FastQCFastQC Report
Fri 27 May 2016
SRR939803_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939803_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2294867
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62190.2709960969415657No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50440.21979487264403558No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35020.15260143616165991No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40500.047.5248031
GTATCAA74800.039.102571
ATCAACG90400.031.9296423
TCAACGC91950.031.3900344
CAACGCA93050.031.0189555
TATCAAC94600.030.7128122
AACGCAG96700.029.9280556
ACGCAGA111950.025.7670027
CGCAGAG111650.025.7518048
TACATGG82100.024.3586222
GTACATG85550.024.097761
ACATGGG83600.023.017863
GCAGAGT123150.022.9260379
GAGTACT69900.020.09442912-13
AGAGTAC112400.019.87689610-11
CAGAGTA119150.019.34422710-11
AGTACTT75500.019.29070512-13
CATGGGG36450.019.149454
GTACTTT73900.018.8154314-15
CATGGGA49200.018.6922514
Produced by FastQC (version 0.11.5)