FastQCFastQC Report
Fri 27 May 2016
SRR939802_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939802_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2358562
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58990.25011002466757287No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42860.1817208960375008No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29560.12533060398666646No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24240.10277448716633272No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44450.041.8130531
GTATCAA80050.039.1470871
ATCAACG96000.032.2515373
TATCGCG350.003616675532.2455594-95
TCAACGC97650.031.7547664
TATCAAC97950.031.6094722
CAACGCA99150.031.1787875
AACGCAG102700.030.055866
ACGCAGA114850.026.6304267
CGCAGAG115250.025.9669658
GTACATG86900.024.4198671
TACATGG84450.024.1816462
GCAGAGT123700.023.0900029
ACATGGG87000.022.4993443
GAGTACT65400.021.04453312-13
GTGTAGC15750.020.9124071
AGAGTAC109900.019.8232310-11
TAGCGCG1908.958636E-619.8121744
CATGGGG38750.019.6715724
GTACTTT72150.018.98151214-15
Produced by FastQC (version 0.11.5)