FastQCFastQC Report
Fri 27 May 2016
SRR939796_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939796_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1124866
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96220.8553907754345851No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73790.6559892467191648No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52100.463166279361275No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28450.25291901435370967No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA21390.19015598302375572No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT19010.16899790730629247No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17940.15948566318121446No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC16600.14757313315541584No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA14960.1329936187954832No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT14870.132193523495243No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA14700.13068223237256704No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14560.1294376396833045No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA13950.12401477153723198No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13910.12365917362601411No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA13860.12321467623699178No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA12900.1146803263677629No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT12780.11361353263410932No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA12780.11361353263410932No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC12650.11245783942265124No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11730.10427908746464025No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT11580.10294559529757323No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATTC4300.044.8672037
CTTTAGG4300.044.8652042
CCTTTAG4300.044.8632051
CATTCCG4400.043.8474929
TAGGCAT4650.043.512075
CAATCGC250.001686557337.6715286-87
TTAGGCA6250.033.1273164
GTCATGC350.003579581432.31262694-95
GTCAACG600.004137730731.3691653
GTATTGA902.5744761E-531.367771
ACTCCCG2500.030.1157426
CATGGGT7550.029.9162984
TACCTGG1901.4551915E-1129.7181572
GTATCAA111750.029.0099261
CATGGGG11550.028.9261024
AACGTGC455.651905E-428.54816690-91
GGATATG6300.028.3803631
TACGTCA400.00687812128.2727992-93
GGTATCA75400.028.2684331
AGGCATT7250.027.9089836
Produced by FastQC (version 0.11.5)