FastQCFastQC Report
Fri 27 May 2016
SRR939800_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939800_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1091665
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105360.9651312444751824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89090.816092848996716No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62770.574993244264495No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36250.3320615756665277No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT19670.18018348119615452No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17440.1597559690930826No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17280.1582903180004855No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17220.15774069884076158No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12070.11056505429779281No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGGG555.004736E-543.1207968
GGTATCA59100.042.7935521
CGCAGAA1450.042.5260289
GTATCAA94950.033.782391
CGGGTCG600.003978308831.6219186
ATGGGCG902.4362864E-531.6219165
CCGGGTC650.005884465829.1894635
GTCGGGA650.005884465829.1894639
CATTCCG2400.027.6691789
CGGATTA1257.472074E-626.5624089
ATCAACG120200.026.4787543
TCAACGC120550.026.4018734
GTACATG61950.026.348491
TATCAAC121950.026.1813662
CAACGCA121650.026.1241515
CATGGGT8600.025.9226194
AACGCAG122800.025.87956
CTGCACG950.001236667324.9646724
ACATGGG62800.024.9249213
TACATGG66500.024.2558382
Produced by FastQC (version 0.11.5)