FastQCFastQC Report
Fri 27 May 2016
SRR939799_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939799_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2167736
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55810.2574575501813874No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50250.23180867042850237No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32130.14821915583816478No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41600.043.2842671
GTATCAA63650.033.917751
ATCAACG82400.025.9650043
TCAACGC82800.025.8389764
TATCAAC84450.025.392862
CAACGCA84550.025.3041635
AACGCAG87550.024.476796
GTACATG71850.022.0430931
TACATGG69950.021.7627942
ACGCAGA101550.020.8704517
ACATGGG71050.020.627953
CGCAGAG102500.020.5855968
TGGTATC23450.019.0932832
AGAGTAC98300.019.06947910-11
GTGGTAT25300.019.0037651
TAGCGCG1751.05656116E-418.849934
GAGTACT62350.018.65884412-13
GCAGAGT111850.018.3173479
AGTACTT67350.017.90303412-13
GTACTTT67200.017.31258814-15
Produced by FastQC (version 0.11.5)