FastQCFastQC Report
Fri 27 May 2016
SRR939801_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939801_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054075
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105110.9971776201883168No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85980.8156914830538623No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62530.5932215449564784No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34230.32473970068543506No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17700.1679197400564476No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT17300.16412494367099115No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16300.15463795270735003No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15680.14875601830989255No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12040.11422337120223892No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50700.043.6163181
GTATCAA87600.036.6544841
CTGGTCG3050.029.3472169
TCAACGC108600.029.341164
ATCAACG108850.029.2737713
CAACGCA109700.029.0039795
AACGCAG109400.028.9808376
TATCAAC110350.028.8772222
AGGGCGC350.00753801227.74724662-63
TTACCGA457.738116E-427.05453564-65
GTACATG62050.027.051561
GCCGGCG458.416478E-426.66811688-89
ACGCAGA122600.025.7836977
CGCAGAG122050.025.7068948
CGGTCGC959.149517E-925.62998468-69
CATGGGG21050.025.0799314
ACATGGG62850.025.0496483
CGCAGAA1701.2158489E-724.940599
TACATGG66200.024.7088492
GGTCGCG500.001430955824.34848468-69
Produced by FastQC (version 0.11.5)