FastQCFastQC Report
Fri 27 May 2016
SRR939798_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939798_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2315197
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59140.25544262540077584No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55910.24149132881564722No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34640.1496200971234845No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46150.043.8033071
GTATCAA73550.035.5498621
ATCAACG89750.028.9115773
TCAACGC90150.028.7832934
TATCAAC91400.028.7066352
CAACGCA91450.028.4260035
AACGCAG95150.027.320636
ACGCAGA109000.023.8926727
GTACATG74100.023.5026511
CGCAGAG110950.023.472758
TACATGG74650.023.1352862
ACATGGG76750.021.2617473
GAGTACT66200.021.21104212-13
GCAGAGT125900.019.9695859
GTACTTT71800.019.2263514-15
AGAGTAC103700.018.98422810-11
AGTACTT72700.018.3684412-13
CAGAGTA118950.018.36490810-11
TGCACCG3501.3369572E-917.6195285
TGGTATC26900.017.2853092
Produced by FastQC (version 0.11.5)