##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939800_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1091665 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.351966949567863 31.0 31.0 34.0 30.0 34.0 2 31.562593836021122 31.0 31.0 34.0 30.0 34.0 3 31.564574297059995 31.0 31.0 34.0 30.0 34.0 4 35.421567971859496 37.0 35.0 37.0 33.0 37.0 5 35.176278437066316 37.0 35.0 37.0 32.0 37.0 6 35.20839543266478 37.0 35.0 37.0 32.0 37.0 7 35.117758653066645 37.0 35.0 37.0 32.0 37.0 8 35.08782364553228 37.0 35.0 37.0 32.0 37.0 9 36.61845346328773 39.0 35.0 39.0 32.0 39.0 10-11 36.493971135833796 38.0 35.0 39.0 32.0 39.0 12-13 36.43478402257102 38.0 35.0 39.0 32.0 39.0 14-15 37.33802311148567 39.0 36.0 41.0 32.0 41.0 16-17 37.28292012659561 39.0 36.0 40.5 32.0 41.0 18-19 37.39649113968113 39.0 36.0 41.0 32.0 41.0 20-21 37.381652338400514 39.0 36.0 41.0 32.0 41.0 22-23 37.23745517168729 39.0 36.0 40.0 31.0 41.0 24-25 37.0898554043594 39.0 36.0 40.0 31.0 41.0 26-27 36.77741340063115 39.0 35.5 40.0 30.5 41.0 28-29 36.55594527625233 38.0 35.0 40.0 30.0 41.0 30-31 36.2077633706311 38.0 35.0 40.0 30.0 41.0 32-33 36.01128093325333 38.0 35.0 40.0 30.0 41.0 34-35 35.78838792120293 38.0 34.0 40.0 29.5 41.0 36-37 35.65204893442585 38.0 34.5 40.0 29.0 41.0 38-39 35.476059963450325 38.0 34.0 40.0 27.0 41.0 40-41 35.34586663491089 38.0 34.0 40.0 27.0 41.0 42-43 35.01497254194281 38.0 34.0 40.0 26.5 41.0 44-45 34.60258687417843 38.0 33.5 40.0 24.5 41.0 46-47 34.09186288834029 38.0 33.0 40.0 23.0 41.0 48-49 33.63542616095597 38.0 33.0 40.0 18.5 41.0 50-51 33.3644336861583 38.0 33.0 40.0 15.0 41.0 52-53 33.14775961490018 38.0 33.0 40.0 13.5 41.0 54-55 32.9048403127333 38.0 32.5 40.0 9.0 41.0 56-57 32.5550136717766 37.0 31.5 40.0 8.0 41.0 58-59 32.2347652439164 37.0 31.0 40.0 6.5 41.0 60-61 31.93772952325118 37.0 31.0 40.0 2.0 41.0 62-63 31.649668167432317 36.0 31.0 39.0 2.0 41.0 64-65 31.242720065221473 36.0 30.0 39.0 2.0 40.0 66-67 30.383512341240216 35.5 28.5 39.0 2.0 40.0 68-69 28.56423811334063 34.0 24.5 38.0 2.0 40.0 70-71 27.935025855001307 34.0 23.5 37.0 2.0 39.5 72-73 28.412774065303914 34.0 26.0 37.0 2.0 39.0 74-75 28.431958980090045 34.0 26.0 36.5 2.0 39.0 76-77 27.852807408866273 32.5 26.0 35.5 2.0 38.5 78-79 28.27767492774798 34.0 26.0 36.0 2.0 38.5 80-81 28.0094186403338 34.0 26.0 36.0 2.0 37.5 82-83 27.510600779543175 33.5 26.0 35.0 2.0 37.0 84-85 27.0992712965974 33.0 26.0 35.0 2.0 36.5 86-87 26.688441509071005 33.0 25.0 35.0 2.0 36.0 88-89 26.191905941841135 32.5 23.5 35.0 2.0 36.0 90-91 25.6698020913009 32.0 22.0 35.0 2.0 35.0 92-93 25.165574603930693 32.0 19.5 34.5 2.0 35.0 94-95 24.373093394035713 31.0 12.0 34.0 2.0 35.0 96-97 23.84816633307837 31.0 4.5 34.0 2.0 35.0 98-99 23.212356354742525 31.0 2.0 34.0 2.0 35.0 100-101 21.822467057201614 30.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.17782045830306714 1101 2 0.22804428390202247 1101 3 0.22328289667782286 1101 4 0.17724096018607582 1101 5 0.16832028439001334 1101 6 0.18691263160160076 1101 7 0.03316591640365374 1101 8 0.1323821469026214 1101 9 0.03611940953064163 1101 10-11 0.18323878706934948 1101 12-13 0.25094068235615197 1101 14-15 0.2983452085790006 1101 16-17 0.2455832223257559 1101 18-19 0.13850001614100194 1101 20-21 0.09018520990767342 1101 22-23 0.25489992497738 1101 24-25 0.11928827534809727 1101 26-27 0.015640273457186993 1101 28-29 0.06252824898108855 1101 30-31 -0.09419935218038944 1101 32-33 0.011427676963556621 1101 34-35 0.02044483329235902 1101 36-37 0.10878621720704018 1101 38-39 -0.017399098479700115 1101 40-41 0.09973301812656388 1101 42-43 -0.015804105962452297 1101 44-45 0.19948865985710285 1101 46-47 0.01413738101052786 1101 48-49 0.09284206703114961 1101 50-51 0.12226526870419008 1101 52-53 -0.219787979269654 1101 54-55 -0.3440700656713105 1101 56-57 -0.45617749085412385 1101 58-59 -0.4225360801670668 1101 60-61 -0.5057606658916498 1101 62-63 -0.5059332234287162 1101 64-65 -0.35773962753121324 1101 66-67 -0.42429726905811194 1101 68-69 -8.694193723386935 1101 70-71 -6.259795048653441 1101 72-73 -1.7722325843634152 1101 74-75 -0.8444443204893979 1101 76-77 -0.7516890790605046 1101 78-79 -0.7067229571659688 1101 80-81 -0.7525325179204501 1101 82-83 -0.6104653998150802 1101 84-85 -0.6747887346188506 1101 86-87 -0.7296156161199647 1101 88-89 -0.9195051836706476 1101 90-91 -1.1599985105385677 1101 92-93 -1.3209264015359459 1101 94-95 -1.1539993935310164 1101 96-97 -1.6263301635810912 1101 98-99 -1.6425742333216355 1101 100-101 -1.4385637552315238 1102 1 0.14059916946375495 1102 2 0.09080922105171751 1102 3 0.09804992883758601 1102 4 -0.004799600025059192 1102 5 0.16229202896203532 1102 6 0.1895194907189719 1102 7 0.23681551916797616 1102 8 0.15328463370678236 1102 9 0.1709644263995571 1102 10-11 0.37508270201738014 1102 12-13 0.1718761587668851 1102 14-15 0.2013204799780297 1102 16-17 0.3171141271252367 1102 18-19 0.27760859956049444 1102 20-21 0.17288666757215054 1102 22-23 0.39966733124608567 1102 24-25 0.2669460983061498 1102 26-27 0.4347719496012843 1102 28-29 -0.0457966952426645 1102 30-31 0.12574141957235696 1102 32-33 -0.053157555256063915 1102 34-35 -0.07601873189783248 1102 36-37 0.3019420641896815 1102 38-39 0.3573088414724097 1102 40-41 0.47819480119779456 1102 42-43 0.45962203813071056 1102 44-45 0.26950782443783083 1102 46-47 0.30762414435601215 1102 48-49 0.36490089056056263 1102 50-51 0.21888442920372597 1102 52-53 0.12349736042126125 1102 54-55 -0.12296785570776336 1102 56-57 -0.26586869793362666 1102 58-59 -0.11700343406051417 1102 60-61 -0.13374460981611946 1102 62-63 -0.1418436005305317 1102 64-65 0.10410466152576348 1102 66-67 0.19799469236105338 1102 68-69 -4.4778439723394285 1102 70-71 0.24446639227815936 1102 72-73 0.4750334445041915 1102 74-75 0.27581342356566907 1102 76-77 -0.05910034722179702 1102 78-79 0.04448269391962967 1102 80-81 -0.07537492963074044 1102 82-83 -0.011179862866722345 1102 84-85 -0.22517100593625727 1102 86-87 -0.4472360621938627 1102 88-89 -0.4167707874144604 1102 90-91 -0.5584653440804992 1102 92-93 -0.5608573444265126 1102 94-95 -0.09495182387873413 1102 96-97 -0.5117605759171653 1102 98-99 -0.48536557916154166 1102 100-101 -0.3721789739832282 1103 1 0.17329075663745286 1103 2 0.1262561224008607 1103 3 0.18879127744948576 1103 4 0.06357015584283232 1103 5 0.3278777901880332 1103 6 0.11856849287083548 1103 7 0.1204590325748498 1103 8 0.0836524399660874 1103 9 0.05218584954751293 1103 10-11 0.14288655746783263 1103 12-13 0.19034480421995426 1103 14-15 0.24819729323005646 1103 16-17 0.3783792506833379 1103 18-19 0.2101796624618899 1103 20-21 0.31993332555178 1103 22-23 -0.03405550934688506 1103 24-25 0.006409757005286565 1103 26-27 -0.05902403447028348 1103 28-29 -0.04464284526305562 1103 30-31 -0.01939997678505989 1103 32-33 -0.09854853798479013 1103 34-35 -0.13223576179514396 1103 36-37 -0.07419396411280132 1103 38-39 0.07692246751228282 1103 40-41 0.07422338551007357 1103 42-43 0.16991036799918646 1103 44-45 0.049611538309562775 1103 46-47 0.021272896507049666 1103 48-49 -0.13644319546094152 1103 50-51 -0.14590789437824725 1103 52-53 -0.2133953348462967 1103 54-55 -0.24688902255513767 1103 56-57 0.06004542976573646 1103 58-59 -0.13501203156913277 1103 60-61 -0.23927090466457557 1103 62-63 0.054260746632426304 1103 64-65 0.07504289407958353 1103 66-67 0.1520553822174513 1103 68-69 -3.329495088556662 1103 70-71 0.6811310529700059 1103 72-73 0.5313710815653216 1103 74-75 0.34238857799257616 1103 76-77 0.15713379148556328 1103 78-79 -0.006197182339906959 1103 80-81 0.316885571896119 1103 82-83 0.12267916849611282 1103 84-85 0.14676408005540864 1103 86-87 -0.11448048276554701 1103 88-89 -0.288074654469721 1103 90-91 -0.30697827018436286 1103 92-93 -0.22060256904596187 1103 94-95 -0.5109184716461321 1103 96-97 -0.5133363437413259 1103 98-99 -0.15670525535410107 1103 100-101 -0.3579614202114989 1104 1 0.3361007603341939 1104 2 0.25783029023280335 1104 3 0.2379112928793532 1104 4 0.27297674889617696 1104 5 0.32475163495714554 1104 6 0.23994448335574958 1104 7 0.23817284069803435 1104 8 0.2084768448714982 1104 9 0.37747475772373207 1104 10-11 0.41522490550775615 1104 12-13 0.31918619805122006 1104 14-15 0.46937157035689125 1104 16-17 0.41934715334659245 1104 18-19 0.38204105082841977 1104 20-21 0.4219203226747581 1104 22-23 0.3159087287230946 1104 24-25 0.5289373620007041 1104 26-27 0.5348976428847791 1104 28-29 0.5598607470027233 1104 30-31 0.49062943639783185 1104 32-33 0.39061398994166296 1104 34-35 0.3741901346640262 1104 36-37 0.34336152715165014 1104 38-39 0.30609857693576004 1104 40-41 0.20778374682611656 1104 42-43 0.14047227230186365 1104 44-45 0.06634972883625778 1104 46-47 0.0698070591957034 1104 48-49 -0.044964580291456 1104 50-51 0.060122611753520516 1104 52-53 9.078298836016074E-4 1104 54-55 0.03263586614087188 1104 56-57 0.21127379178897598 1104 58-59 0.03683850767255592 1104 60-61 0.17347922793583237 1104 62-63 0.275081020834012 1104 64-65 0.09506636033478699 1104 66-67 0.42517928725948195 1104 68-69 -3.99029880779738 1104 70-71 0.18615259049190414 1104 72-73 0.7097720812920834 1104 74-75 0.3722164385451556 1104 76-77 0.2764227318073367 1104 78-79 0.05010736464885568 1104 80-81 -0.2679967753723105 1104 82-83 -0.3058974263252381 1104 84-85 0.014257691126335459 1104 86-87 0.17124126530973527 1104 88-89 0.1122938139553078 1104 90-91 -0.029947072923164342 1104 92-93 0.11614783679167218 1104 94-95 0.39886141166550004 1104 96-97 -0.09281863957687264 1104 98-99 0.39990507622729865 1104 100-101 0.43983821654373045 1105 1 0.010119182766672452 1105 2 0.13397347495016732 1105 3 0.11718175203148107 1105 4 0.1397913271915172 1105 5 0.2690382548242667 1105 6 0.16479285437133484 1105 7 0.1426868844767455 1105 8 0.06325423395733765 1105 9 0.18489616499722672 1105 10-11 0.14444175647700774 1105 12-13 0.23245246246086282 1105 14-15 0.23093763363802822 1105 16-17 0.2484697008700536 1105 18-19 0.14974035979155076 1105 20-21 0.17074915996413864 1105 22-23 0.18164801295603183 1105 24-25 0.15470959374457038 1105 26-27 0.28898634290591474 1105 28-29 0.12669645553072684 1105 30-31 0.03035231730940069 1105 32-33 -0.011276774581453708 1105 34-35 0.1042858467919956 1105 36-37 0.3671400823132913 1105 38-39 0.5032393434153803 1105 40-41 0.1352233104476781 1105 42-43 0.15787223630221092 1105 44-45 0.09857098585032986 1105 46-47 0.1487006958652657 1105 48-49 0.2183040319218179 1105 50-51 0.03714583153141149 1105 52-53 -0.11692139088037834 1105 54-55 -0.031886864511264434 1105 56-57 -0.007222205205827947 1105 58-59 -0.2658152930302222 1105 60-61 -0.013260377163593517 1105 62-63 -0.29254394101418413 1105 64-65 -0.36543262619664674 1105 66-67 0.5037180142803521 1105 68-69 1.5176989852672094 1105 70-71 1.7387484787051513 1105 72-73 0.9166124071672286 1105 74-75 0.4700134161392846 1105 76-77 0.27169308139529846 1105 78-79 0.16318580476812272 1105 80-81 0.31061551616229366 1105 82-83 0.30364522152769524 1105 84-85 0.29889209897113034 1105 86-87 0.13299554418185267 1105 88-89 0.2509085315536588 1105 90-91 0.4240919319211365 1105 92-93 0.5330614141400645 1105 94-95 0.9741276917271726 1105 96-97 0.12294881829961923 1105 98-99 0.4679757294645306 1105 100-101 0.43663033418434694 1106 1 0.431028828082205 1106 2 0.36994374814868536 1106 3 0.44785511404059974 1106 4 0.3465943660465598 1106 5 0.5198605752275398 1106 6 0.42746177876976077 1106 7 0.4624997782011633 1106 8 0.5243294462684673 1106 9 0.5901833445842186 1106 10-11 0.4851638089368322 1106 12-13 0.5410196786386834 1106 14-15 0.753190564472284 1106 16-17 0.5797439893277243 1106 18-19 0.6251591430817385 1106 20-21 0.6355653624333684 1106 22-23 0.5350941355612377 1106 24-25 0.626048402726191 1106 26-27 0.578435826157822 1106 28-29 0.6778108861995449 1106 30-31 0.7671351793087879 1106 32-33 0.757072972721268 1106 34-35 0.6619974929249821 1106 36-37 0.719463015807527 1106 38-39 0.8276236573698483 1106 40-41 0.69066587128642 1106 42-43 0.6992420890954989 1106 44-45 0.8526071679480722 1106 46-47 0.8345848443824906 1106 48-49 0.8714064667687396 1106 50-51 0.9683677315294688 1106 52-53 1.0667358426196927 1106 54-55 1.035926995885653 1106 56-57 1.0933028147439572 1106 58-59 1.1768225875565292 1106 60-61 1.1679371938284469 1106 62-63 1.262517465397 1106 64-65 1.1466475362562356 1106 66-67 1.4976187324726098 1106 68-69 3.2538265860379525 1106 70-71 3.41715389077363 1106 72-73 2.334516609273148 1106 74-75 1.8301419057297146 1106 76-77 1.512883548317582 1106 78-79 1.2890470515810257 1106 80-81 1.1967622557510964 1106 82-83 1.3327013534103713 1106 84-85 1.3593245389433655 1106 86-87 1.3322574021393834 1106 88-89 1.1100979215119438 1106 90-91 1.054630835623687 1106 92-93 1.4718399034061065 1106 94-95 1.8140655392264726 1106 96-97 1.3320071560121711 1106 98-99 1.413760161495773 1106 100-101 1.7985105122077911 1107 1 0.19156273676155422 1107 2 0.11595959479733864 1107 3 0.1485825433299155 1107 4 0.11886626523462951 1107 5 0.25606464099177373 1107 6 0.17932613762630467 1107 7 0.18157680409494503 1107 8 0.3322715668019569 1107 9 0.3014663704844267 1107 10-11 0.2090511377226676 1107 12-13 0.11257097621959389 1107 14-15 0.2827849519569057 1107 16-17 0.3357278502936225 1107 18-19 0.3634697496048247 1107 20-21 0.38576256195912606 1107 22-23 0.2722408230911668 1107 24-25 0.5117256927307849 1107 26-27 0.45642153140116193 1107 28-29 0.3781041552295008 1107 30-31 0.5674672456385181 1107 32-33 0.5202371455435539 1107 34-35 0.5646010086190074 1107 36-37 0.4439138291852842 1107 38-39 0.516606268468287 1107 40-41 0.5200212095699541 1107 42-43 0.6217362494191221 1107 44-45 0.46345217795665405 1107 46-47 0.6031750163758218 1107 48-49 0.478124745357448 1107 50-51 0.3556611740628739 1107 52-53 0.4142026118890172 1107 54-55 0.2652282749916637 1107 56-57 0.2679930789511573 1107 58-59 0.4123973016774656 1107 60-61 0.4635187998920749 1107 62-63 0.33533349068994767 1107 64-65 0.37031276455786255 1107 66-67 0.9010965931107826 1107 68-69 2.3076198855916594 1107 70-71 2.261459844833773 1107 72-73 1.3048354700623328 1107 74-75 0.6781010713708895 1107 76-77 0.3755487380628857 1107 78-79 0.29461321905041515 1107 80-81 0.3919905895074791 1107 82-83 0.4337241401971177 1107 84-85 0.39009127961675105 1107 86-87 0.6039553743981081 1107 88-89 0.806701029151558 1107 90-91 0.7159420558349119 1107 92-93 0.47339500842388915 1107 94-95 0.8844252054572372 1107 96-97 0.665171136052848 1107 98-99 0.886992836051494 1107 100-101 1.161995023811638 1108 1 -0.14774215310513483 1108 2 -0.04596418312115347 1108 3 -0.07913466125444302 1108 4 -0.050449547835313524 1108 5 -0.11159281721425884 1108 6 0.025872079016927785 1108 7 -0.0600270425981293 1108 8 -0.03584423455905039 1108 9 -0.27418027852640137 1108 10-11 -0.06294548209285722 1108 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-0.8737580019641804 2316 90-91 -1.0337296106080238 2316 92-93 -1.0610652959901827 2316 94-95 -0.8432812796505864 2316 96-97 -0.9010215370650911 2316 98-99 -0.9175519440925903 2316 100-101 -0.6361640317181418 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 21.0 5 65.0 6 190.0 7 445.0 8 901.0 9 1810.0 10 3008.0 11 4892.0 12 6884.0 13 8735.0 14 10716.0 15 13592.0 16 16907.0 17 20384.0 18 16544.0 19 11567.0 20 10607.0 21 10422.0 22 10993.0 23 12052.0 24 13661.0 25 15967.0 26 18761.0 27 22744.0 28 27604.0 29 33071.0 30 39493.0 31 46202.0 32 55043.0 33 65659.0 34 81213.0 35 101767.0 36 126597.0 37 158002.0 38 113367.0 39 11777.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.06138904159795 24.321971779365953 16.215319772768922 20.40131940626718 2 14.253239851641183 24.410669520472634 41.34063521616114 19.99545541172504 3 15.945734268296594 31.2224904160159 33.45669230029359 19.37508301539392 4 10.37351202062904 21.231146917781555 41.89581968827433 26.499521373315073 5 9.707556805430237 41.32155926955613 36.79617831477605 12.174705610237572 6 23.86989036919785 43.459892421111256 19.38297864544402 13.287238564246875 7 22.5678207142301 37.108636807079094 24.920099114655137 15.40344336403567 8 23.38913494524419 36.94998007630546 22.358141004795424 17.30274397365492 9 25.53860387573111 16.935415168572774 23.955975505306114 33.57000545039 10-11 21.350460077038285 29.828656226956074 30.26986300742444 18.551020688581204 12-13 23.139562045132894 28.69969267128652 30.266656895659384 17.894088387921204 14-15 20.83743639303266 29.504380922718966 29.312838645555182 20.345344038693188 16-17 17.326058798419293 32.648658650754534 30.68765790143836 19.337624649387813 18-19 17.21420948734273 30.453206798789008 34.673274310342464 17.659309403525807 20-21 18.252256873674614 29.62268644684954 34.82496919842626 17.30008748104959 22-23 18.272134766617963 29.651907865508193 34.331731804170694 17.74422556370315 24-25 17.838226780401296 30.49294471559014 34.52344311070073 17.14538539330783 26-27 17.350273894802413 30.30962021142114 34.99903816206511 17.341067731711338 28-29 17.056568203875823 29.778837728990915 35.521032331819896 17.64356173531337 30-31 17.832566916439184 30.186962973819686 34.79563233973948 17.18483777000165 32-33 17.295268368536405 30.595547535021765 34.71795940520821 17.391224691233624 34-35 17.687109727649272 30.342319779081745 34.748501203022464 17.222069290246516 36-37 17.915573185738626 29.858264698492693 34.19376757134447 18.03239454442421 38-39 16.965482589090055 30.79770739695481 34.41453534536666 17.822274668588477 40-41 17.129659134413693 30.189379958873303 35.11477191188708 17.56618899482592 42-43 17.165014321142976 30.057940222123285 34.36826726320138 18.408778193532356 44-45 17.055836213651034 30.236756842502437 34.154997540325596 18.552409403520933 46-47 17.652255396089124 30.310429275726147 33.47642829832825 18.56088702985648 48-49 17.405504181705574 30.479941429073754 33.07538164395607 19.039172745264608 50-51 17.07477863022365 30.428115279614726 32.861520970244875 19.635585119916747 52-53 17.15126325429266 30.8430953924059 32.839844784864965 19.165796568436473 54-55 17.400155360985146 30.547727673732766 32.47351645153677 19.57860051374532 56-57 17.381356114694242 30.656422629310047 31.997252821516327 19.96496843447938 58-59 16.99031260936564 30.22770040365654 32.48267098601769 20.299316000960125 60-61 17.494314316927877 30.93739908341979 31.6082295252426 19.960057074409733 62-63 16.961703191835802 30.52765804252208 32.22097486865 20.289663896992106 64-65 16.986752130684096 30.161884813204722 31.892860954398074 20.958502101713112 66-67 16.9711857137287 30.40552845304691 31.23470466675382 21.388581166470566 68-69 17.103185078962397 30.16907308287718 31.207095900062825 21.520645938097598 70-71 17.437532591391548 30.168293438864296 31.020474981307373 21.373698988436782 72-73 17.405574559562073 29.920779676648934 30.736705416485993 21.936940347303 74-75 17.243687422859516 30.56847784355749 30.018839000763275 22.16899573281972 76-77 17.236465931165927 30.38810797932779 30.424852472235457 21.950573617270827 78-79 17.464847697019163 30.12535646986507 29.954599045260792 22.45519678785497 80-81 17.51241019270952 30.33180261312535 29.418408765503006 22.737378428662115 82-83 17.699356212167846 30.18799740579519 29.35903354878424 22.753612833252724 84-85 17.469135378379306 29.861426952011268 29.27035599372517 23.399081675884258 86-87 17.477224947442185 30.07987871626109 29.479185273735748 22.963711062560975 88-89 18.07879354709283 29.565076401783337 29.216663017799448 23.139467033324387 90-91 17.984907347271168 29.25580104373443 29.27938903886477 23.479902570129628 92-93 18.176992228633093 29.398549241024135 28.9535747051099 23.470883825232875 94-95 17.92400333329972 28.543670718392093 29.39986853817939 24.132457410128794 96-97 18.38063442124156 28.50314663627377 29.04181741722042 24.07440152526425 98-99 18.275365744609115 28.447317017752173 28.904039682190657 24.373277555448055 100-101 18.102332347894436 28.429560968191424 29.075516622524987 24.39259006138915 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2660.0 1 1674.0 2 757.5 3 866.0 4 763.5 5 777.0 6 1268.5 7 2290.5 8 3289.5 9 3514.5 10 2959.5 11 2393.0 12 2438.5 13 2512.0 14 2646.0 15 2867.0 16 3021.0 17 3268.5 18 3515.5 19 3820.5 20 4252.0 21 4790.5 22 5334.5 23 6038.5 24 6749.0 25 8083.5 26 10859.0 27 13822.5 28 15040.5 29 16733.5 30 20550.0 31 24321.0 32 29867.0 33 36663.0 34 42757.0 35 48485.0 36 54994.0 37 59355.5 38 58033.5 39 55044.5 40 52078.0 41 48082.5 42 44338.5 43 43393.5 44 43143.0 45 41840.0 46 38859.0 47 34870.5 48 32408.5 49 29095.5 50 24954.0 51 20870.0 52 16653.0 53 13067.5 54 10154.5 55 7877.0 56 5946.0 57 4372.0 58 3142.0 59 2345.0 60 1865.5 61 1431.5 62 1020.0 63 694.5 64 417.5 65 223.5 66 131.5 67 108.0 68 90.5 69 69.0 70 62.5 71 75.5 72 68.0 73 37.0 74 39.0 75 38.5 76 21.0 77 8.5 78 6.5 79 9.5 80 5.5 81 1.5 82 1.5 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02427484622113927 2 0.023542020674840725 3 0.0 4 0.0 5 0.0 6 9.1603193287318E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.74809579861954E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 9.1603193287318E-5 26-27 4.5801596643659E-4 28-29 4.5801596643659E-5 30-31 4.5801596643659E-4 32-33 0.002061071848964655 34-35 0.0036183261348490606 36-37 0.10177114774221029 38-39 0.09407647950607557 40-41 0.10374061639788763 42-43 0.10483985471733545 44-45 0.09833602799393587 46-47 0.09288563799334044 48-49 0.09357266194299534 50-51 0.09274823320340947 52-53 0.07254972908355585 54-55 0.06022909958641158 56-57 0.03329776075994009 58-59 0.013099256640086474 60-61 0.009847343278386683 62-63 0.0018778654623900191 64-65 0.005083977227446149 66-67 0.15274832480660278 68-69 0.4123975761795056 70-71 0.39737465248038545 72-73 0.18160333069210793 74-75 0.028580196305643214 76-77 0.03160310168412471 78-79 0.005312985210664444 80-81 0.0020152702523209957 82-83 9.1603193287318E-5 84-85 2.29007983218295E-4 86-87 9.1603193287318E-4 88-89 1.83206386574636E-4 90-91 7.32825546298544E-4 92-93 0.002702294201975881 94-95 0.07822912706736956 96-97 0.016671781178291874 98-99 0.017679416304452373 100-101 0.009526732101881073 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1091665.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.45204706608241 #Duplication Level Percentage of deduplicated Percentage of total 1 74.59408988352007 24.95325005635095 2 11.62948143278958 7.780599204876169 3 4.603443152984664 4.619837910590334 4 2.3508894243797367 3.1456825468602534 5 1.4195600317904942 2.374359449829252 6 0.9101298201956798 1.8267423348858596 7 0.6638724256405385 1.554552413988111 8 0.46506861386477005 1.2445997727969595 9 0.3422629224579117 1.0304455851933276 >10 2.3220508677196823 15.553072340422666 >50 0.3681330276654901 8.77244180120947 >100 0.3116430415211278 19.83288112349414 >500 0.016100647503523982 3.5849738307982157 >1k 0.002456030975113828 1.3784498700072079 >5k 5.457846611364062E-4 1.3863006441012886 >10k+ 2.728923305682031E-4 0.9618111145957576 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10536 0.9651312444751824 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8909 0.816092848996716 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6277 0.574993244264495 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3625 0.3320615756665277 No Hit CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT 1967 0.18018348119615452 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1744 0.1597559690930826 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1728 0.1582903180004855 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1722 0.15774069884076158 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1207 0.11056505429779281 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.5801596643658996E-5 0.0 12-13 0.0 0.0 0.0 9.160319328731799E-5 0.0 14-15 0.0 0.0 0.0 9.160319328731799E-5 0.0 16-17 0.0 0.0 0.0 9.160319328731799E-5 0.0 18-19 0.0 0.0 0.0 9.160319328731799E-5 0.0 20-21 0.0 0.0 0.0 3.6641277314927197E-4 0.0 22-23 0.0 0.0 0.0 0.0021984766388956317 0.0 24-25 0.0 0.0 0.0 0.007511461849560076 0.0 26-27 0.0 0.0 0.0 0.015893154035349674 0.0 28-29 0.0 0.0 0.0 0.04108403218936212 0.0 30-31 0.0 0.0 0.0 0.12270247740836246 0.0 32-33 0.0 0.0 0.0 0.28841265406512073 0.0 34-35 0.0 0.0 0.0 0.5407336499750381 0.0 36-37 0.0 0.0 0.0 0.9329785236313337 0.0 38-39 0.0 0.0 0.0 1.5000480916764758 0.0 40-41 0.0 0.0 0.0 2.1207513293913425 0.0 42-43 0.0 0.0 0.0 2.7004621381101344 0.0 44-45 0.0 0.0 0.0 3.35707382759363 0.0 46-47 0.0 0.0 0.0 4.107166575826833 0.0 48-49 0.0 0.0 0.0 4.882358599020762 0.0 50-51 0.0 0.0 0.0 5.6897033430585395 0.0 52-53 0.0 0.0 0.0 6.424452556416117 0.0 54-55 0.0 0.0 0.0 7.182010964902236 0.0 56-57 0.0 0.0 0.0 8.026088589448229 0.0 58-59 0.0 0.0 0.0 8.877952485423641 0.0 60-61 0.0 0.0 0.0 9.718823998204577 0.0 62-63 0.0 0.0 0.0 10.497267934760206 0.0 64-65 0.0 0.0 0.0 11.294902740309528 0.0 66-67 0.0 0.0 0.0 12.113835288298151 0.0 68-69 0.0 0.0 0.0 12.978615234527076 0.0 70-71 0.0 0.0 0.0 13.979288517997738 0.0 72-73 0.0 0.0 0.0 14.94803808860777 0.0 74-75 0.0 0.0 0.0 15.921825834848603 0.0 76-77 0.0 0.0 0.0 16.942514416052543 0.0 78-79 0.0 0.0 0.0 18.055035198527023 0.0 80-81 0.0 0.0 0.0 19.198563661929256 0.0 82-83 0.0 0.0 0.0 20.277603477257216 0.0 84-85 0.0 0.0 0.0 21.30278061493224 0.0 86-87 0.0 0.0 0.0 22.35191198765189 0.0 88-89 0.0 0.0 0.0 23.40438687692653 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTCGGG 55 5.004736E-5 43.120796 8 GGTATCA 5910 0.0 42.793552 1 CGCAGAA 145 0.0 42.526028 9 GTATCAA 9495 0.0 33.78239 1 CGGGTCG 60 0.0039783088 31.621918 6 ATGGGCG 90 2.4362864E-5 31.621916 5 CCGGGTC 65 0.0058844658 29.189463 5 GTCGGGA 65 0.0058844658 29.189463 9 CATTCCG 240 0.0 27.669178 9 CGGATTA 125 7.472074E-6 26.562408 9 ATCAACG 12020 0.0 26.478754 3 TCAACGC 12055 0.0 26.401873 4 GTACATG 6195 0.0 26.34849 1 TATCAAC 12195 0.0 26.181366 2 CAACGCA 12165 0.0 26.124151 5 CATGGGT 860 0.0 25.922619 4 AACGCAG 12280 0.0 25.8795 6 CTGCACG 95 0.0012366673 24.964672 4 ACATGGG 6280 0.0 24.924921 3 TACATGG 6650 0.0 24.255838 2 >>END_MODULE