FastQCFastQC Report
Fri 27 May 2016
SRR939802_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939802_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2358562
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62440.26473758162812766No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52660.22327163754864193No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35410.15013385274586802No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42200.042.5127561
GTATCAA75150.039.093231
ATCAACG93600.030.9646243
TCAACGC94700.030.5548634
TATCAAC96450.030.204882
CAACGCA96450.030.0004715
AACGCAG99700.029.0701036
ACGCAGA113450.025.3377847
GTACATG89350.025.018791
CGCAGAG114100.024.9855738
TACATGG89150.024.5884742
ACATGGG89150.023.358433
GCAGAGT125800.022.13399
GTACCGT2457.64885E-921.2974246
GAGTACT66450.021.02281212-13
AGAGTAC112700.020.22413410-11
CATGGGA57150.020.0032834
AGTACTT72850.019.7619412-13
GTACTTT75650.018.37211614-15
CAGAGTA120950.018.27597410-11
Produced by FastQC (version 0.11.5)