##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939803_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2294867 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.46733863008183 31.0 31.0 34.0 30.0 34.0 2 31.680561444301564 31.0 31.0 34.0 30.0 34.0 3 31.744289320470426 31.0 31.0 34.0 30.0 34.0 4 35.54675586864075 37.0 35.0 37.0 33.0 37.0 5 35.28204684628783 37.0 35.0 37.0 33.0 37.0 6 35.30401979722572 37.0 35.0 37.0 32.0 37.0 7 35.224245675239565 37.0 35.0 37.0 32.0 37.0 8 35.163700554324066 37.0 35.0 37.0 32.0 37.0 9 36.77940813127732 39.0 35.0 39.0 32.0 39.0 10-11 36.63667720177248 39.0 35.0 39.0 32.0 39.0 12-13 36.58570431314756 39.0 35.0 39.0 32.0 39.0 14-15 37.64902541193019 39.0 36.0 41.0 32.0 41.0 16-17 37.57944425537515 39.0 36.0 41.0 32.0 41.0 18-19 37.554534968693176 39.0 36.0 41.0 32.0 41.0 20-21 37.485659081768134 39.0 36.0 41.0 32.0 41.0 22-23 37.34635231584227 39.0 36.0 40.5 31.5 41.0 24-25 37.19635538791573 39.0 36.0 40.5 31.0 41.0 26-27 36.92407708159122 39.0 36.0 40.0 31.0 41.0 28-29 36.700843883327444 39.0 35.5 40.0 30.0 41.0 30-31 36.46095765026906 38.0 35.0 40.0 30.0 41.0 32-33 36.43379790637104 38.0 35.0 40.0 30.0 41.0 34-35 36.525669461454626 38.0 35.0 40.0 30.0 41.0 36-37 36.50593280569201 39.0 35.0 40.0 30.0 41.0 38-39 36.382678821909934 38.0 35.0 40.0 30.0 41.0 40-41 36.223375472304056 38.0 35.0 40.0 30.0 41.0 42-43 36.04595364350091 38.0 35.0 40.0 29.5 41.0 44-45 35.815705223875725 38.0 35.0 40.0 28.5 41.0 46-47 35.54648461109075 38.0 34.5 40.0 27.5 41.0 48-49 35.27066492306526 38.0 34.0 40.0 27.0 41.0 50-51 35.02384822301249 38.0 34.0 40.0 26.0 41.0 52-53 34.759715922534944 38.0 33.5 40.0 26.0 41.0 54-55 34.48395484357046 38.0 33.0 40.0 25.5 41.0 56-57 33.15261843061057 37.5 32.0 40.0 16.5 41.0 58-59 29.682795560701337 34.5 24.5 39.0 8.0 40.0 60-61 29.697629535829307 34.5 25.0 39.0 9.5 40.0 62-63 31.71475928670376 35.5 30.0 39.0 14.0 40.0 64-65 31.307693648477233 35.0 30.0 39.0 7.5 40.0 66-67 25.712944148833024 29.5 18.0 36.0 2.0 39.0 68-69 17.82978708570039 13.5 7.5 29.5 2.0 35.5 70-71 16.70249190911717 15.0 7.5 26.0 2.0 33.5 72-73 18.869680683019972 19.5 7.5 30.0 2.0 35.0 74-75 19.600265941337774 19.5 9.0 31.0 2.0 35.5 76-77 20.573735863559847 23.5 9.5 31.5 2.0 34.5 78-79 23.73410528801887 29.0 12.0 34.0 2.0 36.0 80-81 26.484774063159215 31.5 23.0 35.0 2.0 36.0 82-83 27.550438652871822 33.0 26.0 35.0 2.0 36.0 84-85 27.552004756702676 33.0 26.5 35.0 2.0 36.0 86-87 27.256461703445122 33.0 26.0 35.0 2.0 36.0 88-89 26.812847977682367 33.0 25.5 35.0 2.0 35.5 90-91 25.114364361856264 32.5 15.5 34.5 2.0 35.0 92-93 22.9211457570308 31.0 2.0 34.0 2.0 35.0 94-95 18.35994438893409 24.0 2.0 33.0 2.0 35.0 96-97 17.01822720009482 15.5 2.0 32.5 2.0 35.0 98-99 15.907018576675686 4.5 2.0 32.0 2.0 34.5 100-101 15.289480392545624 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.48977290132120643 1101 2 0.5227487287553636 1101 3 0.5069497374778997 1101 4 0.2844573684340901 1101 5 0.5055937123149192 1101 6 0.4923346490698606 1101 7 0.565755654448111 1101 8 0.4849093682227945 1101 9 0.6487061934516731 1101 10-11 0.6092775415627969 1101 12-13 0.6892825081951273 1101 14-15 0.7518281127896813 1101 16-17 0.6782173241659137 1101 18-19 0.7809055535799274 1101 20-21 0.8674736136814758 1101 22-23 0.6979224896314378 1101 24-25 0.6094668253939304 1101 26-27 0.71648667534825 1101 28-29 0.7095936728645427 1101 30-31 0.7131704097383391 1101 32-33 0.5784545750352947 1101 34-35 0.8637088407333309 1101 36-37 0.7662746665700908 1101 38-39 0.609683445759039 1101 40-41 0.5182938287161178 1101 42-43 0.5730368919795339 1101 44-45 0.3017037813009793 1101 46-47 0.3223106263553106 1101 48-49 0.4188617748803338 1101 50-51 0.45346443058959096 1101 52-53 0.48264841077154585 1101 54-55 0.4765812432936585 1101 56-57 1.208818910423389 1101 58-59 -8.132807259396728 1101 60-61 -9.663547100410145 1101 62-63 -2.4680297436146716 1101 64-65 0.05169501069923754 1101 66-67 -1.0403946031100801 1101 68-69 -3.523483783161822 1101 70-71 -4.472134799932315 1101 72-73 -7.019030113784385 1101 74-75 -7.629358735746926 1101 76-77 -8.73621582536493 1101 78-79 -11.857360376835206 1101 80-81 -7.436642893144576 1101 82-83 -3.55734129242283 1101 84-85 -2.6164340627628597 1101 86-87 -2.573318462060495 1101 88-89 -2.590739343767961 1101 90-91 -8.58126834873866 1101 92-93 -14.339012938468873 1101 94-95 -12.767166044274123 1101 96-97 -12.71803866056046 1101 98-99 -12.304291341214718 1101 100-101 -12.107313734090143 1102 1 0.4074267632348594 1102 2 0.4183511179816968 1102 3 0.4644167277084712 1102 4 0.4054601095160635 1102 5 0.45872848443300995 1102 6 0.4513536641152882 1102 7 0.4060517161577266 1102 8 0.49046025113337777 1102 9 0.5592947968523134 1102 10-11 0.5665027361233257 1102 12-13 0.48637025023295877 1102 14-15 0.6885163930284932 1102 16-17 0.7244585985954117 1102 18-19 0.7381596247208577 1102 20-21 0.6650012666624789 1102 22-23 0.6891975587981563 1102 24-25 0.8514896700587613 1102 26-27 0.7607844462590094 1102 28-29 0.819050533629337 1102 30-31 0.7085373634528338 1102 32-33 0.6023621904474936 1102 34-35 0.5485109137062807 1102 36-37 0.697775031548133 1102 38-39 0.48772430502371833 1102 40-41 0.645272742598479 1102 42-43 0.8369543985343384 1102 44-45 0.8414832811951598 1102 46-47 0.7182971837588426 1102 48-49 0.7217241019452558 1102 50-51 0.6859892562243957 1102 52-53 0.8541501866812453 1102 54-55 0.6284567040852949 1102 56-57 1.2871278101489096 1102 58-59 -8.823760226757784 1102 60-61 -10.446639387402417 1102 62-63 -3.072823950460908 1102 64-65 -0.17940554157449995 1102 66-67 -1.5984750927399176 1102 68-69 -4.0684120869990075 1102 70-71 -4.235982490957291 1102 72-73 -6.896824791063011 1102 74-75 -7.771508637365514 1102 76-77 -8.8191025768995 1102 78-79 -11.937998958651516 1102 80-81 -8.214431027501824 1102 82-83 -4.005280655990308 1102 84-85 -3.0069867056827455 1102 86-87 -2.745010750295556 1102 88-89 -2.8802594113464686 1102 90-91 -8.380919526957971 1102 92-93 -14.684610411366009 1102 94-95 -12.840274783606695 1102 96-97 -12.809579979108985 1102 98-99 -12.270815708479494 1102 100-101 -12.039656820031597 1103 1 0.2583314781414465 1103 2 0.29172912431902986 1103 3 0.19564396554316232 1103 4 0.20494469617534605 1103 5 0.18550342830317135 1103 6 0.20574275789974195 1103 7 0.26315524324722617 1103 8 0.24814614335254248 1103 9 0.22349809942245003 1103 10-11 0.34377433528989343 1103 12-13 0.3495005926682708 1103 14-15 0.4417219985093013 1103 16-17 0.3841755541359575 1103 18-19 0.3668666471554829 1103 20-21 0.40536973236988416 1103 22-23 0.3650651904940574 1103 24-25 0.36328732559180565 1103 26-27 0.1715348257486724 1103 28-29 0.36862400456014655 1103 30-31 0.3961010686571811 1103 32-33 0.4463157513014764 1103 34-35 0.40263537477323297 1103 36-37 0.16922932829462667 1103 38-39 0.15604167959012472 1103 40-41 0.36172008443930537 1103 42-43 0.39468848449812555 1103 44-45 0.2570175391557825 1103 46-47 0.1462564816885319 1103 48-49 0.2157221049349971 1103 50-51 0.33447245190254193 1103 52-53 0.2840580125373293 1103 54-55 0.19164187772706498 1103 56-57 0.8688254420179646 1103 58-59 -6.942231417287566 1103 60-61 -8.89951398554739 1103 62-63 -2.5720617966911163 1103 64-65 -0.238008557868028 1103 66-67 -1.7994308872440996 1103 68-69 -4.288746961377829 1103 70-71 -4.929726250300238 1103 72-73 -7.825119136522698 1103 74-75 -8.573325858981708 1103 76-77 -9.221734186224268 1103 78-79 -12.520948184954333 1103 80-81 -8.863924848890608 1103 82-83 -4.779327025875855 1103 84-85 -3.8271230333771236 1103 86-87 -3.590416760950127 1103 88-89 -3.6080824970159036 1103 90-91 -9.12284548751413 1103 92-93 -14.88006288425342 1103 94-95 -13.012269600614758 1103 96-97 -12.952515988205192 1103 98-99 -12.500420808358864 1103 100-101 -12.11699883401067 1104 1 0.18261830542180135 1104 2 0.22146662881381474 1104 3 0.19756373860640863 1104 4 0.191988725101794 1104 5 0.17967319137800075 1104 6 0.31647131277608764 1104 7 0.2626248584935382 1104 8 0.2754484726505524 1104 9 0.3174461197189089 1104 10-11 0.32485178544770577 1104 12-13 0.21790424443222633 1104 14-15 0.3486090095764638 1104 16-17 0.4251869808809303 1104 18-19 0.3972054541393746 1104 20-21 0.37953220390290454 1104 22-23 0.5388675797225488 1104 24-25 0.41957253474917877 1104 26-27 0.2871277379704935 1104 28-29 0.2779946346292661 1104 30-31 0.384283277295161 1104 32-33 0.26991337805444005 1104 34-35 0.2272488231730918 1104 36-37 0.3611986438885353 1104 38-39 0.43490070312282114 1104 40-41 0.4627473927616492 1104 42-43 0.4842416028808003 1104 44-45 0.3179623431831118 1104 46-47 0.43155353710425004 1104 48-49 0.37748545944376843 1104 50-51 0.17577953568535065 1104 52-53 0.35210058315541914 1104 54-55 0.31930528817095194 1104 56-57 1.0996561783638015 1104 58-59 -6.845835236856868 1104 60-61 -9.109581307453976 1104 62-63 -2.591283998936511 1104 64-65 -0.39734123196607385 1104 66-67 -1.8737386901926811 1104 68-69 -4.5578647847027725 1104 70-71 -4.890263027638527 1104 72-73 -7.722444239403359 1104 74-75 -8.747074810198296 1104 76-77 -9.363347914299702 1104 78-79 -12.495629554565584 1104 80-81 -8.360522795272807 1104 82-83 -4.493136506877892 1104 84-85 -3.639415773798234 1104 86-87 -3.3690405573463025 1104 88-89 -3.5018322473055647 1104 90-91 -9.271966007710702 1104 92-93 -15.360524548722635 1104 94-95 -13.212721076902262 1104 96-97 -12.952552945334922 1104 98-99 -12.177819026425908 1104 100-101 -11.715494079525872 1105 1 0.14782440677334563 1105 2 0.15483699449463018 1105 3 0.1850411550011195 1105 4 0.08353755538335861 1105 5 0.1584771078210494 1105 6 0.20494931733161081 1105 7 0.22482650643300417 1105 8 0.2141092223105261 1105 9 0.24072976571689253 1105 10-11 0.21203221126525307 1105 12-13 0.18873599504114935 1105 14-15 0.2706659590640683 1105 16-17 0.31245496709727405 1105 18-19 0.18464046032732284 1105 20-21 0.2787057664828083 1105 22-23 0.3444269928143058 1105 24-25 0.37059682973906405 1105 26-27 0.41832929469056523 1105 28-29 0.40547415111205964 1105 30-31 0.36101101670180213 1105 32-33 0.27862772250701084 1105 34-35 0.29600781747255667 1105 36-37 0.15520553027138817 1105 38-39 0.2083034959336416 1105 40-41 0.24345182085014727 1105 42-43 0.23151370077689393 1105 44-45 0.34524655487744127 1105 46-47 0.21631799367496996 1105 48-49 0.2141035415766126 1105 50-51 0.31719187725974507 1105 52-53 0.47795823258161363 1105 54-55 0.23013127630154173 1105 56-57 0.8046236695447959 1105 58-59 -6.468429325568401 1105 60-61 -8.137527285155603 1105 62-63 -2.362299430669694 1105 64-65 0.02636660119231138 1105 66-67 -1.3753918713666309 1105 68-69 -3.843440344987213 1105 70-71 -4.7462097487100685 1105 72-73 -6.167262637944937 1105 74-75 -6.600792527725897 1105 76-77 -8.460198394835256 1105 78-79 -11.82002611110186 1105 80-81 -7.726599122753125 1105 82-83 -3.5450785437659214 1105 84-85 -2.650636609122735 1105 86-87 -2.381458136547195 1105 88-89 -2.3656198361593717 1105 90-91 -8.027305149812808 1105 92-93 -11.052086789615334 1105 94-95 -11.948685544909447 1105 96-97 -12.277832594017756 1105 98-99 -11.79492463532892 1105 100-101 -11.368767238698254 1106 1 0.10338688622302072 1106 2 0.10715700954213503 1106 3 0.10538181298483806 1106 4 -0.014518006952506823 1106 5 0.06980236508599091 1106 6 0.056262779248356765 1106 7 0.0693143887408425 1106 8 0.12905528533487143 1106 9 0.10940134244552979 1106 10-11 0.07673775471994304 1106 12-13 0.15689465719165696 1106 14-15 0.12776007488677976 1106 16-17 0.025694922385518737 1106 18-19 0.18081118991590728 1106 20-21 0.054076174876094285 1106 22-23 0.10384101599721873 1106 24-25 0.08935809203514822 1106 26-27 0.23819815669433098 1106 28-29 0.26720070507560223 1106 30-31 0.3217508319965532 1106 32-33 0.2886460508843314 1106 34-35 0.27615010110366 1106 36-37 0.3404350334309285 1106 38-39 0.1658214868907848 1106 40-41 0.24513470402666115 1106 42-43 0.13878408275090948 1106 44-45 0.4199220392189815 1106 46-47 0.22983490273377072 1106 48-49 0.20452233387079843 1106 50-51 0.11404993894589666 1106 52-53 0.20778113364858086 1106 54-55 0.006790791335404833 1106 56-57 0.8133264181552704 1106 58-59 -6.218347303746647 1106 60-61 -8.668664886543187 1106 62-63 -2.459489815343062 1106 64-65 -0.2529113303788755 1106 66-67 -1.6581355389948271 1106 68-69 -4.088577366299248 1106 70-71 -4.39771509143565 1106 72-73 -6.014395320176963 1106 74-75 -6.506847748334085 1106 76-77 -8.473878001446392 1106 78-79 -12.129894413131694 1106 80-81 -7.901927138248606 1106 82-83 -3.8968084862896255 1106 84-85 -3.026751890523993 1106 86-87 -2.7853807000503963 1106 88-89 -3.0712707089289886 1106 90-91 -8.661872896606955 1106 92-93 -11.937958360313477 1106 94-95 -12.220456864480823 1106 96-97 -12.682842081268298 1106 98-99 -12.246038373212304 1106 100-101 -12.024104095320519 1107 1 0.019769138782745443 1107 2 0.031218442989235484 1107 3 -0.010659142646559872 1107 4 -0.027316641764791427 1107 5 -0.1989689659720284 1107 6 -0.11822527535915128 1107 7 -0.0684842460714421 1107 8 2.1569489118178353E-4 1107 9 0.006159021626416461 1107 10-11 -0.0981769210479797 1107 12-13 0.06389124422236137 1107 14-15 0.021958027105206668 1107 16-17 -0.09695866123223595 1107 18-19 0.06711665066676886 1107 20-21 0.005441362589408527 1107 22-23 0.06821814910303203 1107 24-25 0.031550924799653046 1107 26-27 0.14327494850320477 1107 28-29 0.10252493715751143 1107 30-31 0.09265526885662467 1107 32-33 0.08877403723246147 1107 34-35 0.054733718851103674 1107 36-37 -0.04651035906054091 1107 38-39 0.09713548006484274 1107 40-41 0.09987019890720461 1107 42-43 0.08950933872360878 1107 44-45 0.0959032678879268 1107 46-47 0.13683148976448933 1107 48-49 0.12837045673769865 1107 50-51 0.23435710618139183 1107 52-53 0.2384978571980696 1107 54-55 0.06246485276885494 1107 56-57 0.797968056380526 1107 58-59 -6.625557201357566 1107 60-61 -9.42677068859097 1107 62-63 -2.9823140474249747 1107 64-65 -0.7298737877167589 1107 66-67 -1.901949532168885 1107 68-69 -4.25261320247672 1107 70-71 -4.720667309865682 1107 72-73 -6.263542077856144 1107 74-75 -6.928562765398931 1107 76-77 -9.323707352982229 1107 78-79 -12.637573594019068 1107 80-81 -8.62611655804383 1107 82-83 -4.331108827586551 1107 84-85 -3.3808474536639217 1107 86-87 -3.1667800174565386 1107 88-89 -3.1301444290655063 1107 90-91 -8.59340020036122 1107 92-93 -12.015816722088221 1107 94-95 -12.218323758678775 1107 96-97 -12.865222627343382 1107 98-99 -12.159647588229369 1107 100-101 -11.754479521941679 1108 1 0.02264930794851594 1108 2 -0.01673841752244698 1108 3 0.08860848236746932 1108 4 0.04572252909014196 1108 5 -0.12429692557621763 1108 6 -0.005180887358726238 1108 7 -0.014451108536952972 1108 8 0.03224813680627392 1108 9 0.1392116475896472 1108 10-11 0.03207426060430407 1108 12-13 -0.0025778221926557876 1108 14-15 -4.921536588398112E-4 1108 16-17 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60-61 3.994398240077441 2311 62-63 1.490189187175396 2311 64-65 1.2637778098829777 2311 66-67 -0.538838352431597 2311 68-69 -3.478097127977085 2311 70-71 -0.8823011557655924 2311 72-73 -1.061822814546364 2311 74-75 1.310932437276069 2311 76-77 4.462917175138827 2311 78-79 5.238623407785919 2311 80-81 3.0324324374996117 2311 82-83 1.5741017409044495 2311 84-85 1.1076111461135163 2311 86-87 1.07291085857441 2311 88-89 1.1304508838255067 2311 90-91 2.7385237079944282 2311 92-93 4.768078362384099 2311 94-95 8.729752253098898 2311 96-97 9.625679892716466 2311 98-99 10.303811743659265 2311 100-101 9.596893585883654 2312 1 -0.21896098006983422 2312 2 -0.26194412606526285 2312 3 -0.23890814508134284 2312 4 -0.168021779392717 2312 5 -0.28366846698897064 2312 6 -0.20059088802322123 2312 7 -0.19570087206934517 2312 8 -0.2556487591807226 2312 9 -0.26837553702614514 2312 10-11 -0.2485578733653 2312 12-13 -0.3652602440407122 2312 14-15 -0.3403074815929159 2312 16-17 -0.3268179330430314 2312 18-19 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1.7459033075638253 2312 84-85 1.3501015896623478 2312 86-87 1.1230579466818966 2312 88-89 1.240797658698849 2312 90-91 2.814464662625568 2312 92-93 4.985554512487269 2312 94-95 8.915854442683742 2312 96-97 9.802636262278998 2312 98-99 10.300530817590586 2312 100-101 9.46555560331189 2313 1 -0.21098913988176804 2313 2 -0.28888742036937387 2313 3 -0.24981819655240045 2313 4 -0.26259676411395105 2313 5 -0.32485609955469386 2313 6 -0.31073786110915336 2313 7 -0.3839727230424188 2313 8 -0.28688995728568756 2313 9 -0.26327157111302313 2313 10-11 -0.37778008673026875 2313 12-13 -0.32873306347622844 2313 14-15 -0.4983373888887428 2313 16-17 -0.45746955071606266 2313 18-19 -0.5028100488352578 2313 20-21 -0.5465338678371054 2313 22-23 -0.3828821247519869 2313 24-25 -0.48617301299029236 2313 26-27 -0.42262993115727454 2313 28-29 -0.6150912809182785 2313 30-31 -0.6064321365373715 2313 32-33 -0.4724799905481589 2313 34-35 -0.42731583392048833 2313 36-37 -0.6751190120616997 2313 38-39 -0.7930034400471513 2313 40-41 -0.8934128595492652 2313 42-43 -0.6528768786028323 2313 44-45 -0.6246080984578768 2313 46-47 -0.4895188811660063 2313 48-49 -0.5600128449183757 2313 50-51 -0.47917802492158756 2313 52-53 -0.5712733001459398 2313 54-55 -0.593043073358416 2313 56-57 0.6728556532005285 2313 58-59 4.2283213044294925 2313 60-61 3.9734783014834854 2313 62-63 1.309462808419653 2313 64-65 0.9959161852383538 2313 66-67 1.8834256219597165 2313 68-69 0.5711480633874544 2313 70-71 3.12805325242271 2313 72-73 8.614481908301233 2313 74-75 10.082913514123934 2313 76-77 8.839394174338558 2313 78-79 6.440947776209761 2313 80-81 3.593125248445581 2313 82-83 2.0606522747820897 2313 84-85 1.6950129415865298 2313 86-87 1.5135481487366746 2313 88-89 1.595788772790165 2313 90-91 2.8328246536849555 2313 92-93 4.744130703878955 2313 94-95 8.812127284627106 2313 96-97 9.561462228201503 2313 98-99 9.927588604913355 2313 100-101 9.236821919543948 2314 1 -0.41113387034116755 2314 2 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3.2725983880036758 2314 62-63 0.8200233606220166 2314 64-65 0.7167378884454827 2314 66-67 1.5209911283970428 2314 68-69 -0.2210818194763 2314 70-71 2.4728183039928773 2314 72-73 7.974899718099962 2314 74-75 9.569743837540845 2314 76-77 8.571905247809145 2314 78-79 6.190719547408399 2314 80-81 2.9656785482205343 2314 82-83 1.394387990601448 2314 84-85 1.1086195928258853 2314 86-87 1.0374561097071648 2314 88-89 1.0343387644403315 2314 90-91 2.3563513055604837 2314 92-93 4.417299108106359 2314 94-95 8.400448252253604 2314 96-97 9.12245736939322 2314 98-99 9.110086954827073 2314 100-101 8.248582462203649 2315 1 -0.6730822211124305 2315 2 -0.46490370297599526 2315 3 -0.4664804295047702 2315 4 -0.38912392772470383 2315 5 -0.6072944081847069 2315 6 -0.6520359857316578 2315 7 -0.5573284424502063 2315 8 -0.7156667708003184 2315 9 -0.9836281755905674 2315 10-11 -0.8141092724493575 2315 12-13 -0.6797426955114076 2315 14-15 -0.666451537791275 2315 16-17 -0.8565613921798274 2315 18-19 -0.7813380724192669 2315 20-21 -0.9269028867146929 2315 22-23 -0.803698413381774 2315 24-25 -0.7685035739973571 2315 26-27 -0.7171891781285424 2315 28-29 -0.9366031607629495 2315 30-31 -0.9203241346032627 2315 32-33 -0.9083131021957769 2315 34-35 -0.9747900389053257 2315 36-37 -0.9379147115261404 2315 38-39 -0.8536970570043465 2315 40-41 -0.8312961039573423 2315 42-43 -0.8588591303608482 2315 44-45 -0.7224444458803845 2315 46-47 -0.7109681034255928 2315 48-49 -0.6540906574408609 2315 50-51 -0.6944291655573309 2315 52-53 -0.6669444055462037 2315 54-55 -0.7038765614534697 2315 56-57 0.736815328150719 2315 58-59 4.172217752940682 2315 60-61 3.87513977317548 2315 62-63 1.3876412378140088 2315 64-65 1.1971119496190603 2315 66-67 2.005056710160517 2315 68-69 6.333607720770093 2315 70-71 5.388835041735955 2315 72-73 9.177038856433466 2315 74-75 10.253923631358035 2315 76-77 8.905007541066958 2315 78-79 6.286770882720958 2315 80-81 3.2996679886148677 2315 82-83 1.7085549606090957 2315 84-85 1.1513272199869817 2315 86-87 1.0054535249080558 2315 88-89 1.1146490405195841 2315 90-91 2.3892642472555643 2315 92-93 4.432213632740432 2315 94-95 8.510988154672429 2315 96-97 9.430562083812994 2315 98-99 9.474534779621775 2315 100-101 8.656163370757197 2316 1 -0.4454464056078251 2316 2 -0.44979050690017175 2316 3 -0.4863973986632004 2316 4 -0.3340015939392771 2316 5 -0.5017923557975763 2316 6 -0.506684165924419 2316 7 -0.45154830149923697 2316 8 -0.499198387635289 2316 9 -0.6229350767198341 2316 10-11 -0.40959114272985175 2316 12-13 -0.50995559695005 2316 14-15 -0.7356892945195455 2316 16-17 -0.8119211625139329 2316 18-19 -0.7764580318392049 2316 20-21 -0.6328886261316811 2316 22-23 -0.7218543948041685 2316 24-25 -2.043924034552319 2316 26-27 -1.3413362952531145 2316 28-29 -0.9547197493033934 2316 30-31 -0.7014121336247356 2316 32-33 -0.4708754742588539 2316 34-35 -0.5594374276429193 2316 36-37 -0.5148378576323651 2316 38-39 -0.7576304092846371 2316 40-41 -0.7103211031478978 2316 42-43 -0.7140947861354121 2316 44-45 -0.7090927771691256 2316 46-47 -0.48042890660867954 2316 48-49 -0.6740684247458475 2316 50-51 -0.7487496113624417 2316 52-53 -0.6086573303687572 2316 54-55 -0.46314450140361174 2316 56-57 0.6475260548864341 2316 58-59 4.180023479478535 2316 60-61 4.087126541484171 2316 62-63 1.6237355504891582 2316 64-65 1.1642910235445676 2316 66-67 6.367275518732697 2316 68-69 9.245995792821294 2316 70-71 6.255473947513217 2316 72-73 9.802591525975757 2316 74-75 10.610577071130095 2316 76-77 9.018751339835887 2316 78-79 6.420368334235338 2316 80-81 3.4320491174457786 2316 82-83 1.8283850495758536 2316 84-85 1.5032342671756957 2316 86-87 1.3861600065660795 2316 88-89 1.5056099030618135 2316 90-91 2.964448530395053 2316 92-93 4.994841299854389 2316 94-95 9.000969771327522 2316 96-97 9.780593921895655 2316 98-99 10.206137944003414 2316 100-101 9.259069270335926 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 13.0 4 42.0 5 89.0 6 177.0 7 526.0 8 1123.0 9 2068.0 10 3538.0 11 5306.0 12 7431.0 13 10140.0 14 13537.0 15 18425.0 16 24255.0 17 31899.0 18 35056.0 19 36195.0 20 38115.0 21 38469.0 22 37022.0 23 35823.0 24 37423.0 25 40379.0 26 54132.0 27 78215.0 28 109695.0 29 146106.0 30 174886.0 31 187844.0 32 194032.0 33 201072.0 34 205605.0 35 201079.0 36 172567.0 37 111626.0 38 38810.0 39 2144.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.69627170473153 20.049486286667424 14.456106308782399 23.798135699818644 2 15.365270249129209 21.2171418884734 39.81679073956979 23.6007971228276 3 18.804087735268986 25.26378495071613 30.633131816868552 25.298995497146333 4 12.76531493982004 17.46236274259031 37.912480331104156 31.859841986485492 5 12.920936276348034 38.24469987005852 35.19957790820376 13.634785945389694 6 31.155283140714968 37.663244825623806 17.554271142628807 13.627200891032418 7 27.264063669049232 33.22628283033396 21.679949208385494 17.829704292231312 8 25.62318534919078 36.311753686385636 20.07161211802186 17.993448846401723 9 26.01244429415735 16.41524323631827 20.465063988457718 37.107248481066655 10-11 24.431808030705046 27.132857808317436 28.188866718637723 20.246467442339796 12-13 25.212659382874914 24.33106145149152 28.506619337852694 21.949659827780867 14-15 22.03451796468896 25.877187439947395 26.53805477712613 25.550239818237515 16-17 21.57103222103939 29.05355735212542 27.3404079626401 22.03500246419509 18-19 21.524051558492012 27.79198725134601 29.3990776225085 21.28488356765347 20-21 22.399337303643303 27.025836355658083 29.273613677829697 21.301212662868917 22-23 21.859741762812398 27.091243196228803 29.11094629884869 21.938068742110108 24-25 21.663477665590207 27.21183842026575 29.413752518119786 21.710931396024257 26-27 21.67681359836278 27.367781409316322 29.166689362269537 21.78871563005136 28-29 21.580230354482353 27.564666545523792 29.16913419013592 21.685968909857937 30-31 21.804376280551566 27.354204784967568 29.10036342987682 21.74105550460405 32-33 21.527110071104634 27.32755737556689 29.04823066355972 22.097101889768755 34-35 21.897295895801243 27.445165141872323 28.97574781739072 21.681791144935715 36-37 21.977954441736706 27.142585656569974 29.138750850496177 21.740709051197147 38-39 21.664990394579558 27.40784408163354 29.13405200547885 21.79311351830805 40-41 21.844999374515904 27.28915192638205 29.165015780941957 21.700832918160092 42-43 21.677777591318332 27.225822705689772 28.975659850314717 22.120739852677183 44-45 21.71044186378491 27.302145298856573 29.046433346664035 21.940979490694478 46-47 21.77517183841936 27.44837873191986 28.668781310466006 22.107668119194773 48-49 21.656455610073127 27.51942047722405 28.663432227040488 22.160691685662336 50-51 21.73615610546189 27.583071648731767 28.51868535932679 22.162086886479553 52-53 21.818028081475465 27.509507555376565 28.45922794575131 22.213236417396665 54-55 21.55596876940118 27.500320878734858 28.586993875084143 22.35671647677982 56-57 21.573678160618574 27.592772487132695 28.413055223162814 22.42049412908592 58-59 20.060601643528166 27.323578882019756 30.112558453606574 22.503261020845507 60-61 21.62036766896113 27.453685158073966 28.547067497034945 22.378879675929962 62-63 21.428929371664825 27.41749162693291 28.54989284740058 22.603686154001686 64-65 20.69167005408302 27.42452279976671 29.050591745089992 22.83321540106028 66-67 20.83885537542483 27.37706370784657 29.0192123971309 22.764868519597698 68-69 21.585749894536217 26.83162158071182 29.059482470620164 22.523146054131807 70-71 20.782446186668402 26.9648739765684 29.255360731451947 22.997319105311252 72-73 20.488529251490792 27.01844886579929 29.30975737793705 23.183264504772865 74-75 21.071103646703456 27.115259960488014 28.68434208694717 23.129294305861357 76-77 19.46234986144446 27.41214203914805 29.498957253335224 23.626550846072263 78-79 20.44873496141445 27.41201003917958 28.46371277671796 23.675542222688012 80-81 20.97030341073168 27.275765684130114 28.04156420033558 23.712366704802626 82-83 21.188105782349535 27.272390552290048 27.7843601121287 23.75514355323172 84-85 21.182805444849503 27.102802516573952 27.69767385110939 24.016718187467152 86-87 21.112307779796595 27.05340103142043 27.749889805043715 24.084401383739255 88-89 21.14894265091237 27.01928412829243 27.652480002649394 24.179293218145805 90-91 20.795252409541472 27.06495160390802 27.759852490510976 24.379943496039523 92-93 20.619506902677195 26.89932478104 27.956557428750834 24.524610887531967 94-95 19.375570799938732 27.042685793310945 28.660193223366015 24.92155018338431 96-97 19.126244485075887 26.77308368385677 29.010453696703774 25.090218134363568 98-99 19.375266007430383 26.748562844654302 28.55329809601399 25.322873051901322 100-101 19.31264339252495 26.648915598131673 28.668267354542447 25.370173654800926 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 182.0 1 148.0 2 119.5 3 140.0 4 163.0 5 190.5 6 254.0 7 404.0 8 575.5 9 737.0 10 848.0 11 950.0 12 1169.5 13 1350.5 14 1502.0 15 1726.0 16 1966.5 17 2264.0 18 2567.5 19 2917.5 20 3385.5 21 4095.0 22 5054.5 23 6298.5 24 8023.5 25 10369.0 26 13348.5 27 16760.5 28 20506.5 29 24646.0 30 29425.0 31 34691.5 32 39951.5 33 45375.5 34 50905.5 35 56745.0 36 62683.0 37 67727.5 38 71788.0 39 75214.5 40 78430.5 41 81569.0 42 84240.0 43 86361.0 44 88603.5 45 90537.5 46 91689.5 47 92415.0 48 92481.5 49 90959.5 50 88000.0 51 84503.5 52 80442.0 53 75939.5 54 70553.5 55 63803.0 56 56500.0 57 49027.0 58 41892.5 59 34854.0 60 27772.5 61 21727.0 62 16425.5 63 11809.5 64 8293.0 65 5797.5 66 4128.5 67 2909.5 68 2004.0 69 1379.0 70 940.5 71 640.5 72 414.0 73 276.0 74 189.5 75 115.5 76 71.5 77 39.0 78 20.5 79 13.0 80 8.5 81 4.0 82 2.0 83 1.5 84 1.0 85 1.0 86 0.5 87 0.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021526301959982865 2 0.0049676081446114306 3 0.004357551004045114 4 0.0 5 0.0665398038317689 6 4.3575510040451146E-5 7 0.0 8 1.7430204016180459E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 1.7430204016180459E-4 16-17 0.0 18-19 0.0015905061164764667 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.0893877510112787E-4 28-29 0.0024402285622652643 30-31 0.008780465273150906 32-33 4.1396734538428595E-4 34-35 9.586612208899251E-4 36-37 0.026929665204998808 38-39 0.02568776316884595 40-41 0.028062628466050538 42-43 0.028476595811434826 44-45 0.028955926421879788 46-47 0.030764310088558507 48-49 0.03065537131345738 50-51 0.026036367249169558 52-53 0.022114571345528956 54-55 0.016841934630634367 56-57 0.14820030964757433 58-59 0.7497602257560024 60-61 0.30356879069680287 62-63 3.921795903640603E-4 64-65 0.14369024435838765 66-67 1.4093845089933317 68-69 2.6961475327328337 70-71 2.1747447673438156 72-73 1.8427865318556589 74-75 1.5492183207131394 76-77 1.10594644482665 78-79 0.41921819434416024 80-81 3.268163253033836E-4 82-83 1.7430204016180459E-4 84-85 4.3575510040451146E-5 86-87 0.003072073457851806 88-89 2.1787755020225573E-5 90-91 0.22293230936694808 92-93 0.17345231771601577 94-95 1.2813814482495065 96-97 1.2623825258718697 98-99 0.995438951364066 100-101 0.4001539087014629 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2294867.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.14631728923066 #Duplication Level Percentage of deduplicated Percentage of total 1 70.29080587874398 37.356974717474465 2 15.097783021010228 16.04783133597138 3 6.108269842421105 9.738961414006528 4 3.013369986694846 6.405980696909161 5 1.6910023603543693 4.493527399011564 6 1.0485882494401617 3.343716228630346 7 0.6622539637878153 2.4637451499862535 8 0.4518076245969952 1.9209529096420417 9 0.3226541979155214 1.5433094139408472 >10 1.228420128184671 10.807725340036397 >50 0.0571213018911798 2.054142390049815 >100 0.02579103145609258 2.471630064771613 >500 0.0017223339832144857 0.6090175795547742 >1k 2.4604771188748174E-4 0.25154884423184015 >5k 1.6403180792498785E-4 0.4909365157829172 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6219 0.2709960969415657 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5044 0.21979487264403558 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3502 0.15260143616165991 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.3575510040451146E-5 7 0.0 0.0 0.0 0.0 4.3575510040451146E-5 8 0.0 0.0 0.0 0.0 4.3575510040451146E-5 9 0.0 0.0 0.0 4.3575510040451146E-5 4.3575510040451146E-5 10-11 0.0 0.0 0.0 4.3575510040451146E-5 4.3575510040451146E-5 12-13 0.0 0.0 0.0 4.3575510040451146E-5 1.7430204016180459E-4 14-15 0.0 0.0 0.0 6.536326506067673E-5 1.9608979518203015E-4 16-17 0.0 0.0 0.0 1.3072653012135343E-4 2.3966530522248129E-4 18-19 0.0 0.0 0.0 2.1787755020225572E-4 2.6145306024270685E-4 20-21 0.0 0.0 0.0 3.9217959036406025E-4 2.6145306024270685E-4 22-23 0.0 0.0 0.0 7.62571425707895E-4 2.6145306024270685E-4 24-25 0.0 0.0 0.0 0.0013944163212944367 2.6145306024270685E-4 26-27 0.0 0.0 0.0 0.002701681622507971 2.8324081526293245E-4 28-29 0.0 0.0 0.0 0.008671526498049777 3.0502857028315804E-4 30-31 0.0 0.0 0.0 0.030350342743174226 3.0502857028315804E-4 32-33 0.0 0.0 0.0 0.08292419560697853 3.0502857028315804E-4 34-35 0.0 0.0 0.0 0.16316849734646932 3.0502857028315804E-4 36-37 0.0 0.0 0.0 0.2984704560220701 3.0502857028315804E-4 38-39 0.0 0.0 0.0 0.5062384878949412 3.0502857028315804E-4 40-41 0.0 0.0 0.0 0.7338987401012782 3.0502857028315804E-4 42-43 0.0 0.0 0.0 0.9588790984401275 3.0502857028315804E-4 44-45 0.0 0.0 0.0 1.234232746385738 3.268163253033836E-4 46-47 0.0 0.0 0.0 1.5485864758175527 3.4860408032360917E-4 48-49 0.0 0.0 0.0 1.854116164466176 3.4860408032360917E-4 50-51 0.0 0.0 0.0 2.1524341062031045 3.4860408032360917E-4 52-53 0.0 0.0 0.0 2.43116485617685 3.4860408032360917E-4 54-55 0.0 0.0 0.0 2.744363834592593 3.4860408032360917E-4 56-57 0.0 0.0 0.0 3.0115034988955784 3.4860408032360917E-4 58-59 0.0 0.0 0.0 3.199313947169923 3.4860408032360917E-4 60-61 0.0 2.1787755020225573E-5 0.0 3.3401064201106205 3.4860408032360917E-4 62-63 2.1787755020225572E-4 4.3575510040451146E-5 0.0 3.4581960523202433 3.4860408032360917E-4 64-65 2.1787755020225572E-4 4.3575510040451146E-5 0.0 3.5804907212487693 3.4860408032360917E-4 66-67 2.1787755020225572E-4 4.3575510040451146E-5 0.0 3.680670818831767 3.4860408032360917E-4 68-69 2.1787755020225572E-4 4.3575510040451146E-5 0.0 3.7960151939088407 3.4860408032360917E-4 70-71 2.1787755020225572E-4 4.3575510040451146E-5 0.0 4.002737413540741 3.921795903640603E-4 72-73 2.1787755020225572E-4 4.3575510040451146E-5 0.0 4.267001094181057 3.921795903640603E-4 74-75 2.1787755020225572E-4 4.3575510040451146E-5 0.0 4.5638592563316305 3.921795903640603E-4 76-77 2.1787755020225572E-4 4.3575510040451146E-5 0.0 4.98695567106939 3.921795903640603E-4 78-79 2.1787755020225572E-4 4.3575510040451146E-5 0.0 5.593657497362592 3.921795903640603E-4 80-81 2.1787755020225572E-4 4.3575510040451146E-5 0.0 6.261517551997567 3.921795903640603E-4 82-83 2.1787755020225572E-4 4.3575510040451146E-5 0.0 6.893013843503785 3.921795903640603E-4 84-85 2.1787755020225572E-4 8.715102008090229E-5 0.0 7.397487523242088 3.921795903640603E-4 86-87 2.1787755020225572E-4 8.715102008090229E-5 0.0 7.855466133767229 3.921795903640603E-4 88-89 2.1787755020225572E-4 8.715102008090229E-5 0.0 8.318368776926942 3.921795903640603E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4050 0.0 47.524803 1 GTATCAA 7480 0.0 39.10257 1 ATCAACG 9040 0.0 31.929642 3 TCAACGC 9195 0.0 31.390034 4 CAACGCA 9305 0.0 31.018955 5 TATCAAC 9460 0.0 30.712812 2 AACGCAG 9670 0.0 29.928055 6 ACGCAGA 11195 0.0 25.767002 7 CGCAGAG 11165 0.0 25.751804 8 TACATGG 8210 0.0 24.358622 2 GTACATG 8555 0.0 24.09776 1 ACATGGG 8360 0.0 23.01786 3 GCAGAGT 12315 0.0 22.926037 9 GAGTACT 6990 0.0 20.094429 12-13 AGAGTAC 11240 0.0 19.876896 10-11 CAGAGTA 11915 0.0 19.344227 10-11 AGTACTT 7550 0.0 19.290705 12-13 CATGGGG 3645 0.0 19.14945 4 GTACTTT 7390 0.0 18.81543 14-15 CATGGGA 4920 0.0 18.692251 4 >>END_MODULE