627532 23.62952313175966 90-91 21.21224854154589 26.73317878326509 28.435155391536686 23.619417283652336 92-93 21.54768106676649 26.41698827651821 28.392036948111905 23.643293708603395 94-95 21.21029066486689 26.30302020620071 28.594940896081305 23.891748232851096 96-97 21.51782731259706 26.141699455630725 28.196353143206505 24.14412008856571 98-99 21.410057896862796 26.148997427777555 28.052699959257115 24.388244716102534 100-101 21.603751516675228 26.104718909524173 28.130231846353965 24.16129772744663 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2862.0 1 1661.5 2 438.5 3 422.5 4 361.5 5 335.5 6 461.5 7 706.5 8 1055.5 9 1280.5 10 1248.5 11 1227.5 12 1332.0 13 1425.5 14 1549.0 15 1729.5 16 1989.0 17 2202.0 18 2430.0 19 2867.0 20 3382.5 21 4119.5 22 5123.0 23 6355.5 24 8021.0 25 10285.5 26 13300.5 27 17082.0 28 20723.0 29 24390.5 30 28899.5 31 34017.5 32 39505.0 33 44052.0 34 48411.5 35 54089.5 36 60731.5 37 66419.5 38 70045.5 39 72521.5 40 75426.5 41 78399.0 42 80283.0 43 82481.0 44 85020.0 45 87599.5 46 88802.0 47 89170.5 48 89716.0 49 88606.0 50 86101.5 51 83041.5 52 79639.5 53 75967.5 54 71627.5 55 65482.5 56 58493.5 57 51636.0 58 45426.5 59 39360.5 60 32374.0 61 26171.5 62 20775.0 63 15376.5 64 10923.5 65 7941.5 66 5882.0 67 4361.5 68 3214.0 69 2329.5 70 1669.5 71 1135.0 72 770.5 73 579.0 74 416.0 75 312.5 76 232.0 77 142.5 78 90.0 79 61.5 80 40.5 81 30.5 82 26.5 83 20.0 84 15.5 85 12.0 86 8.0 87 7.5 88 7.5 89 5.5 90 3.0 91 4.5 92 3.5 93 3.5 94 3.5 95 0.5 96 0.0 97 0.5 98 2.5 99 2.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13456117500491313 2 0.12061701179196878 3 0.1260203750369847 4 0.1359120158161671 5 0.12257790974378906 6 0.1396159341696055 7 0.1697266116075572 8 0.15695898716570503 9 0.16780928916577736 10-11 0.16140368918983106 12-13 0.15162098718574976 14-15 0.15613105247493647 16-17 0.15142489739056775 18-19 0.1670685054950897 20-21 0.17112102792885164 22-23 0.20543674208570695 24-25 0.22975187668827865 26-27 0.24334743582089943 28-29 0.23665859502969017 30-31 0.22800885628666062 32-33 0.22935969709791462 34-35 0.218966937953267 36-37 0.22650550119026505 38-39 0.2139339665435949 40-41 0.21789933795727598 42-43 0.21162446451145098 44-45 0.20308366454352256 46-47 0.21914123999342883 48-49 0.260211158206554 50-51 0.26234635819853613 52-53 0.2643508316603969 54-55 0.2629999908491429 56-57 0.23339043177665633 58-59 0.23426194197746536 60-61 0.20487026045518103 62-63 0.1963076727322324 64-65 0.1980724808888707 66-67 0.20580713392105077 68-69 0.2052406522905249 70-71 0.21234346042711844 72-73 0.2436742521462028 74-75 0.2468988398891962 76-77 0.2629346275840822 78-79 0.25999328065635174 80-81 0.2740899581544377 82-83 0.27286984387330504 84-85 0.24687705213417596 86-87 0.24992733783700755 88-89 0.25395807251574926 90-91 0.4431847248664084 92-93 0.5885090508513129 94-95 0.34513982727539333 96-97 0.2885788152428877 98-99 0.32032357430735636 100-101 0.33954037423519534 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2294867.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.46423979805273 #Duplication Level Percentage of deduplicated Percentage of total 1 75.02839855349941 44.61506683249162 2 13.323348487627614 15.845255787626233 3 5.143414739583777 9.17547742366346 4 2.437784570380756 5.798440250764569 5 1.2816560638254855 3.8106351758973527 6 0.7971592215068555 2.844148026294761 7 0.4976661651806841 2.0715338129977066 8 0.3230704093086377 1.5368909032627103 9 0.2253766348350419 1.2061665232838188 >10 0.8877349009206739 8.756533199222732 >50 0.03552158209902582 1.4458140995243072 >100 0.017677126178656084 1.9318362887047487 >500 8.936587900488257E-4 0.35626438169934427 >1k 2.2341469751197113E-4 0.3564415175010198 >5k 7.447156583732372E-5 0.24949577706556658 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5634 0.24550442356790175 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4352 0.1896406196960434 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2693 0.11734884853893494 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.1787755020225573E-5 0.0 12-13 0.0 0.0 0.0 6.536326506067673E-5 4.3575510040451146E-5 14-15 0.0 4.3575510040451146E-5 0.0 1.0893877510112786E-4 4.3575510040451146E-5 16-17 0.0 4.3575510040451146E-5 0.0 2.1787755020225572E-4 4.3575510040451146E-5 18-19 0.0 4.3575510040451146E-5 0.0 4.5754285542473703E-4 4.3575510040451146E-5 20-21 0.0 4.3575510040451146E-5 0.0 5.882693855460905E-4 4.3575510040451146E-5 22-23 0.0 4.3575510040451146E-5 0.0 9.150857108494741E-4 4.3575510040451146E-5 24-25 4.3575510040451146E-5 4.3575510040451146E-5 0.0 0.0015687183614562414 4.3575510040451146E-5 26-27 4.3575510040451146E-5 4.3575510040451146E-5 0.0 0.0027888326425888734 8.715102008090229E-5 28-29 4.3575510040451146E-5 4.3575510040451146E-5 0.0 0.008366497927766621 8.715102008090229E-5 30-31 4.3575510040451146E-5 4.3575510040451146E-5 0.0 0.029674922337547233 8.715102008090229E-5 32-33 4.3575510040451146E-5 4.3575510040451146E-5 0.0 0.080266089494511 8.715102008090229E-5 34-35 4.3575510040451146E-5 4.3575510040451146E-5 0.0 0.15698077492072526 8.715102008090229E-5 36-37 4.3575510040451146E-5 4.3575510040451146E-5 0.0 0.2872061866766135 8.715102008090229E-5 38-39 4.3575510040451146E-5 4.3575510040451146E-5 0.0 0.48760995735264834 8.715102008090229E-5 40-41 4.3575510040451146E-5 4.3575510040451146E-5 0.0 0.705291417759722 8.715102008090229E-5 42-43 4.3575510040451146E-5 4.3575510040451146E-5 0.0 0.9224063965362699 8.715102008090229E-5 44-45 4.3575510040451146E-5 4.3575510040451146E-5 0.0 1.187933766967759 1.0893877510112786E-4 46-47 4.3575510040451146E-5 4.3575510040451146E-5 0.0 1.5027886147650387 1.52514285141579E-4 48-49 4.3575510040451146E-5 4.3575510040451146E-5 0.0 1.8342893073977709 1.7430204016180459E-4 50-51 4.3575510040451146E-5 4.3575510040451146E-5 0.0 2.178274383657092 1.7430204016180459E-4 52-53 4.3575510040451146E-5 4.3575510040451146E-5 0.0 2.505156072225536 1.7430204016180459E-4 54-55 4.3575510040451146E-5 4.3575510040451146E-5 0.0 2.867224985151645 1.7430204016180459E-4 56-57 4.3575510040451146E-5 4.3575510040451146E-5 0.0 3.2698409101703936 1.7430204016180459E-4 58-59 4.3575510040451146E-5 4.3575510040451146E-5 0.0 3.712480941161296 1.7430204016180459E-4 60-61 4.3575510040451146E-5 4.3575510040451146E-5 0.0 4.175143919015786 1.7430204016180459E-4 62-63 4.3575510040451146E-5 4.3575510040451146E-5 0.0 4.6204202683641356 2.1787755020225572E-4 64-65 4.3575510040451146E-5 4.3575510040451146E-5 0.0 5.071579311567946 2.1787755020225572E-4 66-67 4.3575510040451146E-5 4.3575510040451146E-5 0.0 5.574854664780138 2.1787755020225572E-4 68-69 4.3575510040451146E-5 4.3575510040451146E-5 0.0 6.12277312802877 2.1787755020225572E-4 70-71 8.715102008090229E-5 4.3575510040451146E-5 0.0 6.690431297325727 2.1787755020225572E-4 72-73 8.715102008090229E-5 4.3575510040451146E-5 0.0 7.260507907429929 2.1787755020225572E-4 74-75 8.715102008090229E-5 4.3575510040451146E-5 0.0 7.814221913513942 2.1787755020225572E-4 76-77 1.0893877510112786E-4 4.3575510040451146E-5 0.0 8.390704123594091 2.1787755020225572E-4 78-79 1.3072653012135343E-4 4.3575510040451146E-5 0.0 9.020849574289056 2.1787755020225572E-4 80-81 1.3072653012135343E-4 4.3575510040451146E-5 0.0 9.659644763727048 2.1787755020225572E-4 82-83 1.3072653012135343E-4 4.3575510040451146E-5 0.0 10.266956647160816 2.1787755020225572E-4 84-85 1.3072653012135343E-4 4.3575510040451146E-5 0.0 10.836227110329268 2.6145306024270685E-4 86-87 1.3072653012135343E-4 4.3575510040451146E-5 0.0 11.424060740774955 2.8324081526293245E-4 88-89 1.3072653012135343E-4 4.3575510040451146E-5 0.0 12.04154750580317 3.0502857028315804E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3990 0.0 46.492115 1 GTATCAA 7380 0.0 39.02184 1 ATCAACG 8815 0.0 33.908817 3 TATCAAC 9130 0.0 33.101223 2 TCAACGC 9010 0.0 32.965023 4 CAACGCA 9200 0.0 32.18108 5 AACGCAG 9465 0.0 31.380335 6 ACGCAGA 10710 0.0 27.20087 7 CGCAGAG 10785 0.0 26.746584 8 TACATGG 8300 0.0 26.694035 2 GTACATG 8480 0.0 25.903625 1 ACATGGG 8565 0.0 24.48439 3 GCAGAGT 11685 0.0 23.590754 9 GAGTACT 6230 0.0 22.466377 12-13 GTATACG 130 2.9665796E-4 21.896935 1 CATGGGA 5565 0.0 21.48519 4 CAGAGTA 11205 0.0 19.899298 10-11 AGAGTAC 10485 0.0 19.59166 10-11 GTACTTT 7250 0.0 19.242039 14-15 GAGTACA 6840 0.0 18.866364 1 >>END_MODULE