##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939797_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1064796 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.103223528262692 31.0 30.0 33.0 16.0 34.0 2 30.02495971059245 31.0 30.0 33.0 25.0 34.0 3 30.238023997084888 31.0 30.0 34.0 26.0 34.0 4 34.0911676978501 35.0 35.0 37.0 30.0 37.0 5 34.09595077366932 35.0 35.0 37.0 30.0 37.0 6 34.16911032723639 36.0 35.0 37.0 30.0 37.0 7 34.137051604250956 36.0 35.0 37.0 30.0 37.0 8 34.035928947892366 36.0 35.0 37.0 30.0 37.0 9 35.30445925792358 38.0 35.0 39.0 29.0 39.0 10-11 35.200658154238 37.0 35.0 39.0 29.0 39.0 12-13 35.17309982381602 37.0 35.0 39.0 29.0 39.0 14-15 35.81774912753241 38.0 34.5 40.0 27.0 41.0 16-17 35.6834196409453 38.0 34.0 40.0 27.0 41.0 18-19 35.74520424569589 38.0 34.0 40.0 27.0 41.0 20-21 35.97982524352082 39.0 35.0 40.0 27.0 41.0 22-23 35.86286715953103 39.0 34.5 40.0 27.0 41.0 24-25 35.672190729491845 38.0 34.0 40.0 27.0 41.0 26-27 35.52369186210316 38.0 34.0 40.0 27.0 41.0 28-29 35.545050413412525 38.0 34.0 40.0 27.0 41.0 30-31 35.24261595648369 38.0 34.0 40.0 26.5 41.0 32-33 34.832561354475416 38.0 34.0 40.0 25.0 41.0 34-35 34.64265126841198 38.0 34.0 40.0 25.0 41.0 36-37 34.37206751340163 38.0 33.0 40.0 24.0 41.0 38-39 34.11907445181988 38.0 33.0 40.0 23.5 41.0 40-41 33.89852140691738 38.0 33.0 40.0 22.5 41.0 42-43 33.471758909687864 38.0 33.0 40.0 19.0 41.0 44-45 33.01845611741592 37.5 32.5 40.0 15.5 41.0 46-47 32.51194407191612 37.0 31.0 40.0 9.0 41.0 48-49 32.081539092934236 37.0 31.0 40.0 7.5 41.0 50-51 31.473620299099544 36.5 30.0 39.5 3.5 40.5 52-53 31.63224504975601 37.0 30.5 39.0 2.0 40.0 54-55 31.75604294155876 37.0 31.0 40.0 2.0 41.0 56-57 31.515244234576386 37.0 30.5 40.0 2.0 41.0 58-59 31.181970067505887 36.5 30.0 39.5 2.0 41.0 60-61 30.83026232254817 36.0 29.0 39.0 2.0 40.0 62-63 30.41589187036766 36.0 28.0 39.0 2.0 40.0 64-65 29.895629303641257 35.5 28.0 39.0 2.0 40.0 66-67 29.305499363258313 35.0 26.5 38.0 2.0 40.0 68-69 28.73721398277229 34.0 26.0 38.0 2.0 40.0 70-71 28.15558144470866 34.0 26.0 37.5 2.0 39.0 72-73 27.473030045191756 33.5 24.5 36.5 2.0 39.0 74-75 27.057409588315508 33.0 23.5 36.0 2.0 39.0 76-77 26.421992099895192 32.5 21.5 36.0 2.0 38.0 78-79 25.71795630336703 32.0 19.0 35.0 2.0 37.5 80-81 24.926820254771805 31.5 13.5 35.0 2.0 37.0 82-83 24.164386417680007 31.0 7.5 34.5 2.0 36.0 84-85 23.392104215267523 30.5 2.0 34.0 2.0 36.0 86-87 22.537914774285404 29.5 2.0 34.0 2.0 35.0 88-89 21.558190019496692 29.0 2.0 34.0 2.0 35.0 90-91 20.19766227521516 27.5 2.0 33.5 2.0 35.0 92-93 18.66493910570663 24.5 2.0 32.5 2.0 34.5 94-95 18.156109714912528 24.5 2.0 32.0 2.0 34.0 96-97 17.819079898872648 24.5 2.0 32.0 2.0 34.0 98-99 16.963464832700346 19.0 2.0 32.0 2.0 34.0 100-101 15.310350057663628 2.0 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.6231726516707283 1101 2 0.8447494382939738 1101 3 0.8234961156965959 1101 4 0.7707504993745715 1101 5 0.716128612854888 1101 6 0.8947865543483289 1101 7 0.7232042143080903 1101 8 0.7631791227235567 1101 9 0.8114303884524716 1101 10-11 0.8011312539356652 1101 12-13 0.796137552733363 1101 14-15 0.93963877819467 1101 16-17 0.6128010858881652 1101 18-19 0.8293119652136554 1101 20-21 0.6246857894716769 1101 22-23 0.8989509067211259 1101 24-25 0.9622164720605824 1101 26-27 0.8886844844417894 1101 28-29 0.9510722680103996 1101 30-31 0.9588689266640529 1101 32-33 1.3190280098614622 1101 34-35 0.8497090108864285 1101 36-37 1.050930324547089 1101 38-39 1.1105432592146016 1101 40-41 1.2639796618413683 1101 42-43 1.4567169157779034 1101 44-45 1.1499076136389377 1101 46-47 1.288238473200778 1101 48-49 0.9602248600729624 1101 50-51 1.0214778001467266 1101 52-53 1.0677837540274702 1101 54-55 1.0101387062802623 1101 56-57 1.250470327947692 1101 58-59 0.8251460533425963 1101 60-61 0.6894819225308169 1101 62-63 0.3648739754954704 1101 64-65 0.5312416882185147 1101 66-67 0.5743413718276464 1101 68-69 0.575698076746999 1101 70-71 0.28818674686969814 1101 72-73 0.2369463406522776 1101 74-75 0.38716668840670465 1101 76-77 0.10895213071887255 1101 78-79 -0.19561844314777588 1101 80-81 -0.5643383073817958 1101 82-83 -0.8642048234408612 1101 84-85 -0.9013402108561586 1101 86-87 -1.687753296946049 1101 88-89 -5.1336208197533075 1101 90-91 -8.095919356156326 1101 92-93 -10.33660619789191 1101 94-95 -8.499302304346099 1101 96-97 -5.059337363252082 1101 98-99 -3.667121299144174 1101 100-101 -2.9818158375205908 1102 1 2.015873645850462 1102 2 1.1291558545127458 1102 3 1.077875240707037 1102 4 0.9446832181915852 1102 5 0.8671074760925137 1102 6 0.8420490790635782 1102 7 0.7936113545793333 1102 8 0.8476426276944551 1102 9 1.1751344737792664 1102 10-11 1.030165061616188 1102 12-13 0.9921538717620209 1102 14-15 0.941327073608079 1102 16-17 1.048910417810525 1102 18-19 1.2028951894749795 1102 20-21 1.0713913507515755 1102 22-23 1.2393774014717494 1102 24-25 1.3706203540957276 1102 26-27 1.4129541661371832 1102 28-29 1.376909105572011 1102 30-31 1.3367233642702203 1102 32-33 1.1769535995465006 1102 34-35 1.261893282239015 1102 36-37 1.1157504253574757 1102 38-39 0.8507372217309594 1102 40-41 1.0221983535342574 1102 42-43 0.7817712197128515 1102 44-45 0.7959886796669551 1102 46-47 0.8172040528604683 1102 48-49 0.6772247765284405 1102 50-51 1.0619829659825868 1102 52-53 0.8228590833321014 1102 54-55 0.890085237790462 1102 56-57 0.8404965052291509 1102 58-59 0.7526120089526067 1102 60-61 0.9046577837355301 1102 62-63 0.5731260849944846 1102 64-65 0.7027307299629264 1102 66-67 0.9476495953927682 1102 68-69 0.5035295396114634 1102 70-71 0.7469401234605257 1102 72-73 0.5895947017873695 1102 74-75 0.36093719162316873 1102 76-77 0.4631042931294118 1102 78-79 0.4171840576180479 1102 80-81 0.21031428667540553 1102 82-83 0.2754920492094506 1102 84-85 0.2697659185264456 1102 86-87 0.3422287409831597 1102 88-89 -0.016707232630302826 1102 90-91 -5.493246627870938 1102 92-93 -10.366351526361097 1102 94-95 -7.901614398807098 1102 96-97 -4.176358862040686 1102 98-99 -2.362218628504502 1102 100-101 -1.3329151441011131 1103 1 1.8642325873705161 1103 2 1.0077810076621674 1103 3 0.8265390004651074 1103 4 0.5457581327795467 1103 5 0.7097300233883885 1103 6 0.5062567182120858 1103 7 0.6402814883144003 1103 8 0.6159187667374653 1103 9 0.7664531689277183 1103 10-11 0.7222190590473545 1103 12-13 0.610431028716576 1103 14-15 0.7368790777833638 1103 16-17 0.582645394361542 1103 18-19 0.5010396292719932 1103 20-21 0.5700972150324972 1103 22-23 0.5491815552911348 1103 24-25 0.47471430177877494 1103 26-27 0.545102681281854 1103 28-29 0.5474597216861028 1103 30-31 0.7481308213050966 1103 32-33 0.5825668264741495 1103 34-35 0.4999920455690301 1103 36-37 0.43654524770415293 1103 38-39 0.6466515098776568 1103 40-41 0.5695515984511843 1103 42-43 0.37120631582221364 1103 44-45 0.2555419645600381 1103 46-47 0.23732066850817546 1103 48-49 0.03998346486613258 1103 50-51 0.08055661280799953 1103 52-53 -0.29120854671072394 1103 54-55 -0.3230339763077943 1103 56-57 -0.24438760091496192 1103 58-59 0.07153665018505606 1103 60-61 -0.21342041778134302 1103 62-63 -0.3222020711790563 1103 64-65 -0.4392170345182542 1103 66-67 -0.3694423423776705 1103 68-69 -0.3666779477756883 1103 70-71 -0.44159745228134994 1103 72-73 -0.3366284577360261 1103 74-75 -0.7466551329536593 1103 76-77 -0.7881084096663287 1103 78-79 -0.9669725193860366 1103 80-81 -1.0501873216248327 1103 82-83 -1.1189921657586588 1103 84-85 -0.8913292247399767 1103 86-87 -1.0165711968316984 1103 88-89 -1.2497802890670187 1103 90-91 -6.516906130908016 1103 92-93 -9.743772767768998 1103 94-95 -8.360445429192682 1103 96-97 -5.254087975421227 1103 98-99 -3.9335608096483377 1103 100-101 -2.9630361645296723 1104 1 2.0014966132161653 1104 2 1.3050614281361632 1104 3 0.9756304648108092 1104 4 0.8412777380226188 1104 5 0.8246025230066536 1104 6 0.7702536321643976 1104 7 0.7377274623883849 1104 8 0.8570572290177694 1104 9 0.9868218873339032 1104 10-11 0.9753633719597232 1104 12-13 0.8215275400361008 1104 14-15 0.7417821967810383 1104 16-17 0.6556830618238294 1104 18-19 0.6897237192495709 1104 20-21 0.6217941394323674 1104 22-23 0.6485321994232223 1104 24-25 0.7580142832946279 1104 26-27 0.6591739336225118 1104 28-29 0.6503315944509325 1104 30-31 0.7747873618777845 1104 32-33 0.8868812658019678 1104 34-35 0.8894922505018812 1104 36-37 0.7553395995939525 1104 38-39 0.6096378235776214 1104 40-41 0.5026775879554037 1104 42-43 0.4712099322843528 1104 44-45 0.4936345620638747 1104 46-47 0.44022468760309863 1104 48-49 0.27462113554305745 1104 50-51 0.4813831753205591 1104 52-53 0.20373363007801615 1104 54-55 0.17465590804578213 1104 56-57 0.3649376476163546 1104 58-59 0.21843010921054073 1104 60-61 0.17293467058088297 1104 62-63 0.16353018203758296 1104 64-65 0.2820156165092236 1104 66-67 0.24261333506605354 1104 68-69 0.03707875272499095 1104 70-71 -0.13044950683367063 1104 72-73 0.02163556007304024 1104 74-75 -0.22189522585655297 1104 76-77 0.07239460003382447 1104 78-79 -0.1403007093065547 1104 80-81 -0.19134266072843076 1104 82-83 -0.387000941706777 1104 84-85 -0.46951392147953186 1104 86-87 -0.35404055736082896 1104 88-89 -0.4767068588125021 1104 90-91 -3.057065737436936 1104 92-93 -7.567123460120582 1104 94-95 -5.519991281914269 1104 96-97 -2.927969082219372 1104 98-99 -2.1159558719720373 1104 100-101 -1.6051787977963006 1105 1 2.1093110574947005 1105 2 1.3872673910051105 1105 3 1.0472993712863463 1105 4 0.9072127337918658 1105 5 0.8011243486997444 1105 6 0.8843146545127141 1105 7 0.8439292442807727 1105 8 0.8308216011246827 1105 9 0.9461220714959637 1105 10-11 0.9606801804809564 1105 12-13 0.7422407947272589 1105 14-15 0.9404607095479562 1105 16-17 0.8776406547759024 1105 18-19 0.7187591082297118 1105 20-21 0.7352927208867541 1105 22-23 0.709676492887958 1105 24-25 0.7337720852955272 1105 26-27 0.7174338509984537 1105 28-29 0.8148356012201319 1105 30-31 1.0854911595981633 1105 32-33 1.163900826799427 1105 34-35 1.002259161058852 1105 36-37 1.1072434371331497 1105 38-39 1.1654040870786915 1105 40-41 1.324025455159628 1105 42-43 1.3917688890311481 1105 44-45 1.158429743986126 1105 46-47 1.101737305193062 1105 48-49 1.0166847461149544 1105 50-51 1.0220108195393607 1105 52-53 0.9812260396146293 1105 54-55 1.054686767634653 1105 56-57 1.1443438493982505 1105 58-59 0.8898700574460712 1105 60-61 1.0939540324842234 1105 62-63 0.931204762695593 1105 64-65 0.8389839107703345 1105 66-67 0.7470705547166396 1105 68-69 0.750888418744605 1105 70-71 0.45707973511546385 1105 72-73 0.4983838553296245 1105 74-75 0.6304939036043997 1105 76-77 0.6113175008351774 1105 78-79 0.22076394650948217 1105 80-81 0.09782164169553909 1105 82-83 -0.18701836244242642 1105 84-85 -0.09712827616571218 1105 86-87 0.01212588532495218 1105 88-89 -0.07797359516210989 1105 90-91 -2.5675256448581685 1105 92-93 -7.329213450663611 1105 94-95 -5.026053697921437 1105 96-97 -2.7321376153934303 1105 98-99 -1.5881772646354193 1105 100-101 -1.1445397056654958 1106 1 1.6591932242706449 1106 2 0.9829723657527119 1106 3 0.8188206651307262 1106 4 0.6348197621074192 1106 5 0.803004936459665 1106 6 0.6928623494373269 1106 7 0.5706662889794671 1106 8 -0.5720409584060846 1106 9 -0.7680692503539248 1106 10-11 -0.7088116033836016 1106 12-13 -0.3883744963148743 1106 14-15 0.44935241649477575 1106 16-17 0.6949178347752607 1106 18-19 0.9386023586985814 1106 20-21 0.7157058063891171 1106 22-23 0.7836043460869107 1106 24-25 0.7600067081021464 1106 26-27 0.7907369593897684 1106 28-29 0.5430593267830801 1106 30-31 0.7258859305476477 1106 32-33 0.763199672525154 1106 34-35 0.8700462175323977 1106 36-37 0.7456360598076159 1106 38-39 0.7593974728312887 1106 40-41 0.34860778678348936 1106 42-43 0.5118884673051127 1106 44-45 0.7400794430073745 1106 46-47 0.5882529826875071 1106 48-49 0.38360859586446594 1106 50-51 0.34949484725007807 1106 52-53 0.4180929295799096 1106 54-55 0.6836412963779956 1106 56-57 0.42613467721264797 1106 58-59 0.6998095567353424 1106 60-61 0.31112930495766733 1106 62-63 0.3057593937700176 1106 64-65 0.34474384611877795 1106 66-67 0.29025333937579134 1106 68-69 0.24173368364487047 1106 70-71 0.037972963474725674 1106 72-73 -0.019543567536626227 1106 74-75 0.015780168028594943 1106 76-77 -0.3261711690471252 1106 78-79 -0.4995397091159326 1106 80-81 -0.622546817967546 1106 82-83 -0.9146893476840496 1106 84-85 -1.0030370490229004 1106 86-87 -0.5915978478620012 1106 88-89 -1.0191378822922843 1106 90-91 -3.953982617518488 1106 92-93 -8.916287205282487 1106 94-95 -6.690315838301384 1106 96-97 -3.8372002084698167 1106 98-99 -2.642065422184622 1106 100-101 -2.043738931066647 1107 1 2.087476342945898 1107 2 1.253396370221104 1107 3 0.9489814944118393 1107 4 0.979445369940251 1107 5 1.016751815155942 1107 6 0.8122661804269455 1107 7 0.612453974614894 1107 8 0.8236836768942126 1107 9 0.9264052206672346 1107 10-11 0.7775754054217643 1107 12-13 0.8723480033565636 1107 14-15 0.9307461607450094 1107 16-17 0.924899599790372 1107 18-19 0.8950687964696513 1107 20-21 1.0130666619548876 1107 22-23 0.6768422058582289 1107 24-25 0.8231285046588539 1107 26-27 0.8345198152183926 1107 28-29 0.9163467167160562 1107 30-31 0.7044286102690336 1107 32-33 0.8486211306668352 1107 34-35 0.9568788335134641 1107 36-37 0.8766314271357771 1107 38-39 0.7933572572970533 1107 40-41 0.7851985017263203 1107 42-43 1.2153219013963223 1107 44-45 0.755402580081892 1107 46-47 0.6532073130857228 1107 48-49 0.7316594238313456 1107 50-51 0.7333096553720395 1107 52-53 0.723392574736966 1107 54-55 0.7608487973636748 1107 56-57 0.7933843979381088 1107 58-59 0.7463379277433582 1107 60-61 0.5666002111214254 1107 62-63 0.8310173120247129 1107 64-65 0.7562836345272395 1107 66-67 0.7644360506387677 1107 68-69 0.8354056510518895 1107 70-71 0.9335546759205116 1107 72-73 0.7702037711412544 1107 74-75 0.7290740469877228 1107 76-77 0.7347940530582804 1107 78-79 0.8774759959701548 1107 80-81 0.7148952736345038 1107 82-83 0.48462067991484403 1107 84-85 0.5670891994985894 1107 86-87 0.9238029111076393 1107 88-89 0.37957129434064996 1107 90-91 -2.1699156409118388 1107 92-93 -3.5158847213818447 1107 94-95 -1.6874824337804206 1107 96-97 -0.46151557514086505 1107 98-99 0.03003833652217125 1107 100-101 0.5584915689990648 1108 1 1.6126097759091742 1108 2 0.7494886905822149 1108 3 -0.5003437160310042 1108 4 -0.16174424211294536 1108 5 0.4097980515839055 1108 6 0.5510646737781713 1108 7 0.567130990596354 1108 8 0.5118752408810394 1108 9 0.6500442044417909 1108 10-11 0.5608566363599081 1108 12-13 0.5883553841056184 1108 14-15 0.4554472709072641 1108 16-17 0.32796093320504127 1108 18-19 0.364717372168073 1108 20-21 0.28803997536223847 1108 22-23 0.356614149862132 1108 24-25 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72-73 1.3945112297377023 2311 74-75 1.487122048298339 2311 76-77 1.665803897571564 2311 78-79 1.8290889472390681 2311 80-81 1.871317664317214 2311 82-83 1.9730693871708915 2311 84-85 2.1078921751926174 2311 86-87 2.678522990340337 2311 88-89 3.1846197722389036 2311 90-91 3.701117884049438 2311 92-93 4.494509357016824 2311 94-95 4.392642744939938 2311 96-97 3.5585684978961893 2311 98-99 3.3687566136578795 2311 100-101 3.052674593543328 2312 1 1.2049062779580808 2312 2 0.4067959002199437 2312 3 -0.0962191264538923 2312 4 -0.6900400948386789 2312 5 -0.16848666691438297 2312 6 -0.09274063749112571 2312 7 0.14503346863833855 2312 8 0.4318107802944695 2312 9 0.39919665039916197 2312 10-11 0.24829389793655565 2312 12-13 0.10866719797186164 2312 14-15 0.08056338251592621 2312 16-17 0.0345397007535837 2312 18-19 -0.3867697929466303 2312 20-21 0.23107539469250327 2312 22-23 0.11352586896604322 2312 24-25 0.3381344522044003 2312 26-27 0.403599106154914 2312 28-29 0.277047221532122 2312 30-31 0.40419288437835377 2312 32-33 0.7508119308293075 2312 34-35 0.43788621717367704 2312 36-37 0.4843784678692131 2312 38-39 0.33093414020293466 2312 40-41 0.10513365897362092 2312 42-43 0.5271444614520249 2312 44-45 0.2820831243548412 2312 46-47 0.4239700495369263 2312 48-49 0.26705239702387473 2312 50-51 0.1823642132227974 2312 52-53 0.19265972107559648 2312 54-55 0.2640028097809868 2312 56-57 0.47940585262071167 2312 58-59 0.33837873370831417 2312 60-61 0.2625816286681335 2312 62-63 0.35844129949134995 2312 64-65 0.5374828627969386 2312 66-67 0.6298931412735591 2312 68-69 0.5462388876771662 2312 70-71 0.6938678649654264 2312 72-73 0.4141996513779951 2312 74-75 0.6283773863169557 2312 76-77 0.9181601462461622 2312 78-79 0.5890330924074298 2312 80-81 0.9248933152963339 2312 82-83 0.9426629219944545 2312 84-85 1.3668755955761078 2312 86-87 1.4122751897428962 2312 88-89 2.1396469442188 2312 90-91 2.8167832537150623 2312 92-93 4.006180672606785 2312 94-95 3.63261628096949 2312 96-97 2.893012175236297 2312 98-99 2.477962498063313 2312 100-101 2.2390623157786838 2313 1 -7.712647829239533 2313 2 -2.7883787007344303 2313 3 -1.4406767364301665 2313 4 -0.5043117256132064 2313 5 -0.3558356966699776 2313 6 -0.20530122733085676 2313 7 8.054410292785974E-4 2313 8 0.10382204018655017 2313 9 -0.017261997875813506 2313 10-11 0.02236608659489292 2313 12-13 -0.17242493893293442 2313 14-15 -0.21503198495036457 2313 16-17 -0.3202931627451093 2313 18-19 -0.27437597647264766 2313 20-21 -0.23035221221729074 2313 22-23 -0.7958133050216176 2313 24-25 0.04178389727947973 2313 26-27 -0.006474744857293047 2313 28-29 -0.38122305622624 2313 30-31 0.05760387043932269 2313 32-33 -0.19502539251197248 2313 34-35 -0.06710118067764625 2313 36-37 0.09755739686141851 2313 38-39 -0.23373593097162626 2313 40-41 0.0106656256619857 2313 42-43 0.008935903288460167 2313 44-45 -0.16905934044790882 2313 46-47 -1.0039828477468191 2313 48-49 -0.3734316357713041 2313 50-51 -0.5806656284501024 2313 52-53 -0.8187195350075989 2313 54-55 -0.6260451241121991 2313 56-57 -0.3652514819105228 2313 58-59 -0.15746706279590583 2313 60-61 -0.0323029345739414 2313 62-63 -0.04670265013233177 2313 64-65 -0.15244980918136974 2313 66-67 -0.1071166929752323 2313 68-69 0.00995626599985755 2313 70-71 -0.07017640260361091 2313 72-73 -0.114138707572085 2313 74-75 0.14320306780134473 2313 76-77 0.31071943148779724 2313 78-79 0.38852140751225406 2313 80-81 0.5970410872579635 2313 82-83 1.121872335543987 2313 84-85 1.166376687672667 2313 86-87 1.645222613094397 2313 88-89 1.8644429830823768 2313 90-91 2.7755165965526842 2313 92-93 3.659860311730739 2313 94-95 3.5050228878846674 2313 96-97 2.6140786774608387 2313 98-99 2.414451770770043 2313 100-101 2.374991687258289 2314 1 0.7491492910195987 2314 2 -0.07472489084613443 2314 3 -1.1021865481820825 2314 4 -0.1722221940702795 2314 5 -0.13960137938820338 2314 6 -3.285479990905909 2314 7 -2.1225514094482776 2314 8 -2.466040240310857 2314 9 -2.744166387372964 2314 10-11 -2.2425505218335573 2314 12-13 -1.0359943034153858 2314 14-15 -1.4930813099417648 2314 16-17 -1.9862020393577495 2314 18-19 -3.1780430424870403 2314 20-21 -2.6298039410601817 2314 22-23 -3.8951524399900563 2314 24-25 -2.8442515503782317 2314 26-27 -1.5974809624796151 2314 28-29 -1.7077335058252245 2314 30-31 -2.118206888654157 2314 32-33 -2.050682680053434 2314 34-35 -1.6365772863716685 2314 36-37 -2.246826636994392 2314 38-39 -3.5168006752226866 2314 40-41 -2.7690504214610456 2314 42-43 -1.857345004562049 2314 44-45 -2.321178240688628 2314 46-47 -1.8664415263495506 2314 48-49 -2.739686891076527 2314 50-51 -3.181243018712671 2314 52-53 -2.1947158244015874 2314 54-55 -2.478608160042402 2314 56-57 -1.6323596496809465 2314 58-59 -1.187468754477269 2314 60-61 -1.5932249563827625 2314 62-63 -1.1733048942447297 2314 64-65 -1.7793633503973858 2314 66-67 -1.2181838943241416 2314 68-69 -1.3045799914463139 2314 70-71 -1.2062021438928383 2314 72-73 -1.818289289399548 2314 74-75 -1.8811758632778925 2314 76-77 -1.1520157399544075 2314 78-79 -2.066311393357438 2314 80-81 -0.8983054023645423 2314 82-83 -0.5866523519109528 2314 84-85 -1.6666980402716902 2314 86-87 -0.43280826979688314 2314 88-89 0.5418566796002864 2314 90-91 1.7327232978294873 2314 92-93 2.940375747629883 2314 94-95 2.4909327074016794 2314 96-97 1.5459794894678929 2314 98-99 1.2671375229304047 2314 100-101 1.2642607422462522 2315 1 -2.699969497608695 2315 2 -1.6897439491648818 2315 3 -2.6244788299258666 2315 4 -3.365923223771066 2315 5 -3.576687154886148 2315 6 -2.852590529154636 2315 7 -5.4849308978782965 2315 8 -2.881473849721285 2315 9 -2.2775917464038216 2315 10-11 -3.695866349963236 2315 12-13 -2.7309479857496513 2315 14-15 -1.9510129380418206 2315 16-17 -2.523260526478502 2315 18-19 -1.7015756390341892 2315 20-21 -1.2727543952374774 2315 22-23 -0.6798805287295338 2315 24-25 -2.020856702075484 2315 26-27 -3.8650855945364953 2315 28-29 -3.225125491751392 2315 30-31 -2.974803563536362 2315 32-33 -3.658652482851366 2315 34-35 -3.7162433811436273 2315 36-37 -2.849490447090517 2315 38-39 -1.7504407124974435 2315 40-41 -1.4866091858201074 2315 42-43 -2.778284189220173 2315 44-45 -6.121276362725734 2315 46-47 -2.7420297505438924 2315 48-49 -1.9018219453194334 2315 50-51 -2.7762479393296218 2315 52-53 -2.3515702873576174 2315 54-55 -1.4554493271721043 2315 56-57 -1.0130644445971768 2315 58-59 -2.525651580798357 2315 60-61 -2.8633878738005905 2315 62-63 -4.330424257670757 2315 64-65 -3.6495330899095 2315 66-67 -3.1598629713963895 2315 68-69 -1.6400833313694996 2315 70-71 -3.164407381519446 2315 72-73 -2.0207871919684273 2315 74-75 -1.3787612773499838 2315 76-77 -3.041552969719639 2315 78-79 -1.3117013994247486 2315 80-81 -0.9946870796707721 2315 82-83 -0.6515145020608912 2315 84-85 -1.898820524442982 2315 86-87 -1.3911180161020624 2315 88-89 -0.8835190436142852 2315 90-91 -0.05700016059492796 2315 92-93 0.7239549666597505 2315 94-95 0.4456964161824395 2315 96-97 -0.013599166376319971 2315 98-99 -0.39424056914754857 2315 100-101 -0.5701982366458953 2316 1 -7.339920610459121 2316 2 -6.3466580332607165 2316 3 -3.459591854282479 2316 4 -3.8434456950879117 2316 5 -2.108977831541445 2316 6 -2.774520173899731 2316 7 -2.88376338329601 2316 8 -2.2689526518308227 2316 9 -7.118196033813049 2316 10-11 -6.403232966314029 2316 12-13 -5.779591320760179 2316 14-15 -4.863787889434207 2316 16-17 -4.194573646497389 2316 18-19 -3.7049968077290245 2316 20-21 -2.59555789001643 2316 22-23 -3.0588832805729 2316 24-25 -2.7397843217620306 2316 26-27 -3.9452772917964545 2316 28-29 -4.112695141962902 2316 30-31 -6.7923999867611755 2316 32-33 -4.734723403383349 2316 34-35 -5.529966724602254 2316 36-37 -4.128434497083369 2316 38-39 -6.598373413465872 2316 40-41 -4.015911969343829 2316 42-43 -4.9244502440209885 2316 44-45 -3.5471287819252737 2316 46-47 -4.459843122142132 2316 48-49 -5.348112081544997 2316 50-51 -4.912797295472817 2316 52-53 -6.354590016148855 2316 54-55 -5.167797836020863 2316 56-57 -4.661481513480229 2316 58-59 -3.2798189462965794 2316 60-61 -3.641184287086464 2316 62-63 -4.5327499690812765 2316 64-65 -3.2265792686985684 2316 66-67 -4.037256857707366 2316 68-69 -3.074860605988558 2316 70-71 -2.560905769547997 2316 72-73 -2.1513592088894704 2316 74-75 -2.2129782443589257 2316 76-77 -2.508311105671879 2316 78-79 -4.429865273871119 2316 80-81 -5.586511216380856 2316 82-83 -3.228103334422073 2316 84-85 -2.154193762610987 2316 86-87 -2.2879914437310696 2316 88-89 -1.3112777199962657 2316 90-91 -1.0620255359451214 2316 92-93 -1.1481875887653565 2316 94-95 -1.8240719755110923 2316 96-97 -3.2151942105735323 2316 98-99 -4.7779557751009705 2316 100-101 -4.139509903095147 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9423.0 3 2039.0 4 1620.0 5 1691.0 6 2074.0 7 2458.0 8 3106.0 9 4024.0 10 5064.0 11 6771.0 12 9106.0 13 11113.0 14 12853.0 15 15555.0 16 18094.0 17 19549.0 18 15700.0 19 13224.0 20 13825.0 21 15109.0 22 16734.0 23 18869.0 24 20962.0 25 23987.0 26 27318.0 27 31045.0 28 35619.0 29 40622.0 30 46472.0 31 53134.0 32 61252.0 33 69113.0 34 79430.0 35 95878.0 36 113867.0 37 109554.0 38 37913.0 39 629.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.88722412606221 25.08764769388761 15.024620350064138 20.000507829986045 2 14.290496353069168 23.721834664319694 41.76504494135855 20.222624041252583 3 15.70747486905579 31.50954928862266 33.71806324816866 19.064912594152883 4 10.223752442825194 20.898828008178175 43.688905858830566 25.188513690166065 5 9.473756468376772 41.44143127558318 37.67733672608687 11.40747552995318 6 22.80756038631441 44.43904186953753 19.86938517548075 12.884012568667302 7 22.44248267142309 37.73095361736395 25.48495555906768 14.341608152145282 8 23.149294333219203 36.7868551454791 22.584347027771404 17.47950349353029 9 25.91376940639914 17.500496243133735 24.808770003772388 31.776964346694736 10-11 20.733510086151714 29.678764216537722 31.3632308044699 18.224494892840664 12-13 22.730596867168096 29.484757461133537 30.27678349297542 17.507862178722945 14-15 21.14831545753194 30.086137768695515 29.216670703517234 19.548876070255307 16-17 17.117167112111474 33.211872612974695 30.77152796426158 18.89943231065225 18-19 17.08008507940211 31.440573768564086 34.470328041113554 17.00901311092025 20-21 17.580457994010132 31.003568802821174 34.41770939022721 16.998263812941484 22-23 18.155210471904844 30.575963236529326 33.5952752578285 17.673551033737336 24-25 17.688754507826747 31.952031357812483 33.741183203915206 16.618030930445563 26-27 17.200564118061852 31.156978654457916 34.81211912380942 16.830338103670815 28-29 16.884654905170763 30.352557751154034 35.65087928800289 17.111908055672316 30-31 17.597349664330977 30.8261072559481 34.67100482324192 16.905538256478998 32-33 17.115683936899583 31.43254245233511 34.59372883501822 16.858044775747093 34-35 17.580198992706485 31.104681533548373 34.32492416120837 16.990195312536766 36-37 18.363389658841843 30.19753540797832 33.56704112940308 17.87203380377676 38-39 16.951105133445683 31.575516942304915 33.91152814634906 17.561849777900342 40-41 16.70255902473184 30.718603285280246 35.39775572363889 17.181081966349034 42-43 16.838288096239765 30.740141532385955 34.4656018326364 17.955968538737878 44-45 16.98429361745578 30.675277046208173 34.10664567400421 18.23378366233183 46-47 17.64132278389271 30.74901216649442 33.24240935368675 18.367255695926115 48-49 17.043530171129255 30.9590045888383 33.29876129142819 18.69870394860426 50-51 16.891623725597743 30.639984746014072 33.11649760950267 19.35189391888552 52-53 16.914030131826742 31.366431261770245 33.25414312617703 18.465395480225986 54-55 17.402046867599548 30.80447226163898 32.668573736856324 19.124907133905143 56-57 17.41856529955685 31.41801459599775 32.200437268039664 18.962982836405743 58-59 16.94344919257512 30.16490219963738 32.859425040432434 20.03222356735507 60-61 17.55095261017871 30.83267573609717 32.139306151392766 19.477065502331353 62-63 17.44663612440428 30.110084827942167 32.748601296438586 19.69467775121497 64-65 17.484325852873575 29.84785784695445 32.69298903927182 19.97482726090015 66-67 17.193473812101765 30.26071907022574 31.714114836475922 20.831692281196577 68-69 17.352686247328432 29.647819126860213 32.17756044491751 20.821934180893845 70-71 17.385710541213477 29.829164041202418 32.363115470388294 20.422009947195814 72-73 17.749318380381407 29.020896725213145 32.018045973548645 21.211738920856806 74-75 17.15522829434867 30.01458835558305 31.532172761153454 21.298010588914824 76-77 17.31020936245864 29.415029953313887 32.294823095596364 20.979937588631113 78-79 17.441248420453967 29.085256879852807 31.780277849444545 21.693216850248678 80-81 17.433188871749376 29.181993343843054 31.462772419326583 21.92204536508098 82-83 17.70393571468258 29.271036292802183 31.444658632571514 21.580369359943724 84-85 17.307395377300757 28.648495975144755 31.39147013133738 22.65263851621711 86-87 17.24150430533894 28.599633842586208 31.99099152960871 22.167870322466143 88-89 18.3891255049636 27.93716866361009 31.77618666740735 21.89751916401896 90-91 18.16338890188591 27.925933332830223 31.933066810556266 21.977610954727602 92-93 18.964953650692877 27.787146522762747 31.309684416580698 21.938215409963686 94-95 18.302319773441585 26.767395944755183 32.034360731131514 22.89592355067171 96-97 19.017664982269086 26.594440075162122 31.038659514648607 23.349235427920185 98-99 18.6110917446924 26.715608223839787 31.083039225477243 23.590260805990567 100-101 18.885722712696836 26.58749341557853 31.451861400637192 23.07492247108744 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2486.0 1 1572.5 2 667.0 3 668.5 4 564.0 5 558.0 6 856.0 7 1482.0 8 2219.5 9 2481.5 10 2211.5 11 1979.0 12 1974.0 13 1992.5 14 1961.5 15 1966.5 16 2062.5 17 2220.0 18 2441.5 19 2718.5 20 3081.5 21 3590.5 22 4152.5 23 4884.0 24 5632.0 25 7231.0 26 11018.0 27 14825.0 28 15827.0 29 17028.5 30 20895.0 31 25280.0 32 30816.5 33 38661.0 34 47969.5 35 55082.0 36 62328.0 37 69519.5 38 69243.0 39 64517.0 40 58635.0 41 52567.0 42 47728.5 43 44970.5 44 43116.5 45 40467.5 46 35646.5 47 29728.5 48 27790.0 49 24458.0 50 17537.5 51 11653.5 52 7244.0 53 4590.0 54 3017.0 55 2041.5 56 1490.0 57 1175.0 58 1009.0 59 885.0 60 742.0 61 616.5 62 512.5 63 367.5 64 263.5 65 227.0 66 200.0 67 159.0 68 121.0 69 98.5 70 78.0 71 58.5 72 45.5 73 34.5 74 27.0 75 20.0 76 13.0 77 11.0 78 9.0 79 4.0 80 2.5 81 1.5 82 1.0 83 1.0 84 1.0 85 1.0 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 1.0 92 1.0 93 0.5 94 0.5 95 0.5 96 0.5 97 1.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13598848981401132 2 0.12199519908038722 3 0.12828748417537256 4 0.13880593090131818 5 0.1270665930375396 6 0.14246860431481712 7 0.17017344167333462 8 0.15852801851246626 9 0.16989169756460393 10-11 0.16242547868324073 12-13 0.15369141131258945 14-15 0.1571662553202679 16-17 0.15275226428348715 18-19 0.16801340350639934 20-21 0.17228652248881476 22-23 0.20844368310925285 24-25 0.23262671910863675 26-27 0.24483563048696652 28-29 0.23896596155507718 30-31 0.22915187510095827 32-33 0.23079538240188732 34-35 0.22022997832448657 36-37 0.22816577072040092 38-39 0.2181168975090064 40-41 0.22163869886814 42-43 0.2144072667440524 44-45 0.20684713315977898 46-47 0.22257784589724228 48-49 0.2594393667895071 50-51 0.26094200203607076 52-53 0.26258550933699976 54-55 0.2613646181991668 56-57 0.23652417927941127 58-59 0.23760419836287888 60-61 0.20806802429761193 62-63 0.19867655400658907 64-65 0.20083659217352431 66-67 0.20806802429761193 68-69 0.20820889635197729 70-71 0.21464205350132792 72-73 0.2460565216247995 74-75 0.24863917595483082 76-77 0.263900315177743 78-79 0.26127070349625653 80-81 0.2744187619036886 82-83 0.27282221195421474 84-85 0.24849830390046546 86-87 0.25211401996250926 88-89 0.2549314610498161 90-91 0.44365305654792087 92-93 0.5927896047693643 94-95 0.34757831547075685 96-97 0.29029034669551723 98-99 0.31874650167731655 100-101 0.33612072171570895 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1064796.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.35485892790861 #Duplication Level Percentage of deduplicated Percentage of total 1 81.42685604404969 21.45993303984052 2 7.571188022579635 3.9907518450351525 3 2.818701157003985 2.2285941405831835 4 1.4671451688726551 1.5466561580960592 5 0.9406254130025212 1.2395025031843607 6 0.6586049830336814 1.0414464850242189 7 0.4922020944214158 0.9080341733748297 8 0.40761461574462876 0.8594100555922694 9 0.3250224382605007 0.7709328457884344 >10 2.7409436929172415 15.554010479866811 >50 0.576477388357722 10.765661554548643 >100 0.5233269898530989 27.08121785659423 >500 0.04199996438451717 7.528802491617236 >1k 0.00854866531720261 3.4232693721561245 >5k 7.43362201495879E-4 1.6017769986979409 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9168 0.8610099962809777 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7184 0.6746832257070838 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4721 0.4433713124391902 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2659 0.24971919503829842 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 2003 0.18811114992918831 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1798 0.1688586358325914 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1739 0.1633176683608879 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1589 0.14923046292435357 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1430 0.1342980251616272 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1377 0.12932054590738506 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1365 0.12819356947246233 No Hit CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA 1316 0.12359174902986111 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1273 0.11955341680472127 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1261 0.11842644036979853 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1260 0.1183325256668883 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1259 0.11823861096397807 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 1233 0.11579682868831213 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 1201 0.1127915581951848 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 1163 0.10922279948459611 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 1101 0.10340008790416191 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1089 0.10227311146923918 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 9.391470291022881E-5 0.0 12-13 0.0 0.0 0.0 2.8174410873068646E-4 0.0 14-15 0.0 0.0 0.0 2.8174410873068646E-4 0.0 16-17 0.0 0.0 0.0 3.2870146018580085E-4 0.0 18-19 0.0 0.0 0.0 3.7565881164091524E-4 0.0 20-21 0.0 0.0 0.0 7.043602718267161E-4 0.0 22-23 0.0 0.0 0.0 0.002535696978576178 0.0 24-25 0.0 0.0 0.0 0.007607090935728533 0.0 26-27 0.0 0.0 0.0 0.018219452364584388 0.0 28-29 0.0 0.0 0.0 0.045595588262916084 0.0 30-31 0.0 0.0 0.0 0.12424915195023273 0.0 32-33 0.0 0.0 0.0 0.2862050571189223 0.0 34-35 0.0 0.0 0.0 0.53968084027363 0.0 36-37 0.0 0.0 0.0 0.9201293017629668 0.0 38-39 0.0 0.0 0.0 1.4265173798549204 0.0 40-41 0.0 0.0 0.0 1.9685460877013061 0.0 42-43 0.0 0.0 0.0 2.4799586023989573 0.0 44-45 0.0 0.0 0.0 3.0776787290711085 0.0 46-47 0.0 0.0 0.0 3.7930270211383212 0.0 48-49 0.0 0.0 0.0 4.500110819349434 0.0 50-51 0.0 0.0 0.0 5.217149576069032 0.0 52-53 0.0 0.0 0.0 5.8738011788173505 0.0 54-55 0.0 0.0 0.0 6.553461883778677 0.0 56-57 0.0 0.0 0.0 7.346477635152649 0.0 58-59 0.0 0.0 0.0 8.168559987077337 0.0 60-61 0.0 0.0 0.0 9.009096578123884 0.0 62-63 0.0 0.0 0.0 9.775628383277173 0.0 64-65 0.0 0.0 0.0 10.554228227754425 0.0 66-67 0.0 0.0 0.0 11.417163475445061 0.0 68-69 0.0 0.0 0.0 12.312170594179543 0.0 70-71 0.0 0.0 0.0 13.30409768631738 0.0 72-73 0.0 0.0 0.0 14.209623251777806 0.0 74-75 0.0 0.0 0.0 15.122145462605044 0.0 76-77 0.0 0.0 0.0 16.063264700468448 0.0 78-79 0.0 4.6957351455114406E-5 0.0 17.080267018283315 0.0 80-81 0.0 9.391470291022881E-5 0.0 18.093231003873044 0.0 82-83 0.0 9.391470291022881E-5 0.0 18.99922614284802 0.0 84-85 0.0 9.391470291022881E-5 0.0 19.85878046123389 0.0 86-87 0.0 9.391470291022881E-5 0.0 20.75975116360317 0.0 88-89 0.0 9.391470291022881E-5 0.0 21.676922152224464 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCTGAG 35 2.808093E-4 54.20624 2 CGCGGAG 80 2.2885615E-7 41.50165 8 ACGCGGA 95 8.7993794E-7 34.94876 7 CCTTTAG 440 0.0 34.49488 1 CATTCCG 485 0.0 34.22817 9 CGCGGAC 35 1.9842292E-4 34.12851 92-93 CTTTAGG 445 0.0 34.107296 2 TATTCGA 30 0.0039299927 31.702318 94-95 GGCATTC 495 0.0 31.620308 7 TAGGCAT 520 0.0 31.012222 5 GCGGAGT 80 4.532134E-4 29.644037 9 ATGGGAC 390 0.0 29.187977 5 GGATGCC 115 3.908537E-6 28.870716 8 GCGCAGA 115 3.908537E-6 28.870716 1 TTAGGCA 580 0.0 28.621828 4 GGTATCA 7425 0.0 28.617975 1 GGGATTC 200 2.3646862E-11 28.458275 7 GTACAGG 85 6.46198E-4 27.900269 1 TGGGACC 120 5.442831E-6 27.66777 6 GTACGCA 70 0.008451472 27.10312 1 >>END_MODULE