##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939797_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1064796 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.394607042100084 31.0 31.0 34.0 30.0 34.0 2 31.611832689078472 31.0 31.0 34.0 30.0 34.0 3 31.635870157288345 31.0 31.0 34.0 30.0 34.0 4 35.47936881806468 37.0 35.0 37.0 33.0 37.0 5 35.236008587560434 37.0 35.0 37.0 33.0 37.0 6 35.26529213107487 37.0 35.0 37.0 32.0 37.0 7 35.190203569510025 37.0 35.0 37.0 32.0 37.0 8 35.14379280162585 37.0 35.0 37.0 32.0 37.0 9 36.695931427240524 39.0 35.0 39.0 32.0 39.0 10-11 36.57094974060759 38.5 35.0 39.0 32.0 39.0 12-13 36.55062378145673 38.0 35.0 39.0 32.0 39.0 14-15 37.48355365722636 39.0 36.0 41.0 32.0 41.0 16-17 37.415588525877254 39.0 36.0 41.0 32.0 41.0 18-19 37.538408765622705 39.0 36.0 41.0 32.0 41.0 20-21 37.52905720908043 39.0 36.0 41.0 32.0 41.0 22-23 37.37255915687136 39.0 36.0 41.0 32.0 41.0 24-25 37.21141232686824 39.0 36.0 40.5 31.0 41.0 26-27 36.904181176488265 39.0 36.0 40.0 31.0 41.0 28-29 36.68195222371234 38.5 35.0 40.0 30.0 41.0 30-31 36.357262330061346 38.0 35.0 40.0 30.0 41.0 32-33 36.19320790085612 38.0 35.0 40.0 30.0 41.0 34-35 36.14256439731179 38.0 35.0 40.0 30.0 41.0 36-37 35.98690359467917 38.0 35.0 40.0 30.0 41.0 38-39 35.801504231796514 38.0 35.0 40.0 29.5 41.0 40-41 35.61480133283746 38.0 35.0 40.0 28.5 41.0 42-43 35.340699533056096 38.0 34.5 40.0 27.0 41.0 44-45 34.969238708635274 38.0 34.0 40.0 25.5 41.0 46-47 34.511008211901625 38.0 33.5 40.0 24.0 41.0 48-49 34.10709938805179 38.0 33.0 40.0 23.0 41.0 50-51 33.853879992036035 38.0 33.0 40.0 20.5 41.0 52-53 33.611202521421944 38.0 33.0 40.0 19.0 41.0 54-55 33.322451906280634 38.0 33.0 40.0 15.0 41.0 56-57 32.13648295072483 37.5 31.5 40.0 5.5 41.0 58-59 29.094301631486218 35.0 23.0 39.0 2.0 40.0 60-61 29.04997623958016 34.5 23.0 39.0 2.0 40.0 62-63 30.82418932828448 35.5 29.0 39.0 2.0 40.0 64-65 30.429621260786103 36.0 29.0 39.0 2.0 40.0 66-67 25.13071987498075 29.0 17.5 36.0 2.0 39.0 68-69 17.52712867065616 13.0 7.0 29.5 2.0 36.5 70-71 16.496488059684673 13.5 7.5 26.5 2.0 34.0 72-73 18.640290252780815 18.5 7.0 31.0 2.0 35.5 74-75 19.284708056754532 18.0 7.5 31.5 2.0 36.0 76-77 20.14750712812595 23.5 7.5 31.5 2.0 35.5 78-79 23.103885626918206 29.0 9.5 34.0 2.0 36.0 80-81 25.528620036138378 31.5 19.0 35.0 2.0 37.0 82-83 26.371400719010964 33.0 24.0 35.0 2.0 37.0 84-85 26.292353652718454 33.0 24.0 35.0 2.0 36.0 86-87 25.980934376162196 33.0 22.5 35.0 2.0 36.0 88-89 25.515105710389598 33.0 19.5 35.0 2.0 35.5 90-91 23.945955375489767 31.5 6.0 34.5 2.0 35.0 92-93 22.015416568056228 30.5 2.0 34.0 2.0 35.0 94-95 17.824358374749714 20.0 2.0 33.0 2.0 35.0 96-97 16.60150254133186 13.5 2.0 32.5 2.0 34.5 98-99 15.55082804593556 2.0 2.0 32.0 2.0 34.0 100-101 14.90620175132138 2.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.36060060050489895 1101 2 0.5396361021499629 1101 3 0.44205957550225605 1101 4 0.2644820956560707 1101 5 0.4982563611523503 1101 6 0.5310149548304537 1101 7 0.30357330298931373 1101 8 0.4286211211674029 1101 9 0.45076669070439834 1101 10-11 0.3999387330830828 1101 12-13 0.5775652593649099 1101 14-15 0.89106759160709 1101 16-17 0.5568149352152147 1101 18-19 0.7318628634362696 1101 20-21 0.7287711726887949 1101 22-23 0.626923151605979 1101 24-25 0.6623459361883022 1101 26-27 0.7440453990395568 1101 28-29 0.7496774447983228 1101 30-31 0.8864272984570789 1101 32-33 0.840323064432333 1101 34-35 0.8859220604045319 1101 36-37 1.033918283263347 1101 38-39 0.7947626943545885 1101 40-41 0.9901253439798552 1101 42-43 1.0125099002708637 1101 44-45 0.5843979084994118 1101 46-47 0.5373638818599744 1101 48-49 0.8455765646741682 1101 50-51 0.7997390312706472 1101 52-53 0.8445585191748393 1101 54-55 1.0944441094237263 1101 56-57 1.7167665358059772 1101 58-59 -6.525108633663649 1101 60-61 -8.41085437949667 1101 62-63 -1.3515640219112477 1101 64-65 0.7788342800428687 1101 66-67 -0.7125582727976152 1101 68-69 -3.863288999443016 1101 70-71 -4.45563527324224 1101 72-73 -6.648311482983331 1101 74-75 -7.133155781581021 1101 76-77 -8.182884022337344 1101 78-79 -11.338626923718873 1101 80-81 -6.924936777696388 1101 82-83 -3.0731723155142454 1101 84-85 -1.9065860011500924 1101 86-87 -1.936089573556803 1101 88-89 -2.3575663702161265 1101 90-91 -8.085050683890815 1101 92-93 -14.177719178574769 1101 94-95 -12.099435775989205 1101 96-97 -12.343087911960936 1101 98-99 -11.796539308741977 1101 100-101 -11.322405301244448 1102 1 0.5184022658255714 1102 2 0.6094653928570324 1102 3 0.5487668076724646 1102 4 0.30164548087983434 1102 5 0.4670455226439003 1102 6 0.5275757056171599 1102 7 0.4692768870490198 1102 8 0.6106576579687584 1102 9 0.7034749254087913 1102 10-11 0.7156116841538491 1102 12-13 0.7122877523332463 1102 14-15 0.8126923931888683 1102 16-17 0.7584879141341503 1102 18-19 0.8280226005495237 1102 20-21 0.9554378857690793 1102 22-23 0.9197640828488431 1102 24-25 0.8993686881446337 1102 26-27 0.9518275706399919 1102 28-29 0.8752692184483806 1102 30-31 0.9092976095211611 1102 32-33 0.843992061062707 1102 34-35 0.9344405376665037 1102 36-37 1.0533145348994282 1102 38-39 1.0024473973538406 1102 40-41 1.181947032136037 1102 42-43 1.08726483635931 1102 44-45 1.2144767188255088 1102 46-47 1.3220975140970168 1102 48-49 1.2345633089444021 1102 50-51 1.1087806364392705 1102 52-53 1.0803977238310551 1102 54-55 0.7580451565149886 1102 56-57 1.481818472646328 1102 58-59 -8.071963182662508 1102 60-61 -8.954398477076666 1102 62-63 -2.049916106346906 1102 64-65 0.7248645231574109 1102 66-67 -0.8433089202676101 1102 68-69 -3.0149849531036583 1102 70-71 -3.8534707740916083 1102 72-73 -6.436379933789535 1102 74-75 -7.222162019571497 1102 76-77 -8.24517342053835 1102 78-79 -11.253362783809658 1102 80-81 -6.916060172945489 1102 82-83 -2.4597084206689424 1102 84-85 -1.5300265035311114 1102 86-87 -1.2319972847155647 1102 88-89 -1.3952379845746457 1102 90-91 -7.0450955194473615 1102 92-93 -13.775285248362287 1102 94-95 -11.934310041493674 1102 96-97 -12.072093871469693 1102 98-99 -11.458633073536319 1102 100-101 -11.222419921534627 1103 1 0.19570857576668033 1103 2 0.11935579154522102 1103 3 0.2241677722824562 1103 4 0.19529500838380187 1103 5 0.22514809851532647 1103 6 0.1850151583879196 1103 7 0.26101033488198055 1103 8 0.4331921836775052 1103 9 0.3014183739450331 1103 10-11 0.2968525923530265 1103 12-13 0.29192174835784357 1103 14-15 0.39572621376252926 1103 16-17 0.3557405708832704 1103 18-19 0.36688507514801216 1103 20-21 0.22636290825566618 1103 22-23 0.42909971974684424 1103 24-25 0.39466038457749875 1103 26-27 0.44979035949553037 1103 28-29 0.3635521312300156 1103 30-31 0.24590753841540902 1103 32-33 0.37335325679413955 1103 34-35 0.2590608566614705 1103 36-37 -0.03601044168861023 1103 38-39 -0.015509024927688131 1103 40-41 -0.07098455310387664 1103 42-43 0.1401666245522719 1103 44-45 0.11313992335909973 1103 46-47 -0.009358543587104862 1103 48-49 -0.023514281825548267 1103 50-51 0.16726428137691585 1103 52-53 0.07882921468280557 1103 54-55 -0.010854222003750635 1103 56-57 0.45412202497253773 1103 58-59 -6.3971054126661 1103 60-61 -8.495665999035793 1103 62-63 -2.5808999456131225 1103 64-65 -0.3544853830096564 1103 66-67 -2.049491529896681 1103 68-69 -3.9114707523122796 1103 70-71 -4.5023352474982055 1103 72-73 -7.416027717888646 1103 74-75 -8.090238833027177 1103 76-77 -8.632573896550802 1103 78-79 -11.878694067747755 1103 80-81 -8.072191146698177 1103 82-83 -4.257745054379956 1103 84-85 -3.201947136042836 1103 86-87 -3.010944838211447 1103 88-89 -3.3495295921715957 1103 90-91 -8.889800158532942 1103 92-93 -14.1678197000411 1103 94-95 -12.361715829153626 1103 96-97 -12.35743946169182 1103 98-99 -11.966086962143482 1103 100-101 -11.640519221581634 1104 1 0.1972684764345196 1104 2 0.23230192233256375 1104 3 0.2663325061510271 1104 4 0.2238296982922634 1104 5 0.17680403634432196 1104 6 0.20681760311632047 1104 7 0.17029740964630946 1104 8 0.25186920146246194 1104 9 0.2045365679642046 1104 10-11 0.3692461277260506 1104 12-13 0.2925389578956512 1104 14-15 0.43865964674948543 1104 16-17 0.35563288067745447 1104 18-19 0.5037066871257281 1104 20-21 0.2946971657959949 1104 22-23 0.35590011514670294 1104 24-25 0.4177362258067774 1104 26-27 0.34202139516991537 1104 28-29 0.2707292012527631 1104 30-31 0.4012876285858624 1104 32-33 0.4198079184138379 1104 34-35 0.33633018665158687 1104 36-37 0.3336900348807319 1104 38-39 0.33959460680395637 1104 40-41 0.2165008805902957 1104 42-43 0.11448251046495272 1104 44-45 0.2132206910134684 1104 46-47 0.36466601620908534 1104 48-49 0.2735543746989535 1104 50-51 0.17881727058378516 1104 52-53 0.20739484543398135 1104 54-55 0.09768946887714236 1104 56-57 1.1320969669082253 1104 58-59 -6.207042245127461 1104 60-61 -7.722230520738584 1104 62-63 -2.0523319038871826 1104 64-65 -0.14523005274629597 1104 66-67 -1.3654015523187475 1104 68-69 -3.851779839276137 1104 70-71 -4.488388160357543 1104 72-73 -7.541776928294414 1104 74-75 -8.343293939873544 1104 76-77 -8.760131739853517 1104 78-79 -11.766187538024454 1104 80-81 -7.803529800249141 1104 82-83 -3.987768119371797 1104 84-85 -3.1281275091009952 1104 86-87 -2.9061421764953117 1104 88-89 -3.004961920128455 1104 90-91 -8.425866331753443 1104 92-93 -13.641163159468414 1104 94-95 -11.903442971103782 1104 96-97 -11.846859300799437 1104 98-99 -11.29099634967553 1104 100-101 -10.809813850733534 1105 1 0.23845109551768928 1105 2 0.24056432838502673 1105 3 0.25288867558012385 1105 4 0.18831130697277132 1105 5 0.22329251377202297 1105 6 0.1348749422233837 1105 7 0.27103903524981376 1105 8 0.26967319329960304 1105 9 0.1821434566208211 1105 10-11 0.21937802758170477 1105 12-13 0.4312552985457003 1105 14-15 0.46645816349828095 1105 16-17 0.4328639791792739 1105 18-19 0.3756344159595386 1105 20-21 0.5124240086609575 1105 22-23 0.2973564886472744 1105 24-25 0.4422073820458934 1105 26-27 0.22734566972071235 1105 28-29 0.11868599782834366 1105 30-31 0.41412422208544086 1105 32-33 0.315238708319761 1105 34-35 0.37938677915569485 1105 36-37 0.15512998311626802 1105 38-39 0.2769969262504759 1105 40-41 0.3645845996684898 1105 42-43 0.41692639080938676 1105 44-45 0.6127329104151897 1105 46-47 0.522983173145029 1105 48-49 0.5533413439886843 1105 50-51 0.5328539039021862 1105 52-53 0.4720382180884002 1105 54-55 0.6199780558066124 1105 56-57 1.200256839487988 1105 58-59 -6.010075941807571 1105 60-61 -8.528106286006611 1105 62-63 -2.0637922592701905 1105 64-65 0.3108896028208754 1105 66-67 -1.18360373239938 1105 68-69 -3.4255765641778346 1105 70-71 -4.343843339293384 1105 72-73 -5.472101451712859 1105 74-75 -6.267929348763094 1105 76-77 -8.03355516327694 1105 78-79 -11.420935932828097 1105 80-81 -7.187000010791177 1105 82-83 -2.993840490085052 1105 84-85 -2.0706988096833463 1105 86-87 -1.8958365670184314 1105 88-89 -1.9035682612762308 1105 90-91 -7.068828354549719 1105 92-93 -10.306518293608079 1105 94-95 -11.581002574906481 1105 96-97 -12.068301737711488 1105 98-99 -11.409392646524864 1105 100-101 -11.005921560653398 1106 1 0.07685523890996748 1106 2 0.17719529776871923 1106 3 0.2032843614101516 1106 4 0.1343181075847184 1106 5 0.1676499880918385 1106 6 0.17036468285475337 1106 7 0.15283054075349156 1106 8 0.13862587533964899 1106 9 0.169335129174911 1106 10-11 0.2811824430662284 1106 12-13 0.2113658804944265 1106 14-15 0.3602079199536874 1106 16-17 0.18688013529177283 1106 18-19 0.14798799695442 1106 20-21 0.1158364283336013 1106 22-23 0.059813688333505866 1106 24-25 0.09474253294187918 1106 26-27 0.20096534371828056 1106 28-29 0.29086354903201084 1106 30-31 0.21513431796152815 1106 32-33 0.31014333202431743 1106 34-35 0.3733574623173226 1106 36-37 0.5344500405165462 1106 38-39 0.4061361253233926 1106 40-41 0.45102767969851243 1106 42-43 0.21331888133443755 1106 44-45 -0.04931318600982593 1106 46-47 -0.04655960683805915 1106 48-49 0.04580670983077795 1106 50-51 0.28088055742167484 1106 52-53 -0.12694423145339329 1106 54-55 -0.41355231568570616 1106 56-57 0.32070848551054 1106 58-59 -6.648842140699209 1106 60-61 -8.808080906542969 1106 62-63 -2.5302051313004306 1106 64-65 -0.5888839642474899 1106 66-67 -2.003321440928609 1106 68-69 -4.226120325116163 1106 70-71 -4.324292003483098 1106 72-73 -5.751419137200653 1106 74-75 -6.193801577225706 1106 76-77 -8.035395089679884 1106 78-79 -11.039562476900773 1106 80-81 -7.371657633694362 1106 82-83 -3.661782059715083 1106 84-85 -2.775840861828147 1106 86-87 -2.627549968832266 1106 88-89 -2.7392608004254946 1106 90-91 -7.91649058065736 1106 92-93 -10.659705805149974 1106 94-95 -10.963646274683352 1106 96-97 -11.440127822310831 1106 98-99 -11.032958274802127 1106 100-101 -10.878135622367173 1107 1 0.21589782006386216 1107 2 0.22539233417297666 1107 3 0.17674917281769353 1107 4 0.06645357216613945 1107 5 0.04254567083595617 1107 6 0.059544437093151714 1107 7 -0.04748733938843941 1107 8 0.09088142797468635 1107 9 0.013062951490589114 1107 10-11 0.2539026592575837 1107 12-13 0.11860073395742887 1107 14-15 0.3804791448189846 1107 16-17 0.3453006734702484 1107 18-19 0.28424481453885875 1107 20-21 0.2869027642515931 1107 22-23 0.2119932618648548 1107 24-25 0.20014854884409772 1107 26-27 0.3599670193655413 1107 28-29 0.48014844192200457 1107 30-31 0.3214734393966694 1107 32-33 0.2796896751705873 1107 34-35 0.4290908044122048 1107 36-37 0.30906809150879155 1107 38-39 0.4955027470870945 1107 40-41 0.47955187801629506 1107 42-43 0.4196707344031765 1107 44-45 0.47041181070459004 1107 46-47 0.4505170458101162 1107 48-49 0.46230115719573917 1107 50-51 0.4931834224499383 1107 52-53 0.4191869947261324 1107 54-55 0.4880892436519133 1107 56-57 1.1414639231501802 1107 58-59 -6.329737708447929 1107 60-61 -8.106500951884016 1107 62-63 -2.098780584710866 1107 64-65 0.1302364852202409 1107 66-67 -1.3828243322415261 1107 68-69 -3.431823275569574 1107 70-71 -4.173684170653553 1107 72-73 -5.411854148371635 1107 74-75 -5.965148830478434 1107 76-77 -8.673733734705511 1107 78-79 -11.703257196969112 1107 80-81 -7.397782535126879 1107 82-83 -3.2981123922160727 1107 84-85 -2.4043059196544085 1107 86-87 -2.127679338657476 1107 88-89 -2.1750190308105637 1107 90-91 -7.448650276412387 1107 92-93 -10.22268262536288 1107 94-95 -11.00666851807933 1107 96-97 -11.891023554338691 1107 98-99 -11.26674844105262 1107 100-101 -10.720017357812042 1108 1 -0.07465665108411912 1108 2 0.004884068953995069 1108 3 -4.950352731114549E-5 1108 4 -0.07274903244530861 1108 5 -0.09958635917502079 1108 6 -0.144309869360427 1108 7 -0.014933508718684152 1108 8 -0.14204067767371953 1108 9 0.0716179106219812 1108 10-11 -0.10818903587478701 1108 12-13 -0.11544220858252174 1108 14-15 -0.032680395256655004 1108 16-17 -0.10272718733248354 1108 18-19 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2312 84-85 0.15678734076105272 2312 86-87 0.18579801715081246 2312 88-89 0.2953110579674316 2312 90-91 1.8330548558842352 2312 92-93 3.6275599909769163 2312 94-95 7.595368878926589 2312 96-97 8.31523333611042 2312 98-99 8.534314501386746 2312 100-101 7.6488149649220905 2313 1 -0.2995231827743581 2313 2 -0.28230042836250036 2313 3 -0.27047764837436006 2313 4 -0.29782622915836754 2313 5 -0.2626163443012288 2313 6 -0.40201540207429787 2313 7 -0.4119918332389645 2313 8 -0.3352183828105524 2313 9 -0.5559058280742164 2313 10-11 -0.42952035398744925 2313 12-13 -0.29756530199337305 2313 14-15 -0.35992411913560574 2313 16-17 -0.4350180351390236 2313 18-19 -0.4030097975708884 2313 20-21 -0.41767978068691036 2313 22-23 -0.359515134540068 2313 24-25 -0.5645835940130439 2313 26-27 -0.7042773041916988 2313 28-29 -0.6767165060439382 2313 30-31 -0.7004015606033249 2313 32-33 -0.8873426095976242 2313 34-35 -0.9128912201857133 2313 36-37 -0.9250437817173207 2313 38-39 -1.0904155357889707 2313 40-41 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2314 4 -0.30380396552230593 2314 5 -0.47537578166464556 2314 6 -0.5122326028685578 2314 7 -0.5591154202690376 2314 8 -0.6687111779118879 2314 9 -0.7539088120892146 2314 10-11 -0.680043111261682 2314 12-13 -0.7041200126326643 2314 14-15 -0.8713589145253593 2314 16-17 -0.8787100049217145 2314 18-19 -0.660612657344366 2314 20-21 -0.7160480014818376 2314 22-23 -0.49667582763024853 2314 24-25 -0.5628038620197486 2314 26-27 -0.7097939880525033 2314 28-29 -0.8181181888509101 2314 30-31 -0.87366370465692 2314 32-33 -0.8972209194692766 2314 34-35 -1.0195392506248808 2314 36-37 -1.0231301018389019 2314 38-39 -0.9776719059399426 2314 40-41 -1.0176835551016694 2314 42-43 -1.2750394235293427 2314 44-45 -1.0602683759429397 2314 46-47 -1.2082096232494735 2314 48-49 -1.2314458516580586 2314 50-51 -1.02800286613585 2314 52-53 -1.0486013517682409 2314 54-55 -1.0800483191621595 2314 56-57 0.1977789805801855 2314 58-59 3.142690653600411 2314 60-61 3.0125967688625757 2314 62-63 0.5723002659711156 2314 64-65 0.17955151273878656 2314 66-67 1.1927948350233706 2314 68-69 0.08436032098462576 2314 70-71 2.7526797920169415 2314 72-73 7.573728526909534 2314 74-75 8.874703706013552 2314 76-77 7.759577165031267 2314 78-79 5.5323456685489525 2314 80-81 2.7865625534110485 2314 82-83 1.168078682203646 2314 84-85 0.7254851487830187 2314 86-87 0.5754980064011512 2314 88-89 0.9356601089357852 2314 90-91 1.9034038932454287 2314 92-93 3.8598961913396828 2314 94-95 7.538897107668458 2314 96-97 8.033544972820916 2314 98-99 8.43557343619074 2314 100-101 7.788934394999638 2315 1 -0.7481341185348 2315 2 -0.452663805960718 2315 3 -0.5933439814284078 2315 4 -0.3194541401388875 2315 5 -0.6986077706099465 2315 6 -0.7643801110623301 2315 7 -0.6089428357984445 2315 8 -0.7974789335009262 2315 9 -1.1122589103564025 2315 10-11 -0.9172466041739611 2315 12-13 -0.604573319025981 2315 14-15 -0.8776432245793799 2315 16-17 -0.8874495431675342 2315 18-19 -0.7900611708535692 2315 20-21 -0.6935271070036748 2315 22-23 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0.6592136072186499 2315 90-91 1.887583166271444 2315 92-93 3.867478150819341 2315 94-95 7.6252437745673625 2315 96-97 8.267142916322364 2315 98-99 8.571563176922126 2315 100-101 7.488381224757903 2316 1 -0.5864959331363409 2316 2 -0.4901382597850379 2316 3 -0.7001549490461052 2316 4 -0.43688424145393157 2316 5 -0.7162260905567734 2316 6 -0.43293828180085825 2316 7 -0.7672934069767692 2316 8 -0.5972202105301747 2316 9 -0.8202575810234904 2316 10-11 -0.670010871208369 2316 12-13 -0.7247428400313041 2316 14-15 -0.8308719816531109 2316 16-17 -0.8942266562788532 2316 18-19 -0.946094727191813 2316 20-21 -0.7449424738436505 2316 22-23 -0.87448107249255 2316 24-25 -2.095273558170746 2316 26-27 -1.7119575838090597 2316 28-29 -1.262398281068272 2316 30-31 -0.9779553240441885 2316 32-33 -1.154886937732634 2316 34-35 -1.3138175417219458 2316 36-37 -1.0710106335296103 2316 38-39 -1.2375825703732204 2316 40-41 -1.3756404542929772 2316 42-43 -1.5894722507478818 2316 44-45 -1.4923954089574707 2316 46-47 -1.4203770914146645 2316 48-49 -1.497021039424844 2316 50-51 -1.531528559116886 2316 52-53 -1.7706299535585543 2316 54-55 -1.5690232115452147 2316 56-57 -0.2559007081862248 2316 58-59 3.1461375475684576 2316 60-61 2.941900545811837 2316 62-63 0.22677785102902348 2316 64-65 -0.10140073956214124 2316 66-67 4.9888922576202255 2316 68-69 8.140971783314193 2316 70-71 5.694749009069952 2316 72-73 8.342818150655503 2316 74-75 8.782524620622436 2316 76-77 7.430154514142412 2316 78-79 5.092136108202418 2316 80-81 2.0870276927277054 2316 82-83 0.8237450066574574 2316 84-85 0.3843349533614635 2316 86-87 0.15866698750739872 2316 88-89 0.26494992720274624 2316 90-91 1.7992135868748633 2316 92-93 3.3888717141148454 2316 94-95 7.138969602760405 2316 96-97 7.829324947965457 2316 98-99 8.160970132936114 2316 100-101 7.364687263283708 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 8.0 5 37.0 6 141.0 7 397.0 8 776.0 9 1468.0 10 2708.0 11 4469.0 12 6300.0 13 8159.0 14 9922.0 15 13558.0 16 17498.0 17 21823.0 18 20836.0 19 18593.0 20 18729.0 21 18786.0 22 17916.0 23 17624.0 24 18070.0 25 18465.0 26 22721.0 27 33033.0 28 45315.0 29 59898.0 30 72487.0 31 79478.0 32 82932.0 33 88435.0 34 91601.0 35 92843.0 36 81904.0 37 56597.0 38 20303.0 39 959.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.40121570554037 25.738539706754015 16.165539604364966 20.694704983340646 2 13.87286765790109 24.354086893145983 41.36445475062762 20.40859069832531 3 15.19700288141133 31.475943784303634 34.3301188253459 18.996934508939137 4 10.102216762647492 21.678143043362297 43.05594686681768 25.163693327172528 5 9.129175864917519 41.986878571811204 37.745674355135655 11.138271208135624 6 22.73778263629841 45.015852801851246 19.46663961923223 12.779724942618117 7 21.94645734957682 37.71220027122566 25.372183967633237 14.969158411564282 8 22.967779743725558 37.41749593349336 22.432559851840164 17.182164470940915 9 25.099925243896482 17.460433735663923 24.755446113621765 32.68419490681783 10-11 20.52435396075868 29.9648477267007 31.300502631489973 18.210295681050642 12-13 22.283611133024543 29.474566020157848 30.416718319753265 17.82510452706434 14-15 20.611611834412884 30.229725402026496 29.127351513392746 20.031311250167875 16-17 16.942165447653824 33.38785081837272 30.657186916554906 19.012796817418547 18-19 16.926485352387015 31.512066800590922 34.32475786100616 17.236689986015904 20-21 17.22132690205448 31.158268813932434 34.19908602211128 17.42131826190181 22-23 17.82740543728564 30.925172521309246 33.348030984338784 17.89939105706633 24-25 17.514998178054764 32.24692805006781 33.45495287360208 16.78312089827535 26-27 17.14693975874195 31.303346611338256 34.694849282983036 16.854864346936754 28-29 16.690928557341024 30.502831597434188 35.433004310790125 17.373235534434667 30-31 17.3765543125334 30.987396437866476 34.620395060012264 17.015654189587856 32-33 16.761160865220624 31.4588201263721 34.61676083216253 17.16325817624475 34-35 17.249514339259562 31.15826506064831 34.23254333145659 17.359677268635544 36-37 17.99953593585018 30.367315229195086 33.63520988099667 17.997938953958073 38-39 16.6782289712054 31.685333095354963 33.82986268491272 17.806575248526922 40-41 16.411202802438513 30.858716497816115 35.363219309695666 17.36686139004971 42-43 16.766432751834213 30.666422418247237 34.52798992944978 18.039154900468766 44-45 16.75324254413303 30.83842306176581 34.08075582408508 18.32757857001608 46-47 17.51527284444574 30.878859076480243 33.042281095492505 18.563586983581516 48-49 16.829165756587212 30.906439135060488 33.27950360156604 18.98489150678626 50-51 16.585511344288168 30.58347756858122 33.16501060334563 19.666000483784984 52-53 16.740797798141006 31.34351912301384 33.29292521288238 18.62275786596277 54-55 17.13573184735363 30.727796603005302 32.6337393477 19.502732201941072 56-57 17.449995532166053 31.294213919889387 31.875643720812114 19.38014682713245 58-59 16.092352695954684 30.140966899206134 33.519705602477224 20.246974802361954 60-61 17.852124468947533 31.07215718823293 31.34588561816415 19.72983272465539 62-63 16.911268199546768 30.572749820857197 32.456543196658885 20.059438782937153 64-65 16.65292763614219 30.210787921252546 32.629735086725184 20.506549355880075 66-67 18.15318462676423 30.424786777870338 30.24727432413574 21.174754271229695 68-69 20.002220050433756 29.494142203882674 29.27112365922226 21.23251408646131 70-71 18.969102849681235 29.812197557416088 30.178729165066443 21.039970427836238 72-73 18.729224992967357 29.243746687002332 30.296825454538496 21.730202865491815 74-75 18.482316186115284 29.87011995898023 29.6886313228876 21.958932532016885 76-77 16.57020218834343 29.890278878672273 31.534694680071485 22.00482425291281 78-79 16.88130036519482 29.580733332327284 30.61892599523134 22.91904030724655 80-81 16.920017468151148 29.672284336421566 30.345700346076004 23.061997849351286 82-83 17.254666621587607 29.795660389407924 30.05298667538195 22.896686313622517 84-85 16.804157791727242 29.029598157769186 30.002883181379342 24.163360869124226 86-87 16.53987155038785 29.17877993999674 30.643736637441414 23.637611872173995 88-89 17.49400824195433 28.478600595794873 30.44616057911562 23.581230583135174 90-91 17.37695967920328 28.39474563587501 30.39393611363433 23.834358571287375 92-93 17.376499044619244 28.29371160954609 30.480785604279504 23.84900374155516 94-95 17.152442580820722 27.77961729744748 30.090466308721513 24.97747381301029 96-97 17.174107735252026 27.37133081225925 30.243329149510856 25.21123230297787 98-99 16.721094223943666 27.483236549515794 30.12160942405447 25.67405980248607 100-101 16.267768428268802 27.464878048550496 30.87728380498141 25.390069718199292 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1064.0 1 727.0 2 382.5 3 414.0 4 459.5 5 499.5 6 653.5 7 982.0 8 1357.5 9 1596.5 10 1663.5 11 1703.0 12 1858.0 13 1963.5 14 2036.5 15 2152.5 16 2266.0 17 2415.5 18 2617.5 19 2858.0 20 3202.5 21 3689.5 22 4235.5 23 4899.5 24 5801.0 25 7464.0 26 10296.0 27 13374.5 28 15484.0 29 17543.5 30 20877.0 31 25470.5 32 32024.0 33 39956.5 34 47857.5 35 55450.5 36 61896.5 37 65651.0 38 65309.0 39 62275.5 40 58097.0 41 53376.5 42 49233.0 43 46050.5 44 43567.0 45 40935.0 46 37410.0 47 32936.0 48 28652.5 49 23901.5 50 18497.0 51 13625.5 52 9224.0 53 5826.5 54 3577.5 55 2210.5 56 1482.0 57 1044.5 58 776.0 59 608.0 60 472.0 61 356.0 62 265.0 63 182.0 64 140.5 65 113.0 66 80.5 67 58.5 68 50.5 69 39.0 70 28.0 71 22.0 72 17.5 73 16.5 74 13.0 75 6.0 76 2.5 77 2.0 78 2.0 79 1.0 80 0.5 81 0.5 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021506466966442397 2 0.005916626283344415 3 0.0037565881164091527 4 0.0 5 0.06902730663901818 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.8174410873068646E-4 16-17 0.0 18-19 0.0014087205436534322 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.3478675727557204E-4 28-29 0.0024417822756659492 30-31 0.009109726182292194 32-33 3.756588116409153E-4 34-35 8.921896776471738E-4 36-37 0.0270474344381459 38-39 0.025685671245947582 40-41 0.027657880007062388 42-43 0.02873789909053002 44-45 0.029630088768177187 46-47 0.030522278445824367 48-49 0.030850979906010167 50-51 0.025685671245947582 52-53 0.022633443401365146 54-55 0.016763774469475842 56-57 0.1541140274756855 58-59 0.7634795773087052 60-61 0.3139098944774398 62-63 2.8174410873068646E-4 64-65 0.1430790498837336 66-67 1.4097066480339897 68-69 2.703193851216571 70-71 2.185958624938486 72-73 1.8473022062442008 74-75 1.5515648067798902 76-77 1.1260842452450985 78-79 0.42637275121243884 80-81 9.391470291022882E-5 82-83 0.0 84-85 0.0 86-87 0.0019252514096596905 88-89 0.0 90-91 0.22924578980386853 92-93 0.17552657973921765 94-95 1.295741156052427 96-97 1.2701493995093895 98-99 0.9994872257221102 100-101 0.4050071563003617 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1064796.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.68336463324736 #Duplication Level Percentage of deduplicated Percentage of total 1 73.91279203011905 13.070268525299744 2 9.817021228845087 3.471959320039955 3 3.8695988662415575 2.052825832084501 4 2.133945811142878 1.5094136754412129 5 1.37497896744671 1.2157127222203064 6 0.9823962634941558 1.0423242805024147 7 0.7070624798591864 0.8752270554886676 8 0.5867310716544679 0.8300303585377554 9 0.461741589165574 0.7348630398794951 >10 4.281194269277809 16.320366066909898 >50 0.88897704547865 11.199611861030757 >100 0.8826006410878526 30.922095367091956 >500 0.07864232081938723 9.434412236718876 >1k 0.020723314269973665 5.090476399823167 >5k 0.0015941010976902815 2.2304132589312875 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9668 0.9079673477360921 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8082 0.7590186289204692 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5987 0.5622673263235398 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3186 0.299212243471989 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 2355 0.22116912535358885 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 2268 0.21299854620039893 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 2220 0.20849064046070798 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1833 0.17214565043444943 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1781 0.1672620858831175 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1721 0.16162720370850378 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1575 0.14791565708361037 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1491 0.14002682203915115 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1483 0.13927550441586933 No Hit CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA 1440 0.1352371721907295 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1439 0.13514325748781927 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1436 0.13486151337908858 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 1434 0.13467368397326812 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1423 0.1336406222412556 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 1400 0.13148058407432034 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 1335 0.12537612838515547 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 1275 0.11974124621054175 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 1193 0.11204024057190297 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 1186 0.11138283765153138 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 1175 0.11034977591951886 No Hit CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT 1167 0.10959845829623702 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 1166 0.1095045435933268 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 1158 0.10875322597004497 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 1140 0.10706276131766085 No Hit CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG 1131 0.10621752899146877 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1107 0.1039635761216233 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 1096 0.10293051438961077 No Hit TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT 1096 0.10293051438961077 No Hit ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT 1088 0.10217919676632896 RNA PCR Primer, Index 27 (95% over 23bp) GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1075 0.10095830562849596 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 1067 0.10020698800521413 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 9.391470291022881E-5 0.0 12-13 0.0 0.0 0.0 2.8174410873068646E-4 0.0 14-15 0.0 0.0 0.0 2.8174410873068646E-4 0.0 16-17 0.0 0.0 0.0 4.2261616309602964E-4 0.0 18-19 0.0 0.0 0.0 5.165308660062585E-4 0.0 20-21 0.0 0.0 0.0 8.921896776471738E-4 0.0 22-23 0.0 0.0 0.0 0.0028174410873068644 0.0 24-25 0.0 0.0 0.0 0.00798274974736945 0.0 26-27 0.0 0.0 0.0 0.01873598323059065 0.0 28-29 0.0 0.0 0.0 0.04789649848421669 0.0 30-31 0.0 0.0 0.0 0.13016577823357714 0.0 32-33 0.0 0.0 0.0 0.2980852670370663 0.0 34-35 0.0 0.0 0.0 0.5598725013993291 0.0 36-37 0.0 0.0 0.0 0.9545020830281106 0.0 38-39 0.0 0.0 0.0 1.477513063535175 0.0 40-41 0.0 0.0 0.0 2.0388412428296125 0.0 42-43 0.0 0.0 0.0 2.5688019113520335 0.0 44-45 0.0 0.0 0.0 3.185492808012051 0.0 46-47 0.0 0.0 0.0 3.9002776118618026 0.0 48-49 0.0 0.0 0.0 4.55397090146845 0.0 50-51 0.0 0.0 0.0 5.177001040574908 0.0 52-53 0.0 0.0 0.0 5.736216139053866 0.0 54-55 0.0 0.0 0.0 6.325061326301 0.0 56-57 0.0 0.0 0.0 6.847743605347879 0.0 58-59 0.0 4.6957351455114406E-5 0.0 7.195134091412815 0.0 60-61 0.0 9.391470291022881E-5 0.0 7.450582083328637 0.0 62-63 0.0 9.391470291022881E-5 0.0 7.657194429731141 0.0 64-65 0.0 9.391470291022881E-5 0.0 7.868737298036431 0.0 66-67 0.0 9.391470291022881E-5 0.0 8.041681223445618 0.0 68-69 0.0 9.391470291022881E-5 0.0 8.23716467755326 0.0 70-71 0.0 9.391470291022881E-5 0.0 8.60019196165275 0.0 72-73 0.0 9.391470291022881E-5 0.0 9.020037641012927 9.391470291022881E-5 74-75 0.0 9.391470291022881E-5 0.0 9.510741963718871 9.391470291022881E-5 76-77 0.0 9.391470291022881E-5 0.0 10.20124981686633 9.391470291022881E-5 78-79 0.0 9.391470291022881E-5 0.0 11.1765070492376 9.391470291022881E-5 80-81 0.0 9.391470291022881E-5 0.0 12.228539551237983 9.391470291022881E-5 82-83 0.0 9.391470291022881E-5 0.0 13.158999470321076 9.391470291022881E-5 84-85 0.0 1.4087205436534323E-4 0.0 13.913885852313495 9.391470291022881E-5 86-87 0.0 1.8782940582045762E-4 0.0 14.607633762711355 9.391470291022881E-5 88-89 0.0 2.3478675727557204E-4 0.0 15.290393652868719 9.391470291022881E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGCCGA 15 0.009355484 48.701984 66-67 CCGTTGC 15 0.00949076 48.52534 70-71 CGTGAAC 80 1.8189894E-12 36.217106 74-75 GGCATTC 425 0.0 35.47811 7 GACAACG 35 1.7734138E-4 34.787132 64-65 GGTATCA 6620 0.0 33.769753 1 CTTTAGG 455 0.0 33.165375 2 CGTAAGT 30 0.003496556 32.46799 64-65 GCATGCG 30 0.003496556 32.46799 66-67 CTTACGG 45 2.0835643E-5 32.355705 62-63 CCTTTAG 445 0.0 31.79573 1 GATGGCC 120 1.6157537E-7 31.412912 9 CGGCGAG 40 3.8827604E-4 30.438744 66-67 GACATAT 435 0.0 30.358223 1 TAGACCC 125 2.2986569E-7 30.174818 5 ATCGCCC 40 4.208804E-4 30.02271 90-91 CATTCCG 505 0.0 29.85782 9 TGGGACG 80 4.7101747E-4 29.449608 8 TAGGCAT 535 0.0 28.200764 5 GTATCAA 9835 0.0 28.197496 1 >>END_MODULE