##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939801_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1054075 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.37178094537865 31.0 31.0 34.0 30.0 34.0 2 31.570623532481086 31.0 31.0 34.0 30.0 34.0 3 31.5915717572279 31.0 31.0 34.0 30.0 34.0 4 35.443439982923415 37.0 35.0 37.0 33.0 37.0 5 35.19185731565591 37.0 35.0 37.0 32.0 37.0 6 35.22798757204184 37.0 35.0 37.0 32.0 37.0 7 35.15330028698148 37.0 35.0 37.0 32.0 37.0 8 35.10816213267557 37.0 35.0 37.0 32.0 37.0 9 36.64891587410763 39.0 35.0 39.0 32.0 39.0 10-11 36.525077911913286 38.0 35.0 39.0 32.0 39.0 12-13 36.4806783198539 38.0 35.0 39.0 32.0 39.0 14-15 37.39426179351564 39.0 36.0 41.0 32.0 41.0 16-17 37.349682422977494 39.0 36.0 41.0 32.0 41.0 18-19 37.46028271233072 39.0 36.0 41.0 32.0 41.0 20-21 37.45803619287052 39.0 36.0 41.0 32.0 41.0 22-23 37.29550174323459 39.0 36.0 41.0 31.5 41.0 24-25 37.13450513483386 39.0 36.0 40.5 31.0 41.0 26-27 36.82075753622844 39.0 35.5 40.0 31.0 41.0 28-29 36.60107297867799 38.5 35.0 40.0 30.0 41.0 30-31 36.26138747242843 38.0 35.0 40.0 30.0 41.0 32-33 36.052452624338876 38.0 35.0 40.0 30.0 41.0 34-35 35.97048834285985 38.0 35.0 40.0 30.0 41.0 36-37 35.832470175272164 38.0 35.0 40.0 30.0 41.0 38-39 35.63317411000166 38.0 35.0 40.0 29.0 41.0 40-41 35.414326779403744 38.0 34.5 40.0 27.5 41.0 42-43 35.11041149823305 38.0 34.0 40.0 26.5 41.0 44-45 34.677816569029716 38.0 34.0 40.0 24.5 41.0 46-47 34.134124706496216 38.0 33.0 40.0 23.5 41.0 48-49 33.672630979768996 38.0 33.0 40.0 19.0 41.0 50-51 33.374154590517755 38.0 33.0 40.0 15.0 41.0 52-53 33.129323814719065 38.0 33.0 40.0 11.0 41.0 54-55 32.84525863909114 38.0 32.5 40.0 8.5 41.0 56-57 31.541025069373624 36.5 30.0 40.0 2.0 41.0 58-59 28.381767900766075 34.5 21.5 39.0 2.0 40.0 60-61 28.335245120129024 34.0 19.5 39.0 2.0 40.0 62-63 30.21037497331784 35.5 28.5 39.0 2.0 40.0 64-65 29.85924625856794 35.0 28.0 39.0 2.0 40.0 66-67 24.61375566254773 28.5 16.5 36.0 2.0 39.0 68-69 17.168932476341816 13.0 7.0 29.0 2.0 36.5 70-71 16.114832910371653 13.0 6.5 25.5 2.0 33.5 72-73 18.169585181320116 16.5 7.0 30.0 2.0 35.0 74-75 18.79256931432773 17.5 7.0 31.0 2.0 35.5 76-77 19.607764627754193 22.0 7.0 31.5 2.0 35.0 78-79 22.466326399924103 27.5 8.0 34.0 2.0 36.0 80-81 24.86087659796504 31.5 15.0 35.0 2.0 37.0 82-83 25.745764770059054 32.5 20.0 35.0 2.0 37.0 84-85 25.65778478760999 33.0 19.5 35.0 2.0 36.0 86-87 25.33435856082347 33.0 18.0 35.0 2.0 36.0 88-89 24.85168939591585 32.0 9.5 35.0 2.0 35.5 90-91 23.213880890828452 31.5 2.0 34.5 2.0 35.0 92-93 21.16010910039608 30.0 2.0 34.0 2.0 35.0 94-95 17.07958541849489 18.0 2.0 33.0 2.0 35.0 96-97 15.8576647771743 9.5 2.0 32.0 2.0 34.5 98-99 14.821693902236557 2.0 2.0 31.5 2.0 34.0 100-101 14.217162915352322 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.070647484857151 1101 2 0.04989906657545973 1101 3 0.359005028044578 1101 4 0.1598600557047547 1101 5 0.3263998765601954 1101 6 0.3112835225673578 1101 7 0.41107351458215646 1101 8 0.451931950205406 1101 9 0.4314986650268935 1101 10-11 0.34739234775589267 1101 12-13 0.3572001955935562 1101 14-15 0.4980566250946268 1101 16-17 0.5587847228446563 1101 18-19 0.3423821605509332 1101 20-21 0.4697928891269285 1101 22-23 0.40724852490765073 1101 24-25 0.35179473030166974 1101 26-27 0.5476370180399144 1101 28-29 0.5106039635987756 1101 30-31 0.5564344812935147 1101 32-33 0.4004960202539607 1101 34-35 0.7361514668672555 1101 36-37 0.5488691265577543 1101 38-39 0.5773856808202851 1101 40-41 0.33684759992228663 1101 42-43 0.5491313468900216 1101 44-45 0.42543463867233555 1101 46-47 -0.11042709224371094 1101 48-49 -0.08866931753170348 1101 50-51 0.0049589109838024115 1101 52-53 -0.12638536723455474 1101 54-55 0.013135297920484845 1101 56-57 0.733977560039488 1101 58-59 -8.117913847634124 1101 60-61 -9.71381825407579 1101 62-63 -2.6863369642233366 1101 64-65 -0.4867452572478115 1101 66-67 -1.9628064546635606 1101 68-69 -3.8610357733890233 1101 70-71 -4.399256892409857 1101 72-73 -6.724172009342652 1101 74-75 -7.415824535951941 1101 76-77 -8.63075473943167 1101 78-79 -11.255478105923046 1101 80-81 -6.837294556021721 1101 82-83 -3.246043550477207 1101 84-85 -2.267330728004602 1101 86-87 -2.201494730477858 1101 88-89 -2.324717422784282 1101 90-91 -7.624818660761569 1101 92-93 -12.81788713926614 1101 94-95 -11.75707841285064 1101 96-97 -11.890353371267175 1101 98-99 -11.468415381078172 1101 100-101 -11.360562254894424 1102 1 0.49082843435135004 1102 2 0.5903902775065752 1102 3 0.6453492071536111 1102 4 0.5038516297124005 1102 5 0.4708454597832272 1102 6 0.5167826601242993 1102 7 0.6184131121668841 1102 8 0.6514190637142505 1102 9 0.6946822683597773 1102 10-11 0.712867585500156 1102 12-13 0.7145372854424608 1102 14-15 0.9408529904041316 1102 16-17 0.9696030951714647 1102 18-19 0.8485272036294731 1102 20-21 0.8230260147906634 1102 22-23 0.8616535264231899 1102 24-25 0.7704426899860053 1102 26-27 0.7581701626752135 1102 28-29 0.982687412324303 1102 30-31 0.8365365788509891 1102 32-33 0.8723134309186023 1102 34-35 0.8343129694153291 1102 36-37 0.8160171331258468 1102 38-39 0.7490554621675329 1102 40-41 1.0564042322293332 1102 42-43 0.9664534775495923 1102 44-45 0.8396242801969507 1102 46-47 0.7758209206482292 1102 48-49 0.8106810698589655 1102 50-51 0.833477754983015 1102 52-53 0.7506723390376209 1102 54-55 0.6990446574447517 1102 56-57 1.2381785626976871 1102 58-59 -7.971875307005536 1102 60-61 -8.964088108256398 1102 62-63 -2.289641505841942 1102 64-65 0.5184751945371495 1102 66-67 -0.6418816889187973 1102 68-69 -3.73336585248083 1102 70-71 -4.389631548141342 1102 72-73 -6.916460830728624 1102 74-75 -7.373550651948898 1102 76-77 -8.126897187548796 1102 78-79 -11.045519559886468 1102 80-81 -6.991603173256191 1102 82-83 -3.0039129345118702 1102 84-85 -2.254865100340133 1102 86-87 -2.0873131573206614 1102 88-89 -2.213912570424501 1102 90-91 -7.242236303446287 1102 92-93 -12.679896249979407 1102 94-95 -11.122568915073803 1102 96-97 -11.280233239818205 1102 98-99 -10.926588528243013 1102 100-101 -10.848196936662749 1103 1 0.23584944841729794 1103 2 0.267429540367079 1103 3 0.23177442003957793 1103 4 0.16532051791357816 1103 5 0.2431158773691564 1103 6 0.23048522873197896 1103 7 0.2925373597035943 1103 8 0.26236933874395163 1103 9 0.19633843631257974 1103 10-11 0.15615206903331114 1103 12-13 0.18045955835578553 1103 14-15 0.39498442564985936 1103 16-17 0.29466473975921303 1103 18-19 0.3553475408528257 1103 20-21 0.30054246166650955 1103 22-23 0.48668721880921595 1103 24-25 0.5501648650666127 1103 26-27 0.37906593091152985 1103 28-29 0.32057712092260005 1103 30-31 0.05520265985247619 1103 32-33 0.2373881531435984 1103 34-35 0.2912396248345672 1103 36-37 0.05213437264625753 1103 38-39 0.19701944375900382 1103 40-41 0.3307905316573141 1103 42-43 0.446544521863828 1103 44-45 0.31414784490131353 1103 46-47 0.15483830092795614 1103 48-49 0.1284741437026966 1103 50-51 -0.09472302168887836 1103 52-53 0.04471003457824452 1103 54-55 0.07390913918098363 1103 56-57 0.6847708898183136 1103 58-59 -6.801873510078281 1103 60-61 -8.820126282977625 1103 62-63 -2.76760868197076 1103 64-65 -0.3294508519234043 1103 66-67 -1.6883557183285056 1103 68-69 -3.750139669254148 1103 70-71 -4.4928964651332866 1103 72-73 -7.325761426709496 1103 74-75 -7.735382918772629 1103 76-77 -8.485070710823994 1103 78-79 -11.319223026957776 1103 80-81 -7.7030710631644475 1103 82-83 -4.0904304894928565 1103 84-85 -3.1386458352833806 1103 86-87 -2.8631115420827165 1103 88-89 -3.3701863422746356 1103 90-91 -8.202545674421916 1103 92-93 -13.328029442220673 1103 94-95 -11.514342482273891 1103 96-97 -11.813358096313598 1103 98-99 -11.290240683058279 1103 100-101 -11.055469500578454 1104 1 0.24314121784283316 1104 2 0.2844398326955151 1104 3 0.2552922260965964 1104 4 0.2845125865729443 1104 5 0.12133921760788269 1104 6 0.2642378182555092 1104 7 0.1883826130658619 1104 8 0.255505046218218 1104 9 0.39006274659504214 1104 10-11 0.3428115014755164 1104 12-13 0.32704220937021233 1104 14-15 0.4302372946775179 1104 16-17 0.43997811320961233 1104 18-19 0.5508869838175414 1104 20-21 0.48125667691757457 1104 22-23 0.39287659107582584 1104 24-25 0.44825108386902457 1104 26-27 0.4524152124215064 1104 28-29 0.3836340739987847 1104 30-31 0.3882441579945919 1104 32-33 0.2852071988836258 1104 34-35 0.337906949158004 1104 36-37 0.43076463801076414 1104 38-39 0.28951064759972667 1104 40-41 0.5229931885906609 1104 42-43 0.47258937405545964 1104 44-45 0.21609122065350306 1104 46-47 0.40511205055802435 1104 48-49 0.4243818744580139 1104 50-51 0.14237621871755124 1104 52-53 0.11466662923149329 1104 54-55 -0.0635386593129823 1104 56-57 0.6692962260453363 1104 58-59 -6.617581642004229 1104 60-61 -8.201831915807851 1104 62-63 -2.1223873804680125 1104 64-65 -0.0015768918431717793 1104 66-67 -1.604102487589632 1104 68-69 -3.4714411719998637 1104 70-71 -4.223190602123191 1104 72-73 -7.153494812655371 1104 74-75 -7.927255103179588 1104 76-77 -8.64869653700531 1104 78-79 -11.463849149084638 1104 80-81 -7.79491858544257 1104 82-83 -3.969707724703799 1104 84-85 -2.992051675275487 1104 86-87 -2.7544707542099083 1104 88-89 -2.8018895088919784 1104 90-91 -8.183493357825949 1104 92-93 -13.631093300692937 1104 94-95 -11.522333834087378 1104 96-97 -11.710984777448715 1104 98-99 -11.028891171368429 1104 100-101 -10.564074117328971 1105 1 0.13581254990497982 1105 2 0.19120727714651053 1105 3 0.10440075551414196 1105 4 0.1438774506408791 1105 5 -0.02455431701891797 1105 6 0.04593564216303747 1105 7 0.024820203572566868 1105 8 0.2052521188791374 1105 9 0.20958500223513 1105 10-11 0.09372230199709719 1105 12-13 0.16971370524540674 1105 14-15 0.2640383142499019 1105 16-17 0.2094333693149224 1105 18-19 0.13062134458628094 1105 20-21 0.18251082957822007 1105 22-23 0.2182379626496811 1105 24-25 0.399977018094809 1105 26-27 0.2675988796058206 1105 28-29 0.33539522802891497 1105 30-31 0.21758127213296774 1105 32-33 0.11192985684854051 1105 34-35 0.15072925132098902 1105 36-37 0.1040681901328 1105 38-39 0.22169532387238178 1105 40-41 0.07150887860395727 1105 42-43 0.2230971944901583 1105 44-45 0.28515316164149596 1105 46-47 -4.0631943667079895E-4 1105 48-49 0.13037022407436183 1105 50-51 0.2806254176717644 1105 52-53 0.48941845247067306 1105 54-55 0.4798447680490341 1105 56-57 1.0456878178494442 1105 58-59 -5.416812375952187 1105 60-61 -7.732478059976437 1105 62-63 -2.265665639856138 1105 64-65 0.00209989191706228 1105 66-67 -1.2009381110293127 1105 68-69 -3.3037850745507598 1105 70-71 -4.405779077547958 1105 72-73 -5.726619317120914 1105 74-75 -5.58352373691328 1105 76-77 -8.002839860313285 1105 78-79 -10.833693952607273 1105 80-81 -6.965551984956374 1105 82-83 -3.165809957147953 1105 84-85 -2.2562665521208416 1105 86-87 -2.2978263333545392 1105 88-89 -2.4744657311010982 1105 90-91 -7.723532541905943 1105 92-93 -10.65382317953681 1105 94-95 -11.108164497268104 1105 96-97 -11.333573449449133 1105 98-99 -10.84193298300964 1105 100-101 -10.394254868641113 1106 1 0.009735605850519136 1106 2 7.510467789622055E-4 1106 3 0.099497061835919 1106 4 0.04695837757870436 1106 5 -0.007499967326474177 1106 6 -0.05413977337996556 1106 7 -0.06944922518835739 1106 8 -0.03736708986367887 1106 9 0.049476794397975254 1106 10-11 -0.012081664725791086 1106 12-13 0.08078733303602803 1106 14-15 -0.06426507170597517 1106 16-17 -0.13743183550224103 1106 18-19 0.0656149982336629 1106 20-21 -0.07088792000514132 1106 22-23 0.08123923192020044 1106 24-25 -0.032887156492172664 1106 26-27 0.2270815424478414 1106 28-29 0.20251910142507512 1106 30-31 0.1351822161739591 1106 32-33 0.10870617906144986 1106 34-35 0.10958064566684556 1106 36-37 0.0430960703163592 1106 38-39 -0.02673323841781894 1106 40-41 0.08645731659319722 1106 42-43 -0.14788330857192022 1106 44-45 -0.020363956676739292 1106 46-47 -0.08518231170657486 1106 48-49 0.20207054156033877 1106 50-51 0.3066294712572031 1106 52-53 0.3062100261304437 1106 54-55 -0.0956061259954879 1106 56-57 0.4984512588182639 1106 58-59 -6.4075428293544725 1106 60-61 -8.419469888837021 1106 62-63 -2.1866931092943247 1106 64-65 0.014732837062354065 1106 66-67 -1.5895848733794473 1106 68-69 -3.616909399093469 1106 70-71 -4.337101273621636 1106 72-73 -5.791655964361347 1106 74-75 -6.139281578951385 1106 76-77 -8.239730517876218 1106 78-79 -11.554645501487656 1106 80-81 -7.505415327952324 1106 82-83 -3.9049193130520834 1106 84-85 -2.9920739529443523 1106 86-87 -2.8068703066762914 1106 88-89 -2.968177455187945 1106 90-91 -7.774866454322908 1106 92-93 -10.668804443487844 1106 94-95 -11.00868752425946 1106 96-97 -11.332588670595113 1106 98-99 -10.862937390105435 1106 100-101 -10.698534700706858 1107 1 0.07625725116542625 1107 2 0.07913282928707943 1107 3 -0.020908295423428314 1107 4 0.08470156433509857 1107 5 0.05688901105079225 1107 6 0.09041490067325242 1107 7 0.10073257839552241 1107 8 -0.10101590736259425 1107 9 -0.025866564978990425 1107 10-11 0.21364984003381693 1107 12-13 0.22489289252238365 1107 14-15 0.22382059627040718 1107 16-17 0.03940263441711522 1107 18-19 0.2270879936521979 1107 20-21 0.1047221481900209 1107 22-23 0.13495734854948438 1107 24-25 0.13897945397244627 1107 26-27 0.10150133158980879 1107 28-29 0.22891524556325038 1107 30-31 0.22438163809577105 1107 32-33 0.1924823544118368 1107 34-35 0.002547110106497996 1107 36-37 -0.0029356637676798414 1107 38-39 0.2691015376019692 1107 40-41 0.1403510646173416 1107 42-43 0.07575316454895642 1107 44-45 -0.1309979358318003 1107 46-47 -0.14005583201207372 1107 48-49 -0.025858864655013747 1107 50-51 -0.299322676714759 1107 52-53 -0.23387282170753565 1107 54-55 -0.2669107159965449 1107 56-57 0.15224916228121543 1107 58-59 -6.7442382493895 1107 60-61 -8.620930389336316 1107 62-63 -2.905010460170729 1107 64-65 -0.9197365804937618 1107 66-67 -2.1062522927520924 1107 68-69 -4.293638490175912 1107 70-71 -4.640632468266384 1107 72-73 -6.461643318900915 1107 74-75 -6.456438006307732 1107 76-77 -9.159987592057902 1107 78-79 -12.176884952313372 1107 80-81 -8.438469658269817 1107 82-83 -4.947291641573116 1107 84-85 -3.9820717450068166 1107 86-87 -3.692211859558906 1107 88-89 -3.7587871972105162 1107 90-91 -8.447449389976583 1107 92-93 -11.299782810718037 1107 94-95 -11.389808905625472 1107 96-97 -11.955790330562696 1107 98-99 -11.235418961354073 1107 100-101 -10.728254041737866 1108 1 0.023654287243694228 1108 2 -0.08856190444633683 1108 3 -0.09922607043634457 1108 4 -0.06460816206294595 1108 5 -0.18751313789992707 1108 6 0.005077771889844485 1108 7 -0.02998915413827774 1108 8 -0.06712193319680893 1108 9 -0.03304830797257097 1108 10-11 0.02979220443682351 1108 12-13 0.020390730914861876 1108 14-15 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8.122807320500646 2316 100-101 7.077217238218061 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 16.0 5 72.0 6 180.0 7 423.0 8 895.0 9 1644.0 10 2957.0 11 4702.0 12 6611.0 13 8802.0 14 10811.0 15 15012.0 16 19573.0 17 24866.0 18 23162.0 19 19565.0 20 19557.0 21 19435.0 22 18870.0 23 18236.0 24 18633.0 25 19587.0 26 24252.0 27 33923.0 28 46771.0 29 60947.0 30 72790.0 31 78795.0 32 80563.0 33 83552.0 34 86736.0 35 86150.0 36 75145.0 37 51103.0 38 18699.0 39 1034.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.07143440032945 24.232299864688166 16.242733868381247 20.45353186660113 2 14.180931878966357 24.454920727385886 41.475833972947406 19.888313420700346 3 15.922146797781858 31.18900226273321 33.57137096965471 19.317479969830224 4 10.35580959609136 21.10020634205346 41.96655835685317 26.577425705002018 5 9.679697801963073 41.316695606109654 36.84837598934446 12.15523060258281 6 23.775442923890616 43.583236486967245 19.38230201835733 13.25901857078481 7 22.46415103289614 37.05495339515689 25.005146692597773 15.475748879349194 8 23.43936330785439 36.93150284657704 22.320560340697465 17.308573504871106 9 25.64238787562555 16.932001992268102 23.86148993193084 33.56412020017551 10-11 21.304698432274744 29.901050684249224 30.222564808007018 18.571686075469014 12-13 23.212152835424423 28.762422028793015 30.113464411925143 17.911960723857412 14-15 20.842844050797193 29.564527727654795 29.315161933602386 20.27746628794563 16-17 17.239617674264167 32.671109740767974 30.655740815406872 19.43353176956099 18-19 17.194103935358456 30.457782723737477 34.70806662625147 17.640046714652595 20-21 18.23020183573275 29.75139340179778 34.765505300856205 17.252899461613264 22-23 18.265493442117496 29.68223323767284 34.31458862035434 17.737684699855325 24-25 17.89976994046913 30.532030453240992 34.450015416360316 17.118184189929558 26-27 17.34064782071442 30.34881376755394 34.96287020105353 17.34766821067811 28-29 17.079484316313064 29.825379462400992 35.46800599967554 17.627130221610408 30-31 17.788049869614326 30.157492230702708 34.84497221743881 17.20948568224416 32-33 17.278144793782964 30.60276271581197 34.80356882811081 17.315523662294254 34-35 17.618310109694633 30.399288467239842 34.77445597391047 17.207945449155055 36-37 17.819249634895197 29.86936886925215 34.27496685825773 18.03641463759493 38-39 16.95520643150771 30.75638728729603 34.49249012157766 17.795916159618606 40-41 17.103629952134586 30.192458065569166 35.150238947492284 17.553673034803964 42-43 17.12286258987822 30.06741400301959 34.424508937764465 18.385214469337733 44-45 17.03549585541643 30.232827379399023 34.186669956395285 18.545006808789267 46-47 17.638303162353903 30.311822671317895 33.46027413899781 18.58960002733039 48-49 17.28133448855392 30.50793012117479 33.19192003545376 19.018815354817526 50-51 17.061237194013305 30.47393063605756 32.94796755373864 19.51686461619049 52-53 17.159233837469202 30.85445312637146 32.81293960854942 19.173373427609917 54-55 17.372836727548414 30.52929070187807 32.542383013205225 19.55548955736829 56-57 17.621498661654723 30.63960266775875 31.79085516320021 19.948043507386316 58-59 16.505279881010303 30.124763296725774 33.12646192052505 20.243494901738867 60-61 17.980131835837593 30.873406476067938 31.211918043465953 19.93454364462852 62-63 16.857466771656533 30.52596127391919 32.32441868187122 20.29215327255306 64-65 16.57492837002865 30.103302958678817 32.44424452230219 20.87752414899034 66-67 18.22793030051589 30.16559038926368 30.23947472852556 21.367004581694864 68-69 19.956051860558958 29.624593803789928 29.006844174654134 21.412510160996977 70-71 19.027441299201357 29.80150013017418 29.855121226827418 21.315937343797042 72-73 18.620039907815844 29.702720592143088 29.869550723027196 21.80768877701387 74-75 18.724226704795086 30.05556433399878 29.30900591196035 21.91120304924578 76-77 16.782925619533422 30.379562856104002 30.79990828478151 22.037603239581067 78-79 17.191723888257723 30.250060139237224 30.057805120290382 22.50041085221467 80-81 17.29720550398834 30.450149515403126 29.541957285650316 22.71068769495822 82-83 17.698598297085123 30.178640039845362 29.467068282617458 22.655693380452053 84-85 17.459763299575457 29.881175438180396 29.26670303346536 23.392358228778786 86-87 17.50185949785968 30.141226964995905 29.425557469868547 22.931356067275875 88-89 18.055697201668384 29.59126703093567 29.173175140846308 23.17986062654964 90-91 18.015865394215016 29.43550725808982 29.068110139266707 23.480517208428456 92-93 17.967030799986127 29.41183179064924 29.168015000601105 23.45312240876353 94-95 17.62994088616936 29.022613538527764 29.131145839739254 24.216299735563616 96-97 17.58132868513362 28.91900057301966 29.285099135283073 24.214571606563645 98-99 17.59664526525503 28.91063573353925 28.996813021153432 24.49590598005228 100-101 17.023755095820476 28.882157960909815 29.5320417571532 24.562045186116507 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1463.0 1 1050.5 2 610.0 3 590.0 4 625.5 5 734.0 6 963.0 7 1425.5 8 1848.0 9 2040.0 10 2086.5 11 2124.0 12 2328.5 13 2575.5 14 2767.5 15 2899.5 16 3109.5 17 3406.5 18 3610.5 19 3925.0 20 4456.0 21 4941.0 22 5527.0 23 6317.0 24 7260.0 25 8664.5 26 10636.5 27 12815.0 28 14920.5 29 17502.5 30 20834.5 31 24599.5 32 29760.5 33 36071.0 34 41934.0 35 47496.5 36 52697.5 37 55513.0 38 55353.5 39 53717.0 40 51185.0 41 48395.5 42 46064.0 43 44283.0 44 42575.5 45 40274.0 46 37347.0 47 33955.0 48 30416.0 49 26479.5 50 22363.5 51 18617.0 52 14921.0 53 11666.5 54 9023.5 55 6836.0 56 5179.5 57 3840.5 58 2841.5 59 2125.5 60 1555.5 61 1157.5 62 820.5 63 523.0 64 326.5 65 200.5 66 134.5 67 95.0 68 87.5 69 78.0 70 60.0 71 48.5 72 34.5 73 26.0 74 20.5 75 13.0 76 11.0 77 10.0 78 6.5 79 4.0 80 3.0 81 2.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02020729075255556 2 0.005122975120366198 3 0.003794796385456443 4 0.0 5 0.06887555439603445 6 0.0 7 0.0 8 1.8973981927282213E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 9.486990963641107E-5 16-17 0.0 18-19 0.001517918554182577 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.3717477409102768E-4 28-29 0.0017076583734553993 30-31 0.008206247183549557 32-33 3.3204468372743874E-4 34-35 0.0011858738704551384 36-37 0.025425135782558166 38-39 0.02495078623437611 40-41 0.027227664065649977 42-43 0.02732253397528639 44-45 0.02751227379455921 46-47 0.028745582619832555 48-49 0.02921993216801461 50-51 0.0257097455114674 52-53 0.021393164623010698 54-55 0.016507364276735527 56-57 0.15710457035789674 58-59 0.7526978630552854 60-61 0.3129283969357019 62-63 4.7434954818205535E-4 64-65 0.13689727960534118 66-67 1.3862865545620568 68-69 2.6651803714156963 70-71 2.1596660579180798 72-73 1.8205535659227285 74-75 1.5349002680074946 76-77 1.111685601119465 78-79 0.42003652491521004 80-81 2.846097289092332E-4 82-83 0.0 84-85 0.0 86-87 0.002561487560183099 88-89 4.743495481820553E-5 90-91 0.22313402746483882 92-93 0.17555676778217869 94-95 1.2685055617484524 96-97 1.2426535113725303 98-99 0.9768280245713066 100-101 0.3979792709247445 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1054075.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.58490443570562 #Duplication Level Percentage of deduplicated Percentage of total 1 74.41821509024119 24.993286420815362 2 11.8310408621567 7.946887534609219 3 4.648172450849753 4.683252826874055 4 2.392315861665775 3.213827983762712 5 1.3554753701038846 2.276175538494584 6 0.9070378405663209 1.8277667516993206 7 0.6260092992434991 1.471712374366917 8 0.48190011290423296 1.294765539155553 9 0.3401995833661605 1.0283013446777431 >10 2.3032948759904 15.616296835854182 >50 0.36803690204094003 8.833104228174598 >100 0.3085754654113708 19.483621662795567 >500 0.016626440601807305 3.662613848636224 >1k 0.0022544326239738715 1.268035358640485 >5k 5.636081559934679E-4 1.4055525534534887 >10k+ 2.8180407799673394E-4 0.9947991979900088 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10511 0.9971776201883168 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8598 0.8156914830538623 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6253 0.5932215449564784 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3423 0.32473970068543506 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1770 0.1679197400564476 No Hit CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT 1730 0.16412494367099115 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1630 0.15463795270735003 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1568 0.14875601830989255 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1204 0.11422337120223892 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.743495481820553E-5 0.0 12-13 0.0 0.0 0.0 9.486990963641107E-5 0.0 14-15 0.0 0.0 0.0 9.486990963641107E-5 0.0 16-17 0.0 0.0 0.0 1.8973981927282213E-4 0.0 18-19 0.0 0.0 0.0 2.3717477409102768E-4 0.0 20-21 0.0 0.0 0.0 7.589592770912885E-4 0.0 22-23 0.0 0.0 0.0 0.0024191826957284824 0.0 24-25 0.0 0.0 0.0 0.006213979081184925 0.0 26-27 0.0 0.0 0.0 0.014609966084007305 0.0 28-29 0.0 0.0 0.0 0.04193250005929369 0.0 30-31 0.0 0.0 0.0 0.12456419135260774 0.0 32-33 0.0 0.0 0.0 0.2980812560776036 0.0 34-35 0.0 0.0 0.0 0.5647605720655551 0.0 36-37 0.0 0.0 0.0 0.9564309939994782 0.0 38-39 0.0 0.0 0.0 1.512748144107393 0.0 40-41 0.0 0.0 0.0 2.1238052320755165 0.0 42-43 0.0 0.0 0.0 2.694400303583711 0.0 44-45 0.0 0.0 0.0 3.3495244645779474 0.0 46-47 0.0 0.0 0.0 4.091881507482864 0.0 48-49 0.0 0.0 0.0 4.7756563811872965 0.0 50-51 0.0 0.0 0.0 5.438749614590993 0.0 52-53 0.0 0.0 0.0 6.046391385812205 0.0 54-55 0.0 0.0 0.0 6.677703199487702 0.0 56-57 0.0 0.0 0.0 7.224723098451248 0.0 58-59 0.0 9.486990963641107E-5 0.0 7.599506676469891 0.0 60-61 0.0 9.486990963641107E-5 0.0 7.8645732039940235 0.0 62-63 0.0 9.486990963641107E-5 0.0 8.083865000118587 0.0 64-65 0.0 9.486990963641107E-5 0.0 8.305291369209971 0.0 66-67 0.0 9.486990963641107E-5 0.0 8.485307022745062 0.0 68-69 0.0 9.486990963641107E-5 0.0 8.689277328463344 0.0 70-71 0.0 9.486990963641107E-5 0.0 9.047933021843797 0.0 72-73 0.0 9.486990963641107E-5 0.0 9.48400256148756 0.0 74-75 0.0 9.486990963641107E-5 0.0 10.000237174774092 0.0 76-77 0.0 9.486990963641107E-5 0.0 10.710480753267081 0.0 78-79 0.0 9.486990963641107E-5 0.0 11.70789554822949 0.0 80-81 0.0 9.486990963641107E-5 0.0 12.777269169651117 0.0 82-83 0.0 9.486990963641107E-5 0.0 13.753053625216422 0.0 84-85 0.0 9.486990963641107E-5 0.0 14.526717738301354 0.0 86-87 0.0 9.486990963641107E-5 0.0 15.19379550790978 0.0 88-89 0.0 9.486990963641107E-5 0.0 15.851813201147927 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5070 0.0 43.616318 1 GTATCAA 8760 0.0 36.654484 1 CTGGTCG 305 0.0 29.347216 9 TCAACGC 10860 0.0 29.34116 4 ATCAACG 10885 0.0 29.273771 3 CAACGCA 10970 0.0 29.003979 5 AACGCAG 10940 0.0 28.980837 6 TATCAAC 11035 0.0 28.877222 2 AGGGCGC 35 0.007538012 27.747246 62-63 TTACCGA 45 7.738116E-4 27.054535 64-65 GTACATG 6205 0.0 27.05156 1 GCCGGCG 45 8.416478E-4 26.668116 88-89 ACGCAGA 12260 0.0 25.783697 7 CGCAGAG 12205 0.0 25.706894 8 CGGTCGC 95 9.149517E-9 25.629984 68-69 CATGGGG 2105 0.0 25.079931 4 ACATGGG 6285 0.0 25.049648 3 CGCAGAA 170 1.2158489E-7 24.94059 9 TACATGG 6620 0.0 24.708849 2 GGTCGCG 50 0.0014309558 24.348484 68-69 >>END_MODULE