##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939796_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1124866 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.357314560134274 31.0 31.0 34.0 30.0 34.0 2 31.59515889003668 31.0 31.0 34.0 30.0 34.0 3 31.598880222177574 31.0 31.0 34.0 30.0 34.0 4 35.446953681594074 37.0 35.0 37.0 33.0 37.0 5 35.207257575569 37.0 35.0 37.0 32.0 37.0 6 35.23541648516357 37.0 35.0 37.0 32.0 37.0 7 35.14144707013991 37.0 35.0 37.0 32.0 37.0 8 35.11890927452692 37.0 35.0 37.0 32.0 37.0 9 36.63812756363869 39.0 35.0 39.0 32.0 39.0 10-11 36.53287502689209 38.0 35.0 39.0 32.0 39.0 12-13 36.484956430365926 38.0 35.0 39.0 32.0 39.0 14-15 37.4048055501722 39.0 36.0 41.0 32.0 41.0 16-17 37.332219571042245 39.0 36.0 41.0 32.0 41.0 18-19 37.45213385416574 39.0 36.0 41.0 32.0 41.0 20-21 37.436584002005574 39.0 36.0 41.0 32.0 41.0 22-23 37.295905467851284 39.0 36.0 40.5 31.5 41.0 24-25 37.144146058286054 39.0 36.0 40.0 31.0 41.0 26-27 36.82552366237401 39.0 35.5 40.0 30.5 41.0 28-29 36.60156854238638 38.5 35.0 40.0 30.0 41.0 30-31 36.27058067360912 38.0 35.0 40.0 30.0 41.0 32-33 36.12343736942889 38.0 35.0 40.0 30.0 41.0 34-35 35.92488705321345 38.0 35.0 40.0 30.0 41.0 36-37 35.788996644933704 38.0 35.0 40.0 29.0 41.0 38-39 35.61972314924622 38.0 35.0 40.0 29.0 41.0 40-41 35.51348160580905 38.0 35.0 40.0 27.5 41.0 42-43 35.222462942252676 38.0 34.0 40.0 27.0 41.0 44-45 34.85173256192293 38.0 34.0 40.0 25.5 41.0 46-47 34.41121964749579 38.0 33.5 40.0 24.0 41.0 48-49 34.012777521944834 38.0 33.0 40.0 22.5 41.0 50-51 33.78998698511645 38.0 33.0 40.0 20.0 41.0 52-53 33.56664704951523 38.0 33.0 40.0 19.0 41.0 54-55 33.3063636024202 38.0 33.0 40.0 16.0 41.0 56-57 32.96974750770314 38.0 32.0 40.0 13.0 41.0 58-59 32.65005698456527 37.0 31.5 40.0 8.5 41.0 60-61 32.36062384319555 37.0 31.0 40.0 8.0 41.0 62-63 32.07868715029168 37.0 31.0 39.5 4.5 41.0 64-65 31.651186896928166 36.0 31.0 39.0 2.0 40.5 66-67 30.768186610671847 35.5 29.0 39.0 2.0 40.0 68-69 28.93130248402921 34.0 25.5 38.0 2.0 40.0 70-71 28.304557165031213 34.0 24.0 37.0 2.0 39.5 72-73 28.80121632265532 34.0 26.0 37.0 2.0 39.0 74-75 28.809203496238666 34.0 26.0 36.5 2.0 39.0 76-77 28.223499065666488 32.5 26.5 36.0 2.0 38.5 78-79 28.673616679675625 34.0 28.0 36.0 2.0 38.5 80-81 28.43058239825899 34.0 27.5 36.0 2.0 37.5 82-83 27.951121289113548 33.5 26.5 35.0 2.0 37.0 84-85 27.558684767785675 33.0 26.0 35.0 2.0 36.5 86-87 27.18251551740385 33.0 26.0 35.0 2.0 36.0 88-89 26.700118058506526 33.0 25.0 35.0 2.0 36.0 90-91 26.212615547096277 32.5 24.5 35.0 2.0 35.0 92-93 25.74970885421019 32.0 23.0 35.0 2.0 35.0 94-95 24.969739506750138 32.0 18.5 34.0 2.0 35.0 96-97 24.46007657801018 31.0 14.5 34.0 2.0 35.0 98-99 23.841079737497623 31.0 2.0 34.0 2.0 35.0 100-101 22.40664176888625 30.0 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2118212802953252 1101 2 0.2423585112027382 1101 3 0.32538903122167895 1101 4 0.13285503100632212 1101 5 0.2090662280065061 1101 6 0.12031036406871465 1101 7 0.17223779177692933 1101 8 0.2495958503996505 1101 9 0.33783748564032834 1101 10-11 0.37430296118003525 1101 12-13 0.465980855324176 1101 14-15 0.31383750495136553 1101 16-17 0.19831722111112526 1101 18-19 0.14735350497031874 1101 20-21 0.2973369213962158 1101 22-23 0.23085653312958954 1101 24-25 0.13804216233776145 1101 26-27 0.20912161295775178 1101 28-29 0.2424489860628185 1101 30-31 0.0926780480819005 1101 32-33 0.06096001591721745 1101 34-35 0.2586828453911423 1101 36-37 0.27043706010311297 1101 38-39 0.2512847405785621 1101 40-41 0.18413626099634683 1101 42-43 0.015267115479865367 1101 44-45 -0.28184911162252746 1101 46-47 -0.04716160290921323 1101 48-49 -0.00782966409178698 1101 50-51 0.14980962333157777 1101 52-53 0.04630118431885677 1101 54-55 -0.05056627580461992 1101 56-57 0.0813710358888784 1101 58-59 0.13215259249434297 1101 60-61 -0.04679131343901588 1101 62-63 -0.06982543994290324 1101 64-65 -0.22846655024588713 1101 66-67 -0.6659900862836743 1101 68-69 -8.685697075767678 1101 70-71 -5.971849094581163 1101 72-73 -1.4355488135482162 1101 74-75 -0.5450371399224458 1101 76-77 -0.6366061052860559 1101 78-79 -0.5268296408190061 1101 80-81 -0.474417587668313 1101 82-83 -0.6838218697256728 1101 84-85 -0.5225927597198066 1101 86-87 -0.3590871542853904 1101 88-89 -0.33762069418050444 1101 90-91 -0.8295795319795225 1101 92-93 -1.0931714418588392 1101 94-95 -0.5065405110340571 1101 96-97 -0.7813995771311397 1101 98-99 -1.0839236541027901 1101 100-101 -1.0146635437763578 1102 1 0.19830919803190383 1102 2 0.02103353789661 1102 3 0.28322800812255267 1102 4 0.2705083393599921 1102 5 0.2756820566698792 1102 6 0.3878437873099969 1102 7 0.31260402938026033 1102 8 -0.02398478471450005 1102 9 0.2566598640900324 1102 10-11 0.22568064753308903 1102 12-13 0.032491556017788525 1102 14-15 0.21050926326373087 1102 16-17 0.14427281474288378 1102 18-19 0.19478347915120509 1102 20-21 0.23331045739116263 1102 22-23 0.3249456738025813 1102 24-25 0.21572624023601605 1102 26-27 0.2780212290837554 1102 28-29 0.33943581446965965 1102 30-31 0.27271754167890805 1102 32-33 0.17402710874442562 1102 34-35 0.25988161806132126 1102 36-37 0.2932953326936669 1102 38-39 0.480743794411147 1102 40-41 0.49296300931762715 1102 42-43 0.4915176087575617 1102 44-45 0.5622570927321178 1102 46-47 0.45642883107316834 1102 48-49 0.4210022307928014 1102 50-51 0.5328904534031977 1102 52-53 0.2900050780548824 1102 54-55 0.1183665262401945 1102 56-57 0.20675418418914404 1102 58-59 0.18519279139018607 1102 60-61 0.4079581564100465 1102 62-63 0.5713304342393215 1102 64-65 0.5234840050886937 1102 66-67 0.7776719131718615 1102 68-69 -3.718560942107665 1102 70-71 0.3808615202840926 1102 72-73 0.6867860158973436 1102 74-75 0.6629012770543063 1102 76-77 0.22935847914072482 1102 78-79 0.1907283776599833 1102 80-81 0.27653739010182576 1102 82-83 0.3105808504213208 1102 84-85 0.17468144462229063 1102 86-87 -0.180959969839936 1102 88-89 -0.2056541029078538 1102 90-91 -0.04468547979054094 1102 92-93 -0.17970627491326852 1102 94-95 0.034113024050835605 1102 96-97 -0.5708600274397355 1102 98-99 -0.3030001441152379 1102 100-101 -0.5640340704439488 1103 1 0.2159926639912335 1103 2 0.22085670323701834 1103 3 0.25847115577949253 1103 4 0.30499109798068247 1103 5 0.28275544305360967 1103 6 0.23311346016588175 1103 7 0.05442542637407399 1103 8 0.3420629606435881 1103 9 0.31236278186192123 1103 10-11 0.5183420091599729 1103 12-13 0.40959146760045684 1103 14-15 0.4518885736085636 1103 16-17 0.4059881109409318 1103 18-19 0.33536703352786645 1103 20-21 0.48485776066261366 1103 22-23 0.5053170265877256 1103 24-25 0.4319066115888006 1103 26-27 0.3774907251049697 1103 28-29 0.43669487724596223 1103 30-31 0.3527352251448619 1103 32-33 0.4764143766489397 1103 34-35 0.4937454553734355 1103 36-37 0.6779107173090537 1103 38-39 0.7384803107683524 1103 40-41 0.7993290570629838 1103 42-43 0.6418270694118533 1103 44-45 0.7430705321662501 1103 46-47 0.6235375843888207 1103 48-49 0.6057944500677763 1103 50-51 0.7088409396985114 1103 52-53 0.7002614883112912 1103 54-55 0.689984563375468 1103 56-57 0.838938092235125 1103 58-59 0.6661830654839065 1103 60-61 0.7930156276744142 1103 62-63 0.680967923187147 1103 64-65 0.7378960298455475 1103 66-67 1.073869967990607 1103 68-69 -2.2388969279608943 1103 70-71 1.631966736906552 1103 72-73 1.4657426914057439 1103 74-75 1.0514954415105429 1103 76-77 0.9110560918728225 1103 78-79 0.9276868215149783 1103 80-81 0.9697292557074846 1103 82-83 0.4971414162922301 1103 84-85 0.676449791218225 1103 86-87 0.766431718931063 1103 88-89 0.49726366897543883 1103 90-91 0.3293198252492431 1103 92-93 0.29553687274367135 1103 94-95 0.12960374023120735 1103 96-97 0.355311502180065 1103 98-99 0.5882465402348913 1103 100-101 0.22111181815021652 1104 1 0.2672473461944129 1104 2 0.29664320313476367 1104 3 0.28656095372726753 1104 4 0.19835204367358727 1104 5 0.2934020147343688 1104 6 0.33546317161216166 1104 7 0.16087745864221858 1104 8 0.2480676605226293 1104 9 0.2576078985720329 1104 10-11 0.39981755222711257 1104 12-13 0.30434063379386345 1104 14-15 0.47825868110061975 1104 16-17 0.37233440733437106 1104 18-19 0.2954959875044736 1104 20-21 0.4519152729805924 1104 22-23 0.428391668908084 1104 24-25 0.5505496678634287 1104 26-27 0.369765802593669 1104 28-29 0.37893129591075336 1104 30-31 0.5399024722640746 1104 32-33 0.2835824904556503 1104 34-35 0.6088138058466583 1104 36-37 0.6029171604463741 1104 38-39 0.6538330715861704 1104 40-41 0.4894221347387102 1104 42-43 0.36157302353975496 1104 44-45 0.3154484413662928 1104 46-47 0.5006002436141443 1104 48-49 0.5330816497776709 1104 50-51 0.6419676364863705 1104 52-53 0.5176356076541637 1104 54-55 0.4125179821611553 1104 56-57 0.749707292594266 1104 58-59 0.6763453565190929 1104 60-61 0.6878560241307241 1104 62-63 0.827273135552197 1104 64-65 0.9375736068914016 1104 66-67 1.1560524695700316 1104 68-69 -3.3155520159866896 1104 70-71 1.2742741631259769 1104 72-73 1.6626852611949943 1104 74-75 1.2469707245714403 1104 76-77 0.9129669857545402 1104 78-79 0.527360445249446 1104 80-81 0.5801574069338109 1104 82-83 0.6652970368746907 1104 84-85 0.6193282428582059 1104 86-87 0.4901446689908546 1104 88-89 0.33201639314910025 1104 90-91 0.4743212051069712 1104 92-93 0.3712842196535888 1104 94-95 0.817459775835335 1104 96-97 0.5553004024980801 1104 98-99 0.6160079092362167 1104 100-101 0.6319034459489892 1105 1 0.23888276003910036 1105 2 0.1156332677998364 1105 3 0.2536748184371014 1105 4 0.07915673513049626 1105 5 0.2313356181925954 1105 6 0.17463236088411804 1105 7 0.09109969329751522 1105 8 0.26367812118765954 1105 9 0.2889745813362623 1105 10-11 0.2969773391503949 1105 12-13 0.193542435346302 1105 14-15 0.4235477157182217 1105 16-17 0.2413195396680905 1105 18-19 0.23925747173868217 1105 20-21 0.2726213472570862 1105 22-23 0.26649598992481316 1105 24-25 0.18069806478212058 1105 26-27 0.31291957943439286 1105 28-29 0.5310569098755877 1105 30-31 -0.002439383941528206 1105 32-33 0.04181064979793092 1105 34-35 0.3506323932269524 1105 36-37 0.07393897754668899 1105 38-39 0.3384520747546418 1105 40-41 0.18512195157990163 1105 42-43 0.17723450070673863 1105 44-45 0.28558427924424024 1105 46-47 0.3563041535541842 1105 48-49 0.05774477890757623 1105 50-51 -0.057630194465701834 1105 52-53 -0.07938519058846083 1105 54-55 -0.3242000592763148 1105 56-57 -0.26748556303418525 1105 58-59 -0.31294007381578126 1105 60-61 -0.3503684396438729 1105 62-63 -0.3746116039618137 1105 64-65 -0.39740935816119105 1105 66-67 0.05127245154861626 1105 68-69 1.3375412919439071 1105 70-71 1.686443696924961 1105 72-73 0.9020557190331324 1105 74-75 0.17677628765227738 1105 76-77 -0.08606163943539258 1105 78-79 -0.1368718336937711 1105 80-81 -0.1534558423388539 1105 82-83 -0.13460343309550993 1105 84-85 -0.3520443587036546 1105 86-87 -0.3313826016483752 1105 88-89 -0.15291975156403126 1105 90-91 0.1981007107548578 1105 92-93 0.2443271147406918 1105 94-95 0.3546848903832114 1105 96-97 0.17502796112656682 1105 98-99 0.2941166030959792 1105 100-101 0.24071730123585766 1106 1 0.3136782103583222 1106 2 0.3086499107056824 1106 3 0.2885226458715522 1106 4 0.22220127117462596 1106 5 0.37025739343588526 1106 6 0.32647175839233 1106 7 0.2662213383728229 1106 8 0.2810966112391071 1106 9 0.4165392004399635 1106 10-11 0.2899184381400417 1106 12-13 0.435908625763453 1106 14-15 0.615424226596545 1106 16-17 0.5654513657388733 1106 18-19 0.4817081163800978 1106 20-21 0.4598044502137597 1106 22-23 0.5226832303636684 1106 24-25 0.5482378125041762 1106 26-27 0.43799938480227496 1106 28-29 0.3616285642486119 1106 30-31 0.6176231431767931 1106 32-33 0.488938431363664 1106 34-35 0.7306674920926284 1106 36-37 0.636781055895419 1106 38-39 0.7907869628721684 1106 40-41 0.8985645072112121 1106 42-43 1.0077863974401495 1106 44-45 0.9654245135466013 1106 46-47 1.0206354155637314 1106 48-49 1.037827528551162 1106 50-51 1.1919324256297514 1106 52-53 1.1232603960972156 1106 54-55 1.163599791960273 1106 56-57 1.2592480729913547 1106 58-59 1.3335674728277525 1106 60-61 1.3513814673789994 1106 62-63 1.1846450439028118 1106 64-65 1.4158697126873854 1106 66-67 1.6522909385008226 1106 68-69 3.159714399859361 1106 70-71 3.5154482992927782 1106 72-73 2.239352718971144 1106 74-75 1.953170169757282 1106 76-77 1.3946312726082475 1106 78-79 1.2844195411280204 1106 80-81 1.3488263587396787 1106 82-83 1.0121671613382581 1106 84-85 1.145150056470218 1106 86-87 1.0877090892956538 1106 88-89 1.477629820741182 1106 90-91 1.1944625932317692 1106 92-93 1.2186293988787966 1106 94-95 1.5503506066170196 1106 96-97 1.340400179560831 1106 98-99 1.3203888401256734 1106 100-101 1.500593796617995 1107 1 0.06126308551205639 1107 2 0.10112197019970637 1107 3 0.16642579566791227 1107 4 0.11402314158891613 1107 5 0.14185520340596725 1107 6 0.0346491216974556 1107 7 0.10211238859868388 1107 8 0.11482368508173124 1107 9 0.14269733776510662 1107 10-11 0.23586960307811466 1107 12-13 0.17598816568859377 1107 14-15 0.15577886125311835 1107 16-17 0.3653984923782403 1107 18-19 0.1390423794672202 1107 20-21 0.31652568882970655 1107 22-23 0.08720712107673023 1107 24-25 0.4438163735058609 1107 26-27 0.39412905480353544 1107 28-29 0.21183373587630427 1107 30-31 0.31954765687597586 1107 32-33 0.3197363976549781 1107 34-35 0.15775436149159816 1107 36-37 0.3851801041749283 1107 38-39 0.4045409331634673 1107 40-41 0.43250307449640246 1107 42-43 0.3957757154887318 1107 44-45 0.39743588515053574 1107 46-47 0.306835468437086 1107 48-49 0.35848683636990586 1107 50-51 0.24851381803821226 1107 52-53 0.2067524133691805 1107 54-55 0.40527664377309947 1107 56-57 0.5182148968732534 1107 58-59 0.3564068489220915 1107 60-61 0.6721691271963905 1107 62-63 0.5703002712774179 1107 64-65 0.5096003880487778 1107 66-67 0.8899398363977689 1107 68-69 2.22748502142786 1107 70-71 2.4480171400251898 1107 72-73 1.618320232505578 1107 74-75 1.0873995405259755 1107 76-77 0.8322594809292845 1107 78-79 0.8927593559180025 1107 80-81 0.9175858654082347 1107 82-83 1.0797580901086477 1107 84-85 0.9825564265607518 1107 86-87 1.02616679635425 1107 88-89 1.057114190532868 1107 90-91 0.8098403120910831 1107 92-93 0.6553407524751407 1107 94-95 1.1568536468352555 1107 96-97 0.8628621624651132 1107 98-99 1.0960345273069478 1107 100-101 1.0373568741540957 1108 1 -0.12781869213327823 1108 2 -0.11603305591113866 1108 3 -0.1999320955496131 1108 4 -0.19245314787261947 1108 5 -0.09028675819637044 1108 6 0.019463038760534346 1108 7 -0.022470328455675315 1108 8 -0.0177750800587404 1108 9 -0.2503281407520461 1108 10-11 -0.12708969888828392 1108 12-13 -0.1849854763475065 1108 14-15 -0.1284030897377164 1108 16-17 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0.8228977349741484 2316 70-71 1.094785045095481 2316 72-73 -0.004277800064652837 2316 74-75 -0.39261946852888485 2316 76-77 -0.8036019233553837 2316 78-79 -0.6547977286589557 2316 80-81 -0.5708127587688239 2316 82-83 -0.5747423369654037 2316 84-85 -0.7229702746422397 2316 86-87 -1.0048899693061841 2316 88-89 -0.7798631623191916 2316 90-91 -0.7859568792210503 2316 92-93 -0.6592113235195924 2316 94-95 -0.46986807645484063 2316 96-97 -0.5154495134356871 2316 98-99 -1.1071975305825177 2316 100-101 -1.2783138173058326 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 8.0 5 55.0 6 144.0 7 414.0 8 870.0 9 1645.0 10 2890.0 11 4727.0 12 6447.0 13 8364.0 14 10082.0 15 12626.0 16 15816.0 17 18185.0 18 15574.0 19 11273.0 20 10768.0 21 10606.0 22 11196.0 23 11932.0 24 13896.0 25 15662.0 26 18560.0 27 22279.0 28 27508.0 29 33203.0 30 39525.0 31 46951.0 32 55257.0 33 67698.0 34 82824.0 35 105635.0 36 134913.0 37 172274.0 38 123240.0 39 11816.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.35624314226216 25.74437020847523 16.13437876230836 20.765007886954248 2 13.889647212191111 24.450072339596836 41.30595425576472 20.354326192447335 3 15.227516190831789 31.430349419708143 34.410618163068456 18.93151622639161 4 10.17054475822009 21.75672480099852 42.928935535432664 25.143794905348727 5 9.157890806549403 41.930594399688495 37.81321508517459 11.098299708587511 6 22.58313434666885 45.02883010065199 19.583665965546118 12.804369587133046 7 21.964038383238538 37.78414495593253 25.375200246073753 14.876616414755182 8 23.022297767022916 37.45992856037964 22.386310902809754 17.13146276978769 9 24.99444378263722 17.469991981267103 24.827312764364823 32.708251471730854 10-11 20.504531206383696 30.0027292139686 31.29110489604984 18.20163468359787 12-13 22.260473691977534 29.427238444401375 30.511545375182465 17.800742488438623 14-15 20.610366034709912 30.24057976683445 29.082486269475655 20.066567928979985 16-17 16.933195775657715 33.32596946379621 30.655774066407286 19.08506069413879 18-19 16.91748172671234 31.52771085622643 34.26350338618111 17.291304030880124 20-21 17.2371197991583 31.128374401928767 34.19073916359815 17.443766635314784 22-23 17.81541090227636 30.898435902587508 33.39722242471548 17.888930770420654 24-25 17.47266673215598 32.23785421457055 33.50356109241505 16.78591796085843 26-27 17.126641813236564 31.31007684057104 34.64715433855635 16.91612700763605 28-29 16.67760131215746 30.542776244260423 35.46692269739035 17.312699746191765 30-31 17.446866768427387 30.99334051051641 34.52768830993341 17.0321044111228 32-33 16.807863835522298 31.41693681487813 34.64551353941497 17.1296858101846 34-35 17.20132428664903 31.20448209154789 34.22284394469574 17.371349677107336 36-37 18.000500137493315 30.374142001671277 33.59406634030174 18.03129152053367 38-39 16.706101673800266 31.69248703049502 33.80765089562996 17.793760400074746 40-41 16.442892397014443 30.85637598704966 35.2812685495921 17.4194630663438 42-43 16.76743108794872 30.71207895979257 34.520658152529165 17.999831799729545 44-45 16.75899278654913 30.80026055988339 34.08689244963666 18.353854203930823 46-47 17.530106303603947 30.850513365476978 33.00465959755635 18.614720733362727 48-49 16.845362567739524 30.942480356650265 33.199975084312904 19.012181991297304 50-51 16.61025138613412 30.584156204479584 33.127426457698775 19.67816595168752 52-53 16.76129122373729 31.302171212100316 33.312841107701175 18.62369645646121 54-55 17.14302870181338 30.740429393005353 32.58089481339151 19.53564709178976 56-57 17.218651412106563 31.34807654255662 32.03368690652728 19.39958513880953 58-59 16.503970416815967 30.340813004082 32.75689454811224 20.39832203098979 60-61 17.342259240127284 31.166508556192046 31.691384949957367 19.7998472537233 62-63 16.96217588666101 30.591738543683622 32.384218883074716 20.061866686580647 64-65 16.94408795925652 30.29680559142634 32.20848982502121 20.550616624295927 66-67 16.743252206972386 30.79197254139047 31.194191311006147 21.270583940630996 68-69 17.025922285810182 30.057987936438224 31.526968050396658 21.389121727354933 70-71 17.28696474830257 30.17941572191344 31.48921073593692 21.044408793847076 72-73 17.57370972633075 29.39652228046278 31.191445570514237 21.838322422692233 74-75 17.01198836467892 30.300878518319585 30.466728975453243 22.220404141548254 76-77 16.809544855896082 29.899928946261493 31.287033092900764 22.00349310494166 78-79 16.978885967867978 29.5410638541888 30.582451766852152 22.897598411091067 80-81 17.018346625605975 29.640064044721065 30.24770656067224 23.09388276900072 82-83 17.23606066680861 29.825299113538463 30.090708622497537 22.847931597155394 84-85 16.802579876606867 29.074951549526162 29.99328805362445 24.129180520242517 86-87 16.585382115917163 29.156335705490932 30.62163567748732 23.636646501104586 88-89 17.503998264677563 28.548409895249467 30.381744265746143 23.565847574326828 90-91 17.21552926973352 28.372152358989787 30.60794865833997 23.804369712936715 92-93 17.52542720957017 28.24515026068418 30.366229162934616 23.863193366811032 94-95 17.103681566189273 27.214220192873178 30.730699114107285 24.95139912683026 96-97 17.621121284669197 26.98734492363163 30.188081154317242 25.203452637381936 98-99 17.070882707176743 27.08483846400424 30.23682105732086 25.607457771498154 100-101 17.029531048076805 27.081352304972345 30.59010083739986 25.29901580955099 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1756.0 1 1098.5 2 531.5 3 656.0 4 568.5 5 575.0 6 901.0 7 1645.5 8 2469.0 9 2745.0 10 2427.5 11 2036.0 12 1998.5 13 1975.0 14 1942.0 15 1974.5 16 2048.5 17 2277.0 18 2524.5 19 2729.0 20 3049.5 21 3504.0 22 4036.5 23 4591.0 24 5198.0 25 6935.5 26 11215.0 27 15392.5 28 15918.5 29 17017.0 30 21544.0 31 25974.5 32 31961.5 33 40798.0 34 50745.5 35 58422.0 36 66461.0 37 73538.0 38 71994.0 39 66619.0 40 60332.0 41 53408.0 42 48168.5 43 46432.0 44 45616.5 45 43930.5 46 40091.0 47 34287.5 48 32382.0 49 28079.0 50 20463.5 51 15191.5 52 10282.5 53 5995.0 54 3282.0 55 1902.0 56 1232.0 57 946.0 58 801.0 59 729.5 60 578.5 61 448.5 62 348.5 63 225.5 64 159.5 65 137.5 66 110.0 67 89.5 68 78.0 69 63.5 70 49.0 71 32.0 72 21.5 73 16.0 74 11.0 75 8.0 76 8.0 77 6.5 78 4.0 79 1.5 80 1.0 81 0.0 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01991348302820069 2 0.02746993864158042 3 8.889947780446738E-5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.0004765012010315E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.444973890223369E-5 26-27 4.0004765012010315E-4 28-29 8.889947780446738E-5 30-31 4.444973890223369E-4 32-33 0.0018224392949915812 34-35 0.003022582245351891 36-37 0.10445688642024917 38-39 0.09565583811760689 40-41 0.10570147910951172 42-43 0.10716832049328542 44-45 0.10063420887465707 46-47 0.09392229830041979 48-49 0.09565583811760689 50-51 0.09396674803932202 52-53 0.07667579960635311 54-55 0.06409652349702098 56-57 0.03498194451605791 58-59 0.015379609660172856 60-61 0.011112434725558422 62-63 0.0024891853785250864 64-65 0.006445212140823885 66-67 0.15299600130148835 68-69 0.41187128066809736 70-71 0.39324684006806143 72-73 0.18068818863757993 74-75 0.031914912531803785 76-77 0.03244830939863059 78-79 0.006134063968508249 80-81 0.002400285900720619 82-83 4.444973890223369E-5 84-85 5.333968668268042E-4 86-87 9.778942558491411E-4 88-89 2.666984334134021E-4 90-91 8.4454503914244E-4 92-93 0.0033337304176675265 94-95 0.08134302219108765 96-97 0.019424535900276124 98-99 0.021380324411974405 100-101 0.011956979764700861 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1124866.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.672681427517595 #Duplication Level Percentage of deduplicated Percentage of total 1 74.14348048473435 13.103141105340779 2 9.78607102557584 3.458922313241243 3 3.924476491318987 2.0806806840258747 4 2.107645211664697 1.4899096955193232 5 1.3594902406680403 1.2012918963572752 6 0.9540678162429751 1.0116561946025695 7 0.6943175981337473 0.858931760493583 8 0.5412766340248424 0.7652647613824055 9 0.4622355672394978 0.7352047731862379 >10 4.16229015119804 15.781311104036869 >50 0.8873537440222478 11.13824798078062 >100 0.8762995071199462 31.215900772322165 >500 0.07788212362591265 9.528941997010586 >1k 0.02160600848976932 5.436369344593511 >5k 0.0015073959411466965 2.1942256171069676 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9967 0.8860610952771263 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8479 0.7537786723040789 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6264 0.5568663289671837 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3273 0.2909679908540217 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 2572 0.22864945691309008 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 2436 0.21655912793168255 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 2354 0.2092693707517162 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1983 0.1762876644862588 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1908 0.16962020365092376 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1773 0.15761877414732064 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 1618 0.1438393550876282 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1615 0.14357265665421481 No Hit CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA 1580 0.14046117493105845 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1569 0.13948328067520932 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1536 0.13654959790766188 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1531 0.13610510051863955 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1510 0.13423821148474574 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 1503 0.13361591514011445 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 1483 0.13183792558402513 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 1374 0.12214788250333816 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 1306 0.1161027180126344 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 1270 0.11290233681167357 No Hit CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT 1259 0.11192444255582441 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 1257 0.11174664360021548 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 1257 0.11174664360021548 No Hit CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG 1219 0.10836846344364573 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 1217 0.1081906644880368 No Hit TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT 1205 0.10712387075438319 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 1201 0.10676827284316533 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 1186 0.10543478067609831 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 1168 0.1038345900756179 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 1144 0.10170100260831069 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1133 0.10072310835246154 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 8.889947780446738E-5 0.0 3 0.0 0.0 0.0 8.889947780446738E-5 0.0 4 0.0 0.0 0.0 8.889947780446738E-5 0.0 5 0.0 0.0 0.0 8.889947780446738E-5 0.0 6 0.0 0.0 0.0 8.889947780446738E-5 0.0 7 0.0 0.0 0.0 8.889947780446738E-5 0.0 8 0.0 0.0 0.0 8.889947780446738E-5 0.0 9 0.0 0.0 0.0 8.889947780446738E-5 0.0 10-11 0.0 0.0 0.0 1.7779895560893476E-4 0.0 12-13 0.0 0.0 0.0 1.7779895560893476E-4 0.0 14-15 0.0 0.0 0.0 2.666984334134021E-4 0.0 16-17 0.0 0.0 0.0 3.1114817231563585E-4 0.0 18-19 0.0 0.0 0.0 3.5559791121786953E-4 0.0 20-21 0.0 0.0 0.0 5.77846605729038E-4 0.0 22-23 0.0 0.0 0.0 0.0020891377284049835 0.0 24-25 0.0 0.0 0.0 0.0068452597909439885 0.0 26-27 0.0 0.0 0.0 0.01853554112223145 0.0 28-29 0.0 0.0 0.0 0.04458308811894039 0.0 30-31 0.0 0.0 0.0 0.12823749673294418 0.0 32-33 0.0 0.0 0.0 0.29314602806023116 0.0 34-35 0.0 0.0 0.0 0.5563773818392591 0.0 36-37 0.0 0.0 0.0 0.9466905391397731 0.0 38-39 0.0 0.0 0.0 1.4630187062281197 0.0 40-41 0.0 0.0 0.0 2.028952781931359 0.0 42-43 0.0 0.0 0.0 2.5720841415777524 0.0 44-45 0.0 0.0 0.0 3.180867765582745 0.0 46-47 0.0 0.0 0.0 3.903887218566478 0.0 48-49 0.0 0.0 0.0 4.622950644787912 0.0 50-51 0.0 0.0 0.0 5.353882151296244 0.0 52-53 0.0 0.0 0.0 6.020717134307553 0.0 54-55 0.0 0.0 0.0 6.715110955438248 0.0 56-57 0.0 0.0 0.0 7.510494583354817 0.0 58-59 0.0 0.0 0.0 8.310856582028437 0.0 60-61 0.0 0.0 0.0 9.09254969036312 0.0 62-63 0.0 0.0 0.0 9.78885485026661 0.0 64-65 0.0 0.0 0.0 10.512896647245093 0.0 66-67 0.0 0.0 0.0 11.27200928821744 0.0 68-69 0.0 0.0 0.0 12.09206252122475 0.0 70-71 0.0 0.0 0.0 13.054310468980304 0.0 72-73 0.0 0.0 0.0 13.97059738671095 0.0 74-75 0.0 0.0 0.0 14.902308363840671 0.0 76-77 0.0 0.0 0.0 15.882736254807238 0.0 78-79 0.0 0.0 0.0 16.930638849427396 0.0 80-81 0.0 0.0 0.0 18.01503467968629 0.0 82-83 0.0 0.0 0.0 19.042357045194716 8.889947780446738E-5 84-85 0.0 0.0 0.0 20.037764498171338 8.889947780446738E-5 86-87 0.0 0.0 0.0 21.077888388483608 8.889947780446738E-5 88-89 0.0 0.0 0.0 22.12761342239876 8.889947780446738E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTATCA 15 6.647617E-4 94.89294 2 CGTCAGA 35 2.803487E-4 54.224533 2 GGCATTC 410 0.0 39.335358 7 ACACGGC 25 0.0016159139 38.001106 92-93 CTTTAGG 420 0.0 36.14969 2 CATTCCG 435 0.0 35.984272 9 TAGGCAT 440 0.0 35.57536 5 CCTTTAG 415 0.0 35.451397 1 TGACAGC 30 0.003843031 31.84763 62-63 ATGTATT 105 1.9144427E-6 31.639418 1 GGACCGA 30 0.003977948 31.623947 20-21 GGTATCA 7585 0.0 29.720613 1 TTAGGCA 575 0.0 29.697691 4 GTTCGCA 245 0.0 29.041111 9 CTGTCGC 75 3.1597665E-8 28.662865 62-63 GTACATG 6590 0.0 27.582466 1 CATGGGG 1085 0.0 27.542213 4 GCAGATC 190 4.0199666E-10 27.461681 8 TGTTCGC 260 0.0 27.365662 8 TCGCCGA 35 0.008086771 27.350615 66-67 >>END_MODULE