FastQCFastQC Report
Fri 27 May 2016
SRR939798_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939798_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2315197
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53400.23064991877581043No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42910.18534059952565593No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28170.1216743110845427No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42250.042.191541
GTATCAA67800.036.2120971
ATCAACG82600.029.3276753
TATCAAC83100.029.2065542
TCAACGC84400.028.7585564
CAACGCA86000.028.2247375
AACGCAG89600.027.1968846
TACATGG73750.025.0646822
GTACATG76100.024.907681
ACGCAGA100550.023.8140437
ACATGGG77250.023.5647563
CGCAGAG101150.023.3477298
GCAGAGT107550.020.5157819
GAGTACT57550.019.53664612-13
CATGGGA42000.019.0396754
AGAGTAC91650.018.863510-11
CAGAGTA103050.018.21471210-11
GTACTTT62100.018.11089714-15
AGTACTT63650.017.47954612-13
GAGTACA56550.017.1335561
Produced by FastQC (version 0.11.5)