##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939796_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1124866 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.26122667055454 31.0 30.0 33.0 26.0 34.0 2 30.380137723071016 31.0 30.0 34.0 26.0 34.0 3 30.267992809810234 31.0 30.0 34.0 26.0 34.0 4 33.983474476070924 35.0 35.0 37.0 28.0 37.0 5 33.899515142248056 35.0 35.0 37.0 28.0 37.0 6 33.93001477509321 36.0 35.0 37.0 29.0 37.0 7 33.95629612771655 36.0 35.0 37.0 29.0 37.0 8 33.823352292628634 35.0 35.0 37.0 28.0 37.0 9 35.102893144605666 37.0 35.0 39.0 28.0 39.0 10-11 34.972690080418474 37.0 34.5 39.0 28.0 39.0 12-13 34.95349712765787 37.0 34.0 39.0 27.0 39.0 14-15 35.53394182062574 38.0 34.0 40.0 27.0 41.0 16-17 35.36387134111975 38.0 34.0 40.0 27.0 41.0 18-19 35.56024628711331 38.0 34.0 40.0 27.0 41.0 20-21 35.746948969921746 38.5 34.0 40.0 27.0 41.0 22-23 35.65804549164078 38.0 34.0 40.0 27.0 41.0 24-25 35.48045011583602 38.0 34.0 40.0 27.0 41.0 26-27 35.30545593875182 38.0 34.0 40.0 26.5 41.0 28-29 35.19965533672455 38.0 34.0 40.0 25.5 41.0 30-31 34.93725652655516 38.0 34.0 40.0 25.0 41.0 32-33 34.59516955797402 38.0 33.5 40.0 24.5 41.0 34-35 34.30853586116035 38.0 33.0 40.0 24.0 41.0 36-37 34.02974398728382 38.0 33.0 40.0 23.5 41.0 38-39 33.716494675810274 38.0 33.0 40.0 21.0 41.0 40-41 33.433659209185805 37.5 33.0 40.0 19.5 41.0 42-43 33.07917698641438 37.0 32.0 40.0 17.0 41.0 44-45 32.57138361369265 37.0 31.0 40.0 12.0 41.0 46-47 32.07201168850334 37.0 30.5 40.0 8.5 41.0 48-49 31.670033141725327 37.0 30.0 40.0 4.5 41.0 50-51 30.95298062169183 36.0 29.5 38.5 2.0 40.0 52-53 31.0830472251806 36.5 30.0 39.0 2.0 40.0 54-55 31.195752649648938 37.0 30.0 39.0 2.0 40.0 56-57 31.026800081076324 36.5 29.5 39.0 2.0 40.0 58-59 30.576830040200342 36.0 28.0 39.0 2.0 40.0 60-61 30.14165776190231 36.0 28.0 39.0 2.0 40.0 62-63 29.74291248913204 35.0 27.5 39.0 2.0 40.0 64-65 29.25283144836807 35.0 26.5 38.0 2.0 40.0 66-67 28.718149984086992 34.0 26.0 38.0 2.0 40.0 68-69 28.12958876879557 34.0 25.5 37.5 2.0 39.0 70-71 27.580080205108874 33.5 24.0 37.0 2.0 39.0 72-73 27.06078190646708 33.0 22.0 36.5 2.0 39.0 74-75 26.421603551000743 32.5 21.5 36.0 2.0 38.5 76-77 25.73396475669102 32.0 19.0 35.5 2.0 38.0 78-79 25.046294403066675 31.0 15.5 35.0 2.0 37.0 80-81 24.306321375168242 31.0 7.5 34.5 2.0 36.5 82-83 23.54019100941801 30.5 4.0 34.0 2.0 36.0 84-85 22.78249498162448 29.5 2.0 34.0 2.0 35.5 86-87 22.01076128178823 29.0 2.0 34.0 2.0 35.0 88-89 21.161605471229464 29.0 2.0 34.0 2.0 35.0 90-91 20.248157558322504 27.5 2.0 33.0 2.0 35.0 92-93 19.291525390579856 26.0 2.0 33.0 2.0 34.0 94-95 17.955328456900645 24.0 2.0 32.0 2.0 34.0 96-97 15.36726952365882 4.5 2.0 31.0 2.0 34.0 98-99 14.019501433948577 2.0 2.0 31.0 2.0 34.0 100-101 12.745122974647646 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6776861833803842 1101 2 0.6374446397197211 1101 3 0.7596485998739659 1101 4 0.7107458526308008 1101 5 0.7473808172923242 1101 6 0.7832788588737216 1101 7 0.6566745879408487 1101 8 0.6374999909103067 1101 9 0.7447875675150968 1101 10-11 0.7928211825847811 1101 12-13 0.41914548779105587 1101 14-15 0.7529305668551061 1101 16-17 0.8853275718630655 1101 18-19 0.7469895725515912 1101 20-21 0.8725838330621585 1101 22-23 0.9124270859374093 1101 24-25 0.7687694393885423 1101 26-27 0.9557317375775298 1101 28-29 0.9012534697871075 1101 30-31 0.979071089834342 1101 32-33 0.8884877880845679 1101 34-35 0.7731372361758062 1101 36-37 0.8370423872580872 1101 38-39 0.6940919175945837 1101 40-41 0.8592626655558533 1101 42-43 0.875834870638144 1101 44-45 0.9545284236070515 1101 46-47 0.8405200667757384 1101 48-49 0.7223880569518535 1101 50-51 0.9896973252554027 1101 52-53 1.2039652219213686 1101 54-55 1.0234246128370508 1101 56-57 0.935857327365099 1101 58-59 0.683449005049777 1101 60-61 0.7731299397319553 1101 62-63 0.7067872249159528 1101 64-65 0.7950679081332765 1101 66-67 0.6226263543843444 1101 68-69 0.491847231730155 1101 70-71 0.4369192067172847 1101 72-73 0.3565125696854565 1101 74-75 -0.007523288463538336 1101 76-77 -0.186681349064024 1101 78-79 -0.3714674187546976 1101 80-81 -0.6650947127867362 1101 82-83 -0.7733010175532762 1101 84-85 -0.850341475094698 1101 86-87 -0.923914952828575 1101 88-89 -1.146102335271273 1101 90-91 -1.3784258535969443 1101 92-93 -1.4508707240707537 1101 94-95 -1.1388806218470755 1101 96-97 0.5973080452845814 1101 98-99 0.4872511767214025 1101 100-101 -0.10261122110641274 1102 1 0.5028269881742204 1102 2 0.5719561908515338 1102 3 0.732160773928225 1102 4 0.6861843500226072 1102 5 0.7088545545210465 1102 6 0.6915013567299724 1102 7 0.5245138237389639 1102 8 0.5598355264392438 1102 9 0.8002653231427814 1102 10-11 0.5208806222527045 1102 12-13 0.5748309888395937 1102 14-15 1.0543151963613866 1102 16-17 0.9237663587490559 1102 18-19 0.7582774921710609 1102 20-21 0.7187404250958096 1102 22-23 0.7402608142851648 1102 24-25 0.9390928334215118 1102 26-27 0.9017634313068044 1102 28-29 0.8412187304848615 1102 30-31 0.8512299868379571 1102 32-33 0.8655949958621463 1102 34-35 0.880322654455675 1102 36-37 0.6293781659935291 1102 38-39 0.813019894736307 1102 40-41 0.8887734203825204 1102 42-43 0.5723629802097321 1102 44-45 0.7869226660462729 1102 46-47 0.7399800698981949 1102 48-49 0.5617886078537886 1102 50-51 0.7016278592034979 1102 52-53 0.8519034192277353 1102 54-55 0.715780161907098 1102 56-57 0.39135720693865395 1102 58-59 0.2772129550998734 1102 60-61 0.5794069425178456 1102 62-63 0.27903249945293496 1102 64-65 0.2384368829865089 1102 66-67 0.1811610587746948 1102 68-69 0.07065123632126458 1102 70-71 0.16023572742082592 1102 72-73 -0.0885867017149522 1102 74-75 -0.2754525106656267 1102 76-77 -0.19868986599865224 1102 78-79 -0.27115023041009323 1102 80-81 -0.5072105465496044 1102 82-83 -0.6406051990575001 1102 84-85 -0.7456857966544845 1102 86-87 -0.7601094929211669 1102 88-89 -0.7402275646503043 1102 90-91 -0.9525924594619859 1102 92-93 -0.9489701829755255 1102 94-95 -0.7345792403557176 1102 96-97 0.5304293966967872 1102 98-99 0.7182510613944508 1102 100-101 0.3502451607497541 1103 1 0.9007049722590992 1103 2 0.9468456691892868 1103 3 1.0168645758734058 1103 4 1.1813213968837886 1103 5 1.0996591522221948 1103 6 1.13005131252131 1103 7 1.0550178025188046 1103 8 0.9320724848831006 1103 9 1.3334218218165148 1103 10-11 1.2286613472748087 1103 12-13 1.0536108296883953 1103 14-15 1.2276131627628004 1103 16-17 1.2712464648498454 1103 18-19 1.1720705956193314 1103 20-21 1.1780684533893506 1103 22-23 1.2349557744973652 1103 24-25 1.1309584390802243 1103 26-27 1.432709496735633 1103 28-29 1.3969216482567433 1103 30-31 1.3998595182261013 1103 32-33 1.5716073742883268 1103 34-35 1.6853624422540818 1103 36-37 1.4671323658166955 1103 38-39 1.4628872687416177 1103 40-41 1.4546443310810204 1103 42-43 1.4874823436049667 1103 44-45 1.471272798672267 1103 46-47 1.513631705618792 1103 48-49 0.9503827723100251 1103 50-51 1.542476665569545 1103 52-53 1.6830263193161592 1103 54-55 1.6490250779106326 1103 56-57 1.469164457159696 1103 58-59 1.2900334679203844 1103 60-61 1.654119586196007 1103 62-63 1.2679803332283583 1103 64-65 1.3799046106611321 1103 66-67 1.2426111029833606 1103 68-69 0.6475743132443448 1103 70-71 0.9922428008072082 1103 72-73 0.7659667907695784 1103 74-75 0.5539020428268593 1103 76-77 0.4799131401905612 1103 78-79 0.36722289072164926 1103 80-81 0.3619318053513716 1103 82-83 0.42349736504506197 1103 84-85 0.6119623023729268 1103 86-87 0.3473175627817504 1103 88-89 0.27568755471309103 1103 90-91 0.17340223549822653 1103 92-93 0.2813569611447235 1103 94-95 0.375058248592115 1103 96-97 1.8522684771565583 1103 98-99 2.2297453142680137 1103 100-101 1.7817217301573596 1104 1 0.6408059185450021 1104 2 0.6988316379748518 1104 3 0.8002345153497288 1104 4 0.8061787545664814 1104 5 0.7979751175942269 1104 6 1.0015048310824852 1104 7 0.8954842020328542 1104 8 1.143478852603394 1104 9 1.1821977777766293 1104 10-11 0.9849252551383287 1104 12-13 0.8281933942166972 1104 14-15 0.9169840008572194 1104 16-17 0.9874500238944606 1104 18-19 0.9988380109063328 1104 20-21 0.79307218242635 1104 22-23 0.7022117126371654 1104 24-25 0.9413591452024548 1104 26-27 1.1893143777067436 1104 28-29 1.2098574153093864 1104 30-31 1.2290327515710686 1104 32-33 1.1077261956237336 1104 34-35 1.1523636959099477 1104 36-37 0.9979932830001417 1104 38-39 1.0208914698952256 1104 40-41 1.0805782058659616 1104 42-43 1.0722747909555963 1104 44-45 1.0176763116455945 1104 46-47 0.7937473855921553 1104 48-49 0.7475455871589709 1104 50-51 0.9718477165345725 1104 52-53 0.8642084810628496 1104 54-55 0.9679535328577096 1104 56-57 0.8821456112681396 1104 58-59 0.948215340010993 1104 60-61 1.1751880750351233 1104 62-63 1.126292512544481 1104 64-65 0.8424047912210284 1104 66-67 0.7290964799125916 1104 68-69 0.5035806476655971 1104 70-71 0.4796009472515479 1104 72-73 0.6315207523297026 1104 74-75 0.5760953247823508 1104 76-77 0.5138907112304238 1104 78-79 0.2090387378744687 1104 80-81 0.05597401303250038 1104 82-83 0.23676840331387794 1104 84-85 0.13830009914687125 1104 86-87 0.09145544820110274 1104 88-89 -0.1126549662679679 1104 90-91 -0.06152291387232367 1104 92-93 0.10144708143942793 1104 94-95 0.31372794410350835 1104 96-97 1.8120477151253755 1104 98-99 2.00130777696814 1104 100-101 1.5754843170826938 1105 1 0.7328240813031037 1105 2 0.9005514756643436 1105 3 0.7374658137160246 1105 4 0.7325905792051728 1105 5 0.8181211146024339 1105 6 0.8605873880030614 1105 7 0.8216089208163453 1105 8 0.9715370452793977 1105 9 0.8874585876801646 1105 10-11 1.0125010363607174 1105 12-13 0.7033476215927621 1105 14-15 0.8033386693456563 1105 16-17 1.0094596323704152 1105 18-19 1.0743508997507263 1105 20-21 0.976956120988639 1105 22-23 0.836865831060237 1105 24-25 0.8065618419362224 1105 26-27 0.9898575230912598 1105 28-29 0.9247186033092447 1105 30-31 0.9111944230866342 1105 32-33 0.8748829789780714 1105 34-35 0.9912012411107085 1105 36-37 1.1097098520998898 1105 38-39 1.109504851678281 1105 40-41 1.0394731133442576 1105 42-43 0.8996373040594037 1105 44-45 0.8953360897348475 1105 46-47 0.9393770455410362 1105 48-49 0.77693492036666 1105 50-51 0.8471311415454679 1105 52-53 0.7608021541332022 1105 54-55 0.9410657014344928 1105 56-57 0.6235681398020425 1105 58-59 0.7148976625354067 1105 60-61 0.6610517925111843 1105 62-63 0.8165016139473238 1105 64-65 0.8729708903505831 1105 66-67 0.4417215550018163 1105 68-69 0.5981658312366669 1105 70-71 0.6862579013970596 1105 72-73 0.6207038551931063 1105 74-75 0.682406942115886 1105 76-77 0.657636515067928 1105 78-79 0.3168477226681645 1105 80-81 0.5823895921323725 1105 82-83 0.341475908702634 1105 84-85 -0.07059223174041662 1105 86-87 0.047472671607735606 1105 88-89 0.22372193452002875 1105 90-91 0.2875245572404097 1105 92-93 0.2585963117012646 1105 94-95 0.4076432140124595 1105 96-97 1.9998701570858248 1105 98-99 2.2100028146010917 1105 100-101 1.567530748724998 1106 1 0.9100043949459469 1106 2 0.9509024643211319 1106 3 0.9103164924490557 1106 4 0.7472027096210851 1106 5 1.0018175752731082 1106 6 0.8985747431137057 1106 7 0.879847469133459 1106 8 0.9842075033467168 1106 9 1.2342903920562875 1106 10-11 1.0745915495944658 1106 12-13 1.0587910450106008 1106 14-15 1.2875341072679234 1106 16-17 1.000387776546944 1106 18-19 1.1535809716530352 1106 20-21 1.0067312713016676 1106 22-23 0.887730518347162 1106 24-25 1.172618604472639 1106 26-27 1.628319836362202 1106 28-29 1.397358533886326 1106 30-31 1.2770218420415702 1106 32-33 1.5345553120841302 1106 34-35 1.659565386031062 1106 36-37 1.5918164038881528 1106 38-39 1.4580815268161942 1106 40-41 1.4205100407601137 1106 42-43 1.3694972185204435 1106 44-45 1.658457486871157 1106 46-47 1.5490350446732535 1106 48-49 1.3088214262862046 1106 50-51 1.6095490117493938 1106 52-53 1.8731963926505308 1106 54-55 1.5575443476875073 1106 56-57 1.6503939781458108 1106 58-59 1.9501832572790967 1106 60-61 1.9720475000671307 1106 62-63 1.7469214056785596 1106 64-65 1.6867179460748751 1106 66-67 1.458947504918136 1106 68-69 1.7683627877853532 1106 70-71 1.9971993722952206 1106 72-73 1.977544259953536 1106 74-75 1.9394484099148066 1106 76-77 1.978430849661617 1106 78-79 1.688692698804033 1106 80-81 1.3691872274140913 1106 82-83 1.4638624766069306 1106 84-85 1.6600561287628999 1106 86-87 1.529296030561433 1106 88-89 1.4283374740972903 1106 90-91 1.2162378312749986 1106 92-93 0.8934077230940645 1106 94-95 1.0794843161013468 1106 96-97 2.7566009263165956 1106 98-99 3.1171744503786893 1106 100-101 2.849657445557577 1107 1 0.5999235906578413 1107 2 0.6883249263040376 1107 3 0.6460178882291494 1107 4 0.32334762860607213 1107 5 0.48765326172642176 1107 6 0.36515456464695717 1107 7 0.4522743947007939 1107 8 0.5343502231064079 1107 9 0.7406064598108131 1107 10-11 0.7573644125336827 1107 12-13 0.5438991480446873 1107 14-15 0.7505522566236777 1107 16-17 0.9070486705665033 1107 18-19 0.6647284385209815 1107 20-21 0.6120350823981724 1107 22-23 0.48273130378476736 1107 24-25 0.647120617874883 1107 26-27 0.832518287709128 1107 28-29 0.7734885103998295 1107 30-31 1.0333116646859324 1107 32-33 1.0793949870279036 1107 34-35 0.9816982514223795 1107 36-37 0.9090397095459863 1107 38-39 0.8853296982791434 1107 40-41 0.8362609382662569 1107 42-43 0.9682899434525325 1107 44-45 0.8361215571237608 1107 46-47 0.6084329013864433 1107 48-49 0.9297439514872572 1107 50-51 1.0164639277842014 1107 52-53 1.0816488506870847 1107 54-55 1.1760331657380156 1107 56-57 0.9251036535876693 1107 58-59 0.9164167199861346 1107 60-61 0.9673639931073801 1107 62-63 0.5588845782334495 1107 64-65 0.9659647688839819 1107 66-67 0.9676620309161237 1107 68-69 0.5650639498498684 1107 70-71 0.9394241147291602 1107 72-73 0.7460144956997574 1107 74-75 0.7688295233971338 1107 76-77 0.6476945689577107 1107 78-79 0.8170956551145458 1107 80-81 0.6402858055804579 1107 82-83 0.5599471659376256 1107 84-85 0.5213225606922762 1107 86-87 0.44764615182533873 1107 88-89 0.6791296166561338 1107 90-91 0.5850264566059344 1107 92-93 0.5857663730609239 1107 94-95 1.0972122175270123 1107 96-97 2.4761085144101234 1107 98-99 2.6011561209470013 1107 100-101 2.1697746098596298 1108 1 0.4169354341163185 1108 2 0.4789548209521328 1108 3 0.5014143115701017 1108 4 0.45117412321128114 1108 5 0.5126637096991473 1108 6 0.592203003265567 1108 7 0.4785618574969277 1108 8 0.3830228763100294 1108 9 0.4392774776795747 1108 10-11 0.390147061994206 1108 12-13 0.31443550153867506 1108 14-15 0.45827576147335236 1108 16-17 0.32460271838369437 1108 18-19 0.36912094796049644 1108 20-21 0.2226091576163114 1108 22-23 0.306340814892323 1108 24-25 0.3728807760299375 1108 26-27 0.5554206073407784 1108 28-29 0.47739659731242057 1108 30-31 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0.5456266203019098 2312 30-31 0.3863109920682106 2312 32-33 -0.530966625738067 2312 34-35 -0.02984577835815827 2312 36-37 0.34904684294475885 2312 38-39 0.40265241295863774 2312 40-41 0.655957614355188 2312 42-43 0.7886852151886927 2312 44-45 0.38800544842087703 2312 46-47 0.17485916689772552 2312 48-49 0.43478437173992646 2312 50-51 0.3904604425291325 2312 52-53 0.21238769106043875 2312 54-55 0.06307551537846834 2312 56-57 0.1102890728082464 2312 58-59 0.579948833620616 2312 60-61 0.48281980745927555 2312 62-63 0.47058138631834723 2312 64-65 0.39375665899594026 2312 66-67 0.66978997432075 2312 68-69 0.6669216525865345 2312 70-71 0.8866720691428078 2312 72-73 1.0237648913365902 2312 74-75 1.1316334125036747 2312 76-77 0.9989635556640337 2312 78-79 1.074892544222493 2312 80-81 0.8750778347094403 2312 82-83 1.194202856157144 2312 84-85 1.4077182705426878 2312 86-87 1.5469295822599562 2312 88-89 1.3865479301128403 2312 90-91 1.5006761224736067 2312 92-93 1.440777585696587 2312 94-95 2.080112772612157 2312 96-97 3.6152308536032383 2312 98-99 4.090196594559748 2312 100-101 3.599454463484534 2313 1 -0.02915210504450627 2313 2 -0.5254058389417509 2313 3 -0.7908042284780699 2313 4 -0.16376584527896654 2313 5 -0.3448651289672995 2313 6 0.03255721633668429 2313 7 0.010370408591619196 2313 8 0.23863215538864324 2313 9 0.1817558826342136 2313 10-11 -0.821473517133299 2313 12-13 0.045655508420111346 2313 14-15 0.10783118422919102 2313 16-17 -0.3162531154476369 2313 18-19 -0.11535959386235817 2313 20-21 -2.0301207820805445 2313 22-23 -0.23541322795369268 2313 24-25 -0.11902085559571418 2313 26-27 -0.04220348494295223 2313 28-29 -0.18253531264936385 2313 30-31 0.18917221338777068 2313 32-33 0.5788419264377609 2313 34-35 0.2790758581848749 2313 36-37 -0.4134549938627643 2313 38-39 0.25388884682306667 2313 40-41 0.41682521726888666 2313 42-43 0.2504869659286584 2313 44-45 0.5052451990348956 2313 46-47 0.6115701268658142 2313 48-49 0.3858896050669678 2313 50-51 0.6988152927506022 2313 52-53 0.791612873166784 2313 54-55 0.7688126524777807 2313 56-57 1.1535537620204117 2313 58-59 0.8620835188442904 2313 60-61 0.6332900301853073 2313 62-63 0.6886744652275958 2313 64-65 0.5695653791084574 2313 66-67 1.0481030502911075 2313 68-69 0.6826457745925403 2313 70-71 0.9922181782596731 2313 72-73 0.8266345094905461 2313 74-75 0.9543709904367965 2313 76-77 0.9722353821854384 2313 78-79 0.806719695386505 2313 80-81 0.9034465397076552 2313 82-83 0.8681716199332001 2313 84-85 0.8389418992346691 2313 86-87 0.8799480343035349 2313 88-89 1.0388287202549549 2313 90-91 1.3202701355306843 2313 92-93 1.387878578622253 2313 94-95 1.918579720572776 2313 96-97 3.425766781558396 2313 98-99 3.6528905089540213 2313 100-101 3.195167879526686 2314 1 -0.28345991583274355 2314 2 -0.4188315871005024 2314 3 -1.3490808872265632 2314 4 -0.39190247170396475 2314 5 -0.567728036129445 2314 6 -0.794273454104669 2314 7 -0.35938217566098274 2314 8 -2.1083312800131786 2314 9 -1.9994682883982975 2314 10-11 -1.2133036994681774 2314 12-13 -0.8500892878225557 2314 14-15 -0.8330504445278777 2314 16-17 -1.0064953247259965 2314 18-19 -2.363407710313723 2314 20-21 -1.7263612775628516 2314 22-23 -2.2942571748911007 2314 24-25 -2.290478558458183 2314 26-27 -2.303806860470381 2314 28-29 -3.396940961668271 2314 30-31 -1.9591318877912798 2314 32-33 -1.6448605238567282 2314 34-35 -1.8213211350792662 2314 36-37 -3.696104435794364 2314 38-39 -4.008071388769654 2314 40-41 -4.511974149277378 2314 42-43 -3.517281736684339 2314 44-45 -3.4393200065663976 2314 46-47 -3.3583722765704493 2314 48-49 -1.4613135448467993 2314 50-51 -1.637179847878297 2314 52-53 -2.840009918151427 2314 54-55 -2.3998476579579346 2314 56-57 -2.125236384736933 2314 58-59 -4.3032945467355255 2314 60-61 -2.7341597761991245 2314 62-63 -2.6204672266579045 2314 64-65 -1.8994452833062603 2314 66-67 -2.9519002037520394 2314 68-69 -2.48049784802523 2314 70-71 -1.9635008025740142 2314 72-73 -1.4623158446578195 2314 74-75 -1.769116693009888 2314 76-77 -0.9118881412924864 2314 78-79 -0.9013839567345912 2314 80-81 -0.7005103767610557 2314 82-83 -0.8964460842308917 2314 84-85 -0.9736013524221789 2314 86-87 -0.9177969137944757 2314 88-89 -0.6645739241974411 2314 90-91 -0.2870156670790571 2314 92-93 -0.015570707115273308 2314 94-95 0.6401670428206678 2314 96-97 2.1175329677113606 2314 98-99 2.0931712244096428 2314 100-101 1.7344116398989478 2315 1 -2.0207688050223 2315 2 -1.5864318216199642 2315 3 -1.237095589792748 2315 4 -1.5419137466780342 2315 5 -1.616972612866924 2315 6 -2.4317221529726645 2315 7 -1.7652902620723836 2315 8 -0.9931799698611883 2315 9 -0.5057191340213549 2315 10-11 -3.2102143565736725 2315 12-13 -1.2984983889816206 2315 14-15 -0.7709163174363667 2315 16-17 -1.5693510501578274 2315 18-19 -0.805210804166606 2315 20-21 -0.6560257343345981 2315 22-23 -0.6960949956641116 2315 24-25 -2.75781721357928 2315 26-27 -4.00863142187389 2315 28-29 -5.498569067923626 2315 30-31 -7.998263940287554 2315 32-33 -6.417343949644852 2315 34-35 -5.71974714869943 2315 36-37 -3.6791389823149068 2315 38-39 -6.6268295631258525 2315 40-41 -3.8860450914142106 2315 42-43 -3.7641754813287243 2315 44-45 -2.764069702142102 2315 46-47 -1.8191815942090201 2315 48-49 -2.4713167018395374 2315 50-51 -2.5150143763588844 2315 52-53 -1.3125621212813599 2315 54-55 -2.6678684490101148 2315 56-57 -2.4614640039134095 2315 58-59 -1.3999695568830042 2315 60-61 -2.2305562944871333 2315 62-63 -2.3965631541372723 2315 64-65 -1.6090211187818362 2315 66-67 -2.048615803806161 2315 68-69 -1.4679871367998665 2315 70-71 -1.7422643889177643 2315 72-73 -0.9567099275514259 2315 74-75 -1.3079319187348695 2315 76-77 -0.9465631965398593 2315 78-79 -0.6655543441426985 2315 80-81 -0.4689607443252122 2315 82-83 -0.16540044313586577 2315 84-85 -0.04886067682573625 2315 86-87 -0.5767808131315064 2315 88-89 -0.8964144509642296 2315 90-91 -0.8200862935166207 2315 92-93 -0.22840501009637748 2315 94-95 0.18786131062385536 2315 96-97 2.066317525506877 2315 98-99 1.5274685680257516 2315 100-101 1.3578816259179813 2316 1 -3.4999717336035943 2316 2 -2.375914715205635 2316 3 -2.588868394507948 2316 4 -3.2141152478061805 2316 5 -2.51271788155044 2316 6 -5.888813371833464 2316 7 -3.420224184805722 2316 8 -4.5754081781772555 2316 9 -6.641055506448485 2316 10-11 -5.802331131450497 2316 12-13 -3.3701622928357438 2316 14-15 -4.721291599473481 2316 16-17 -4.4664685157377875 2316 18-19 -2.500108810336883 2316 20-21 -3.3133195594485656 2316 22-23 -3.993417369841019 2316 24-25 -2.7744999966524446 2316 26-27 -3.7653116723688917 2316 28-29 -3.7514999515631757 2316 30-31 -3.4936788226377864 2316 32-33 -3.1365052058036227 2316 34-35 -5.383549699696346 2316 36-37 -5.711052920704187 2316 38-39 -5.332128407349273 2316 40-41 -4.572927487693626 2316 42-43 -3.6258333990890037 2316 44-45 -3.7009820288907314 2316 46-47 -3.663716981233012 2316 48-49 -3.8822067879471938 2316 50-51 -4.7825916199929 2316 52-53 -2.943652971767154 2316 54-55 -3.2917840450869207 2316 56-57 -2.5902306383472613 2316 58-59 -2.8233855443582527 2316 60-61 -3.0207483099588828 2316 62-63 -2.278044251894787 2316 64-65 -2.8848960094205047 2316 66-67 -3.576139172595589 2316 68-69 -3.0343913362749326 2316 70-71 -2.6135760341726346 2316 72-73 -2.5862950452011937 2316 74-75 -3.483999055079668 2316 76-77 -3.636660815916386 2316 78-79 -3.36898713872759 2316 80-81 -3.415953883340727 2316 82-83 -2.6094201051517985 2316 84-85 -2.4050551327819 2316 86-87 -2.2943822313752804 2316 88-89 -2.4302865327434837 2316 90-91 -1.820709077510724 2316 92-93 -1.9087901922117716 2316 94-95 -2.0972713043782214 2316 96-97 0.0994436478994718 2316 98-99 0.4781028637387852 2316 100-101 0.29609592807757856 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10594.0 3 2365.0 4 1880.0 5 2009.0 6 2331.0 7 3091.0 8 3711.0 9 4714.0 10 6156.0 11 8232.0 12 10697.0 13 12923.0 14 14886.0 15 17098.0 16 19541.0 17 20831.0 18 17080.0 19 15150.0 20 16013.0 21 17272.0 22 19183.0 23 21314.0 24 24020.0 25 27181.0 26 30762.0 27 34739.0 28 39423.0 29 44725.0 30 50420.0 31 57952.0 32 66317.0 33 77299.0 34 89658.0 35 104177.0 36 111274.0 37 95090.0 38 24595.0 39 163.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.65742676660463 24.764374092676704 15.340672916794615 20.23752622392405 2 14.311207551804733 23.746335263531712 41.76954036594852 20.172916818715034 3 15.733400508083223 31.525549251494073 33.73679722423489 19.004253016187807 4 10.354595786086982 20.905402842926822 43.55133497554926 25.188666395436933 5 9.539125217431558 41.38152049865491 37.58472486562418 11.494629418289353 6 22.911295721285324 44.50623504443938 19.797779455332726 12.784689778942571 7 22.424484148993848 37.69858828158873 25.519011503319412 14.357916066098014 8 23.22217750981991 36.800720779752645 22.528996784268198 17.448104926159246 9 25.997464270069482 17.472407910622643 24.767261304832488 31.762866514475384 10-11 20.835885583230652 29.65423542902727 31.32959458400701 18.18028440373507 12-13 22.677668968531975 29.50883598441801 30.306899052233334 17.506595994816685 14-15 21.21648724652621 30.0404774685514 29.14244205454978 19.600593230372613 16-17 17.243242665793048 33.18754239747601 30.634524409373114 18.934690527357834 18-19 17.147437538370035 31.4329546673766 34.46088738453375 16.95872040971962 20-21 17.608818503788157 30.962155836139992 34.45359290436957 16.975432755702276 22-23 18.171185203364214 30.617876061339082 33.56746671393874 17.643472021357955 24-25 17.707805702753816 31.912923969964872 33.76430310441684 16.614967222864475 26-27 17.287585586572916 31.12952223880976 34.72927044087359 16.85362173374373 28-29 16.95000126969305 30.30957789611416 35.59911914487732 17.141301689315465 30-31 17.55975425575044 30.842596263938947 34.623921304814644 16.973728175495967 32-33 17.09679833768752 31.43076846310942 34.59139867342902 16.88103452577404 34-35 17.557938337174733 31.014686396989234 34.34033692977475 17.087038336061283 36-37 18.32111142135337 30.20671485960944 33.60440193680232 17.867771782234875 38-39 17.053544387237 31.513557570682867 33.87954071725072 17.553357324829417 40-41 16.756959205585982 30.687528139875724 35.38950932072845 17.166003333809847 42-43 16.91719428660752 30.69690366477084 34.42433424584083 17.96156780278081 44-45 17.139852833371783 30.631849167121683 34.04005440586885 18.188243593637683 46-47 17.67095394410072 30.762867511946183 33.1454224964686 18.420756047484495 48-49 17.10937914214517 30.97062588356481 33.208047017120826 18.71194795716919 50-51 16.917154355286264 30.64963109751886 33.08794793789373 19.345266609301145 52-53 17.022140527318417 31.342703485236527 33.190421728150305 18.44473425929475 54-55 17.495320271691657 30.81886331627832 32.61160037794377 19.07421603408625 56-57 17.396362178237673 31.380077746466323 32.183606411298854 19.039953663997146 58-59 17.00972276920719 30.08044094611668 32.89574040776467 20.014095876911455 60-61 17.652285807057655 30.826270289964487 32.03884662717687 19.482597275800988 62-63 17.497739793441617 30.075844960964115 32.71883921135492 19.707576034239345 64-65 17.56472288341086 29.812850835101656 32.59552845981025 20.026897821677238 66-67 17.263250238241554 30.30722027769614 31.639992518769873 20.789536965292434 68-69 17.335000661319995 29.748444562012445 32.021114143831085 20.895440632836475 70-71 17.365518537503657 29.749521359246216 32.374374337819056 20.51058576543107 72-73 17.79221733890818 29.052283141131735 31.911360796220272 21.244138723739812 74-75 17.216609959844796 29.9026597252051 31.474068436727485 21.406661878222618 76-77 17.37343955278024 29.361408756508734 32.16852027801496 21.096631412696066 78-79 17.47852203051476 29.073907386282617 31.630053115642376 21.817517467560247 80-81 17.476433360575598 29.163766420000876 31.419274512023286 21.94052570740024 82-83 17.825070394543648 29.154236127295256 31.316047104076087 21.704646374085005 84-85 17.37718065187317 28.749206377494758 31.135309017111055 22.73830395352101 86-87 17.375096004604657 28.59792291476217 31.77142083250916 22.255560248124013 88-89 18.37694891272533 27.961342731090692 31.66904937030244 21.992658985881533 90-91 18.047649533524282 28.004670771635176 31.839033911209896 22.10864578363065 92-93 18.615990832440758 27.74942123544623 31.528900801804998 22.105687130308016 94-95 18.326336416966907 26.945509601563412 31.735374870450855 22.99277911101883 96-97 19.05824166204663 26.476272455817845 30.94778870376314 23.517697178372384 98-99 18.6594886198979 26.605720145344304 30.897122093671282 23.837669141086515 100-101 18.976919101591534 26.574268162557512 31.262504587151874 23.186308148699077 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2624.0 1 1608.5 2 623.0 3 663.0 4 569.0 5 562.0 6 871.5 7 1555.5 8 2328.5 9 2665.5 10 2367.5 11 1988.0 12 1925.0 13 1912.0 14 1949.0 15 2059.0 16 2169.0 17 2295.5 18 2443.5 19 2705.0 20 3138.0 21 3669.5 22 4290.0 23 5048.5 24 5822.0 25 7742.5 26 11996.0 27 15636.0 28 16341.0 29 18033.0 30 22440.0 31 27165.0 32 32957.5 33 40948.0 34 50818.0 35 58484.0 36 66306.5 37 74072.5 38 73392.5 39 67909.0 40 61674.5 41 55238.0 42 50417.5 43 47657.0 44 45601.5 45 42878.5 46 37316.5 47 31203.0 48 29412.0 49 25649.5 50 18299.5 51 12039.0 52 7615.0 53 4950.0 54 3178.5 55 2140.5 56 1603.0 57 1284.0 58 1100.5 59 956.0 60 783.5 61 664.0 62 551.0 63 397.5 64 309.0 65 251.5 66 192.5 67 162.0 68 140.0 69 107.5 70 85.0 71 64.5 72 45.0 73 32.0 74 20.0 75 14.5 76 11.5 77 7.0 78 5.0 79 5.5 80 5.5 81 4.5 82 3.0 83 1.5 84 1.0 85 1.0 86 1.5 87 1.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.12170338511431583 2 0.11734731070189694 3 0.1269484543047794 4 0.14108347127568974 5 0.13548280417400826 6 0.13672739686327084 7 0.14446165143225947 8 0.14561734464371756 9 0.15272930286807496 10-11 0.1488177258446784 12-13 0.14637299020505554 14-15 0.13557170365181276 16-17 0.13939438119740485 18-19 0.15615193276354694 20-21 0.18584435835023905 22-23 0.20326865599991467 24-25 0.22344883746152874 26-27 0.22558242492883596 28-29 0.22647141970688062 30-31 0.22767156265724092 32-33 0.22882725586869904 34-35 0.19811248628725553 36-37 0.18557765991682565 38-39 0.1996682271488337 40-41 0.18904473955119988 42-43 0.18984483485144008 44-45 0.19495655482519694 46-47 0.21660357767058477 48-49 0.23438347323147826 50-51 0.2508298766253047 52-53 0.27354369320434613 54-55 0.2672318302802289 56-57 0.23367227740904248 58-59 0.22687146735700073 60-61 0.20424655025576383 62-63 0.19388976109154335 64-65 0.1865555541726748 66-67 0.18277732636598493 68-69 0.18771124738413286 70-71 0.19162282440752942 72-73 0.22789381135175213 74-75 0.24180657962815125 76-77 0.258875279366609 78-79 0.2476739451632461 80-81 0.252030019575665 82-83 0.2486962891579975 84-85 0.23456127218708717 86-87 0.22438228197847565 88-89 0.22593802284005385 90-91 0.22824940926297 92-93 0.2354058172262296 94-95 0.24047308746108423 96-97 8.599779884892957 98-99 0.3014581292349489 100-101 0.3135929079552587 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1124866.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.540393309213144 #Duplication Level Percentage of deduplicated Percentage of total 1 81.98663873275251 21.759576380676197 2 7.378684715438312 3.916663889048245 3 2.709102892499984 2.1570196884622965 4 1.4582858737942581 1.5481392259107667 5 0.9438123870366926 1.2524575981030561 6 0.6058037382914307 0.9646961689467718 7 0.4737945380032511 0.8802285370454253 8 0.35695583563088157 0.7578998617329948 9 0.30531782330332524 0.7292929603304785 >10 2.6554696458331786 15.078151237902789 >50 0.5574208450865499 10.518208738978005 >100 0.5153319713168354 27.19220801432541 >500 0.042773237991105176 7.736832343456578 >1k 0.009581205310007557 3.491478201267601 >5k 0.001026557711786524 2.017147153813361 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9622 0.8553907754345851 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7379 0.6559892467191648 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5210 0.463166279361275 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2845 0.25291901435370967 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 2139 0.19015598302375572 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1901 0.16899790730629247 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1794 0.15948566318121446 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1660 0.14757313315541584 No Hit CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA 1496 0.1329936187954832 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1487 0.132193523495243 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1470 0.13068223237256704 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1456 0.1294376396833045 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1395 0.12401477153723198 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1391 0.12365917362601411 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 1386 0.12321467623699178 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1290 0.1146803263677629 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 1278 0.11361353263410932 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 1278 0.11361353263410932 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1265 0.11245783942265124 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1173 0.10427908746464025 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 1158 0.10294559529757323 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 8.889947780446738E-5 0.0 3 0.0 0.0 0.0 8.889947780446738E-5 0.0 4 0.0 0.0 0.0 8.889947780446738E-5 0.0 5 0.0 0.0 0.0 8.889947780446738E-5 0.0 6 0.0 0.0 0.0 8.889947780446738E-5 0.0 7 0.0 0.0 0.0 8.889947780446738E-5 0.0 8 0.0 0.0 0.0 8.889947780446738E-5 0.0 9 0.0 0.0 0.0 8.889947780446738E-5 0.0 10-11 0.0 0.0 0.0 1.7779895560893476E-4 0.0 12-13 0.0 0.0 0.0 1.7779895560893476E-4 0.0 14-15 0.0 0.0 0.0 2.666984334134021E-4 0.0 16-17 0.0 0.0 0.0 3.1114817231563585E-4 0.0 18-19 0.0 0.0 0.0 3.5559791121786953E-4 0.0 20-21 0.0 0.0 0.0 5.333968668268042E-4 0.0 22-23 0.0 0.0 0.0 0.0018668890338938149 0.0 24-25 0.0 0.0 0.0 0.0062674131852149505 0.0 26-27 0.0 0.0 0.0 0.01773544582199124 0.0 28-29 8.889947780446738E-5 0.0 0.0 0.04293844777955774 0.0 30-31 8.889947780446738E-5 0.0 0.0 0.12397032179832976 0.0 32-33 8.889947780446738E-5 0.0 0.0 0.28141129699004147 0.0 34-35 8.889947780446738E-5 0.0 0.0 0.5334857663046088 0.0 36-37 8.889947780446738E-5 0.0 0.0 0.9069524725611762 0.0 38-39 8.889947780446738E-5 0.0 0.0 1.4024781618432773 0.0 40-41 8.889947780446738E-5 0.0 0.0 1.9480098073903913 0.0 42-43 8.889947780446738E-5 0.0 0.0 2.4691829960190814 0.0 44-45 8.889947780446738E-5 0.0 0.0 3.0539637610168677 0.0 46-47 8.889947780446738E-5 0.0 0.0 3.7527581062988835 0.0 48-49 8.889947780446738E-5 0.0 0.0 4.449329964635788 0.0 50-51 8.889947780446738E-5 0.0 0.0 5.153502728324973 0.0 52-53 8.889947780446738E-5 0.0 0.0 5.798201741362972 0.0 54-55 8.889947780446738E-5 0.0 0.0 6.473082127115585 0.0 56-57 8.889947780446738E-5 0.0 0.0 7.2528194469385685 0.0 58-59 8.889947780446738E-5 0.0 0.0 8.068072108144436 0.0 60-61 8.889947780446738E-5 0.0 0.0 8.90870557026348 0.0 62-63 8.889947780446738E-5 0.0 0.0 9.66626247037425 0.0 64-65 8.889947780446738E-5 0.0 0.0 10.430753529753765 0.0 66-67 8.889947780446738E-5 0.0 0.0 11.27143144161171 0.0 68-69 8.889947780446738E-5 0.0 0.0 12.165137891980024 0.0 70-71 8.889947780446738E-5 0.0 0.0 13.148410566236333 0.0 72-73 8.889947780446738E-5 8.889947780446738E-5 0.0 14.056029784881044 0.0 74-75 8.889947780446738E-5 8.889947780446738E-5 0.0 14.959959675196867 0.0 76-77 8.889947780446738E-5 8.889947780446738E-5 0.0 15.90242748914093 0.0 78-79 8.889947780446738E-5 8.889947780446738E-5 0.0 16.904057905563864 0.0 80-81 8.889947780446738E-5 8.889947780446738E-5 0.0 17.935914144440314 0.0 82-83 8.889947780446738E-5 8.889947780446738E-5 0.0 18.914519596111894 0.0 84-85 8.889947780446738E-5 8.889947780446738E-5 0.0 19.86249917767983 0.0 86-87 8.889947780446738E-5 8.889947780446738E-5 0.0 20.730780377396066 0.0 88-89 8.889947780446738E-5 8.889947780446738E-5 0.0 21.559367960272603 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCATTC 430 0.0 44.867203 7 CTTTAGG 430 0.0 44.865204 2 CCTTTAG 430 0.0 44.863205 1 CATTCCG 440 0.0 43.847492 9 TAGGCAT 465 0.0 43.51207 5 CAATCGC 25 0.0016865573 37.67152 86-87 TTAGGCA 625 0.0 33.127316 4 GTCATGC 35 0.0035795814 32.312626 94-95 GTCAACG 60 0.0041377307 31.369165 3 GTATTGA 90 2.5744761E-5 31.36777 1 ACTCCCG 250 0.0 30.115742 6 CATGGGT 755 0.0 29.916298 4 TACCTGG 190 1.4551915E-11 29.718157 2 GTATCAA 11175 0.0 29.009926 1 CATGGGG 1155 0.0 28.926102 4 AACGTGC 45 5.651905E-4 28.548166 90-91 GGATATG 630 0.0 28.380363 1 TACGTCA 40 0.006878121 28.27279 92-93 GGTATCA 7540 0.0 28.268433 1 AGGCATT 725 0.0 27.908983 6 >>END_MODULE