FastQCFastQC Report
Fri 27 May 2016
SRR939799_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939799_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2167736
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50510.23300807847450059No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40830.18835319430041295No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25080.11569674536013609No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39800.043.631081
GTATCAA65500.036.0008741
ATCAACG78900.030.1887253
TCAACGC79250.029.815914
CAACGCA80100.029.4995145
TATCAAC80750.029.496412
AACGCAG84450.028.092376
ACGCAGA96550.024.4734447
CGCAGAG97900.023.9905688
TACATGG69500.022.3239442
GTACATG70750.022.0631451
GCAGAGT105700.021.4122059
GAGTACT57950.021.3690412-13
ACATGGG71150.021.3399353
GTACTTT62600.019.47990414-15
CAGAGTA101350.019.14685810-11
AGAGTAC90800.019.07238410-11
GTGTACG3301.2289092E-817.2530251
TACCTGG15100.016.9677732
GTATAGG6800.016.7455841
Produced by FastQC (version 0.11.5)