##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939802_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2358562 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.4321344955104 31.0 31.0 34.0 30.0 34.0 2 31.66192027175881 31.0 31.0 34.0 30.0 34.0 3 31.71082846242753 31.0 31.0 34.0 30.0 34.0 4 35.51950468124222 37.0 35.0 37.0 33.0 37.0 5 35.25641004985241 37.0 35.0 37.0 33.0 37.0 6 35.27493150487458 37.0 35.0 37.0 32.0 37.0 7 35.18133845962073 37.0 35.0 37.0 32.0 37.0 8 35.13893041607556 37.0 35.0 37.0 32.0 37.0 9 36.73039334984622 39.0 35.0 39.0 32.0 39.0 10-11 36.59952759350825 39.0 35.0 39.0 32.0 39.0 12-13 36.52315012282908 38.0 35.0 39.0 32.0 39.0 14-15 37.575735342127956 39.0 36.0 41.0 32.0 41.0 16-17 37.497525186957134 39.0 36.0 41.0 32.0 41.0 18-19 37.47696944154956 39.0 36.0 41.0 32.0 41.0 20-21 37.396127810080884 39.0 36.0 41.0 32.0 41.0 22-23 37.271150599390644 39.0 36.0 40.0 31.0 41.0 24-25 37.13524490770224 39.0 36.0 40.0 31.0 41.0 26-27 36.84845215008127 39.0 36.0 40.0 30.5 41.0 28-29 36.63143686704017 38.5 35.0 40.0 30.0 41.0 30-31 36.38219834797644 38.0 35.0 40.0 30.0 41.0 32-33 36.37343728933138 38.0 35.0 40.0 30.0 41.0 34-35 36.23697956636289 38.0 35.0 40.0 30.0 41.0 36-37 36.29123211516169 38.0 35.0 40.0 30.0 41.0 38-39 36.21084436194596 38.0 35.0 40.0 30.0 41.0 40-41 36.140868249382464 38.0 35.0 40.0 30.0 41.0 42-43 35.93360233905236 38.0 35.0 40.0 29.0 41.0 44-45 35.71362932159511 38.0 34.5 40.0 28.0 41.0 46-47 35.46181147665399 38.0 34.0 40.0 27.0 41.0 48-49 35.18323474218613 38.0 34.0 40.0 26.5 41.0 50-51 34.9801709685817 38.0 34.0 40.0 26.0 41.0 52-53 34.755259772692 38.0 33.0 40.0 26.0 41.0 54-55 34.51170543746571 38.0 33.0 40.0 25.0 41.0 56-57 34.16929913226788 37.5 33.0 40.0 24.5 41.0 58-59 33.83021137455789 37.0 32.5 40.0 23.0 41.0 60-61 33.541709100714755 37.0 32.0 39.5 23.0 41.0 62-63 33.26503946048482 36.0 32.0 39.0 22.5 41.0 64-65 32.84388962427106 36.0 31.0 39.0 22.0 40.0 66-67 31.968319679533543 35.0 30.5 38.5 16.5 40.0 68-69 30.068043155108917 34.0 27.0 38.0 9.5 40.0 70-71 29.4260617274424 34.0 26.5 37.0 9.0 39.0 72-73 30.01426610790812 34.0 28.0 36.5 10.5 39.0 74-75 30.11266017174872 34.0 28.5 36.0 12.5 39.0 76-77 29.613943156889665 33.5 28.5 35.5 12.5 38.0 78-79 30.13894186372883 34.0 29.0 36.0 13.0 38.0 80-81 29.948319145309725 34.0 29.0 35.5 9.5 37.0 82-83 29.49443114066961 34.0 29.0 35.0 7.5 37.0 84-85 29.132854468103872 34.0 29.0 35.0 6.5 36.0 86-87 28.745393803512478 33.5 29.0 35.0 2.0 36.0 88-89 28.289625415825405 33.0 27.5 35.0 2.0 35.5 90-91 27.808075852998563 33.0 26.5 35.0 2.0 35.0 92-93 27.32610124304555 33.0 26.0 35.0 2.0 35.0 94-95 26.517539076776444 32.0 25.0 34.0 2.0 35.0 96-97 26.026831815317976 32.0 24.0 34.0 2.0 35.0 98-99 25.372321779117954 32.0 22.0 34.0 2.0 35.0 100-101 23.894799882301164 31.0 10.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3379906485908002 1101 2 0.37824502343056565 1101 3 0.45003926520434234 1101 4 0.25801604063426 1101 5 0.3308414939686557 1101 6 0.2538464505296787 1101 7 0.1875972184113195 1101 8 0.33690044713194567 1101 9 0.4604219329624044 1101 10-11 0.4937976881095736 1101 12-13 0.5327134265330855 1101 14-15 0.4435792034187358 1101 16-17 0.44753821350762024 1101 18-19 0.32047070539115907 1101 20-21 0.34171118531665456 1101 22-23 0.4196431916862622 1101 24-25 0.35002544045667605 1101 26-27 0.245673956970073 1101 28-29 0.22910919521422812 1101 30-31 0.27017425141591644 1101 32-33 0.11940028327632035 1101 34-35 0.24144517339981064 1101 36-37 0.4175576064640367 1101 38-39 0.3661314267267102 1101 40-41 0.33017459846344366 1101 42-43 0.2727913960560713 1101 44-45 0.23513963449195074 1101 46-47 0.13481112701790465 1101 48-49 0.27396212082644666 1101 50-51 0.24140995937184329 1101 52-53 0.0351098637768672 1101 54-55 -0.06397640982006436 1101 56-57 -0.030302364722068376 1101 58-59 0.014616112344214116 1101 60-61 -0.01246004280960733 1101 62-63 -0.03606057283851527 1101 64-65 -0.15064437361112937 1101 66-67 -0.2061374265254159 1101 68-69 -9.101511987415883 1101 70-71 -6.563503897830746 1101 72-73 -1.5246218854163445 1101 74-75 -0.4319047445453812 1101 76-77 -0.3071023185012969 1101 78-79 -0.5136836149340169 1101 80-81 -0.4435054254989552 1101 82-83 -0.5272794041905122 1101 84-85 -0.2277679561220971 1101 86-87 -0.46525493585553335 1101 88-89 -0.43797305631449746 1101 90-91 -0.5805595404089345 1101 92-93 -0.7407257879768814 1101 94-95 -0.5952036581653779 1101 96-97 -1.1615725900049938 1101 98-99 -0.9746888231837971 1101 100-101 -0.8805728703824123 1102 1 0.28470225477902744 1102 2 0.21496819801746625 1102 3 0.2644988539514941 1102 4 0.23247857316263065 1102 5 0.33575347408324774 1102 6 0.3029338370059875 1102 7 0.23791123552328486 1102 8 0.27231587171863936 1102 9 0.3639467802124088 1102 10-11 0.43308765800197335 1102 12-13 0.43964512171280745 1102 14-15 0.4953823107617339 1102 16-17 0.5159706252003744 1102 18-19 0.4385699157797305 1102 20-21 0.39138914722776263 1102 22-23 0.47336149332296173 1102 24-25 0.43780957529509124 1102 26-27 0.4884499922756049 1102 28-29 0.41715612040993477 1102 30-31 0.42913082981601036 1102 32-33 0.25203044429949983 1102 34-35 0.10923499734350628 1102 36-37 0.24447832034751826 1102 38-39 0.32736877404907005 1102 40-41 0.43151525927618195 1102 42-43 0.2535795025184129 1102 44-45 0.1502526434415401 1102 46-47 0.13704550657880787 1102 48-49 0.04215886464057661 1102 50-51 0.07305056337620641 1102 52-53 0.13495490181036018 1102 54-55 0.08291446933689173 1102 56-57 -0.11690146223812548 1102 58-59 -0.19982544987748696 1102 60-61 -0.018092918561372073 1102 62-63 0.05184850732081969 1102 64-65 0.003684597247918475 1102 66-67 0.2632426181109082 1102 68-69 -4.33226689364901 1102 70-71 0.5313703671542171 1102 72-73 0.7188456904046276 1102 74-75 0.33786105119754595 1102 76-77 0.22445501618595287 1102 78-79 0.0649635522140457 1102 80-81 -0.061538070048122506 1102 82-83 0.06724946264390752 1102 84-85 0.01637147987919363 1102 86-87 -0.1666315670286913 1102 88-89 -0.17074362577905333 1102 90-91 -0.14185948442747787 1102 92-93 -0.26403094314970943 1102 94-95 0.01784655397706203 1102 96-97 -0.3747871908290854 1102 98-99 -0.3283996826769524 1102 100-101 -0.39283950750101226 1103 1 0.1670114842840782 1103 2 0.2436673237426099 1103 3 0.19180754869429606 1103 4 0.16853705928264162 1103 5 0.21750353718191207 1103 6 0.07673808650688585 1103 7 0.15672515414803456 1103 8 0.19019571711093164 1103 9 0.32836371656685515 1103 10-11 0.2723607285707672 1103 12-13 0.35657566174734256 1103 14-15 0.3081247861327796 1103 16-17 0.256405464707008 1103 18-19 0.30544670934201434 1103 20-21 0.31611050989041445 1103 22-23 0.13899152923965374 1103 24-25 0.23424843542765927 1103 26-27 0.1799611288427485 1103 28-29 0.14873225065411333 1103 30-31 0.21378977024644286 1103 32-33 0.06810113904983695 1103 34-35 -0.22475686608100887 1103 36-37 0.12492079557115687 1103 38-39 0.19725620536733857 1103 40-41 0.18165017823554308 1103 42-43 0.03143082944126263 1103 44-45 0.1449249209898582 1103 46-47 -0.05420529167464139 1103 48-49 -0.1602577366071145 1103 50-51 -0.15333845494934195 1103 52-53 -0.09700735462099885 1103 54-55 -0.12068458705751084 1103 56-57 -0.12055741799093056 1103 58-59 0.020662839648203146 1103 60-61 -0.2488461864664515 1103 62-63 -0.28928755044464083 1103 64-65 -0.460261862978129 1103 66-67 -0.2056466667768433 1103 68-69 -4.446195165407158 1103 70-71 0.0427825047340491 1103 72-73 0.38218730422700986 1103 74-75 0.10406512531608669 1103 76-77 -0.09931617305158369 1103 78-79 -0.17734090783413592 1103 80-81 -0.2068368219801826 1103 82-83 -0.44682887011644823 1103 84-85 -0.4062525174006453 1103 86-87 -0.5018343844149982 1103 88-89 -0.5448543685711869 1103 90-91 -0.6489655165620221 1103 92-93 -0.6367341884277096 1103 94-95 -0.712368921632951 1103 96-97 -1.2778719746401883 1103 98-99 -0.8488127779152457 1103 100-101 -0.797133689304129 1104 1 0.1990211715294734 1104 2 0.23210665600776537 1104 3 0.2117882126195525 1104 4 0.22491356211022406 1104 5 0.26851716047099927 1104 6 0.17135936035084143 1104 7 0.1311924116747747 1104 8 0.14769620931647154 1104 9 0.29795213669109444 1104 10-11 0.29338190701489 1104 12-13 0.2454074779137727 1104 14-15 0.3127928155018438 1104 16-17 0.32916464894234565 1104 18-19 0.24920970476889437 1104 20-21 0.3557649095677249 1104 22-23 0.2638598342438243 1104 24-25 0.19549185067786823 1104 26-27 0.2270042140351265 1104 28-29 0.23168024973852397 1104 30-31 0.2812492213132103 1104 32-33 0.18776415032333915 1104 34-35 0.11900228774163679 1104 36-37 0.22487357585220025 1104 38-39 0.29603897705278825 1104 40-41 0.17202423844308612 1104 42-43 0.20410629871722819 1104 44-45 0.1884182037847566 1104 46-47 0.07398303977256404 1104 48-49 0.11113351638013569 1104 50-51 0.24741433941758117 1104 52-53 0.0023112408244543303 1104 54-55 -0.06814472641170255 1104 56-57 0.16117311267867507 1104 58-59 -0.05865308268095504 1104 60-61 -0.04301166997235839 1104 62-63 0.16238418767319018 1104 64-65 0.21996972173164409 1104 66-67 0.22102006925212692 1104 68-69 -4.143381830558525 1104 70-71 0.6524424587308388 1104 72-73 0.928725778693952 1104 74-75 0.5619167241437566 1104 76-77 0.3425837243455696 1104 78-79 0.13937191143919136 1104 80-81 0.07825863388218579 1104 82-83 0.07623756242578139 1104 84-85 0.12047800526562114 1104 86-87 0.01608501872775392 1104 88-89 0.017185619327758417 1104 90-91 0.04837785402953898 1104 92-93 0.09069982801755927 1104 94-95 0.4531845334899067 1104 96-97 0.01574127825422167 1104 98-99 0.18554933170172916 1104 100-101 0.09207164284257985 1105 1 0.15662765273895118 1105 2 0.08897952324745262 1105 3 0.025625410658800973 1105 4 0.046255376994963626 1105 5 0.18702058797263277 1105 6 0.20860598961696297 1105 7 0.07033627464458192 1105 8 0.09918098365078265 1105 9 0.14093064116661935 1105 10-11 0.12374836465979655 1105 12-13 0.13929799793809394 1105 14-15 0.10090572853683 1105 16-17 0.24918405319063908 1105 18-19 0.1488656842785261 1105 20-21 0.17511620109353032 1105 22-23 0.19731279005707592 1105 24-25 0.1055483167228104 1105 26-27 0.10706285551543004 1105 28-29 0.3071522159986344 1105 30-31 0.16732778470015575 1105 32-33 0.10209463230136606 1105 34-35 0.20622890935402438 1105 36-37 0.011047493176135958 1105 38-39 0.12346272169160954 1105 40-41 0.06254140453360435 1105 42-43 0.09375519615260686 1105 44-45 -0.027248344847336625 1105 46-47 0.1156414360797271 1105 48-49 0.09052213964712763 1105 50-51 0.07053731185624201 1105 52-53 0.04206898942352666 1105 54-55 0.08268805247672617 1105 56-57 0.03535506164644886 1105 58-59 -0.011320293471122511 1105 60-61 0.1654317980844695 1105 62-63 0.0605183151727644 1105 64-65 -0.01197805511633021 1105 66-67 0.5345585022082275 1105 68-69 1.7011264654721785 1105 70-71 1.99741116898349 1105 72-73 1.1124200172836325 1105 74-75 0.5932592694666496 1105 76-77 0.2835697205758727 1105 78-79 0.1639918851116562 1105 80-81 0.1868751840227958 1105 82-83 -0.04849269152850866 1105 84-85 -0.21044557026089095 1105 86-87 -0.12571120808281933 1105 88-89 -0.014384870335746314 1105 90-91 -0.011467420115014448 1105 92-93 -0.07793277588329772 1105 94-95 0.31884790630395443 1105 96-97 -0.13989468725004528 1105 98-99 0.07475718740526105 1105 100-101 0.09086205314239137 1106 1 0.4282451941075145 1106 2 0.3667384616969329 1106 3 0.3469342697731541 1106 4 0.2882452333183494 1106 5 0.41261267055036654 1106 6 0.46503640102925914 1106 7 0.3346215942281674 1106 8 0.3860931483198371 1106 9 0.5274212807637753 1106 10-11 0.493910177414115 1106 12-13 0.4806245531442457 1106 14-15 0.6334484987108553 1106 16-17 0.49816164872240876 1106 18-19 0.6653280380098963 1106 20-21 0.5833576150206667 1106 22-23 0.5047299819715008 1106 24-25 0.5818969257001498 1106 26-27 0.723294568840096 1106 28-29 0.6860440839088042 1106 30-31 0.6961008136795215 1106 32-33 0.820954020425738 1106 34-35 0.6957693870645301 1106 36-37 0.6546751449655517 1106 38-39 0.9459951573883387 1106 40-41 0.8553942560548933 1106 42-43 0.8716935141202669 1106 44-45 0.7884140904446113 1106 46-47 0.8482626595992073 1106 48-49 0.8969277184173308 1106 50-51 0.9671904394002908 1106 52-53 0.8807563310912911 1106 54-55 0.8590375684291587 1106 56-57 0.9092489089457771 1106 58-59 1.0938296958551277 1106 60-61 1.066511551931427 1106 62-63 0.8648022440612166 1106 64-65 0.9215688876469983 1106 66-67 1.3649333331791382 1106 68-69 2.8418141742968963 1106 70-71 3.1577647011559975 1106 72-73 1.982780440787252 1106 74-75 1.673154453378526 1106 76-77 1.151660606870255 1106 78-79 0.8689114859687166 1106 80-81 0.9643069396057697 1106 82-83 1.0524683325605793 1106 84-85 1.022141137817588 1106 86-87 1.037769624594219 1106 88-89 1.262942415153308 1106 90-91 1.2220838727695806 1106 92-93 1.0419889823987702 1106 94-95 1.2001580385426003 1106 96-97 0.9760373883775735 1106 98-99 1.1480794052549683 1106 100-101 1.434348972630044 1107 1 0.11223860482658665 1107 2 0.07101204455458543 1107 3 0.14072297860611016 1107 4 0.1696131476066185 1107 5 0.09816503362485207 1107 6 0.09532410742315989 1107 7 -0.007830486630993505 1107 8 -0.01729956516137321 1107 9 0.05518743791046177 1107 10-11 0.07585886785838625 1107 12-13 0.038335161120336636 1107 14-15 0.15203946526642653 1107 16-17 0.103307672806487 1107 18-19 0.1079126706830209 1107 20-21 0.1257987137449561 1107 22-23 0.009585617977570848 1107 24-25 0.1169498570362677 1107 26-27 0.17441819447699203 1107 28-29 0.18140579201386942 1107 30-31 0.1331639864948997 1107 32-33 0.17521113008108102 1107 34-35 0.15407532891029518 1107 36-37 -0.01033347473332924 1107 38-39 0.10683337521770397 1107 40-41 0.09354542641845143 1107 42-43 0.09623475459071074 1107 44-45 0.10346548938981215 1107 46-47 0.11387478591929323 1107 48-49 0.17679491120487256 1107 50-51 0.21389669283220059 1107 52-53 0.19476647041116735 1107 54-55 0.2281706182701697 1107 56-57 0.19199648585264129 1107 58-59 0.2403484867987018 1107 60-61 0.22505424817323672 1107 62-63 0.3026461700467351 1107 64-65 0.2886590050026001 1107 66-67 0.8076372369449842 1107 68-69 2.120050903153089 1107 70-71 2.4019080307860534 1107 72-73 1.4554367127355619 1107 74-75 0.6525614180952211 1107 76-77 0.2735676673613874 1107 78-79 0.3813933207514353 1107 80-81 0.1857989775579867 1107 82-83 0.3892932185890068 1107 84-85 0.27884011239266826 1107 86-87 0.3848382884770416 1107 88-89 0.293806811327439 1107 90-91 0.3478474503915656 1107 92-93 0.4846504900160298 1107 94-95 0.8169134561829772 1107 96-97 0.565570647645341 1107 98-99 0.8313221998251947 1107 100-101 0.8628917965710983 1108 1 -0.11059008285180028 1108 2 -0.19939219827450572 1108 3 -0.1406114073374951 1108 4 -0.07961581536984141 1108 5 -0.0601896555514827 1108 6 -0.009892386170086809 1108 7 -0.1567116833678952 1108 8 -0.1312098098932637 1108 9 -0.21768072265308547 1108 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0.11152782149855511 2316 68-69 1.611525272859506 2316 70-71 1.7761821789828502 2316 72-73 0.7993371707796655 2316 74-75 0.3533472620854887 2316 76-77 -0.03751966151599717 2316 78-79 -0.3601488326139233 2316 80-81 -0.39859172075536264 2316 82-83 -0.335733761797087 2316 84-85 -0.5017482617868687 2316 86-87 -0.3561534392550172 2316 88-89 -0.33981663091478964 2316 90-91 -0.4252011829885589 2316 92-93 -0.5633906523897565 2316 94-95 -0.06549112511663324 2316 96-97 -0.0414325762639578 2316 98-99 -0.07792960423323692 2316 100-101 -0.13592372799028496 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 16.0 4 38.0 5 68.0 6 180.0 7 534.0 8 1191.0 9 2090.0 10 3363.0 11 5279.0 12 7531.0 13 9691.0 14 11839.0 15 14316.0 16 17461.0 17 20432.0 18 19167.0 19 16688.0 20 16717.0 21 17643.0 22 18848.0 23 21920.0 24 26442.0 25 32125.0 26 39989.0 27 49507.0 28 61670.0 29 74843.0 30 89574.0 31 107310.0 32 128565.0 33 156026.0 34 194620.0 35 246066.0 36 315370.0 37 366372.0 38 240640.0 39 24421.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.73133244727997 20.01346891589646 14.43222327866322 23.822975358160356 2 15.382064295217024 21.21785419388821 39.799496769886936 23.600584741007825 3 18.79001645494859 25.244460499431643 30.71974818544019 25.245774860179576 4 12.756968016952703 17.484552027888178 37.925948098883985 31.832531856275136 5 12.986769056738808 38.23643389489019 35.149383395475716 13.627413652895282 6 31.13214746951744 37.65226438821621 17.606109146166183 13.609478996100165 7 27.293282941046282 33.255390360736754 21.605071225602718 17.846255472614246 8 25.66122917269082 36.25556589142028 20.094404980661945 17.988799955226956 9 25.998044571226025 16.41796993252668 20.475399841089615 37.108585655157675 10-11 24.365694011859766 27.08743717570282 28.27046734408508 20.276401468352326 12-13 25.174682709210106 24.340975560532222 28.511376847418045 21.97296488283963 14-15 22.03164470554516 25.864149426642165 26.556350861245114 25.54785500656756 16-17 21.533251051384383 29.07099552819796 27.337053969863778 22.058699450553874 18-19 21.514783160247642 27.82706157395905 29.384154412731146 21.274000853062162 20-21 22.382388082229767 27.013684609520546 29.31606207511187 21.28786523313782 22-23 21.87214073660137 27.115823115949468 29.126455017930418 21.885581129518748 24-25 21.62320756512929 27.242614503731833 29.40295947715522 21.731218453983654 26-27 21.667923438731883 27.39477616702257 29.1631466802456 21.774153713999947 28-29 21.61295451461909 27.585836272980124 29.160398717861746 21.64081049453904 30-31 21.769231845511797 27.363858033053372 29.11362028094392 21.75328984049091 32-33 21.525068756522938 27.311407424241686 29.08094052012219 22.082583299113185 34-35 21.905867547692058 27.43022577406312 28.9768255949635 21.687081083281324 36-37 21.950724660441757 27.140422896685035 29.141576988287817 21.76727545458539 38-39 21.638211828099145 27.35649799748362 29.172078897002134 21.8332112774151 40-41 21.876663347399894 27.233522726790387 29.15602336560068 21.733790560209037 42-43 21.662206994441256 27.187312175923882 29.02962229745561 22.120858532179252 44-45 21.70613703916393 27.313502012460578 29.090040155835712 21.890320792539782 46-47 21.80888233135893 27.420620866427203 28.667459313877046 22.103037488336817 48-49 21.673720698534833 27.52456002045652 28.683981848025493 22.117737432983162 50-51 21.723927484343676 27.60406852284746 28.55552245191059 22.11648154089828 52-53 21.842388440195222 27.51512909054032 28.419692131175644 22.222790338088817 54-55 21.55898164329174 27.48841436173666 28.57852290165561 22.37408109331599 56-57 21.548237608417793 27.65916386576096 28.34641695078656 22.44618157503469 58-59 21.532583231932463 27.430244364963407 28.47012008449613 22.567052318607995 60-61 21.53066234949108 27.52899082911142 28.44881467070794 22.491532150689554 62-63 21.505970394475334 27.429894901977413 28.479412341465228 22.584722362082033 64-65 21.29223420085935 27.42888592597178 28.38915091789537 22.889728955273497 66-67 21.079817259930746 27.562211045290773 28.402688832572554 22.955282862205927 68-69 21.089584842009902 27.382772371731097 28.68467244210407 22.842970344154935 70-71 21.11249231452875 27.169016095808274 28.4878631804156 23.23062840924738 72-73 21.08865481448196 27.22021410722435 28.348194044998152 23.34293703329554 74-75 21.275322033668274 27.389678127497003 27.882759047733764 23.452240791100962 76-77 21.112132777844458 27.31559846178837 28.03546352260982 23.536805237757353 78-79 21.29775598189996 27.279995149352455 27.845769760452637 23.576479108294947 80-81 21.255652343509848 27.248291842181693 27.807176912786964 23.688878901521495 82-83 21.1709642385684 27.22376636087987 27.836491792345374 23.768777608206364 84-85 21.142631216882425 27.081201459796763 27.72418193824801 24.051985385072804 86-87 21.09775557280264 27.059327831461154 27.75921278100919 24.08370381472701 88-89 21.15776968241531 26.96535840634423 27.643931530871484 24.232940380368973 90-91 21.051476765698578 26.955910825700286 27.678388772421354 24.31422363617978 92-93 21.063826314036564 26.79601939633744 27.673043708327356 24.46711058129864 94-95 20.735589615030715 26.650654125937834 27.96970502540741 24.644051233624044 96-97 20.914581733384956 26.320410627148664 27.996068102566884 24.76893953689949 98-99 20.794855185472215 26.396795728769213 27.788910787380956 25.019438298377615 100-101 20.729084267598743 26.271156794397015 27.94644225780743 25.053316680196808 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 306.0 1 214.0 2 164.5 3 240.5 4 235.5 5 238.5 6 376.5 7 706.0 8 1142.5 9 1419.5 10 1398.5 11 1307.5 12 1381.0 13 1523.0 14 1685.0 15 1862.0 16 2035.0 17 2262.5 18 2518.5 19 2897.5 20 3421.5 21 4096.0 22 5022.5 23 6393.0 24 8138.0 25 10375.5 26 13468.0 27 17647.0 28 21502.0 29 25074.5 30 29501.5 31 34858.5 32 40945.5 33 45681.0 34 49949.0 35 55379.0 36 62258.0 37 68059.0 38 71265.0 39 73711.0 40 76884.5 41 80044.0 42 81547.0 43 83760.5 44 86952.5 45 89977.5 46 91420.5 47 91748.5 48 92761.5 49 91891.5 50 89234.5 51 86598.0 52 83468.5 53 79828.5 54 75313.5 55 69247.5 56 61788.5 57 54010.5 58 47614.0 59 41118.0 60 33321.5 61 26551.5 62 20945.0 63 15364.0 64 10708.5 65 7527.5 66 5340.5 67 3971.5 68 2887.5 69 1997.5 70 1414.0 71 906.5 72 598.0 73 455.0 74 309.0 75 217.5 76 142.5 77 80.5 78 48.0 79 27.0 80 13.5 81 8.5 82 4.0 83 2.0 84 2.0 85 1.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022895306546955308 2 0.025227235917478528 3 4.2398715827695014E-5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.1199357913847505E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.4839550539693253E-4 26-27 2.7559165288001757E-4 28-29 8.479743165539003E-5 30-31 5.511833057600351E-4 32-33 0.0024803248759201584 34-35 0.004155074151114111 36-37 0.11008826564661009 38-39 0.10182051606020957 40-41 0.11108463546856091 42-43 0.11167821749014865 44-45 0.10561520112678828 46-47 0.09847101750982167 48-49 0.09940378925803095 50-51 0.09861941301521861 52-53 0.0781832319862696 54-55 0.06567561081709956 56-57 0.03572091808483304 58-59 0.014733553750124017 60-61 0.009751704640369853 62-63 0.0020987364334709028 64-65 0.006381006732068099 66-67 0.14472801647783692 68-69 0.4091052090214291 70-71 0.38843583505542784 72-73 0.17330475094570336 74-75 0.03112065741752814 76-77 0.032180625313220516 78-79 0.0048334536043572315 80-81 0.0011447653273477653 82-83 6.359807374154252E-5 84-85 6.147813795015776E-4 86-87 0.0013355595485723929 88-89 4.239871582769501E-4 90-91 5.299839478461876E-4 92-93 0.0032011030449909735 94-95 0.07917960180822044 96-97 0.01751066963683804 98-99 0.019482209922825858 100-101 0.010493682167354515 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2358562.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.762495981213654 #Duplication Level Percentage of deduplicated Percentage of total 1 69.86787150809751 36.86403289661946 2 15.29834019694984 16.143572263216104 3 6.2256858955142835 9.854481811271128 4 3.0906698755009456 6.52285827541507 5 1.6899274648856508 4.4582395537285855 6 1.0368765620197629 3.2824917261949422 7 0.6603737481829269 2.4390077064220472 8 0.458211722876109 1.934111534943655 9 0.3163251222386814 1.5021092691787812 >10 1.2719114190500858 11.123753298020437 >50 0.055580950890429096 2.011244111730656 >100 0.0262126238819431 2.541830971579207 >500 0.0016103279295480795 0.5845161734085709 >1k 2.4154918943195626E-4 0.24877728842777905 >5k 1.610327929546375E-4 0.48897311984353425 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6244 0.26473758162812766 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5266 0.22327163754864193 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3541 0.15013385274586802 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 8.479743165539002E-5 0.0 5 0.0 0.0 0.0 8.479743165539002E-5 0.0 6 0.0 0.0 0.0 8.479743165539002E-5 4.239871582769501E-5 7 0.0 0.0 0.0 8.479743165539002E-5 4.239871582769501E-5 8 0.0 0.0 0.0 8.479743165539002E-5 4.239871582769501E-5 9 0.0 0.0 0.0 1.2719614748308504E-4 4.239871582769501E-5 10-11 0.0 0.0 0.0 1.4839550539693253E-4 4.239871582769501E-5 12-13 0.0 0.0 0.0 1.6959486331078003E-4 1.2719614748308504E-4 14-15 0.0 0.0 0.0 1.6959486331078003E-4 1.4839550539693253E-4 16-17 0.0 0.0 0.0 3.8158844244925506E-4 1.6959486331078003E-4 18-19 0.0 0.0 0.0 4.8758523201849265E-4 2.1199357913847505E-4 20-21 0.0 0.0 0.0 5.511833057600351E-4 2.1199357913847505E-4 22-23 0.0 0.0 0.0 9.963698219508327E-4 2.1199357913847505E-4 24-25 0.0 0.0 0.0 0.001547553127710868 2.1199357913847505E-4 26-27 0.0 0.0 0.0 0.0028407139604555657 2.3319293705232258E-4 28-29 0.0 0.0 0.0 0.00824655022848668 2.543922949661701E-4 30-31 0.0 0.0 0.0 0.029891094658524985 2.543922949661701E-4 32-33 0.0 0.0 0.0 0.08280469201148835 2.543922949661701E-4 34-35 0.0 0.0 0.0 0.16679654806615218 2.543922949661701E-4 36-37 0.0 0.0 0.0 0.30408358991622864 2.543922949661701E-4 38-39 0.0 0.0 0.0 0.5118160981140203 2.543922949661701E-4 40-41 0.0 0.0 0.0 0.7445426492922382 2.543922949661701E-4 42-43 0.0 0.0 0.0 0.9760820364272806 2.543922949661701E-4 44-45 0.0 0.0 0.0 1.248938972136412 2.9679101079386507E-4 46-47 0.0 0.0 0.0 1.5745187109772818 3.6038908453540756E-4 48-49 0.0 0.0 0.0 1.9133904472301344 3.815884424492551E-4 50-51 0.0 0.0 0.0 2.27288915873316 3.815884424492551E-4 52-53 0.0 0.0 0.0 2.610107345068733 3.815884424492551E-4 54-55 0.0 0.0 0.0 2.9812021053506332 3.815884424492551E-4 56-57 0.0 0.0 0.0 3.3891625490447144 3.815884424492551E-4 58-59 0.0 0.0 0.0 3.810054601066243 3.815884424492551E-4 60-61 0.0 0.0 0.0 4.2351865246705405 3.815884424492551E-4 62-63 0.0 0.0 0.0 4.648913193717188 3.815884424492551E-4 64-65 0.0 0.0 0.0 5.078624178630878 3.815884424492551E-4 66-67 0.0 0.0 0.0 5.524425476201177 3.815884424492551E-4 68-69 0.0 0.0 0.0 6.023733953146027 3.815884424492551E-4 70-71 0.0 0.0 0.0 6.583460600145343 3.815884424492551E-4 72-73 0.0 0.0 0.0 7.152960151142942 3.815884424492551E-4 74-75 0.0 0.0 0.0 7.720869750297004 3.815884424492551E-4 76-77 0.0 0.0 0.0 8.328146557097078 3.815884424492551E-4 78-79 0.0 0.0 0.0 8.997473884510985 3.815884424492551E-4 80-81 0.0 0.0 0.0 9.691201672883732 3.815884424492551E-4 82-83 0.0 0.0 0.0 10.391162072483148 3.815884424492551E-4 84-85 0.0 0.0 0.0 11.06381769908953 3.815884424492551E-4 86-87 0.0 0.0 0.0 11.753093622300367 3.815884424492551E-4 88-89 0.0 0.0 0.0 12.489834907880311 4.027878003631026E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4220 0.0 42.512756 1 GTATCAA 7515 0.0 39.09323 1 ATCAACG 9360 0.0 30.964624 3 TCAACGC 9470 0.0 30.554863 4 TATCAAC 9645 0.0 30.20488 2 CAACGCA 9645 0.0 30.000471 5 AACGCAG 9970 0.0 29.070103 6 ACGCAGA 11345 0.0 25.337784 7 GTACATG 8935 0.0 25.01879 1 CGCAGAG 11410 0.0 24.985573 8 TACATGG 8915 0.0 24.588474 2 ACATGGG 8915 0.0 23.35843 3 GCAGAGT 12580 0.0 22.1339 9 GTACCGT 245 7.64885E-9 21.297424 6 GAGTACT 6645 0.0 21.022812 12-13 AGAGTAC 11270 0.0 20.224134 10-11 CATGGGA 5715 0.0 20.003283 4 AGTACTT 7285 0.0 19.76194 12-13 GTACTTT 7565 0.0 18.372116 14-15 CAGAGTA 12095 0.0 18.275974 10-11 >>END_MODULE