FastQCFastQC Report
Fri 27 May 2016
SRR939800_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939800_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1091665
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100040.9163983456463293No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77330.7083674936908301No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53830.49309998946563277No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31410.2877256301154658No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15030.13767959951083894No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14880.13630555161152919No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14430.13218340791359987No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11690.10708413295287472No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACGT150.005137934356.6715294-95
GGTATCA55000.041.8266831
GTATCAA92150.035.430021
CATTCCG2250.031.368649
CGACGTA400.006803756628.3357694-95
GGGAGCG1005.2825864E-528.2317777
TATCAAC116250.028.006592
GTACATG66100.027.7568651
ATCAACG116700.027.6176513
TCAACGC117600.027.44634
CAACGCA118300.027.2838975
AACGCAG118500.027.2788036
ACATGGG68250.025.576333
TACATGG71000.025.4460262
CATGGGG23450.024.67914
GTACACG1151.368362E-424.5448671
ACGCAGA130600.024.2470467
CGCAGAG130100.024.0870658
CGCAGGG1401.9144456E-523.5264828
GCAGAGT128800.023.1246289