##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939798_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2315197 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.86331271161806 31.0 30.0 33.0 26.0 34.0 2 29.951571291773444 31.0 30.0 33.0 25.0 34.0 3 29.89015664757686 31.0 30.0 34.0 25.0 34.0 4 33.51445341368359 35.0 33.0 37.0 28.0 37.0 5 33.37071229791677 35.0 33.0 37.0 27.0 37.0 6 33.338379412205526 35.0 33.0 37.0 26.0 37.0 7 33.325526078342364 35.0 33.0 37.0 26.0 37.0 8 33.160903370209965 35.0 33.0 37.0 26.0 37.0 9 34.439464114716806 37.0 33.0 39.0 27.0 39.0 10-11 34.235891373390686 37.0 33.0 39.0 26.5 39.0 12-13 34.131384067964845 37.0 33.0 39.0 26.0 39.0 14-15 34.72527262258892 38.0 33.0 40.0 25.0 41.0 16-17 34.49932878282064 38.0 33.0 40.0 25.0 41.0 18-19 34.51137808143324 38.0 33.0 40.0 25.0 41.0 20-21 34.61345384431649 38.0 33.0 40.0 25.0 41.0 22-23 34.52009181076168 38.0 33.0 40.0 24.5 41.0 24-25 34.35998232547813 38.0 33.0 40.0 24.0 41.0 26-27 34.251014924431914 38.0 33.0 40.0 23.5 41.0 28-29 34.127717641306546 38.0 32.5 40.0 22.5 41.0 30-31 33.94188485904223 38.0 32.5 40.0 21.5 41.0 32-33 33.66834701323472 38.0 32.0 40.0 19.0 41.0 34-35 33.39394509408919 37.0 31.0 40.0 18.0 41.0 36-37 33.17631501768532 37.0 31.0 40.0 18.0 41.0 38-39 32.90520547495527 37.0 31.0 40.0 16.0 41.0 40-41 32.62059081797359 37.0 30.5 40.0 15.5 41.0 42-43 32.31829321651678 37.0 30.0 40.0 14.0 41.0 44-45 31.926174532879923 36.5 30.0 39.0 9.0 41.0 46-47 31.561672073693945 36.0 30.0 39.0 9.0 40.0 48-49 31.230691815858435 36.0 29.5 39.0 8.0 40.0 50-51 30.51363080549949 35.5 28.0 38.5 7.0 40.0 52-53 30.655414420457525 35.5 28.0 38.5 4.0 40.0 54-55 30.73586675345554 36.0 28.0 39.0 2.0 40.0 56-57 30.503919968797472 35.0 28.0 39.0 2.0 40.0 58-59 29.964966912102945 35.0 27.5 38.5 2.0 40.0 60-61 29.46491313698143 34.0 26.0 38.0 2.0 40.0 62-63 29.01217477389613 34.0 26.0 38.0 2.0 40.0 64-65 28.472307756100236 34.0 26.0 38.0 2.0 40.0 66-67 27.92674273506747 33.5 24.5 37.0 2.0 39.0 68-69 27.367813408535 33.0 23.0 36.5 2.0 39.0 70-71 26.803286934114027 32.0 22.0 36.0 2.0 39.0 72-73 26.31199807187034 32.0 21.0 36.0 2.0 38.5 74-75 25.699840229578733 31.0 19.5 35.0 2.0 38.0 76-77 25.02839995905316 31.0 17.5 35.0 2.0 37.0 78-79 24.357225972563025 30.5 11.5 34.0 2.0 36.5 80-81 23.659479301329434 30.0 7.0 34.0 2.0 36.0 82-83 22.89227914514402 29.5 2.0 34.0 2.0 35.5 84-85 22.126389244630154 29.0 2.0 34.0 2.0 35.0 86-87 21.312760641966968 28.5 2.0 33.5 2.0 35.0 88-89 20.448313469652906 27.0 2.0 33.0 2.0 35.0 90-91 19.484127052687093 26.0 2.0 32.5 2.0 34.0 92-93 18.4904329955507 25.0 2.0 32.0 2.0 34.0 94-95 17.10306336782572 20.5 2.0 31.5 2.0 34.0 96-97 14.574728630004271 2.0 2.0 30.5 2.0 34.0 98-99 13.190872310218094 2.0 2.0 29.5 2.0 34.0 100-101 11.98279757618898 2.0 2.0 28.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8404056692879109 1101 2 0.7820584762095528 1101 3 0.8404666427613527 1101 4 0.9532520423665645 1101 5 0.8690529807171714 1101 6 0.9896442936973813 1101 7 0.8034665949310664 1101 8 0.7826406027991482 1101 9 0.9307854773406063 1101 10-11 1.0512086995096652 1101 12-13 0.882574586349115 1101 14-15 1.0615508600368173 1101 16-17 0.7998448138139977 1101 18-19 0.7325661768885894 1101 20-21 1.1159411418372898 1101 22-23 1.0225691658564102 1101 24-25 0.9387653934996294 1101 26-27 0.879023887172167 1101 28-29 1.1309588191175948 1101 30-31 1.194833497210233 1101 32-33 1.0539503919690887 1101 34-35 0.8543219019201089 1101 36-37 0.8244209205643287 1101 38-39 0.6567396633199536 1101 40-41 0.9348536762310431 1101 42-43 1.0258544333889361 1101 44-45 0.9266838008365959 1101 46-47 0.7630965695325642 1101 48-49 0.6776101335925198 1101 50-51 0.6051546679851505 1101 52-53 0.7842513772416027 1101 54-55 0.5994181043886755 1101 56-57 0.5355201401345546 1101 58-59 0.17440428934613195 1101 60-61 0.2111223433924998 1101 62-63 -5.696022815477875E-4 1101 64-65 -0.016360060500204554 1101 66-67 -0.28144708495104354 1101 68-69 -0.06963361101682253 1101 70-71 -0.06262418521816926 1101 72-73 -0.1898577716522034 1101 74-75 -0.11757713367920886 1101 76-77 -0.7246839104080642 1101 78-79 -0.5361216433661617 1101 80-81 -0.8758432207257698 1101 82-83 -1.076545721630982 1101 84-85 -0.8618108371341258 1101 86-87 -0.9955505672573466 1101 88-89 -0.8295385553013581 1101 90-91 -1.2277225049669553 1101 92-93 -1.0524771582855124 1101 94-95 -0.838359439113745 1101 96-97 0.5966200624015059 1101 98-99 0.6494239439107563 1101 100-101 -0.17768559219763702 1102 1 0.8878114114857105 1102 2 0.8501322449951978 1102 3 0.9131003105242854 1102 4 0.9105976982510029 1102 5 0.9935523770786858 1102 6 0.9336127657288031 1102 7 0.9057659718651365 1102 8 1.0014907755646476 1102 9 1.1165285077445617 1102 10-11 0.945791217584123 1102 12-13 0.9459011200765488 1102 14-15 0.9420970487883267 1102 16-17 0.949551320620543 1102 18-19 1.0089090990539162 1102 20-21 0.8562211745864161 1102 22-23 0.75791185211704 1102 24-25 0.949772429010217 1102 26-27 1.0126430814882497 1102 28-29 1.0994863575214922 1102 30-31 1.0220360838358218 1102 32-33 1.2068471628501314 1102 34-35 1.0703134648580885 1102 36-37 0.7748726086705702 1102 38-39 0.8082029662632095 1102 40-41 0.9527213753276627 1102 42-43 0.8125674483897001 1102 44-45 0.8470618034176702 1102 46-47 0.8561623591888434 1102 48-49 0.7024536732730766 1102 50-51 0.9569246470312613 1102 52-53 1.0039757850514377 1102 54-55 0.9126024014620704 1102 56-57 0.6463162490947703 1102 58-59 0.4668525637726759 1102 60-61 0.7752745435506938 1102 62-63 0.5643277791763417 1102 64-65 0.36848653206462956 1102 66-67 0.15863867334378057 1102 68-69 0.16828459943791785 1102 70-71 0.22921073700266348 1102 72-73 0.3273133147582996 1102 74-75 0.15419895113547 1102 76-77 -0.011398313083500256 1102 78-79 -0.23038638394038102 1102 80-81 -0.4364818286105354 1102 82-83 -0.4723327413220879 1102 84-85 -0.6716216970761195 1102 86-87 -0.7434586476869711 1102 88-89 -0.6621836019966096 1102 90-91 -0.6541729719194826 1102 92-93 -0.7825092135704708 1102 94-95 -0.430652293976852 1102 96-97 0.7284394426659961 1102 98-99 0.7428838331743162 1102 100-101 0.33098876218672046 1103 1 0.7397907279708846 1103 2 0.5751763119120277 1103 3 0.7540149558802263 1103 4 0.7274844291526321 1103 5 0.8410249726831722 1103 6 0.8439438894081448 1103 7 0.6947216670410299 1103 8 0.7535511968047217 1103 9 0.8824331501807308 1103 10-11 0.8960569708310189 1103 12-13 0.6710414313025197 1103 14-15 0.8647827696654105 1103 16-17 0.8101489184779354 1103 18-19 0.8257576206043495 1103 20-21 0.700045047500673 1103 22-23 0.8331425500865421 1103 24-25 0.8161650517217183 1103 26-27 1.0779066358951184 1103 28-29 1.0115390647982139 1103 30-31 0.9299515474486242 1103 32-33 0.867132237051969 1103 34-35 1.103651754593109 1103 36-37 0.9720520556442764 1103 38-39 0.9848944509848749 1103 40-41 1.158118986882748 1103 42-43 0.9956673802617004 1103 44-45 1.0880370971070015 1103 46-47 1.1021625430262816 1103 48-49 0.9314683586406964 1103 50-51 1.1931393619570585 1103 52-53 1.1620138970769247 1103 54-55 1.1345305138119812 1103 56-57 0.9493070319899601 1103 58-59 0.876046598788303 1103 60-61 1.0430039528557522 1103 62-63 0.8819950081693086 1103 64-65 0.8314125573188491 1103 66-67 0.8411266247818574 1103 68-69 0.4959481697052759 1103 70-71 0.8546299584691113 1103 72-73 0.6237366851835411 1103 74-75 0.6686919202543855 1103 76-77 0.5512041943150585 1103 78-79 0.39923063940697645 1103 80-81 0.3174643682973901 1103 82-83 0.33161228407271537 1103 84-85 0.06334211217991381 1103 86-87 -0.19234057358245593 1103 88-89 -0.09120468562822737 1103 90-91 -0.30541946188592206 1103 92-93 -0.3532641906989298 1103 94-95 -0.215310698772484 1103 96-97 1.0311419432157063 1103 98-99 1.3432905284619512 1103 100-101 0.9119197726798731 1104 1 0.48567541793278224 1104 2 0.5154923625846131 1104 3 0.5935028775719395 1104 4 0.7689597462131346 1104 5 0.7911523963556419 1104 6 0.8918990445538668 1104 7 0.7669306746921549 1104 8 0.6145653693430049 1104 9 0.8573070289344855 1104 10-11 0.9080975444409347 1104 12-13 0.8199380086691406 1104 14-15 0.8890669526445194 1104 16-17 1.1129101155967547 1104 18-19 1.0788472253875696 1104 20-21 0.9027436924778343 1104 22-23 0.8734413123891116 1104 24-25 1.0413253750374238 1104 26-27 1.1991762552512455 1104 28-29 1.121599968389404 1104 30-31 1.0538443959511241 1104 32-33 1.2558842522443214 1104 34-35 1.173331646773633 1104 36-37 1.0183844412395189 1104 38-39 0.938443954956746 1104 40-41 1.0330082806537249 1104 42-43 0.7846996668725481 1104 44-45 0.9431975774275223 1104 46-47 0.8621877458657963 1104 48-49 0.6065757285176119 1104 50-51 0.9607181908582199 1104 52-53 1.0019190411966328 1104 54-55 0.6749828910431432 1104 56-57 0.6220669124093874 1104 58-59 0.6028204140978417 1104 60-61 0.9867044383574282 1104 62-63 0.6938678364181854 1104 64-65 0.6632276720287038 1104 66-67 0.7501051408152613 1104 68-69 0.42318536328720313 1104 70-71 0.49453417483314865 1104 72-73 0.4840863527258641 1104 74-75 0.5755904794498328 1104 76-77 0.33151727260122144 1104 78-79 0.38334416173127295 1104 80-81 0.28110276910353704 1104 82-83 0.22849429353439277 1104 84-85 0.1708724729840938 1104 86-87 0.4822329799572529 1104 88-89 0.31151620764347143 1104 90-91 -5.712605543806148E-4 1104 92-93 0.35672179662221737 1104 94-95 0.6194843849788718 1104 96-97 1.9669893338315294 1104 98-99 2.075332836261305 1104 100-101 1.9563200178928337 1105 1 0.8571607151217577 1105 2 0.9155891211719407 1105 3 0.8991997376764687 1105 4 0.8190061427488544 1105 5 0.9408744136928817 1105 6 1.1064445567795573 1105 7 0.9717569718784915 1105 8 1.0755497161300127 1105 9 1.0947843263561055 1105 10-11 1.2779855318358813 1105 12-13 0.8511074921739166 1105 14-15 1.0003472590995912 1105 16-17 1.1397140948597482 1105 18-19 1.203816228271826 1105 20-21 1.0866074796512422 1105 22-23 1.0527989516910807 1105 24-25 1.1657600907495933 1105 26-27 1.3991537839946204 1105 28-29 1.213274764363618 1105 30-31 1.3748515841095568 1105 32-33 1.2971821920266464 1105 34-35 1.389957204259069 1105 36-37 1.197364563430277 1105 38-39 1.2403011382243747 1105 40-41 1.2118422074926016 1105 42-43 1.3253570436573625 1105 44-45 1.4406189346385467 1105 46-47 1.1661384571471558 1105 48-49 1.2472159214003007 1105 50-51 1.4037406013102576 1105 52-53 1.4283505263352012 1105 54-55 1.6129709469093285 1105 56-57 1.3422862847475372 1105 58-59 1.2353365867934123 1105 60-61 1.4365385476100379 1105 62-63 1.5216526199406708 1105 64-65 1.2175898716739226 1105 66-67 1.0784493264436996 1105 68-69 1.1201760654743182 1105 70-71 1.012703639832182 1105 72-73 1.113771940190606 1105 74-75 1.0795084091333074 1105 76-77 1.0279078600527427 1105 78-79 0.8525617125757954 1105 80-81 0.7777392185392422 1105 82-83 0.5577865018244381 1105 84-85 0.5203036858239045 1105 86-87 0.6890467936996174 1105 88-89 0.8144578273187761 1105 90-91 0.762136499431449 1105 92-93 0.7640476906477716 1105 94-95 1.042238250695931 1105 96-97 2.3598913247848667 1105 98-99 2.1752717673168487 1105 100-101 1.5402985406588598 1106 1 1.1168077316795113 1106 2 1.1003747253411653 1106 3 1.1145123613138992 1106 4 1.0753452469658313 1106 5 1.1591611570318037 1106 6 1.1275362497085197 1106 7 1.2025204656960469 1106 8 1.117448423251986 1106 9 1.5123780179480732 1106 10-11 1.4905609648795348 1106 12-13 1.3795112448399394 1106 14-15 1.6844493614475056 1106 16-17 1.801222892160304 1106 18-19 1.6569542883636004 1106 20-21 1.5757646759749733 1106 22-23 1.1829980903787387 1106 24-25 1.3495334028002404 1106 26-27 1.616527372538357 1106 28-29 1.6260334650280157 1106 30-31 1.653080936942935 1106 32-33 1.652147925983904 1106 34-35 1.7569726705910327 1106 36-37 1.7305173576930457 1106 38-39 1.6467032264747914 1106 40-41 1.7729649149420013 1106 42-43 1.5862455505492292 1106 44-45 1.842337466496641 1106 46-47 1.645610664589725 1106 48-49 1.4480372150059502 1106 50-51 1.5590533158993658 1106 52-53 1.7031560034449456 1106 54-55 1.6118945338614026 1106 56-57 1.5409738521086211 1106 58-59 1.635591251477333 1106 60-61 1.853355057157689 1106 62-63 1.6514429308589662 1106 64-65 1.8712258921744294 1106 66-67 1.7813802441006068 1106 68-69 1.7586236113708082 1106 70-71 1.8229702646610804 1106 72-73 1.6753944270821997 1106 74-75 1.567963913301167 1106 76-77 1.692256623337368 1106 78-79 1.345368344771778 1106 80-81 1.483100819652492 1106 82-83 1.584186134379678 1106 84-85 1.75170293183454 1106 86-87 1.65710138289365 1106 88-89 1.6671802382657184 1106 90-91 1.7017213959966604 1106 92-93 1.6992418952071802 1106 94-95 2.0508054435180263 1106 96-97 3.4005198917957866 1106 98-99 3.473942963376823 1106 100-101 2.871911523100639 1107 1 0.5590035115713334 1107 2 0.5975695774051921 1107 3 0.6318142929325354 1107 4 0.3731061196907106 1107 5 0.694613411379116 1107 6 0.9168922823717125 1107 7 0.8181271690919019 1107 8 0.539787591388091 1107 9 0.6590839550404368 1107 10-11 0.850919773713187 1107 12-13 0.9135582285768962 1107 14-15 0.8821543816814668 1107 16-17 0.9171968873867868 1107 18-19 0.8963562553585191 1107 20-21 0.7702046564860225 1107 22-23 0.6714741949280167 1107 24-25 0.6297043059377572 1107 26-27 0.8778756205892293 1107 28-29 0.9087283340032002 1107 30-31 1.0021789329263058 1107 32-33 0.8783049912325893 1107 34-35 0.9473931913995202 1107 36-37 0.7093378371834831 1107 38-39 0.7302984083518922 1107 40-41 0.8553770478215696 1107 42-43 0.6753579712839368 1107 44-45 0.6423758593709259 1107 46-47 0.7263165084422525 1107 48-49 0.8189959307825809 1107 50-51 0.9084179595357895 1107 52-53 1.046571762622893 1107 54-55 0.9229384269719887 1107 56-57 0.9367642562667484 1107 58-59 0.8935700677518916 1107 60-61 0.8900527946290389 1107 62-63 0.7015514821404203 1107 64-65 0.8860874081627728 1107 66-67 0.8524646508347153 1107 68-69 0.7624930646288561 1107 70-71 0.8589108398046221 1107 72-73 0.7408077492267502 1107 74-75 0.7711891889757148 1107 76-77 0.7586594761107541 1107 78-79 0.5693602347341447 1107 80-81 0.4374542516555131 1107 82-83 0.6314180538497105 1107 84-85 0.5326957167165212 1107 86-87 0.5472346884378538 1107 88-89 0.5397017268488682 1107 90-91 0.6641746275340275 1107 92-93 0.7644452448940484 1107 94-95 0.9921063952042495 1107 96-97 2.3544435064300497 1107 98-99 2.6826424230428216 1107 100-101 2.2275847558629245 1108 1 0.4649284800706006 1108 2 0.4461595395204476 1108 3 0.5143065933352808 1108 4 0.5139778252170188 1108 5 0.5282911038271152 1108 6 0.594552757912254 1108 7 0.46316158403782026 1108 8 0.6137204974435733 1108 9 0.4531231354552645 1108 10-11 0.5998494825226999 1108 12-13 0.4015572872238309 1108 14-15 0.44554266571431356 1108 16-17 0.6151286885579239 1108 18-19 0.6744238818279413 1108 20-21 0.48383126637799023 1108 22-23 0.3722903312711665 1108 24-25 0.3749789336420477 1108 26-27 0.49787245479159026 1108 28-29 0.5592219154008831 1108 30-31 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0.845841772298261 2312 64-65 0.7650474929818003 2312 66-67 0.6625989499251581 2312 68-69 0.6812685777863834 2312 70-71 0.8971908700853177 2312 72-73 0.8387544127509479 2312 74-75 1.063738855967312 2312 76-77 0.9208286600239468 2312 78-79 1.0808856265173539 2312 80-81 1.0833664266109508 2312 82-83 1.0499884624978328 2312 84-85 1.250210211859656 2312 86-87 1.2739209989652167 2312 88-89 1.2587467960882215 2312 90-91 1.4514515322321877 2312 92-93 1.3245700683456718 2312 94-95 1.9650454351463083 2312 96-97 3.508163607774593 2312 98-99 4.040632746865024 2312 100-101 3.5943922477509176 2313 1 -0.05652128862975658 2313 2 -0.4013510279429866 2313 3 -0.6250779376439084 2313 4 0.037089085955933854 2313 5 -0.2799764556187654 2313 6 0.0020609562688420624 2313 7 0.04628606189913853 2313 8 0.34665357640964345 2313 9 0.43581930373666466 2313 10-11 -0.5685285521161347 2313 12-13 0.04564730081501267 2313 14-15 0.19149336335393485 2313 16-17 -0.4764513186740871 2313 18-19 0.14876271398295415 2313 20-21 -2.164700822550799 2313 22-23 -0.38787033157999673 2313 24-25 0.1766394905068509 2313 26-27 0.2907034127835786 2313 28-29 0.021186840150569708 2313 30-31 0.24526267472919727 2313 32-33 0.5533942630847903 2313 34-35 0.251915559266088 2313 36-37 -0.5137087172557173 2313 38-39 0.3767578460316301 2313 40-41 0.5516161707025162 2313 42-43 0.5117964125197645 2313 44-45 0.5395320980502198 2313 46-47 0.5288778528061115 2313 48-49 0.21259792988062642 2313 50-51 0.622485576956926 2313 52-53 0.4896373912963483 2313 54-55 0.4673821321050262 2313 56-57 0.5118154535815727 2313 58-59 0.5476314749572175 2313 60-61 0.18715802082136435 2313 62-63 0.360290882263147 2313 64-65 0.12767404436981167 2313 66-67 0.3734352188476677 2313 68-69 0.302177452589909 2313 70-71 0.4017336046814144 2313 72-73 0.21205223211538637 2313 74-75 0.18601681852155494 2313 76-77 0.2361659266027658 2313 78-79 0.31816439853598055 2313 80-81 0.4739577522950178 2313 82-83 0.6923225274230269 2313 84-85 0.8235389753054712 2313 86-87 0.8417264488122385 2313 88-89 0.8377440302474781 2313 90-91 1.0842846370711463 2313 92-93 1.1012625832251288 2313 94-95 1.5801861077599746 2313 96-97 2.8481205456357355 2313 98-99 3.0437479893183923 2313 100-101 2.7034887488838244 2314 1 -0.16162797954880403 2314 2 -0.2942902461110357 2314 3 -1.340155765994048 2314 4 -0.4440636286063935 2314 5 -0.5039889673887146 2314 6 -0.45753074874620125 2314 7 -0.23434863521153204 2314 8 -1.8837052778050456 2314 9 -1.5764315715004855 2314 10-11 -0.7678098517978995 2314 12-13 -0.4824303137900543 2314 14-15 -0.5596338624586465 2314 16-17 -0.6835950838842066 2314 18-19 -1.9573035595604296 2314 20-21 -1.3612476173527384 2314 22-23 -1.8863145678674798 2314 24-25 -1.6128623088633702 2314 26-27 -1.7619578529783695 2314 28-29 -2.905756703211736 2314 30-31 -1.3820998718267177 2314 32-33 -1.1899461112624508 2314 34-35 -1.5115571903336686 2314 36-37 -3.0114874450115536 2314 38-39 -3.5807811030691 2314 40-41 -4.085564329321233 2314 42-43 -2.8926218237062464 2314 44-45 -3.4661496090044857 2314 46-47 -3.5663593692903746 2314 48-49 -1.8829693397427114 2314 50-51 -1.1721267202297447 2314 52-53 -2.639628603959352 2314 54-55 -2.0420725944549645 2314 56-57 -1.6198574438235909 2314 58-59 -3.596164819010699 2314 60-61 -2.174554859484193 2314 62-63 -2.1412793756000177 2314 64-65 -1.6053063302009534 2314 66-67 -2.6545554308535095 2314 68-69 -2.4627401717384743 2314 70-71 -2.038120210458441 2314 72-73 -1.295463421567316 2314 74-75 -1.7091450689172198 2314 76-77 -0.6850246613498392 2314 78-79 -0.43879977890874855 2314 80-81 -0.2132385690546208 2314 82-83 -0.12158200816343268 2314 84-85 -0.14182856142934241 2314 86-87 0.16375905960343218 2314 88-89 0.5321617565587999 2314 90-91 0.89893780427008 2314 92-93 1.0083131667631235 2314 94-95 1.5224235994825897 2314 96-97 3.1444855872810944 2314 98-99 3.2985495696348455 2314 100-101 3.070502995760773 2315 1 -2.3957543924864453 2315 2 -2.023261288827264 2315 3 -1.643893965054204 2315 4 -1.776416196236891 2315 5 -2.267341492684004 2315 6 -3.3220404364534666 2315 7 -2.4251647169467105 2315 8 -1.417574504340756 2315 9 -1.1475769506122901 2315 10-11 -3.8721869597385705 2315 12-13 -2.053483437422983 2315 14-15 -1.4369268432807942 2315 16-17 -2.3023393509911685 2315 18-19 -1.4459741281223089 2315 20-21 -1.2240591339410898 2315 22-23 -1.3124877823799892 2315 24-25 -3.103681036549183 2315 26-27 -4.286760288664134 2315 28-29 -6.247795214141277 2315 30-31 -8.72533417604754 2315 32-33 -7.31404966318658 2315 34-35 -6.699443851920108 2315 36-37 -4.056004329704557 2315 38-39 -6.7693172573386065 2315 40-41 -4.159619724943774 2315 42-43 -4.0689719641180275 2315 44-45 -3.3955260113583208 2315 46-47 -2.5258433383574506 2315 48-49 -3.3231248158021494 2315 50-51 -3.206860996227089 2315 52-53 -2.2976837596823643 2315 54-55 -3.6694906405438985 2315 56-57 -3.683348616932264 2315 58-59 -2.344152838146215 2315 60-61 -2.5107772181198733 2315 62-63 -3.26843176824498 2315 64-65 -2.4991059858745786 2315 66-67 -2.9531232659719784 2315 68-69 -2.1582425848454534 2315 70-71 -2.728673108303582 2315 72-73 -1.8439517017699565 2315 74-75 -2.5290577878255327 2315 76-77 -1.6443634559252835 2315 78-79 -1.6716667193431043 2315 80-81 -1.3158241920172422 2315 82-83 -1.1808064976713588 2315 84-85 -1.0306423641190392 2315 86-87 -1.5333266903648166 2315 88-89 -2.032270140199735 2315 90-91 -1.6347851893937495 2315 92-93 -0.9962142311737381 2315 94-95 -0.2618033595792575 2315 96-97 0.9347712441118841 2315 98-99 0.8858680574211384 2315 100-101 0.7179515937597287 2316 1 -3.694810818328847 2316 2 -2.2529852219849253 2316 3 -2.3415482321541283 2316 4 -3.1253796657445214 2316 5 -2.733085601407886 2316 6 -6.153846052632719 2316 7 -3.7233007403555405 2316 8 -4.843177642762168 2316 9 -6.669264606549909 2316 10-11 -5.865173741078323 2316 12-13 -3.886829164934287 2316 14-15 -5.1754360931097025 2316 16-17 -4.541682244007852 2316 18-19 -2.8448459884884194 2316 20-21 -3.5891999695242376 2316 22-23 -4.422361092326046 2316 24-25 -3.055900672160355 2316 26-27 -4.097431675895976 2316 28-29 -4.035156924753387 2316 30-31 -3.5523755779907304 2316 32-33 -3.540189792840465 2316 34-35 -5.530056932627758 2316 36-37 -5.815305621393449 2316 38-39 -5.596739914973689 2316 40-41 -4.9238221057036995 2316 42-43 -4.0894336741912625 2316 44-45 -3.7829623564769044 2316 46-47 -4.02015036908962 2316 48-49 -4.485861180859185 2316 50-51 -5.569738277698871 2316 52-53 -3.4753885195855823 2316 54-55 -3.585131015386949 2316 56-57 -2.850949696842381 2316 58-59 -3.426662497521871 2316 60-61 -3.731802054974768 2316 62-63 -2.8648097044526857 2316 64-65 -3.3028298974771424 2316 66-67 -4.020863106787623 2316 68-69 -3.1349730545847976 2316 70-71 -2.606432564253055 2316 72-73 -2.6759820297845742 2316 74-75 -3.8597953491901897 2316 76-77 -3.9802995666322367 2316 78-79 -3.7398654141822796 2316 80-81 -3.899352598934268 2316 82-83 -2.9523526983169326 2316 84-85 -2.8761548633780833 2316 86-87 -2.5672581775045096 2316 88-89 -2.6810673660593807 2316 90-91 -2.245692604884315 2316 92-93 -2.0404094448655314 2316 94-95 -2.1643980537933345 2316 96-97 -0.3845884618766604 2316 98-99 0.16780974011940408 2316 100-101 -0.09793450913350377 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23330.0 3 7507.0 4 7276.0 5 7967.0 6 8815.0 7 10569.0 8 12832.0 9 15145.0 10 18000.0 11 20543.0 12 23266.0 13 25654.0 14 27866.0 15 30728.0 16 33029.0 17 35487.0 18 34615.0 19 34757.0 20 37601.0 21 41501.0 22 46081.0 23 50706.0 24 56481.0 25 63994.0 26 71064.0 27 78531.0 28 88898.0 29 99673.0 30 111308.0 31 126906.0 32 144198.0 33 164049.0 34 184968.0 35 202223.0 36 196853.0 37 140764.0 38 31732.0 39 280.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.63119395788422 19.04594075076705 13.767608603052626 23.555256688296105 2 15.647524867215576 19.994136426670956 40.518805162884966 23.8395335432285 3 19.556875449496975 24.255862218987357 29.53461629446263 26.65264603705304 4 12.95828757468266 16.52951687083063 37.28512177387996 33.22707378060675 5 13.872320106709157 37.15102295380046 34.630996605219806 14.345660334270585 6 32.0856112245251 36.0619009722847 17.75098612504758 14.101501678142625 7 28.414408273043662 32.37324029561909 21.083752109742054 18.128599321595193 8 26.01078411950597 35.49900991298134 19.897894302479585 18.5923116650331 9 26.27180341016301 16.755193172925814 20.24594493231713 36.727058484594046 10-11 24.821186300830057 26.621770123151165 27.747262075750694 20.809781500268077 12-13 25.70482148155902 23.91128882260273 27.975242466894652 22.408647228943597 14-15 22.72489200443573 25.489569799648116 26.026757987865746 25.758780208050407 16-17 22.31941738838583 28.566411426633774 26.747069295419866 22.367101889560534 18-19 22.221823276179986 27.4603837567725 28.741483613519627 21.576309353527886 20-21 22.933030021233865 26.643158103811842 28.856768116399838 21.56704375855445 22-23 22.525323479206268 26.618153441846452 28.719729548185157 22.13679353076212 24-25 22.327661775750897 26.77671407514507 28.859967143781667 22.035657005322363 26-27 22.228946565282044 26.89318034731376 28.76541927216923 22.112453815234968 28-29 22.121014034753546 27.16770275067317 28.73420981999844 21.977073394574845 30-31 22.34851182288412 26.89389848140068 28.724620296057495 22.032969399657702 32-33 22.076619972934434 26.93883320216144 28.732987697547905 22.251559127356224 34-35 22.471449369696256 27.037407947262913 28.596959619705775 21.894183063335053 36-37 22.40259220426071 26.830703150118417 28.652719317642383 22.11398532797849 38-39 22.19586291902438 27.05479400179305 28.650187040931748 22.09915603825083 40-41 22.492628240912634 26.87424865179246 28.676683777283294 21.956439330011605 42-43 22.19615384865048 26.83687730263521 28.622965994295924 22.344002854418388 44-45 22.277568474217073 27.026875558135817 28.48515312747557 22.210402840171543 46-47 22.369023922597897 27.11711842349316 28.21742417339636 22.296433480512583 48-49 22.228689767854558 27.150277932491377 28.22323870200472 22.397793597649343 50-51 22.189095527203374 27.330591450426038 28.172489649742033 22.307823372628555 52-53 22.22584810459345 27.359527313901825 27.97903349759519 22.435591083909536 54-55 22.1218315944874 27.317409267883175 28.094557881017945 22.46620125661148 56-57 22.078464648913556 27.42379309299818 28.00461505825104 22.493127199837218 58-59 22.110963153697835 27.256562195072327 28.042133807566668 22.590340843663174 60-61 22.132838886447097 27.269477231919787 28.078298676822218 22.519385204810902 62-63 21.962964958507225 27.22199105164293 28.185485502575297 22.629558487274544 64-65 21.940843458345817 27.20346271640625 28.081691027639632 22.774002797608293 66-67 21.922837765972407 27.241312314447292 27.909204712589915 22.92664520699039 68-69 21.97489390916465 27.18474204667629 27.97279903032338 22.867565013835677 70-71 21.85841134973367 27.014731570714805 27.977477788397064 23.14937929115446 72-73 21.8711815597516 27.065548217897074 27.915294237844467 23.14797598450686 74-75 21.813388937103937 27.22150795642072 27.83761182036178 23.127491286113568 76-77 21.766788889718867 27.06949392727891 27.86720232624353 23.296514856758694 78-79 21.851295385021725 26.979253489323657 27.83782086947862 23.331630256176002 80-81 21.79645033085054 27.078540927801768 27.755592135103672 23.369416606244016 82-83 21.853095984079726 26.9928703429475 27.814798649755705 23.339235023217068 84-85 21.817806348379655 26.948587419829177 27.751978659933712 23.48162757185745 86-87 21.745579580324463 26.846256125147267 27.8718415408521 23.53632275367617 88-89 21.84486140833864 26.735628984433152 27.68281287641956 23.73669673080865 90-91 21.812168716633565 26.779990614539845 27.75141604428829 23.656424624538303 92-93 21.91508664272666 26.604364054660106 27.720455632226404 23.76009367038683 94-95 21.73249486456279 26.52520158038764 27.77392083297885 23.96838272207072 96-97 21.84833900153951 26.339620749347976 27.70862321838216 24.10341703073036 98-99 21.93128437129807 26.2270996099144 27.494114275651395 24.34750174313614 100-101 21.90437279864789 26.343286475858807 27.63326608461467 24.11907464087863 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2831.0 1 1628.5 2 389.0 3 370.0 4 349.5 5 331.0 6 454.0 7 813.5 8 1251.5 9 1429.0 10 1380.0 11 1302.5 12 1327.0 13 1447.5 14 1592.5 15 1776.5 16 2010.5 17 2290.0 18 2583.0 19 2901.5 20 3379.0 21 4158.0 22 5147.0 23 6243.5 24 7697.0 25 9724.0 26 12583.5 27 15969.5 28 19190.0 29 22572.5 30 26369.5 31 30626.0 32 35325.0 33 40226.5 34 44742.5 35 49218.0 36 54838.0 37 60038.0 38 64473.0 39 68588.5 40 72076.5 41 75292.5 42 78358.0 43 82073.0 44 85637.0 45 88295.0 46 90129.5 47 91444.0 48 92836.5 49 92655.5 50 90205.0 51 87177.0 52 83966.5 53 80387.0 54 76486.5 55 71071.5 56 63998.5 57 56695.5 58 50184.0 59 43311.0 60 35746.0 61 29315.5 62 23542.5 63 17876.5 64 13159.0 65 9694.0 66 7139.5 67 5240.0 68 3764.0 69 2689.5 70 1962.0 71 1350.5 72 943.0 73 697.0 74 510.0 75 370.0 76 244.0 77 157.0 78 102.0 79 70.5 80 50.0 81 34.0 82 25.0 83 21.5 84 17.5 85 12.5 86 9.0 87 5.5 88 2.5 89 1.0 90 3.0 91 3.0 92 2.5 93 2.5 94 0.5 95 0.0 96 1.5 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.11929870330688921 2 0.11290615874156712 3 0.12482739049851913 4 0.14029043748760905 5 0.1346753645586099 6 0.1346321716899253 7 0.14266604526526253 8 0.1416294164168319 9 0.14944732564874608 10-11 0.14527921382068135 12-13 0.14404821706317 14-15 0.13238614251832567 16-17 0.13497771463940217 18-19 0.1533562802647032 20-21 0.1842607778085407 22-23 0.20270413273686863 24-25 0.22166580208941183 26-27 0.22382544552364225 28-29 0.2253371959276036 30-31 0.22488367080641516 32-33 0.22680575346288026 34-35 0.1971754455452387 36-37 0.1843471635459099 38-39 0.19896794959564998 40-41 0.18793217164673245 42-43 0.1897678685658283 44-45 0.19400076969691996 46-47 0.21490611814027055 48-49 0.23209687987674482 50-51 0.2467824552295118 52-53 0.26695352490522406 54-55 0.2613384519762249 56-57 0.23181612623029488 58-59 0.22559635313971121 60-61 0.2045830225246491 62-63 0.19408715543428917 64-65 0.18549177456605204 66-67 0.1812372770006181 68-69 0.1867443677579057 70-71 0.19054534020215128 72-73 0.22769120727091477 74-75 0.23848942444206692 76-77 0.254600364461426 78-79 0.24412609380540834 80-81 0.2492444487445345 82-83 0.2457242299467389 84-85 0.23166495118989872 86-87 0.2224648701600771 88-89 0.22423577777614606 90-91 0.22637382477603418 92-93 0.23304712298780622 94-95 0.23801430288653622 96-97 8.690945090201827 98-99 0.29619509700470414 100-101 0.30660457835769483 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2315197.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.90350837636845 #Duplication Level Percentage of deduplicated Percentage of total 1 82.7850071799152 58.6974745001884 2 10.870411062863784 15.415005637006612 3 3.1664040270959286 6.735274633744889 4 1.2191517817099358 3.457685542661398 5 0.611201679105971 2.1668171687070337 6 0.33175846097432526 1.4113703289974506 7 0.2129593366765495 1.0569694878310116 8 0.14486215926322454 0.821698825819109 9 0.10666830466159215 0.6806841329759599 >10 0.5159535549927429 6.318144194064482 >50 0.02484693041437562 1.1811753369486422 >100 0.010283931198436347 1.2898763299954965 >500 1.8434667296447784E-4 0.07729215031530744 >1k 2.457955639510288E-4 0.45787103024736264 >5k 6.14488909877572E-5 0.23266070049680435 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5340 0.23064991877581043 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4291 0.18534059952565593 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2817 0.1216743110845427 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.31928686846087E-5 2 4.31928686846087E-5 0.0 0.0 0.0 4.31928686846087E-5 3 4.31928686846087E-5 0.0 0.0 0.0 4.31928686846087E-5 4 4.31928686846087E-5 0.0 0.0 0.0 4.31928686846087E-5 5 4.31928686846087E-5 0.0 0.0 0.0 4.31928686846087E-5 6 4.31928686846087E-5 0.0 0.0 0.0 4.31928686846087E-5 7 4.31928686846087E-5 0.0 0.0 0.0 4.31928686846087E-5 8 4.31928686846087E-5 0.0 0.0 0.0 4.31928686846087E-5 9 4.31928686846087E-5 0.0 0.0 1.727714747384348E-4 4.31928686846087E-5 10-11 4.31928686846087E-5 0.0 0.0 1.727714747384348E-4 4.31928686846087E-5 12-13 8.63857373692174E-5 0.0 0.0 1.727714747384348E-4 1.295786060538261E-4 14-15 8.63857373692174E-5 0.0 0.0 1.727714747384348E-4 1.295786060538261E-4 16-17 8.63857373692174E-5 0.0 0.0 2.159643434230435E-4 1.295786060538261E-4 18-19 8.63857373692174E-5 0.0 0.0 3.0235008079226085E-4 1.727714747384348E-4 20-21 8.63857373692174E-5 0.0 0.0 5.183144242153043E-4 2.1596434342304347E-4 22-23 1.727714747384348E-4 0.0 0.0 6.910858989537391E-4 2.1596434342304347E-4 24-25 2.591572121076522E-4 0.0 0.0 0.0014469611009343914 2.1596434342304347E-4 26-27 2.591572121076522E-4 0.0 0.0 0.0023324149089688695 2.1596434342304347E-4 28-29 2.591572121076522E-4 0.0 0.0 0.007299594807698869 2.1596434342304347E-4 30-31 2.591572121076522E-4 0.0 0.0 0.021877187988754303 2.3756077776534784E-4 32-33 2.591572121076522E-4 0.0 0.0 0.05897986218883317 2.591572121076522E-4 34-35 2.807536464499565E-4 0.0 0.0 0.11653435971107426 2.591572121076522E-4 36-37 3.0235008079226085E-4 0.0 0.0 0.2159427469887012 2.591572121076522E-4 38-39 3.0235008079226085E-4 0.0 0.0 0.3611355750720133 3.0235008079226085E-4 40-41 3.0235008079226085E-4 0.0 0.0 0.5162195700841008 3.0235008079226085E-4 42-43 3.0235008079226085E-4 0.0 0.0 0.6693166931366963 3.0235008079226085E-4 44-45 3.455429494768696E-4 0.0 0.0 0.8597108582984515 3.6713938381917387E-4 46-47 4.3192868684608694E-4 0.0 0.0 1.088244326508716 4.3192868684608694E-4 48-49 4.3192868684608694E-4 0.0 0.0 1.3295628838496247 4.3192868684608694E-4 50-51 4.3192868684608694E-4 0.0 0.0 1.5752223244933368 4.3192868684608694E-4 52-53 4.3192868684608694E-4 0.0 0.0 1.811876052016308 4.3192868684608694E-4 54-55 4.3192868684608694E-4 0.0 0.0 2.0737328184167483 4.3192868684608694E-4 56-57 4.3192868684608694E-4 0.0 0.0 2.3809852898047117 4.3192868684608694E-4 58-59 4.3192868684608694E-4 0.0 0.0 2.7055796979695463 4.3192868684608694E-4 60-61 4.3192868684608694E-4 0.0 0.0 3.041900969982252 4.3192868684608694E-4 62-63 4.3192868684608694E-4 0.0 0.0 3.374486058853739 4.3192868684608694E-4 64-65 4.3192868684608694E-4 0.0 0.0 3.711390434593687 4.3192868684608694E-4 66-67 4.3192868684608694E-4 0.0 2.159643434230435E-5 4.095850158755389 4.3192868684608694E-4 68-69 4.3192868684608694E-4 0.0 4.31928686846087E-5 4.514151495531482 4.3192868684608694E-4 70-71 4.3192868684608694E-4 0.0 4.31928686846087E-5 4.950334679943003 4.3192868684608694E-4 72-73 4.3192868684608694E-4 0.0 4.31928686846087E-5 5.380362880566967 4.7512155553069567E-4 74-75 4.3192868684608694E-4 0.0 4.31928686846087E-5 5.804711218958905 4.7512155553069567E-4 76-77 4.3192868684608694E-4 0.0 4.31928686846087E-5 6.258754654571511 4.7512155553069567E-4 78-79 4.3192868684608694E-4 0.0 4.31928686846087E-5 6.760159070696792 4.7512155553069567E-4 80-81 4.7512155553069567E-4 0.0 4.31928686846087E-5 7.288213486800475 4.7512155553069567E-4 82-83 4.7512155553069567E-4 0.0 4.31928686846087E-5 7.811171144399375 4.7512155553069567E-4 84-85 4.96717989873E-4 0.0 4.31928686846087E-5 8.318125844150629 4.7512155553069567E-4 86-87 5.183144242153043E-4 0.0 4.31928686846087E-5 8.771046265177434 5.183144242153043E-4 88-89 5.183144242153043E-4 0.0 4.31928686846087E-5 9.23606068943593 5.61507292899913E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4225 0.0 42.19154 1 GTATCAA 6780 0.0 36.212097 1 ATCAACG 8260 0.0 29.327675 3 TATCAAC 8310 0.0 29.206554 2 TCAACGC 8440 0.0 28.758556 4 CAACGCA 8600 0.0 28.224737 5 AACGCAG 8960 0.0 27.196884 6 TACATGG 7375 0.0 25.064682 2 GTACATG 7610 0.0 24.90768 1 ACGCAGA 10055 0.0 23.814043 7 ACATGGG 7725 0.0 23.564756 3 CGCAGAG 10115 0.0 23.347729 8 GCAGAGT 10755 0.0 20.515781 9 GAGTACT 5755 0.0 19.536646 12-13 CATGGGA 4200 0.0 19.039675 4 AGAGTAC 9165 0.0 18.8635 10-11 CAGAGTA 10305 0.0 18.214712 10-11 GTACTTT 6210 0.0 18.110897 14-15 AGTACTT 6365 0.0 17.479546 12-13 GAGTACA 5655 0.0 17.133556 1 >>END_MODULE