##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939798_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2315197 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.35138392110909 31.0 31.0 34.0 30.0 34.0 2 31.578381882837615 31.0 31.0 34.0 30.0 34.0 3 31.62518394762951 31.0 31.0 34.0 30.0 34.0 4 35.44010941617495 37.0 35.0 37.0 33.0 37.0 5 35.167876426930405 37.0 35.0 37.0 32.0 37.0 6 35.186520196769436 37.0 35.0 37.0 32.0 37.0 7 35.091650084204495 37.0 35.0 37.0 32.0 37.0 8 35.05132090271368 37.0 35.0 37.0 32.0 37.0 9 36.63531224340736 39.0 35.0 39.0 32.0 39.0 10-11 36.495991917750416 38.0 35.0 39.0 32.0 39.0 12-13 36.41886306003333 38.0 35.0 39.0 32.0 39.0 14-15 37.45022129866271 39.0 36.0 41.0 32.0 41.0 16-17 37.36195710343439 39.0 36.0 41.0 32.0 41.0 18-19 37.32990561926264 39.0 36.0 41.0 32.0 41.0 20-21 37.23577475264524 39.0 36.0 40.0 31.0 41.0 22-23 37.11031739415695 39.0 36.0 40.0 31.0 41.0 24-25 36.97141863089836 39.0 36.0 40.0 31.0 41.0 26-27 36.67151952943961 38.5 35.5 40.0 30.0 41.0 28-29 36.443442393887 38.0 35.0 40.0 30.0 41.0 30-31 36.18880423566547 38.0 35.0 40.0 30.0 41.0 32-33 36.188429537529636 38.0 35.0 40.0 30.0 41.0 34-35 36.057255386906604 38.0 35.0 40.0 29.5 41.0 36-37 36.12000879406807 38.0 35.0 40.0 30.0 41.0 38-39 36.03304103279332 38.0 35.0 40.0 29.0 41.0 40-41 35.95615146356876 38.0 35.0 40.0 29.0 41.0 42-43 35.74910774331515 38.0 34.5 40.0 28.5 41.0 44-45 35.52102628847567 38.0 34.0 40.0 27.5 41.0 46-47 35.268729615665535 38.0 34.0 40.0 27.0 41.0 48-49 34.983421281212784 38.0 33.5 40.0 26.0 41.0 50-51 34.77240878422009 38.0 33.0 40.0 26.0 41.0 52-53 34.53612543554608 38.0 33.0 40.0 24.5 41.0 54-55 34.28347047789022 37.5 33.0 40.0 24.0 41.0 56-57 33.91449150979376 37.0 32.5 40.0 23.5 41.0 58-59 33.55160381600356 37.0 32.0 39.5 23.0 41.0 60-61 33.24587799655926 36.0 31.5 39.0 22.0 41.0 62-63 32.95163608107647 36.0 31.0 39.0 22.0 40.0 64-65 32.51545246473626 35.5 31.0 39.0 20.5 40.0 66-67 31.609457640105788 35.0 30.0 38.5 16.0 40.0 68-69 29.695561328042494 34.0 26.5 37.5 9.5 40.0 70-71 29.039717354505903 33.5 25.5 37.0 9.0 39.0 72-73 29.608140689539596 34.0 27.5 36.0 9.5 39.0 74-75 29.712954232404414 34.0 28.0 36.0 11.0 39.0 76-77 29.279276666305286 32.5 27.5 35.0 10.5 38.0 78-79 29.81354308078319 34.0 29.0 35.5 10.5 37.5 80-81 29.623113065540426 34.0 29.0 35.0 7.5 37.0 82-83 29.170579004724004 34.0 29.0 35.0 7.0 36.5 84-85 28.803713031763603 33.0 28.5 35.0 3.5 36.0 86-87 28.41066267794922 33.0 27.5 35.0 2.0 36.0 88-89 27.9493086333474 33.0 26.5 35.0 2.0 35.0 90-91 27.453491862679506 33.0 26.0 35.0 2.0 35.0 92-93 26.952903575808023 32.0 25.5 34.0 2.0 35.0 94-95 26.127885877530076 32.0 24.0 34.0 2.0 35.0 96-97 25.591603219941973 32.0 23.0 34.0 2.0 35.0 98-99 24.90340865161798 31.5 19.0 34.0 2.0 35.0 100-101 23.40768604140382 30.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.25422713151721865 1101 2 0.38534708924324335 1101 3 0.3280668645975986 1101 4 0.22194240904654805 1101 5 0.30657739299332576 1101 6 0.20699391245575072 1101 7 0.16022190784264723 1101 8 0.18603160694836873 1101 9 0.3737692756892912 1101 10-11 0.44473791236888616 1101 12-13 0.36826811284758776 1101 14-15 0.3462628508682215 1101 16-17 0.3074381540929281 1101 18-19 0.33663846200864356 1101 20-21 0.29434767027890985 1101 22-23 0.42051216937510105 1101 24-25 0.23781151377506404 1101 26-27 0.2623814737025043 1101 28-29 0.3565542108994606 1101 30-31 0.18123523473663994 1101 32-33 0.06258402019729914 1101 34-35 0.031430702798296295 1101 36-37 0.12368194889801742 1101 38-39 0.18663193594674254 1101 40-41 0.19917927313401407 1101 42-43 0.08421221102084075 1101 44-45 0.13724897331740493 1101 46-47 0.04608191731191624 1101 48-49 -0.14667729158968967 1101 50-51 -0.19780545507075686 1101 52-53 -0.22613637841399736 1101 54-55 -0.38914657486856896 1101 56-57 -0.5846314369743482 1101 58-59 -0.38428835256373617 1101 60-61 -0.6232370793232604 1101 62-63 -0.678474638178777 1101 64-65 -0.6297040242608603 1101 66-67 -0.8117880811705618 1101 68-69 -9.314268650223926 1101 70-71 -7.395051004205325 1101 72-73 -2.2010695423769278 1101 74-75 -1.1052506581031558 1101 76-77 -0.949271975956087 1101 78-79 -0.9688105872748878 1101 80-81 -0.899490155276446 1101 82-83 -0.789033239679803 1101 84-85 -0.6123623890852592 1101 86-87 -0.7462217759444876 1101 88-89 -0.6166921261959253 1101 90-91 -0.9061753532401262 1101 92-93 -1.1758108804544847 1101 94-95 -0.8051135043132511 1101 96-97 -0.986536909411754 1101 98-99 -0.6883993181526513 1101 100-101 -0.5312980583355191 1102 1 0.21364319801459075 1102 2 0.14291199772609886 1102 3 0.17679089773526968 1102 4 0.17744523296450865 1102 5 0.22070253349539115 1102 6 0.18677826781147644 1102 7 0.28830150486191997 1102 8 0.2199186224764489 1102 9 0.2611095196424955 1102 10-11 0.3410120058704109 1102 12-13 0.20681077690542793 1102 14-15 0.367906412751573 1102 16-17 0.3655026254582694 1102 18-19 0.28605216945907586 1102 20-21 0.12720086817519416 1102 22-23 0.20245990954689574 1102 24-25 0.10705509231051025 1102 26-27 0.274165607030298 1102 28-29 0.18364439587649883 1102 30-31 0.22421429472581167 1102 32-33 0.1682776493828797 1102 34-35 0.06009034783111389 1102 36-37 0.03103940001717831 1102 38-39 0.42362040437020454 1102 40-41 0.3902121193935386 1102 42-43 0.23272809295748687 1102 44-45 0.30539212583672537 1102 46-47 0.3085022994775173 1102 48-49 0.3508054665524867 1102 50-51 0.3043385022998777 1102 52-53 0.32218245371900167 1102 54-55 0.0634088280427747 1102 56-57 0.13309749465491905 1102 58-59 0.1200328663696979 1102 60-61 0.33272784026099345 1102 62-63 -0.02589148177396794 1102 64-65 -0.021283614341761847 1102 66-67 0.4435967904001501 1102 68-69 -4.529442872779189 1102 70-71 0.4180833365798229 1102 72-73 0.6096890993671025 1102 74-75 0.1878664755160422 1102 76-77 -0.008012244887435571 1102 78-79 0.039237925831702825 1102 80-81 0.05165537016656785 1102 82-83 0.0595894932066976 1102 84-85 -0.05808904492806377 1102 86-87 -0.06986914005644351 1102 88-89 -0.18707637331302962 1102 90-91 -0.347873589505614 1102 92-93 -0.45548838486035237 1102 94-95 -0.04786776100531398 1102 96-97 -0.6747749788787516 1102 98-99 -0.5003083422010555 1102 100-101 -0.4902548045159456 1103 1 0.20279048064483618 1103 2 0.28437076587103505 1103 3 0.20616145274411934 1103 4 0.34738636893573016 1103 5 0.2201147943715327 1103 6 0.2621716258727531 1103 7 0.13024365734607102 1103 8 0.2070703676705392 1103 9 0.31124000818149256 1103 10-11 0.32771226792888086 1103 12-13 0.34300882029826596 1103 14-15 0.3575864635051218 1103 16-17 0.3501217395059655 1103 18-19 0.20174900348990832 1103 20-21 0.3014927335485069 1103 22-23 0.22885401016726092 1103 24-25 0.3712416656886859 1103 26-27 0.24108018389777897 1103 28-29 0.1260833600426139 1103 30-31 0.10074681412787356 1103 32-33 0.29122308570843103 1103 34-35 0.2601701008400923 1103 36-37 0.32342681760573555 1103 38-39 0.47203147210529295 1103 40-41 0.554122846488653 1103 42-43 0.3759959477186925 1103 44-45 0.4623063061920689 1103 46-47 0.38232979507083087 1103 48-49 0.3438814635101579 1103 50-51 0.38877837483924793 1103 52-53 0.34580223102336305 1103 54-55 0.3255771546534234 1103 56-57 0.4391153773522092 1103 58-59 0.36084297670821286 1103 60-61 0.28897741220811923 1103 62-63 0.24707242841242305 1103 64-65 0.3721892552165613 1103 66-67 0.4875208727449021 1103 68-69 -3.766110422586525 1103 70-71 0.9631147691781692 1103 72-73 1.1845348201000014 1103 74-75 0.7212436955334134 1103 76-77 0.5491905782789637 1103 78-79 0.40221522731429715 1103 80-81 0.604646873992909 1103 82-83 0.5431639780112718 1103 84-85 0.3874900249986588 1103 86-87 0.30500514757346053 1103 88-89 0.19801819483118166 1103 90-91 -0.16962795744725057 1103 92-93 0.055986856561467846 1103 94-95 -0.028577445633978016 1103 96-97 -0.5484521603263062 1103 98-99 -0.042062241537440315 1103 100-101 0.07599939941384903 1104 1 0.2070622602443457 1104 2 0.21043809524071122 1104 3 0.2195520000557316 1104 4 0.2502285841022456 1104 5 0.22606902318891287 1104 6 0.16115491271125393 1104 7 0.20035575877688672 1104 8 0.18650470076764947 1104 9 0.19409747422385237 1104 10-11 0.35592567456918545 1104 12-13 0.2930580091900339 1104 14-15 0.3876494937977739 1104 16-17 0.4236376337446828 1104 18-19 0.304405167962436 1104 20-21 0.26345947921713275 1104 22-23 0.2383691258048657 1104 24-25 0.16702382080800504 1104 26-27 0.25050769799074146 1104 28-29 0.25710752736405595 1104 30-31 0.3047753611697246 1104 32-33 0.3739635474881098 1104 34-35 0.3269291684028346 1104 36-37 0.28440389775882835 1104 38-39 0.3487382749093939 1104 40-41 0.3051630033221784 1104 42-43 0.3279099254996609 1104 44-45 0.25770629734271466 1104 46-47 0.19395917666458473 1104 48-49 0.42562226855942953 1104 50-51 0.3094112861864815 1104 52-53 0.183282364563091 1104 54-55 0.12521877334442166 1104 56-57 0.19950151282880313 1104 58-59 0.24439893571853588 1104 60-61 0.298333471273331 1104 62-63 0.3585016824040963 1104 64-65 0.29756041100743147 1104 66-67 0.6376461019183921 1104 68-69 -3.9891444012809707 1104 70-71 0.9303814975049036 1104 72-73 1.0550764188799313 1104 74-75 0.765328236198016 1104 76-77 0.8381316950541411 1104 78-79 0.47059081991822893 1104 80-81 0.35024788481839053 1104 82-83 0.47603996348404465 1104 84-85 0.35293362423390207 1104 86-87 0.3502198552126714 1104 88-89 0.24599939850138597 1104 90-91 0.30816719196066344 1104 92-93 0.09804505396075314 1104 94-95 0.37594543460489405 1104 96-97 0.28100368956567934 1104 98-99 0.6600815341854798 1104 100-101 0.8104083762349461 1105 1 0.14568929290168953 1105 2 0.2566670218284379 1105 3 0.28492960969563796 1105 4 0.09964208430449162 1105 5 0.2682742730031933 1105 6 0.3493789167719399 1105 7 0.22099388959131971 1105 8 0.31127516349047823 1105 9 0.3723141024250154 1105 10-11 0.25378958335175383 1105 12-13 0.13573224734834355 1105 14-15 0.21720871348999538 1105 16-17 0.3077998920795366 1105 18-19 0.239281615706183 1105 20-21 0.49803904200579296 1105 22-23 0.3909441996295513 1105 24-25 0.3857582396352157 1105 26-27 0.32889193532950856 1105 28-29 0.35894250374281 1105 30-31 0.43713608564303996 1105 32-33 0.24449712086734365 1105 34-35 0.28362785000098967 1105 36-37 0.1941180898114041 1105 38-39 0.19560961506870456 1105 40-41 0.18254343640938941 1105 42-43 0.17766393133160818 1105 44-45 0.23377546651426684 1105 46-47 0.19576745190311584 1105 48-49 0.3875398334154454 1105 50-51 0.29415409604528264 1105 52-53 0.10580755219807969 1105 54-55 0.2659485868858624 1105 56-57 0.4026337416337782 1105 58-59 0.38416645074123323 1105 60-61 0.30346987180100626 1105 62-63 0.392594544211164 1105 64-65 0.3764784067828302 1105 66-67 1.0534727401390995 1105 68-69 2.3138852528467346 1105 70-71 2.406058873708105 1105 72-73 1.4768151125332558 1105 74-75 0.8183075282741221 1105 76-77 0.5205393447986317 1105 78-79 0.5405946003766964 1105 80-81 0.5344392236029876 1105 82-83 0.614744455884793 1105 84-85 0.49118922023771816 1105 86-87 0.3983131029966067 1105 88-89 0.5259148537029539 1105 90-91 0.6722108020605653 1105 92-93 0.8013544091414389 1105 94-95 0.9762158827874998 1105 96-97 0.4625402476565199 1105 98-99 0.8614670854318405 1105 100-101 0.7071653773532205 1106 1 0.3565716605556375 1106 2 0.42844653678316647 1106 3 0.44149745736977763 1106 4 0.267655290487383 1106 5 0.43165076290528503 1106 6 0.3458509013829172 1106 7 0.3569411740017543 1106 8 0.39498344509505046 1106 9 0.48567717695536317 1106 10-11 0.4063955761574718 1106 12-13 0.44818625990876626 1106 14-15 0.6293771377977393 1106 16-17 0.5611071222827633 1106 18-19 0.6482703695318364 1106 20-21 0.6364929545193831 1106 22-23 0.5780672617904727 1106 24-25 0.6332250081677628 1106 26-27 0.6648219389221026 1106 28-29 0.843813602484488 1106 30-31 0.8730078801650194 1106 32-33 0.8641681486667139 1106 34-35 0.7946261810377919 1106 36-37 0.9376607484197592 1106 38-39 0.990941975219144 1106 40-41 0.7276595589576758 1106 42-43 0.8817722183932801 1106 44-45 1.0941164443356968 1106 46-47 1.0771014400884908 1106 48-49 1.164101587327501 1106 50-51 1.1681055369471665 1106 52-53 1.0699439433266562 1106 54-55 1.074068752236002 1106 56-57 1.0717497368497249 1106 58-59 1.2404532577463314 1106 60-61 1.09489722187854 1106 62-63 1.0764301581576845 1106 64-65 1.1329011865388097 1106 66-67 1.6163703694837395 1106 68-69 3.147753382016301 1106 70-71 3.379817497063584 1106 72-73 2.2713698358810035 1106 74-75 1.7232953180120454 1106 76-77 1.332988809775415 1106 78-79 1.2415259455064565 1106 80-81 1.2009399034667432 1106 82-83 1.2832015062310944 1106 84-85 1.294746340162046 1106 86-87 1.3118267025454884 1106 88-89 1.3987630508134181 1106 90-91 1.2884233925006363 1106 92-93 1.4725882140591935 1106 94-95 2.0066228460475735 1106 96-97 1.8268180686363422 1106 98-99 1.9040774893936963 1106 100-101 1.8528058362953637 1107 1 0.07869080095746384 1107 2 0.0678944838156248 1107 3 0.03289983023222831 1107 4 0.0336878348471501 1107 5 -0.011039919418365685 1107 6 0.06848139830554345 1107 7 -0.07154560742304739 1107 8 0.07163847506434706 1107 9 0.017086661640725254 1107 10-11 0.10902073464234263 1107 12-13 0.07718019292052247 1107 14-15 0.074965659425267 1107 16-17 -0.04443104983248958 1107 18-19 0.038421043281076095 1107 20-21 0.06730597847927555 1107 22-23 -0.0348679374365517 1107 24-25 -0.008568926306573132 1107 26-27 -0.00378914400376118 1107 28-29 0.07174030500279116 1107 30-31 -0.002085291113822052 1107 32-33 0.17285837576056906 1107 34-35 0.08347094859178839 1107 36-37 -0.004752591093001968 1107 38-39 0.10862527605564765 1107 40-41 0.1418455431818586 1107 42-43 0.06530423485102688 1107 44-45 0.045769943160600235 1107 46-47 -0.06955230097717191 1107 48-49 0.014255478765257124 1107 50-51 0.0826470209048793 1107 52-53 0.04707394799778086 1107 54-55 -0.03687166293722299 1107 56-57 0.016833849238466314 1107 58-59 -0.024183248575241123 1107 60-61 0.0719321584800241 1107 62-63 0.20223894518967 1107 64-65 0.06147974521546473 1107 66-67 0.5035346260834359 1107 68-69 1.9650377626066948 1107 70-71 2.211674990027177 1107 72-73 1.2184363917568035 1107 74-75 0.7546431912753384 1107 76-77 0.4853958144639172 1107 78-79 0.4815646857448428 1107 80-81 0.3438018911389733 1107 82-83 0.3440086889894012 1107 84-85 0.2974175700960551 1107 86-87 0.20327404991781606 1107 88-89 0.426741766535212 1107 90-91 0.41067709078566494 1107 92-93 0.29109485922585066 1107 94-95 0.5427616929632997 1107 96-97 0.3800493317710476 1107 98-99 0.5021360897174745 1107 100-101 0.5313247488994683 1108 1 -0.15386638781422945 1108 2 -0.13329824617118646 1108 3 -0.1062810039030353 1108 4 -0.0929797123725038 1108 5 -0.1635962486026159 1108 6 -0.005707287427192398 1108 7 -0.05535706006292429 1108 8 -0.07378694733902336 1108 9 -0.16089563505112636 1108 10-11 -0.037532110067949986 1108 12-13 -0.09281291989718454 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-0.4633618242153901 2316 64-65 -0.5394328199937135 2316 66-67 0.05368158051275884 2316 68-69 1.5176122502844258 2316 70-71 1.714990815843258 2316 72-73 0.8538274100183365 2316 74-75 0.26542271980484244 2316 76-77 -0.13322259324003838 2316 78-79 -0.4001180272368252 2316 80-81 -0.6207958423022149 2316 82-83 -0.46161481381648173 2316 84-85 -0.56647803278846 2316 86-87 -0.6425481225750893 2316 88-89 -0.5055935360574431 2316 90-91 -0.7272606711476222 2316 92-93 -0.7437872408581612 2316 94-95 -0.6358575005068765 2316 96-97 -0.7133868831177139 2316 98-99 -0.767982620183556 2316 100-101 -0.7563550287609822 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 18.0 4 33.0 5 75.0 6 198.0 7 585.0 8 1188.0 9 2169.0 10 3476.0 11 5242.0 12 7401.0 13 9673.0 14 11848.0 15 14497.0 16 17601.0 17 20603.0 18 19755.0 19 17332.0 20 17581.0 21 18388.0 22 20159.0 23 22949.0 24 27888.0 25 34236.0 26 42261.0 27 52361.0 28 64877.0 29 78226.0 30 93704.0 31 110644.0 32 132043.0 33 159801.0 34 196732.0 35 245470.0 36 308244.0 37 341655.0 38 200036.0 39 16243.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.63688921665325 19.971434385275487 14.722567483672625 23.669108914398635 2 15.7600658419146 20.954232524782526 39.51067878952837 23.775022843774504 3 19.346275367733604 24.517675616956673 30.210587013188956 25.92546200212077 4 13.310875921141916 17.353814815758657 37.001257344407406 32.33405191869201 5 13.462612468830946 37.56574494524656 34.820751754602306 14.150890831320186 6 31.92996702221627 36.8021268186913 17.326445504590325 13.941460654502105 7 28.149872343476602 32.43603028165637 21.112415055824624 18.3016823190424 8 25.746232394046814 36.2289688523266 19.636644311477596 18.38815444214898 9 25.660149006758388 16.768594637950894 20.179146742156284 37.39210961313443 10-11 24.610648683459765 26.913735634591784 27.792062619293308 20.683553062655143 12-13 25.453989444526755 24.006682800642885 28.131968899406836 22.407358855423535 14-15 22.27650605974351 25.670277734464932 26.142483771359416 25.910732434432145 16-17 21.950948238738384 28.83503578760593 26.871911426593186 22.342104547062505 18-19 21.88556740527912 27.60551261944448 28.878579231054637 21.630340744221765 20-21 22.56663255869803 26.79437214198187 28.98507124879654 21.653924050523564 22-23 22.25035277775498 26.7384373770353 28.865167845328067 22.146041999881653 24-25 22.09839298668298 26.86138168461368 29.03247263568023 22.007752693023118 26-27 21.980302666761403 27.080194187379718 28.85456528016209 22.08493786569678 28-29 21.877866927897248 27.32632571929115 28.763720220663824 22.032087132147776 30-31 22.151100072888266 26.97593607429204 28.80291553059957 22.070048322220124 32-33 21.94442176773783 26.99134959231601 28.763933756372694 22.300294883573468 34-35 22.249811617249446 27.09446091001233 28.702209688089138 21.95351778464909 36-37 22.25558246618438 26.919346013348754 28.743931326515764 22.081140193951097 38-39 21.97367385708344 27.176039976030985 28.709533909846407 22.14075225703917 40-41 22.275698122083593 26.936309439392314 28.762448244014983 22.02554419450911 42-43 22.017516778697605 26.941300285287582 28.69347539772929 22.347707538285526 44-45 22.0996322806168 27.057185660457307 28.631482918667217 22.211699140258677 46-47 22.19917434012708 27.160029208074675 28.314517701450765 22.326278750347484 48-49 22.045602422790836 27.227690026307428 28.29323810491411 22.43346944598763 50-51 22.029034629585716 27.383055084259393 28.237008420211186 22.3509018659437 52-53 22.17568881237219 27.325437397963526 28.046224980127604 22.452648809536672 54-55 22.02636269987205 27.284726623725046 28.160335453762197 22.528575222640708 56-57 22.009283467210384 27.351316972550993 28.0885156951777 22.550883865060918 58-59 21.998973609318384 27.23995942819326 28.072886697873088 22.68818026461527 60-61 22.013962802720336 27.263491098086917 28.14512952007077 22.577416579121973 62-63 21.90329846900761 27.252485244032282 28.20051702578434 22.64369926117577 64-65 21.77766652786825 27.171324188763545 28.156548204230646 22.89446107913756 66-67 21.59970568152743 27.281644048107488 28.143014380900617 22.975635889464467 68-69 21.55486686286602 27.049793175350352 28.480153247133988 22.91518671464964 70-71 21.476132764141802 26.9603177528353 28.344610928390708 23.218938554632196 72-73 21.469613601517356 27.014468497910865 28.192617922574055 23.323299977997724 74-75 21.591126588048244 27.20248600702943 27.751338810268066 23.455048594654265 76-77 21.46059298893244 27.153002447168127 27.869849022169685 23.516555541729748 78-79 21.53593530448695 27.114117760381774 27.780376667699024 23.56957026743225 80-81 21.475888399146235 27.090226750919967 27.705777761076277 23.728107088857517 82-83 21.468268777243914 27.051662148690653 27.73354826114074 23.746520812924693 84-85 21.384059490594616 26.964056289992676 27.75077374875793 23.90111047065478 86-87 21.33760314032959 26.965027637267397 27.763845521561908 23.933523700841103 88-89 21.33470542946213 26.908734370900046 27.6440943895594 24.11246581007843 90-91 21.299786734335772 26.82668250411684 27.68449639608023 24.189034365467162 92-93 21.267110610641094 26.734040067261095 27.692009656364558 24.30683966573325 94-95 20.903421936869304 26.57682473150187 27.973514250559926 24.546239081068897 96-97 21.009661243053444 26.42313687135392 27.967557680610465 24.59964420498217 98-99 20.98071743164052 26.45157109623572 27.708167281194584 24.859544190929178 100-101 20.837509028140154 26.38882169380004 27.852114744637547 24.92155453342226 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 391.0 1 259.0 2 184.0 3 259.5 4 243.5 5 245.0 6 402.0 7 779.0 8 1237.5 9 1557.0 10 1555.5 11 1433.0 12 1521.5 13 1701.5 14 1862.5 15 1979.0 16 2147.5 17 2407.5 18 2658.0 19 2987.0 20 3432.5 21 4038.0 22 4999.0 23 6157.0 24 7539.0 25 9495.5 26 12389.0 27 16065.0 28 19415.5 29 22672.0 30 26489.0 31 30986.5 32 35990.0 33 40655.5 34 45119.0 35 49480.0 36 54961.5 37 60535.0 38 65057.0 39 69007.0 40 72012.5 41 75259.5 42 78472.5 43 82056.5 44 85930.0 45 88726.0 46 90286.0 47 92047.0 48 93982.5 49 92807.5 50 90144.5 51 87732.5 52 84364.0 53 81310.0 54 77925.5 55 72081.5 56 64529.5 57 57137.5 58 50319.0 59 43005.5 60 35380.0 61 28588.5 62 22521.0 63 16886.0 64 12159.5 65 8589.0 66 6133.5 67 4581.0 68 3223.0 69 2182.0 70 1553.0 71 1049.5 72 699.0 73 520.5 74 363.5 75 229.0 76 134.0 77 74.0 78 43.0 79 18.0 80 13.5 81 12.5 82 6.5 83 4.0 84 3.0 85 1.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022719448928104173 2 0.02427439220075009 3 8.638573736921738E-5 4 0.0 5 0.0 6 8.638573736921738E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.3192868684608694E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 6.478930302691304E-5 26-27 4.5352512118839133E-4 28-29 4.319286868460869E-5 30-31 4.1033225250378265E-4 32-33 0.001986871959492 34-35 0.0041681118280647396 36-37 0.10355490267134936 38-39 0.09549943266166983 40-41 0.10456993508543766 42-43 0.10552017819649906 44-45 0.09899805502512314 46-47 0.09234635324769339 48-49 0.093382982096124 50-51 0.09206559960124344 52-53 0.07282317660225027 54-55 0.06200336299675578 56-57 0.03282658020030261 58-59 0.012482739049851914 60-61 0.008163452181391044 62-63 0.0013821717979074781 64-65 0.003844165312930174 66-67 0.147525242992281 68-69 0.405105915392945 70-71 0.3888222038988475 72-73 0.1759029577180689 74-75 0.027729821695518787 76-77 0.029587115048956954 78-79 0.004578444080568522 80-81 0.001533346838303609 82-83 4.319286868460869E-5 84-85 3.239465151345652E-4 86-87 0.001123014585799826 88-89 1.9436790908073913E-4 90-91 4.1033225250378265E-4 92-93 0.0017277147473843478 94-95 0.0745724877839769 96-97 0.016456482968835912 98-99 0.017795461898058786 100-101 0.009200081029821653 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2315197.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.98836655327872 #Duplication Level Percentage of deduplicated Percentage of total 1 78.4958916249851 51.013197778509735 2 13.173337467306995 17.122273681107746 3 4.021392529260439 7.840311952385822 4 1.6671879669784457 4.333912908448431 5 0.8195875538282086 2.6631828185346333 6 0.4549291898231602 1.773906296640819 7 0.27943822200338353 1.2712163520386655 8 0.20150850795058228 1.0476567022637375 9 0.14749954497818926 0.8627179045935949 >10 0.6868211347640135 7.829644898845178 >50 0.03713459914919663 1.6380683704732633 >100 0.014539396969311476 1.7068668950715293 >500 4.65984910996379E-4 0.18706611233512468 >1k 1.3313854599851833E-4 0.21224476112708723 >5k 1.3313854599851833E-4 0.4977325676247735 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5914 0.25544262540077584 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5591 0.24149132881564722 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3464 0.1496200971234845 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.31928686846087E-5 0.0 4 0.0 0.0 0.0 4.31928686846087E-5 0.0 5 8.63857373692174E-5 0.0 0.0 4.31928686846087E-5 0.0 6 8.63857373692174E-5 0.0 0.0 4.31928686846087E-5 0.0 7 8.63857373692174E-5 0.0 0.0 4.31928686846087E-5 0.0 8 1.295786060538261E-4 0.0 0.0 4.31928686846087E-5 0.0 9 1.295786060538261E-4 0.0 0.0 4.31928686846087E-5 0.0 10-11 1.295786060538261E-4 0.0 0.0 1.5117504039613043E-4 0.0 12-13 1.295786060538261E-4 0.0 0.0 2.591572121076522E-4 6.478930302691304E-5 14-15 1.295786060538261E-4 0.0 0.0 2.591572121076522E-4 8.63857373692174E-5 16-17 1.5117504039613043E-4 0.0 0.0 3.0235008079226085E-4 8.63857373692174E-5 18-19 1.727714747384348E-4 0.0 0.0 3.4554294947686953E-4 8.63857373692174E-5 20-21 2.1596434342304347E-4 0.0 0.0 5.399108585576087E-4 1.295786060538261E-4 22-23 2.1596434342304347E-4 0.0 0.0 7.126823332960434E-4 2.1596434342304347E-4 24-25 2.1596434342304347E-4 0.0 0.0 0.0014469611009343914 2.1596434342304347E-4 26-27 2.1596434342304347E-4 0.0 0.0 0.002375607777653478 2.591572121076522E-4 28-29 2.1596434342304347E-4 0.0 0.0 0.007429173413752696 2.591572121076522E-4 30-31 2.1596434342304347E-4 0.0 0.0 0.022827431099815696 2.591572121076522E-4 32-33 2.1596434342304347E-4 0.0 0.0 0.06176580221899043 2.591572121076522E-4 34-35 2.1596434342304347E-4 0.0 0.0 0.12195506473099266 2.591572121076522E-4 36-37 2.1596434342304347E-4 0.0 0.0 0.22676256059419564 2.591572121076522E-4 38-39 2.1596434342304347E-4 0.0 0.0 0.38171697700022933 2.807536464499565E-4 40-41 2.1596434342304347E-4 0.0 0.0 0.5464329817289846 3.0235008079226085E-4 42-43 2.1596434342304347E-4 0.0 0.0 0.7072184354074404 3.0235008079226085E-4 44-45 3.0235008079226085E-4 0.0 0.0 0.9071582245484942 3.0235008079226085E-4 46-47 4.3192868684608694E-4 0.0 0.0 1.1448485809198958 3.0235008079226085E-4 48-49 4.3192868684608694E-4 0.0 0.0 1.3960799016239223 3.0235008079226085E-4 50-51 4.3192868684608694E-4 0.0 0.0 1.6509178268631137 3.455429494768696E-4 52-53 4.3192868684608694E-4 4.31928686846087E-5 0.0 1.8980674214764446 3.8873581816147826E-4 54-55 4.3192868684608694E-4 4.31928686846087E-5 0.0 2.171413490946991 4.3192868684608694E-4 56-57 4.3192868684608694E-4 4.31928686846087E-5 0.0 2.4865918537385805 4.3192868684608694E-4 58-59 4.3192868684608694E-4 6.478930302691304E-5 0.0 2.8057655568834963 4.3192868684608694E-4 60-61 4.3192868684608694E-4 8.63857373692174E-5 0.0 3.1253927851496006 4.3192868684608694E-4 62-63 4.3192868684608694E-4 8.63857373692174E-5 0.0 3.4399448513452633 4.3192868684608694E-4 64-65 4.3192868684608694E-4 8.63857373692174E-5 0.0 3.7646472416818093 4.3192868684608694E-4 66-67 4.3192868684608694E-4 8.63857373692174E-5 0.0 4.115114178188724 4.5352512118839133E-4 68-69 4.7512155553069567E-4 8.63857373692174E-5 0.0 4.504238732168364 4.96717989873E-4 70-71 4.96717989873E-4 8.63857373692174E-5 0.0 4.939126130519346 5.61507292899913E-4 72-73 5.399108585576087E-4 8.63857373692174E-5 0.0 5.377339379759045 5.831037272422174E-4 74-75 5.61507292899913E-4 8.63857373692174E-5 0.0 5.821146105493399 6.047001615845217E-4 76-77 5.61507292899913E-4 8.63857373692174E-5 0.0 6.302487434114678 6.047001615845217E-4 78-79 5.61507292899913E-4 8.63857373692174E-5 0.0 6.836113730278676 6.047001615845217E-4 80-81 6.047001615845217E-4 8.63857373692174E-5 0.0 7.401465188491519 6.047001615845217E-4 82-83 6.047001615845217E-4 8.63857373692174E-5 0.0 7.9612447666440485 6.478930302691305E-4 84-85 6.694894646114347E-4 8.63857373692174E-5 0.0 8.502192254050087 6.478930302691305E-4 86-87 7.558752019806522E-4 8.63857373692174E-5 0.0 9.048862796556836 6.478930302691305E-4 88-89 7.774716363229565E-4 8.63857373692174E-5 0.0 9.642095251505596 6.478930302691305E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4615 0.0 43.803307 1 GTATCAA 7355 0.0 35.549862 1 ATCAACG 8975 0.0 28.911577 3 TCAACGC 9015 0.0 28.783293 4 TATCAAC 9140 0.0 28.706635 2 CAACGCA 9145 0.0 28.426003 5 AACGCAG 9515 0.0 27.32063 6 ACGCAGA 10900 0.0 23.892672 7 GTACATG 7410 0.0 23.502651 1 CGCAGAG 11095 0.0 23.47275 8 TACATGG 7465 0.0 23.135286 2 ACATGGG 7675 0.0 21.261747 3 GAGTACT 6620 0.0 21.211042 12-13 GCAGAGT 12590 0.0 19.969585 9 GTACTTT 7180 0.0 19.22635 14-15 AGAGTAC 10370 0.0 18.984228 10-11 AGTACTT 7270 0.0 18.36844 12-13 CAGAGTA 11895 0.0 18.364908 10-11 TGCACCG 350 1.3369572E-9 17.619528 5 TGGTATC 2690 0.0 17.285309 2 >>END_MODULE