##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939799_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2167736 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.3747919488351 31.0 31.0 34.0 30.0 34.0 2 31.58552609727384 31.0 31.0 34.0 30.0 34.0 3 31.64356268475497 31.0 31.0 34.0 30.0 34.0 4 35.46179839242417 37.0 35.0 37.0 33.0 37.0 5 35.184046396793704 37.0 35.0 37.0 32.0 37.0 6 35.205537482424056 37.0 35.0 37.0 32.0 37.0 7 35.12220307269889 37.0 35.0 37.0 32.0 37.0 8 35.0615720733521 37.0 35.0 37.0 32.0 37.0 9 36.66594917462274 39.0 35.0 39.0 32.0 39.0 10-11 36.51918476235114 38.0 35.0 39.0 32.0 39.0 12-13 36.46239279137312 38.0 35.0 39.0 32.0 39.0 14-15 37.50037296977123 39.0 36.0 41.0 32.0 41.0 16-17 37.42048985669842 39.0 36.0 41.0 32.0 41.0 18-19 37.38513246077936 39.0 36.0 41.0 32.0 41.0 20-21 37.30275158045076 39.0 36.0 40.5 31.0 41.0 22-23 37.16375333527699 39.0 36.0 40.0 31.0 41.0 24-25 37.01378281303627 39.0 36.0 40.0 31.0 41.0 26-27 36.71853099270391 39.0 35.5 40.0 30.0 41.0 28-29 36.49079615783472 38.0 35.0 40.0 30.0 41.0 30-31 36.249138502105424 38.0 35.0 40.0 30.0 41.0 32-33 36.228086123033435 38.0 35.0 40.0 30.0 41.0 34-35 36.319111736853564 38.0 35.0 40.0 30.0 41.0 36-37 36.301611220185485 38.0 35.0 40.0 30.0 41.0 38-39 36.180245657220254 38.0 35.0 40.0 30.0 41.0 40-41 36.015421388951424 38.0 35.0 40.0 29.5 41.0 42-43 35.834437865127484 38.0 35.0 40.0 29.0 41.0 44-45 35.60102959954533 38.0 34.0 40.0 27.5 41.0 46-47 35.33122206763185 38.0 34.0 40.0 27.0 41.0 48-49 35.0407849479826 38.0 34.0 40.0 26.0 41.0 50-51 34.792825556248545 38.0 33.0 40.0 26.0 41.0 52-53 34.51821070462455 38.0 33.0 40.0 24.5 41.0 54-55 34.22301654814055 38.0 33.0 40.0 24.0 41.0 56-57 33.016372381138666 36.5 32.0 39.5 18.0 41.0 58-59 29.68972905372241 34.5 24.5 39.0 8.0 40.0 60-61 29.68269129635712 34.0 25.0 38.0 9.5 40.0 62-63 31.485112808939835 35.0 29.5 38.5 13.0 40.0 64-65 31.002677447807297 35.0 29.0 38.0 7.5 40.0 66-67 25.506513016345167 28.5 18.0 36.0 2.0 39.0 68-69 17.67199188462064 13.0 7.0 29.0 2.0 35.5 70-71 16.51581580967424 15.0 7.5 26.0 2.0 33.5 72-73 18.658029852343645 19.0 7.5 30.0 2.0 34.5 74-75 19.414497198920902 19.0 8.5 30.5 2.0 35.0 76-77 20.495208826167023 23.5 9.5 31.5 2.0 34.5 78-79 23.652318363490757 29.0 12.0 34.0 2.0 36.0 80-81 26.347571844541953 31.5 23.0 35.0 2.0 36.0 82-83 27.24363921621452 33.0 26.0 35.0 2.0 36.0 84-85 27.198402849793517 33.0 26.0 35.0 2.0 36.0 86-87 26.89390797587898 33.0 26.0 35.0 2.0 36.0 88-89 26.447848354227638 33.0 25.0 35.0 2.0 35.0 90-91 24.86289405167419 31.5 14.5 34.5 2.0 35.0 92-93 22.804261681311743 31.0 2.0 34.0 2.0 35.0 94-95 18.262693196957564 24.0 2.0 33.0 2.0 35.0 96-97 16.916755084567495 15.0 2.0 32.5 2.0 34.5 98-99 15.786036906708198 4.5 2.0 32.0 2.0 34.0 100-101 15.150150433447616 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5837884553991408 1101 2 0.5753344148818371 1101 3 0.6032461428445579 1101 4 0.45923006374842856 1101 5 0.564487700151524 1101 6 0.6362252749562742 1101 7 0.6429130034094541 1101 8 0.5461532238316309 1101 9 0.5974298821997266 1101 10-11 0.5232798877621363 1101 12-13 0.7129039546652933 1101 14-15 0.8639756243643788 1101 16-17 0.8275895362792696 1101 18-19 0.8000607407537785 1101 20-21 0.8911537761982373 1101 22-23 0.7171563419359828 1101 24-25 0.730782995417421 1101 26-27 0.7890939007551054 1101 28-29 0.831544589120945 1101 30-31 0.8068270100976491 1101 32-33 0.9600985929004793 1101 34-35 1.1486090665030062 1101 36-37 0.9354922839959912 1101 38-39 0.9858252243997541 1101 40-41 0.9250676697689713 1101 42-43 1.0317818548017499 1101 44-45 0.9714192421134129 1101 46-47 0.903460109682996 1101 48-49 1.0192487045833971 1101 50-51 0.8775932943512785 1101 52-53 0.9530240986840539 1101 54-55 0.9532737000782845 1101 56-57 1.6576514983706332 1101 58-59 -7.877851843151795 1101 60-61 -9.197498365798655 1101 62-63 -2.011643803029976 1101 64-65 0.47268245807809706 1101 66-67 -0.9622347667279065 1101 68-69 -3.125728781623053 1101 70-71 -4.039477213587325 1101 72-73 -6.50555686084363 1101 74-75 -7.458355367448856 1101 76-77 -8.390746634344616 1101 78-79 -11.4816707047101 1101 80-81 -7.439288946740479 1101 82-83 -3.3751155610484425 1101 84-85 -2.34181686170443 1101 86-87 -2.0751767442826115 1101 88-89 -2.308116671095984 1101 90-91 -7.963118494660723 1101 92-93 -13.996236898869471 1101 94-95 -12.701261286320388 1101 96-97 -12.362388698044064 1101 98-99 -11.904927173801571 1101 100-101 -11.696487985416734 1102 1 0.4558835852498504 1102 2 0.5019053136247749 1102 3 0.457788567579005 1102 4 0.3340713288421284 1102 5 0.4398733212899728 1102 6 0.5202701736479369 1102 7 0.41549010627309 1102 8 0.49208422648931816 1102 9 0.6272643535248932 1102 10-11 0.6656565530899812 1102 12-13 0.6491057680460273 1102 14-15 0.6810429382193917 1102 16-17 0.7359862496335055 1102 18-19 0.6596865280190869 1102 20-21 0.7768323839456954 1102 22-23 0.9518092615473321 1102 24-25 0.825190954808626 1102 26-27 0.9379299872026081 1102 28-29 0.8099797150406758 1102 30-31 0.942883761565362 1102 32-33 0.7014905890720371 1102 34-35 0.765055113726099 1102 36-37 0.797951371836426 1102 38-39 0.6715617287281148 1102 40-41 0.9126284504968893 1102 42-43 0.9591959926767899 1102 44-45 0.8997701936682887 1102 46-47 0.5617297069278848 1102 48-49 0.6768558025760356 1102 50-51 0.6663342253112106 1102 52-53 0.7484276228980775 1102 54-55 0.7914801024573848 1102 56-57 1.2226394490453103 1102 58-59 -9.029222133446535 1102 60-61 -9.606195244083377 1102 62-63 -2.579908358358601 1102 64-65 0.2905883109737779 1102 66-67 -1.029150182173261 1102 68-69 -3.4885128166942803 1102 70-71 -4.284546390387675 1102 72-73 -6.630556647203111 1102 74-75 -7.586219432258844 1102 76-77 -8.880762358286884 1102 78-79 -12.111992490061196 1102 80-81 -8.018539324884198 1102 82-83 -3.9204684524592395 1102 84-85 -2.9372131221407756 1102 86-87 -2.611192904051535 1102 88-89 -2.884055621181016 1102 90-91 -8.516810630125907 1102 92-93 -14.441068080130634 1102 94-95 -12.483963454344384 1102 96-97 -12.398013895661546 1102 98-99 -11.946307847025578 1102 100-101 -11.586194571526683 1103 1 0.18127449153737274 1103 2 0.15429721729155332 1103 3 0.12398203226614513 1103 4 0.10515115363276095 1103 5 0.20532723808316433 1103 6 0.1570819126421057 1103 7 0.20039648958434242 1103 8 0.29670418466157145 1103 9 0.25870625135191716 1103 10-11 0.288521262417369 1103 12-13 0.27343577202042013 1103 14-15 0.4105510798422003 1103 16-17 0.30371025454393674 1103 18-19 0.36644547397323635 1103 20-21 0.3207333509918442 1103 22-23 0.33133076317867705 1103 24-25 0.3449212296803026 1103 26-27 0.25321085407389887 1103 28-29 0.2936607587010158 1103 30-31 0.36032636604440427 1103 32-33 0.2497431167604489 1103 34-35 0.44167316620657004 1103 36-37 0.09204297107987003 1103 38-39 0.14326539869724542 1103 40-41 0.2536763835187088 1103 42-43 0.24010036022323078 1103 44-45 0.16649856284817588 1103 46-47 0.12479167804170288 1103 48-49 0.308557233273298 1103 50-51 0.4488060831674403 1103 52-53 0.420109509331283 1103 54-55 0.30056333278981384 1103 56-57 0.8966614376027593 1103 58-59 -6.821467278679911 1103 60-61 -8.895149962056959 1103 62-63 -2.9296399818349066 1103 64-65 -0.4701428638079044 1103 66-67 -1.8464383063020229 1103 68-69 -4.2561762814402435 1103 70-71 -4.737531554056183 1103 72-73 -7.617776622684556 1103 74-75 -8.333701721845014 1103 76-77 -9.192625919829887 1103 78-79 -12.25635988105319 1103 80-81 -8.871480921480842 1103 82-83 -5.028451981837392 1103 84-85 -4.118663569533005 1103 86-87 -3.832612043650183 1103 88-89 -3.9319327662396155 1103 90-91 -9.354788176909043 1103 92-93 -15.087011468985025 1103 94-95 -12.923750463038473 1103 96-97 -12.722864078710467 1103 98-99 -12.300956730876688 1103 100-101 -11.98624500765954 1104 1 0.08022951265843759 1104 2 0.06160543028098786 1104 3 0.10636194731025483 1104 4 0.0780049579911335 1104 5 0.0777163554427105 1104 6 0.14827431903579935 1104 7 0.1319468992442836 1104 8 0.15819654880769463 1104 9 0.039563439869503725 1104 10-11 0.21722907629514765 1104 12-13 0.10672012792092289 1104 14-15 0.2014743098930154 1104 16-17 0.24396812616974017 1104 18-19 0.10337634486636205 1104 20-21 0.06361371018871154 1104 22-23 0.13361167850522548 1104 24-25 0.2214641775376549 1104 26-27 0.15598139861044302 1104 28-29 0.13019893942156813 1104 30-31 0.16056592011032222 1104 32-33 0.20741827049063488 1104 34-35 0.15541812039725045 1104 36-37 0.02062335293332751 1104 38-39 0.048597735067723136 1104 40-41 0.16827162222292458 1104 42-43 0.04203915911156031 1104 44-45 -0.0476716787175917 1104 46-47 -0.04383816739096602 1104 48-49 0.0015975219281543218 1104 50-51 -0.13362611075127262 1104 52-53 -0.14637871622176846 1104 54-55 -0.17054968662719716 1104 56-57 0.42535885738089263 1104 58-59 -6.634287956153901 1104 60-61 -8.929025384872787 1104 62-63 -3.028082535750446 1104 64-65 -0.6912347957191116 1104 66-67 -2.249749779374504 1104 68-69 -4.690135111010203 1104 70-71 -5.211811327301552 1104 72-73 -8.263101372994882 1104 74-75 -9.169392683676536 1104 76-77 -9.546108478331163 1104 78-79 -12.567940613185996 1104 80-81 -9.086530217266986 1104 82-83 -5.233702979389793 1104 84-85 -4.300406124917867 1104 86-87 -4.1721743994501885 1104 88-89 -4.200323746918382 1104 90-91 -9.903151849948037 1104 92-93 -15.420601571044925 1104 94-95 -12.81563468474081 1104 96-97 -12.430397878127563 1104 98-99 -11.759055720798765 1104 100-101 -11.32623673228737 1105 1 0.06027869468360336 1105 2 0.12103929801552837 1105 3 0.043222480503860794 1105 4 0.10520009527907348 1105 5 0.05770775034784492 1105 6 -0.04030746464885482 1105 7 0.009886425393609954 1105 8 0.10238363683136953 1105 9 0.014678120446568244 1105 10-11 0.16543273959107552 1105 12-13 0.09359272788014295 1105 14-15 0.11877538467788185 1105 16-17 0.14025080298026182 1105 18-19 0.19925036301781773 1105 20-21 0.29934274564203633 1105 22-23 0.2429467617510923 1105 24-25 0.2468190173108198 1105 26-27 0.31675433467955827 1105 28-29 0.2712199785636429 1105 30-31 0.1989357343413971 1105 32-33 0.2740720091413493 1105 34-35 0.2699243174176189 1105 36-37 0.1536550449087173 1105 38-39 0.2879014224971925 1105 40-41 0.33323921791015465 1105 42-43 0.2020505831806716 1105 44-45 0.19881548485533784 1105 46-47 0.2384196865176449 1105 48-49 0.23607672569571747 1105 50-51 0.18890871756449457 1105 52-53 0.28019080140580854 1105 54-55 0.07366581339715594 1105 56-57 0.716702439408138 1105 58-59 -6.339759877818274 1105 60-61 -8.539571960272848 1105 62-63 -2.423067965802378 1105 64-65 -0.1333263597812291 1105 66-67 -1.7469132096846245 1105 68-69 -4.079664751635439 1105 70-71 -4.53921397961923 1105 72-73 -6.2473341017304715 1105 74-75 -6.605421600944087 1105 76-77 -8.558563362241099 1105 78-79 -11.927873339347094 1105 80-81 -8.21036690419804 1105 82-83 -4.203400673109538 1105 84-85 -3.2656920868891284 1105 86-87 -3.0121072256274566 1105 88-89 -3.186928155371824 1105 90-91 -8.669805977550517 1105 92-93 -11.80412338855357 1105 94-95 -11.898676998803804 1105 96-97 -12.236325855002342 1105 98-99 -11.496619126882749 1105 100-101 -11.055253293668498 1106 1 0.11501850991836093 1106 2 0.12086794589600203 1106 3 0.12350265067580679 1106 4 0.09498818982459056 1106 5 0.005865412454326702 1106 6 -0.03217693896854712 1106 7 -0.010660887650075779 1106 8 0.006064832619514959 1106 9 -0.00547542315687366 1106 10-11 0.0779238173656509 1106 12-13 0.06115289687191705 1106 14-15 0.06738128577253377 1106 16-17 -0.026741294017888606 1106 18-19 0.052915558376255944 1106 20-21 0.1629535508873019 1106 22-23 0.02910234266692413 1106 24-25 0.07546765430495128 1106 26-27 0.06482889289936367 1106 28-29 0.11580842897686949 1106 30-31 0.18417817489488897 1106 32-33 0.2587346705310267 1106 34-35 0.19684689387262466 1106 36-37 0.07562174358884732 1106 38-39 0.14206611117536028 1106 40-41 0.22613657799055886 1106 42-43 0.23946936586948908 1106 44-45 0.13008843819044102 1106 46-47 0.041979990170240455 1106 48-49 0.06826739634824008 1106 50-51 0.10124905843039045 1106 52-53 -0.03331388278618874 1106 54-55 -0.06552619894624456 1106 56-57 0.7942172398056186 1106 58-59 -6.725151126150617 1106 60-61 -9.210581659256729 1106 62-63 -2.6452676920172493 1106 64-65 -0.18899565624271375 1106 66-67 -1.4533984713219148 1106 68-69 -3.9369413914998095 1106 70-71 -4.4807729775157235 1106 72-73 -6.193921887161304 1106 74-75 -6.56532851475624 1106 76-77 -8.305562146889311 1106 78-79 -11.990918625858809 1106 80-81 -7.970627888984769 1106 82-83 -4.080037738350814 1106 84-85 -3.0426335196425 1106 86-87 -2.806248742052105 1106 88-89 -3.0674670748094712 1106 90-91 -8.561900195494527 1106 92-93 -11.926775949624714 1106 94-95 -11.846523163183065 1106 96-97 -12.40974185974422 1106 98-99 -11.896745711842572 1106 100-101 -11.505824448487218 1107 1 0.06743296509770147 1107 2 0.042619650212905924 1107 3 0.014350106275344388 1107 4 0.006927081147566128 1107 5 0.023729524676809888 1107 6 0.006824216931406113 1107 7 0.05186761732119294 1107 8 -0.012998918328158027 1107 9 -0.05832681748922397 1107 10-11 0.09195349692865307 1107 12-13 0.11402952511325282 1107 14-15 0.13785062152632577 1107 16-17 0.08826514343266467 1107 18-19 0.013404303679010354 1107 20-21 0.09840764162170501 1107 22-23 0.09642155198949354 1107 24-25 0.07170312891926045 1107 26-27 0.23333701186285793 1107 28-29 0.2423768566090061 1107 30-31 0.11020655949106128 1107 32-33 0.053319249360257004 1107 34-35 0.044865489955128623 1107 36-37 -0.03125750598834287 1107 38-39 0.12509228864562516 1107 40-41 -0.0028260055430209263 1107 42-43 0.13781914298865416 1107 44-45 0.11336826317553061 1107 46-47 0.09298108476114209 1107 48-49 0.10994652420453832 1107 50-51 0.12901402195149814 1107 52-53 0.11462093671846674 1107 54-55 0.044595701395138576 1107 56-57 0.7005932691621837 1107 58-59 -6.9995050259297535 1107 60-61 -9.08894561970192 1107 62-63 -2.844218746458189 1107 64-65 -0.46271085774762355 1107 66-67 -1.834113858249328 1107 68-69 -4.13640647001278 1107 70-71 -4.912181231508663 1107 72-73 -6.250146213995379 1107 74-75 -6.851589268139826 1107 76-77 -9.26462882123207 1107 78-79 -12.649748512676315 1107 80-81 -8.714567264798799 1107 82-83 -4.684848368144319 1107 84-85 -3.7011241505724684 1107 86-87 -3.4078124219709984 1107 88-89 -3.4930655483635853 1107 90-91 -8.733088903264179 1107 92-93 -11.994686567006877 1107 94-95 -12.221360731576304 1107 96-97 -12.60549904897835 1107 98-99 -11.864286493577858 1107 100-101 -11.390297631757921 1108 1 0.0055740982889034285 1108 2 -0.026397271008459455 1108 3 -0.01924339863098723 1108 4 -0.05043652894592299 1108 5 -0.17559252007813342 1108 6 -0.08328672781677682 1108 7 -7.026657618780519E-4 1108 8 -0.06336206184519 1108 9 0.013331974638226995 1108 10-11 0.017152228655923807 1108 12-13 -0.0837010826662663 1108 14-15 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8.813744308775519 2316 96-97 9.67955926191238 2316 98-99 10.0607607592532 2316 100-101 8.846418864713817 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 12.0 4 38.0 5 93.0 6 182.0 7 511.0 8 1181.0 9 1980.0 10 3428.0 11 5222.0 12 7261.0 13 9786.0 14 13092.0 15 17910.0 16 24423.0 17 30927.0 18 34848.0 19 36265.0 20 38038.0 21 37768.0 22 36210.0 23 34840.0 24 36591.0 25 40190.0 26 53662.0 27 76696.0 28 106441.0 29 138083.0 30 164804.0 31 178915.0 32 185859.0 33 192096.0 34 193641.0 35 185901.0 36 154516.0 37 95348.0 38 29746.0 39 1226.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.63887484173886 19.939611609631136 14.714825423659994 23.70668812497001 2 15.75147695325511 20.968676504102625 39.532615685829455 23.747230856812806 3 19.324681291975025 24.52125750464326 30.203703720789903 25.950357482591812 4 13.311307280960413 17.37107286127093 36.931757372669 32.38586248509966 5 13.42087842996777 37.59732148380122 34.86248738617553 14.119312700055486 6 31.96165582970243 36.74784971410251 17.337174516257754 13.953319939937309 7 28.072099185509675 32.49284045658696 21.135415013636347 18.299645344267017 8 25.749493018509668 36.240734555747665 19.63992781395486 18.3698446117878 9 25.698193876007043 16.781932855292343 20.179717456369225 37.34015581233139 10-11 24.63752504917573 26.911879490860514 27.755386264748104 20.695209195215654 12-13 25.450446917890368 24.00834326689228 28.148699841678138 22.392509973539212 14-15 22.23585398741818 25.7058417303623 26.132601323688228 25.925702958531293 16-17 21.937726734251772 28.86400834788 26.850732746053946 22.34753217181428 18-19 21.89406681533381 27.620466059511763 28.84284123400498 21.642625891149443 20-21 22.564140651813688 26.74841401351456 28.99769621393011 21.689749120741638 22-23 22.239608513213785 26.74297054622888 28.86428513435215 22.153135806205185 24-25 22.129885742544296 26.845750589555184 28.976683507585793 22.047680160314727 26-27 21.977645325107847 27.106825120880806 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22.354753212428154 52-53 22.218033655619692 27.297429422332325 28.047380224618422 22.43715669742956 54-55 22.025352139969208 27.292837332996218 28.143191514590583 22.538619012443995 56-57 22.066350308023488 27.240969329781827 28.22608085417296 22.46659950802173 58-59 20.67496073079993 27.016489112704345 29.7828119211018 22.525738235393927 60-61 22.106305302030673 27.04725824091793 28.3943933230003 22.45204313405109 62-63 21.84840029551671 27.206059245888035 28.27277493468393 22.67276552391132 64-65 21.365633118806837 27.091136685509053 28.660619639003215 22.882610556680895 66-67 21.733218977324984 26.65425599754751 29.07564696755714 22.536878057570373 68-69 22.46223847667772 25.84487679841204 29.618360555566493 22.074524169343743 70-71 21.522580535580452 26.198630015803154 29.750152570389382 22.528636878227008 72-73 21.2607962923952 26.327553680520026 29.72083872520991 22.69081130187487 74-75 21.737570376974265 26.42963427527097 29.057361613727057 22.77543373402771 76-77 20.01518286510556 26.933604826975294 29.65648884346368 23.39472346445546 78-79 20.91856413573239 27.06229372545468 28.48720533159252 23.531936807220415 80-81 21.331341695842728 27.120414645639762 27.83989983994782 23.708343818569695 82-83 21.43018601930497 27.062916449081342 27.726882289877164 23.780015241736525 84-85 21.38347168819067 26.961759624677374 27.730419077977704 23.924349609154255 86-87 21.243633149464888 27.008178816638857 27.794367508383417 23.953820525512842 88-89 21.342756069640416 26.891818674372402 27.63058500448971 24.134840251497472 90-91 21.0856724071364 26.873397558161848 27.877159033899517 24.16377100080223 92-93 20.80786230022145 26.80951395076288 27.99136372542858 24.391260023587087 94-95 19.878959106277495 26.55011482851449 29.112218754280068 24.458707310927945 96-97 19.585503827204228 26.409648723501906 29.405654672049398 24.59919277724447 98-99 19.82355807536289 26.51112508817552 28.76965349904466 24.895663337416927 100-101 19.614948843276252 26.615716842624053 28.756457123587676 25.01287719051201 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 218.0 1 161.0 2 117.5 3 134.0 4 157.5 5 198.5 6 251.0 7 379.5 8 552.0 9 722.0 10 880.5 11 1049.5 12 1190.5 13 1333.5 14 1571.0 15 1793.5 16 2027.0 17 2211.5 18 2413.5 19 2802.5 20 3283.5 21 3960.0 22 4852.0 23 5850.0 24 7170.0 25 9075.5 26 11584.5 27 14549.5 28 17695.5 29 21224.0 30 25248.0 31 29645.5 32 34358.5 33 39154.0 34 43719.5 35 48611.0 36 53860.0 37 58842.5 38 63411.0 39 67450.5 40 71081.5 41 74470.0 42 78101.0 43 81347.5 44 84245.0 45 86740.0 46 88231.0 47 89266.5 48 89711.5 49 88591.0 50 85922.5 51 82919.5 52 79513.0 53 75337.0 54 70240.0 55 63823.5 56 56814.0 57 49525.5 58 42363.5 59 35373.0 60 28464.0 61 22414.0 62 17183.0 63 12567.0 64 8947.5 65 6437.0 66 4593.0 67 3243.5 68 2241.0 69 1489.5 70 999.0 71 676.5 72 462.0 73 318.5 74 193.0 75 119.0 76 77.0 77 41.5 78 23.0 79 13.0 80 7.5 81 5.5 82 1.5 83 0.5 84 1.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01992862599504737 2 0.004520845711839449 3 0.003598224137994664 4 0.0 5 0.06744363704805383 6 4.613107869223928E-5 7 0.0 8 1.845243147689571E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.306553934611964E-4 16-17 0.0 18-19 0.0013839323607671783 20-21 0.0 22-23 0.0 24-25 0.0 26-27 6.919661803835891E-5 28-29 0.0024449471706886818 30-31 0.008741839412179342 32-33 2.7678647215343565E-4 34-35 0.0010610148099215034 36-37 0.025787272988961756 38-39 0.024103488616695024 40-41 0.026363911472614746 42-43 0.027286533046459532 44-45 0.0280707613842276 46-47 0.029224038351533584 48-49 0.029154841733495224 50-51 0.024357209549502337 52-53 0.021243361737776186 54-55 0.01591522214882255 56-57 0.14805769706274197 58-59 0.7638845320647901 60-61 0.30933194817081044 62-63 3.459830901917946E-4 64-65 0.14768864843320403 66-67 1.4362219384648316 68-69 2.7618446157650194 70-71 2.2245559422365084 72-73 1.8950647126771896 74-75 1.582964899784845 76-77 1.1316184258599755 78-79 0.43162543778393675 80-81 2.53720932807316E-4 82-83 1.845243147689571E-4 84-85 4.613107869223928E-5 86-87 0.0029062579576110744 88-89 2.306553934611964E-5 90-91 0.23162414611373341 92-93 0.17714334217819883 94-95 1.3128443685024376 96-97 1.2941612816320807 98-99 1.0221493761232916 100-101 0.4096439787870848 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2167736.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.7630070596096 #Duplication Level Percentage of deduplicated Percentage of total 1 79.0181915799215 51.964738907079635 2 12.968737235005525 17.057263166797814 3 3.89740770576296 7.689157514047993 4 1.5847041829055049 4.168596494712305 5 0.7679654827607912 2.525185973216721 6 0.4332198972945699 1.709390589848769 7 0.2786515683584705 1.2827475539990572 8 0.19479966974115612 1.0248489645117818 9 0.13239519657087898 0.7836035622074204 >10 0.6751704726492613 7.784153239808678 >50 0.03422144453239894 1.5253997447389407 >100 0.013904326370205066 1.6462311440715292 >500 3.5068784821232684E-4 0.14035708134587557 >1k 1.402751392848701E-4 0.20912882248503264 >5k 1.402751392848701E-4 0.48919724112842 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5581 0.2574575501813874 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5025 0.23180867042850237 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3213 0.14821915583816478 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.613107869223928E-5 0.0 0.0 0.0 0.0 7 4.613107869223928E-5 0.0 0.0 0.0 0.0 8 4.613107869223928E-5 0.0 0.0 0.0 0.0 9 4.613107869223928E-5 0.0 0.0 9.226215738447856E-5 0.0 10-11 4.613107869223928E-5 0.0 0.0 1.1532769673059818E-4 0.0 12-13 4.613107869223928E-5 0.0 0.0 1.845243147689571E-4 2.306553934611964E-5 14-15 4.613107869223928E-5 0.0 0.0 2.3065539346119637E-4 4.613107869223928E-5 16-17 4.613107869223928E-5 0.0 0.0 2.3065539346119637E-4 4.613107869223928E-5 18-19 4.613107869223928E-5 0.0 0.0 2.53720932807316E-4 9.226215738447856E-5 20-21 4.613107869223928E-5 0.0 0.0 3.459830901917946E-4 9.226215738447856E-5 22-23 4.613107869223928E-5 0.0 0.0 5.305074049607517E-4 9.226215738447856E-5 24-25 4.613107869223928E-5 0.0 0.0 8.995560344986659E-4 9.226215738447856E-5 26-27 4.613107869223928E-5 0.0 0.0 0.002098964080496887 9.226215738447856E-5 28-29 6.919661803835891E-5 0.0 0.0 0.0074963002874888825 9.226215738447856E-5 30-31 9.226215738447856E-5 0.0 0.0 0.024057357538002783 9.226215738447856E-5 32-33 9.226215738447856E-5 0.0 0.0 0.0641221993822126 9.226215738447856E-5 34-35 9.226215738447856E-5 0.0 0.0 0.1232161111869711 9.226215738447856E-5 36-37 1.3839323607671783E-4 0.0 0.0 0.22532725387224276 9.226215738447856E-5 38-39 2.3065539346119637E-4 0.0 0.0 0.38284182206689377 9.226215738447856E-5 40-41 2.3065539346119637E-4 0.0 0.0 0.54252455096008 9.226215738447856E-5 42-43 2.3065539346119637E-4 0.0 0.0 0.7059900283060299 9.226215738447856E-5 44-45 2.7678647215343565E-4 0.0 0.0 0.9014012776463554 1.1532769673059818E-4 46-47 2.7678647215343565E-4 0.0 0.0 1.12861990574498 1.3839323607671783E-4 48-49 2.7678647215343565E-4 0.0 0.0 1.3486882166463077 1.3839323607671783E-4 50-51 2.7678647215343565E-4 0.0 0.0 1.5691025106378267 1.3839323607671783E-4 52-53 2.7678647215343565E-4 0.0 0.0 1.7766462336742113 1.845243147689571E-4 54-55 2.7678647215343565E-4 0.0 0.0 2.00693257850587 1.845243147689571E-4 56-57 2.7678647215343565E-4 0.0 0.0 2.211523912505951 1.845243147689571E-4 58-59 2.7678647215343565E-4 0.0 0.0 2.352638882225511 1.845243147689571E-4 60-61 2.7678647215343565E-4 0.0 0.0 2.456825923451933 1.845243147689571E-4 62-63 4.3824524757627315E-4 0.0 0.0 2.546504740429646 2.0758985411507673E-4 64-65 4.6131078692239274E-4 0.0 0.0 2.644118102942425 2.3065539346119637E-4 66-67 4.6131078692239274E-4 0.0 0.0 2.723232902899615 2.3065539346119637E-4 68-69 4.6131078692239274E-4 0.0 0.0 2.8187473013318964 2.3065539346119637E-4 70-71 4.843763262685124E-4 0.0 0.0 2.9836197765779593 2.7678647215343565E-4 72-73 5.07441865614632E-4 0.0 0.0 3.1909789752995756 2.998520114995553E-4 74-75 5.07441865614632E-4 0.0 0.0 3.425786165843073 3.459830901917946E-4 76-77 5.07441865614632E-4 0.0 0.0 3.774306465362941 3.690486295379142E-4 78-79 5.07441865614632E-4 0.0 0.0 4.266732664863249 3.690486295379142E-4 80-81 5.07441865614632E-4 0.0 0.0 4.809095757047906 3.690486295379142E-4 82-83 5.07441865614632E-4 0.0 0.0 5.324103119568066 4.151797082301535E-4 84-85 5.535729443068713E-4 0.0 0.0 5.737875829898106 4.151797082301535E-4 86-87 6.458351016913499E-4 0.0 0.0 6.1075241634590185 4.151797082301535E-4 88-89 6.919661803835892E-4 0.0 0.0 6.494656175844291 4.3824524757627315E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4160 0.0 43.284267 1 GTATCAA 6365 0.0 33.91775 1 ATCAACG 8240 0.0 25.965004 3 TCAACGC 8280 0.0 25.838976 4 TATCAAC 8445 0.0 25.39286 2 CAACGCA 8455 0.0 25.304163 5 AACGCAG 8755 0.0 24.47679 6 GTACATG 7185 0.0 22.043093 1 TACATGG 6995 0.0 21.762794 2 ACGCAGA 10155 0.0 20.870451 7 ACATGGG 7105 0.0 20.62795 3 CGCAGAG 10250 0.0 20.585596 8 TGGTATC 2345 0.0 19.093283 2 AGAGTAC 9830 0.0 19.069479 10-11 GTGGTAT 2530 0.0 19.003765 1 TAGCGCG 175 1.05656116E-4 18.84993 4 GAGTACT 6235 0.0 18.658844 12-13 GCAGAGT 11185 0.0 18.317347 9 AGTACTT 6735 0.0 17.903034 12-13 GTACTTT 6720 0.0 17.312588 14-15 >>END_MODULE