FastQCFastQC Report
Fri 27 May 2016
SRR939797_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939797_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064796
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91680.8610099962809777No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71840.6746832257070838No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47210.4433713124391902No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26590.24971919503829842No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA20030.18811114992918831No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT17980.1688586358325914No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17390.1633176683608879No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC15890.14923046292435357No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14300.1342980251616272No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA13770.12932054590738506No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA13650.12819356947246233No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA13160.12359174902986111No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA12730.11955341680472127No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC12610.11842644036979853No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT12600.1183325256668883No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12590.11823861096397807No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA12330.11579682868831213No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA12010.1127915581951848No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT11630.10922279948459611No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT11010.10340008790416191No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10890.10227311146923918No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGAG352.808093E-454.206242
CGCGGAG802.2885615E-741.501658
ACGCGGA958.7993794E-734.948767
CCTTTAG4400.034.494881
CATTCCG4850.034.228179
CGCGGAC351.9842292E-434.1285192-93
CTTTAGG4450.034.1072962
TATTCGA300.003929992731.70231894-95
GGCATTC4950.031.6203087
TAGGCAT5200.031.0122225
GCGGAGT804.532134E-429.6440379
ATGGGAC3900.029.1879775
GGATGCC1153.908537E-628.8707168
GCGCAGA1153.908537E-628.8707161
TTAGGCA5800.028.6218284
GGTATCA74250.028.6179751
GGGATTC2002.3646862E-1128.4582757
GTACAGG856.46198E-427.9002691
TGGGACC1205.442831E-627.667776
GTACGCA700.00845147227.103121
Produced by FastQC (version 0.11.5)