FastQCFastQC Report
Fri 27 May 2016
SRR939801_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939801_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054075
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97760.9274482366055545No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75850.719588264592178No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51630.4898133434527904No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29680.28157389180086806No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14630.1387946777980694No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14540.1379408486113417No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14150.13424092213552166No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10800.10245950240732396No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56250.042.2533341
AACGCCG651.3376263E-436.4941376
GTATCAA92350.034.4178471
ATGGGCG702.0679059E-433.8874135
CGCAGAA2800.033.88589
TCCGTCG300.003823093631.88138488-89
CATTCCG2300.030.9392099
CACACGG650.005878614729.1953095
TATCAAC114350.028.2096612
TCAACGC115500.028.0122624
ATCAACG115750.027.9107743
ACGCGGA856.4524484E-427.907287
AACGCAG116600.027.383126
CAACGCA116700.027.3596575
AGTGCGA700.0084410827.109936
GCGCAGA1056.964835E-527.108641
CGCCGAG909.0131693E-426.3556238
GTACGCT659.615369E-625.71522592-93
GTACATG60500.025.4843641
ACGCAGA128050.024.7863737
Produced by FastQC (version 0.11.5)