##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939795_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 155132 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.16988757960962 31.0 28.0 33.0 16.0 34.0 2 28.716054714694582 31.0 28.0 33.0 16.0 34.0 3 28.9255795064848 31.0 28.0 33.0 22.0 34.0 4 32.44418301833277 35.0 33.0 37.0 25.0 37.0 5 32.57609003945027 35.0 33.0 37.0 25.0 37.0 6 32.80543021426914 35.0 33.0 37.0 26.0 37.0 7 32.778556326225406 35.0 33.0 37.0 26.0 37.0 8 32.726007529072014 35.0 33.0 37.0 25.0 37.0 9 33.66742516050847 37.0 33.0 39.0 23.0 39.0 10-11 33.55938813397623 37.0 33.0 39.0 24.0 39.0 12-13 33.54160327978754 37.0 33.0 39.0 24.0 39.0 14-15 33.61476033313565 38.0 32.0 40.0 17.5 41.0 16-17 33.72041874016966 37.5 32.5 40.0 19.5 41.0 18-19 33.98955083412835 38.0 33.0 40.0 20.5 41.0 20-21 34.24598084212155 38.0 33.5 40.0 23.5 41.0 22-23 34.18643155506278 38.0 33.5 40.0 22.5 41.0 24-25 33.815943841373794 38.0 33.0 40.0 19.5 41.0 26-27 33.38907833329036 37.5 32.5 40.0 17.0 41.0 28-29 33.42831910888792 37.0 33.0 40.0 18.0 41.0 30-31 33.12145140912256 37.0 33.0 40.0 16.5 41.0 32-33 32.42802258721605 35.5 31.5 40.0 12.5 41.0 34-35 31.959357192584378 35.0 31.0 39.0 9.0 41.0 36-37 31.45302387644071 35.0 30.5 39.0 7.0 41.0 38-39 30.988793414640433 35.0 30.0 39.0 2.0 40.0 40-41 30.804982208699688 35.0 30.0 39.0 2.0 40.0 42-43 30.092679137766545 35.0 29.0 39.0 2.0 40.0 44-45 29.399798236340665 35.0 26.0 39.0 2.0 40.0 46-47 28.61336474744089 35.0 23.5 38.0 2.0 40.0 48-49 27.850636876982183 35.0 22.5 38.0 2.0 40.0 50-51 26.921872985586468 33.5 19.0 37.5 2.0 39.5 52-53 27.07726645695279 34.0 18.5 38.0 2.0 40.0 54-55 27.046570017791304 34.5 13.0 38.0 2.0 40.0 56-57 26.395569579454914 34.0 6.5 38.0 2.0 40.0 58-59 25.86373539953072 33.5 2.0 38.0 2.0 40.0 60-61 24.99969380914318 32.5 2.0 38.0 2.0 40.0 62-63 24.641724466905604 32.0 2.0 38.0 2.0 40.0 64-65 24.048461954980276 31.5 2.0 36.5 2.0 39.5 66-67 23.11840561586262 31.0 2.0 36.0 2.0 39.0 68-69 22.39709731067736 30.0 2.0 35.5 2.0 38.5 70-71 21.46070120929273 29.0 2.0 35.0 2.0 37.0 72-73 20.612314029342755 27.0 2.0 34.0 2.0 36.5 74-75 20.267265941262927 27.0 2.0 34.0 2.0 36.0 76-77 19.776058453446097 26.5 2.0 34.0 2.0 35.5 78-79 18.971272851507102 26.0 2.0 34.0 2.0 35.0 80-81 18.066646468813655 23.5 2.0 33.0 2.0 35.0 82-83 17.23985057886187 20.0 2.0 32.5 2.0 35.0 84-85 16.52393123275662 16.0 2.0 32.0 2.0 34.0 86-87 15.962373978289456 4.5 2.0 32.0 2.0 34.0 88-89 15.016237784596344 2.0 2.0 31.0 2.0 34.0 90-91 13.71269950751618 2.0 2.0 30.0 2.0 34.0 92-93 12.531089652682876 2.0 2.0 29.0 2.0 34.0 94-95 12.002620349122038 2.0 2.0 28.5 2.0 34.0 96-97 11.544400897300363 2.0 2.0 28.0 2.0 34.0 98-99 11.023741072119229 2.0 2.0 27.0 2.0 34.0 100-101 9.828436428332001 2.0 2.0 22.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 2.432140654612187 1101 2 1.1335936900625256 1101 3 2.1261138835629083 1101 4 1.3779987018275364 1101 5 0.8343440623172427 1101 6 1.1070512859175707 1101 7 -0.9521859963772883 1101 8 -0.25067381861778415 1101 9 -0.4004757019752887 1101 10-11 1.1751292583923316 1101 12-13 1.2439644695791827 1101 14-15 1.5401846707963713 1101 16-17 -0.10680064234237818 1101 18-19 0.9535501025015662 1101 20-21 1.4226434757539508 1101 22-23 1.0105326663315566 1101 24-25 1.5694922915040408 1101 26-27 0.9960663830237664 1101 28-29 1.0835062690907051 1101 30-31 1.3800425560998946 1101 32-33 1.2619200552808252 1101 34-35 2.554485739093799 1101 36-37 0.4493817327105596 1101 38-39 0.1042045525073938 1101 40-41 -0.03229418179965293 1101 42-43 0.34688119118308336 1101 44-45 0.7949226941318415 1101 46-47 0.8641336598375666 1101 48-49 0.7287610061830563 1101 50-51 1.2067605614570986 1101 52-53 0.4914726251009114 1101 54-55 1.9213198870660797 1101 56-57 1.4439206100616708 1101 58-59 1.6225925551737959 1101 60-61 0.9317104533349472 1101 62-63 0.48480506742245666 1101 64-65 -0.3214304246990025 1101 66-67 0.5164863493423972 1101 68-69 0.35818407608590164 1101 70-71 0.0568910249659389 1101 72-73 -0.48991635012492196 1101 74-75 -0.12569357688798988 1101 76-77 -0.6987545337717727 1101 78-79 -1.0784007496981154 1101 80-81 -0.47932203486883296 1101 82-83 -1.9256072631760794 1101 84-85 -3.0904621392233533 1101 86-87 -2.6947543154222444 1101 88-89 -3.430589199171914 1101 90-91 -3.796315949725349 1101 92-93 -5.466566057281765 1101 94-95 -3.590893068053825 1101 96-97 0.10976621762985239 1101 98-99 1.5262768559987148 1101 100-101 1.8751949028886052 1102 1 2.2148645549831443 1102 2 1.602945394708346 1102 3 1.2737909724164673 1102 4 1.5998676295788172 1102 5 1.0555064011266353 1102 6 1.6914003408548623 1102 7 1.2230480612097097 1102 8 1.3887891737863853 1102 9 1.5597786700437908 1102 10-11 2.1031455099379883 1102 12-13 1.6719807211248465 1102 14-15 2.4758850770850174 1102 16-17 2.1519875576222915 1102 18-19 2.410006558958017 1102 20-21 1.7012161660913492 1102 22-23 1.8110979374850658 1102 24-25 2.3050513800043078 1102 26-27 2.513112841246695 1102 28-29 2.61247641570791 1102 30-31 2.87544972797766 1102 32-33 2.6409166989833537 1102 34-35 2.3697127307913775 1102 36-37 2.8297915542968504 1102 38-39 2.7775838141219538 1102 40-41 2.7893804339926085 1102 42-43 3.1460332844528303 1102 44-45 2.8089661493517646 1102 46-47 2.655424951128861 1102 48-49 2.522172064299994 1102 50-51 2.6323920753239065 1102 52-53 3.4234634394446672 1102 54-55 3.9280324820139647 1102 56-57 4.301896232743179 1102 58-59 3.283783157540867 1102 60-61 3.4195217940874656 1102 62-63 3.3749304869596415 1102 64-65 3.7915354823845533 1102 66-67 3.2721832226863263 1102 68-69 2.9384702446073625 1102 70-71 3.1063522508977535 1102 72-73 3.1525496452822495 1102 74-75 3.232900311117664 1102 76-77 2.980984028319728 1102 78-79 3.4292834050448633 1102 80-81 3.4814622788566574 1102 82-83 3.230107274891399 1102 84-85 2.7325373308349405 1102 86-87 2.4384377001227104 1102 88-89 1.4203205340907612 1102 90-91 -2.7346660645460528 1102 92-93 -6.054183086075264 1102 94-95 -4.945600716879122 1102 96-97 -2.3745121784132497 1102 98-99 -1.0028699378539603 1102 100-101 -0.14750426686938845 1103 1 2.5717273000811858 1103 2 2.1192852639893935 1103 3 1.880326920128887 1103 4 1.6495408321931961 1103 5 1.913676335767164 1103 6 1.441073543469237 1103 7 1.909976165784876 1103 8 1.6168937489497779 1103 9 2.1186021994555517 1103 10-11 1.8930147909837416 1103 12-13 1.7353794139353056 1103 14-15 1.6902641620523369 1103 16-17 1.820941805988305 1103 18-19 2.0475882583044296 1103 20-21 1.7910854471370996 1103 22-23 2.175477022452384 1103 24-25 1.612894517259214 1103 26-27 2.0382762399395062 1103 28-29 2.202345696753653 1103 30-31 1.642443192029944 1103 32-33 1.4533349996369438 1103 34-35 1.5121963909220995 1103 36-37 0.9958046261922711 1103 38-39 1.3596099579127952 1103 40-41 1.616831414384766 1103 42-43 0.9950528922959627 1103 44-45 0.8275936003321611 1103 46-47 -0.3517645913547973 1103 48-49 -0.14580179190915388 1103 50-51 -0.21009158480681478 1103 52-53 -0.22490257362722588 1103 54-55 -0.5938629428226356 1103 56-57 -0.6814371005901556 1103 58-59 -0.6449750123937719 1103 60-61 -0.45008604904979066 1103 62-63 -0.9685335653279381 1103 64-65 -1.267614844412833 1103 66-67 -1.8503657969215155 1103 68-69 -2.179176814216163 1103 70-71 -1.4737131085793713 1103 72-73 -1.3716333612537 1103 74-75 -0.9997794274444303 1103 76-77 -0.5781662984776545 1103 78-79 -0.9285597322100365 1103 80-81 -0.6381455642805953 1103 82-83 -0.35486004537004234 1103 84-85 -0.11550188485133361 1103 86-87 -0.11319628680539218 1103 88-89 -1.0368690083929 1103 90-91 -4.127476522062391 1103 92-93 -5.656470667774611 1103 94-95 -4.797888298578467 1103 96-97 -2.0415056424655376 1103 98-99 -0.6185562123637656 1103 100-101 0.0776591318234221 1104 1 1.283492005963538 1104 2 0.5166860847828261 1104 3 0.4985211745748508 1104 4 0.8055599840399807 1104 5 0.31470232755922467 1104 6 0.4672021344405124 1104 7 -0.09234941561047805 1104 8 0.3459635163916417 1104 9 0.48214529111081816 1104 10-11 0.3997521370849739 1104 12-13 0.1837036273415933 1104 14-15 0.41157059160089915 1104 16-17 0.15514153239049477 1104 18-19 0.28565255379005805 1104 20-21 0.345805009380598 1104 22-23 0.41538810316374253 1104 24-25 0.11364691124006043 1104 26-27 0.3298973069709632 1104 28-29 0.23278345325161354 1104 30-31 -0.6314282169987848 1104 32-33 -0.04543381021257886 1104 34-35 -0.28445203589048873 1104 36-37 -0.1292637732605293 1104 38-39 -0.2985295769709211 1104 40-41 -0.39079512460292065 1104 42-43 -0.5699949873209995 1104 44-45 -0.12941734358028967 1104 46-47 -0.39851561734659313 1104 48-49 -0.46262805729902823 1104 50-51 -0.07938023049764809 1104 52-53 -0.43553868853830835 1104 54-55 -0.722693312179679 1104 56-57 -0.8653974289075279 1104 58-59 -0.9100912914635408 1104 60-61 -0.3610299614984882 1104 62-63 -0.7238687226466816 1104 64-65 0.0806751692670602 1104 66-67 -0.5690730472635117 1104 68-69 -0.6437800973898966 1104 70-71 -0.04587453128798913 1104 72-73 -0.4665034022933696 1104 74-75 -0.7602787434499021 1104 76-77 -0.7685219756322361 1104 78-79 -0.37640514944670045 1104 80-81 0.11079424420093531 1104 82-83 -0.1973703052879614 1104 84-85 -0.3212132391604996 1104 86-87 0.14080508118366275 1104 88-89 0.14271375494499416 1104 90-91 -2.4609922539365385 1104 92-93 -4.847065743014009 1104 94-95 -3.2118075872241594 1104 96-97 -1.6638038640797639 1104 98-99 -0.9731731754280606 1104 100-101 -1.48393457542692 1105 1 2.1948844110268304 1105 2 1.5299330674145608 1105 3 0.9690834353932196 1105 4 0.6304790302572272 1105 5 0.9272280855810067 1105 6 0.5299045188973821 1105 7 1.1087103430000624 1105 8 1.5257545084434483 1105 9 0.8579916261122946 1105 10-11 1.0736923784744334 1105 12-13 0.6172111339650925 1105 14-15 1.1751487487984278 1105 16-17 0.7108524537917305 1105 18-19 0.9894721004488787 1105 20-21 1.1615247621035891 1105 22-23 0.9154249003377188 1105 24-25 1.117473817333675 1105 26-27 0.7980677514808292 1105 28-29 0.7345497176025049 1105 30-31 1.3300083446732813 1105 32-33 0.8914958335907812 1105 34-35 0.8168873812422746 1105 36-37 0.2620741719852795 1105 38-39 0.41150869209001684 1105 40-41 0.23310096017776516 1105 42-43 0.736378283211824 1105 44-45 1.0214878668995446 1105 46-47 0.7637231227181722 1105 48-49 0.13562039721966102 1105 50-51 -0.044864481307186566 1105 52-53 -0.14024136737257464 1105 54-55 0.5324214950031312 1105 56-57 0.09924048690053766 1105 58-59 0.001227519251795428 1105 60-61 -0.4558194816633403 1105 62-63 -0.47799000613211007 1105 64-65 -0.5235344274359157 1105 66-67 0.06549421797052091 1105 68-69 0.396586402462912 1105 70-71 0.522936184049076 1105 72-73 0.49169500806871724 1105 74-75 0.7048743327938425 1105 76-77 1.000612554835822 1105 78-79 0.8415445120193006 1105 80-81 0.8846020157640773 1105 82-83 0.5405234244735944 1105 84-85 0.8863596192439758 1105 86-87 1.177123891898999 1105 88-89 0.3407929424633931 1105 90-91 -1.863626931593295 1105 92-93 -4.3419509358312 1105 94-95 -3.3601369955256004 1105 96-97 -1.4262413088840038 1105 98-99 -0.6029568080450751 1105 100-101 0.5685749918383127 1106 1 1.5899436188667622 1106 2 0.7151106909343632 1106 3 -0.07109622977623786 1106 4 0.4639620845651109 1106 5 0.5502987266590011 1106 6 0.9171646250631653 1106 7 0.3906213270751948 1106 8 -0.6379449607276371 1106 9 -1.3715306278689283 1106 10-11 -1.3727347346329566 1106 12-13 -0.4942221040912571 1106 14-15 0.16616549032557515 1106 16-17 0.8637311798023433 1106 18-19 0.737246702327198 1106 20-21 0.3437419936266579 1106 22-23 0.2562170604030456 1106 24-25 0.3195358834831197 1106 26-27 -0.21457005987073785 1106 28-29 0.08612178783525337 1106 30-31 0.3653084671722624 1106 32-33 0.7947012235458644 1106 34-35 1.0810768463300633 1106 36-37 1.041535176476902 1106 38-39 0.9321906030740905 1106 40-41 1.2399813195081073 1106 42-43 1.4622255678177858 1106 44-45 0.944007262571251 1106 46-47 1.516356850770439 1106 48-49 1.1426232555291769 1106 50-51 0.663342950297551 1106 52-53 0.9620044472456399 1106 54-55 0.9751123892456768 1106 56-57 1.5689424060512103 1106 58-59 0.8679282134126787 1106 60-61 1.2555116739103624 1106 62-63 1.364578389131264 1106 64-65 1.1740360094771134 1106 66-67 0.7021009962473634 1106 68-69 0.714940832842661 1106 70-71 0.5704045384794547 1106 72-73 0.6663173028829235 1106 74-75 0.6666342347946603 1106 76-77 0.6560841759056437 1106 78-79 0.7857666435015354 1106 80-81 1.15616183609891 1106 82-83 0.6263543758823324 1106 84-85 0.8900958381088167 1106 86-87 0.5841471667050424 1106 88-89 -0.26049329681794653 1106 90-91 -2.6517649471098306 1106 92-93 -6.428292299653169 1106 94-95 -5.351398735011106 1106 96-97 -3.8220559270955192 1106 98-99 -2.9688408423447896 1106 100-101 -2.700044852996312 1107 1 2.064742005963538 1107 2 1.702005852224687 1107 3 1.051282802481829 1107 4 1.3546878910167237 1107 5 0.720661629884809 1107 6 0.9068823669986514 1107 7 1.0099761657848703 1107 8 1.3106437489497793 1107 9 2.045808081808495 1107 10-11 1.2005515556896285 1107 12-13 1.150636766876481 1107 14-15 1.0788671032288093 1107 16-17 1.6746182765765383 1107 18-19 1.4804926700691325 1107 20-21 1.5625928000782707 1107 22-23 1.0359549636288534 1107 24-25 0.5588503996121545 1107 26-27 1.0770630046453817 1107 28-29 1.3012427555771922 1107 30-31 1.5926270155593514 1107 32-33 2.5310923525781206 1107 34-35 2.51918168503974 1107 36-37 1.6117972732511028 1107 38-39 1.528359957912798 1107 40-41 1.7359490614435877 1107 42-43 1.7946852452371367 1107 44-45 1.8246524238615685 1107 46-47 2.1557721733510853 1107 48-49 1.938389384561436 1107 50-51 1.5467098857814214 1107 52-53 2.291641544019832 1107 54-55 2.0204752924714846 1107 56-57 2.0385996641157256 1107 58-59 2.5362749876062303 1107 60-61 2.374362480361974 1107 62-63 1.5163929052602967 1107 64-65 1.757201332057754 1107 66-67 1.9626856736667229 1107 68-69 1.1805658328426567 1107 70-71 1.2016545384794561 1107 72-73 1.1570431093345377 1107 74-75 1.472279396084982 1107 76-77 1.8918704662282302 1107 78-79 2.359859385437023 1107 80-81 2.0956779651311663 1107 82-83 2.061132601688783 1107 84-85 2.537071644560431 1107 86-87 2.590296360253431 1107 88-89 2.241623638665926 1107 90-91 -1.387954463238863 1107 92-93 -2.9757721383628457 1107 94-95 -1.341822122107878 1107 96-97 -0.1779027012890655 1107 98-99 -0.031240035893175033 1107 100-101 0.7399753082940101 1108 1 1.8926874082623897 1108 2 1.111847806247674 1108 3 0.016297170297921326 1108 4 0.10167064963741268 1108 5 0.0858196758618206 1108 6 0.5042243210216384 1108 7 0.8249186945205054 1108 8 0.7536753581451805 1108 9 1.1361816450268876 1108 10-11 0.9753001189080095 1108 12-13 1.0843652151523457 1108 14-15 0.43813434460811607 1108 16-17 1.3178654030133217 1108 18-19 0.9205429574254538 1108 20-21 1.333174696629996 1108 22-23 1.1670253659277066 1108 24-25 1.1344969513362955 1108 26-27 0.9969983494729746 1108 28-29 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2311 66-67 0.5555165560196613 2311 68-69 0.3149408328426624 2311 70-71 0.2674633620088649 2311 72-73 0.5636607563933609 2311 74-75 0.9590441019673328 2311 76-77 0.29242193681646356 2311 78-79 0.28614615014290123 2311 80-81 0.29420737689587284 2311 82-83 0.19219877815936925 2311 84-85 1.0462628210310179 2311 86-87 1.3868037131946078 2311 88-89 0.7866604033718065 2311 90-91 2.1342881838199617 2311 92-93 3.111176391048918 2311 94-95 2.834464642598004 2311 96-97 1.8173178869462276 2311 98-99 1.310855552342117 2311 100-101 1.577659131823422 2312 1 0.6893743589047148 2312 2 0.36046173457762976 2312 3 -0.5432024916358209 2312 4 -0.5092826972185733 2312 5 0.11367633576715974 2312 6 -0.6118676330013457 2312 7 0.16879969519663973 2312 8 -0.29487095693257004 2312 9 -0.004927212309155493 2312 10-11 0.26360302627785615 2312 12-13 0.08537941393530701 2312 14-15 0.7579112208758616 2312 16-17 0.17976533540007011 2312 18-19 -0.6906470358132282 2312 20-21 -0.520679258745254 2312 22-23 0.03724172833474171 2312 24-25 0.30701216431803147 2312 26-27 0.42062918111597725 2312 28-29 0.4464633438124821 2312 30-31 0.13361966261818026 2312 32-33 -0.5937238238924643 2312 34-35 -0.24662713848967144 2312 36-37 0.6046281556040363 2312 38-39 1.162551134383392 2312 40-41 0.852125532031824 2312 42-43 0.6921117158253729 2312 44-45 1.2422994826851017 2312 46-47 1.5658824674687288 2312 48-49 1.6130217375026135 2312 50-51 1.5369672387225997 2312 52-53 1.6456856616668887 2312 54-55 1.1090782336479528 2312 56-57 1.2715040758804328 2312 58-59 1.09914263466505 2312 60-61 1.776384539185507 2312 62-63 1.7020546699661772 2312 64-65 1.7117969202930503 2312 66-67 1.8231636148431924 2312 68-69 1.9296467151955987 2312 70-71 1.6733457149500417 2312 72-73 1.0489548740404189 2312 74-75 0.9443382196143943 2312 76-77 1.6247748779929339 2312 78-79 0.6126167383781969 2312 80-81 0.6206779651311685 2312 82-83 0.8569046605123134 2312 84-85 1.1021451739721968 2312 86-87 0.9544507720181361 2312 88-89 0.7190133445482783 2312 90-91 1.1460528897023146 2312 92-93 1.7464705086959764 2312 94-95 1.4756411131862386 2312 96-97 1.4879061222403447 2312 98-99 2.0079143758715308 2312 100-101 1.5453061906469525 2313 1 -6.844414970780647 2313 2 -2.4193604268450812 2313 3 -1.536362546355381 2313 4 -1.7874632717739765 2313 5 -0.37134418406868264 2313 6 0.2346439949056247 2313 7 0.3611389564825487 2313 8 0.6773588652288538 2313 9 0.6914476166922157 2313 10-11 0.32998469522451046 2313 12-13 0.25637804594624924 2313 14-15 -0.007034059561888739 2313 16-17 -0.21695149086531984 2313 18-19 0.00948976309238958 2313 20-21 -0.11931126968917027 2313 22-23 -0.7735653852083502 2313 24-25 0.29678644612378235 2313 26-27 0.018850795343062998 2313 28-29 -0.5898909653530424 2313 30-31 -0.27387007746390424 2313 32-33 -0.8826431125381546 2313 34-35 -0.32224273356490585 2313 36-37 -0.6583335407023903 2313 38-39 -0.5427156234825503 2313 40-41 -0.37044628739362295 2313 42-43 -0.13104149894890682 2313 44-45 -0.6003475761384287 2313 46-47 -2.1601435243233347 2313 48-49 -1.549837359624611 2313 50-51 -1.5939151142185786 2313 52-53 -0.9907712466778449 2313 54-55 -0.7401351726447949 2313 56-57 -0.7462113823959022 2313 58-59 -0.6746261751844713 2313 60-61 -0.3232392638240711 2313 62-63 -0.822705931949006 2313 64-65 -1.1111852958492214 2313 66-67 -0.7754683961007238 2313 68-69 -0.8559893997154795 2313 70-71 -0.9063396475670551 2313 72-73 -1.1845266581073233 2313 74-75 -0.873650836473157 2313 76-77 -1.1173591849345605 2313 78-79 -0.8211725913071639 2313 80-81 -0.7932755232409257 2313 82-83 -1.0491144913344712 2313 84-85 -1.3328411461372447 2313 86-87 -0.37082282579308057 2313 88-89 -0.3078676404038436 2313 90-91 0.14365890885416022 2313 92-93 1.2227016988464552 2313 94-95 1.5643552034735144 2313 96-97 0.9524752056876782 2313 98-99 0.552989615269615 2313 100-101 0.6032747268986629 2314 1 1.3284021856042578 2314 2 0.708929504919297 2314 3 -0.33221270650020784 2314 4 1.070181902992772 2314 5 1.1623906718009778 2314 6 -1.7519873934803911 2314 7 -0.9397244330175205 2314 8 -0.8627469696130952 2314 9 -2.556811678670549 2314 10-11 -0.9078129652684623 2314 12-13 0.19994440160701998 2314 14-15 0.11932369005515397 2314 16-17 -0.2777020827048915 2314 18-19 -1.4998404137632022 2314 20-21 -1.193151960400769 2314 22-23 -3.237080216011865 2314 24-25 -2.0798846303279674 2314 26-27 -0.5290185821809601 2314 28-29 -0.7623425737641298 2314 30-31 -0.8311566670753763 2314 32-33 -1.46174073125421 2314 34-35 -0.26304136885246976 2314 36-37 -0.534029822557283 2314 38-39 -3.2179349522668375 2314 40-41 -2.465697645143237 2314 42-43 -1.6439749344035768 2314 44-45 -1.9004972767372301 2314 46-47 -1.3528917488046055 2314 48-49 -2.9886689986720967 2314 50-51 -2.409034874697621 2314 52-53 -1.8710455817286729 2314 54-55 -1.82596931830696 2314 56-57 -0.7116061742076276 2314 58-59 -0.6365917788608364 2314 60-61 -0.9664122202368262 2314 62-63 -1.0426265558175487 2314 64-65 -0.3191646859063155 2314 66-67 0.2749424101936633 2314 68-69 0.1628450244594255 2314 70-71 0.8009434606351427 2314 72-73 -0.03345090264151196 2314 74-75 0.03590215057600332 2314 76-77 0.9329819931743337 2314 78-79 0.9130218105867236 2314 80-81 1.5985222765084117 2314 82-83 1.777580954982195 2314 84-85 0.09451924935084932 2314 86-87 1.7773285458821757 2314 88-89 2.838386363216822 2314 90-91 3.4895717343659243 2314 92-93 3.8236103466670937 2314 94-95 4.18439419525739 2314 96-97 3.328571849609135 2314 98-99 3.284085562909219 2314 100-101 3.124911685539521 2315 1 -2.299841327369794 2315 2 -1.922192865724032 2315 3 -1.6152236077745812 2315 4 -2.26614544231661 2315 5 -2.8993704213972435 2315 6 -1.8695599406936552 2315 7 -4.42463921883051 2315 8 -2.8561831741271426 2315 9 -1.680794482294072 2315 10-11 -2.8961631879001217 2315 12-13 -2.0901484895337745 2315 14-15 -0.9280239224122155 2315 16-17 -2.7359586465003787 2315 18-19 -0.9340105350590733 2315 20-21 -0.19533989222941983 2315 22-23 -0.36204183124294076 2315 24-25 -2.0243226773109235 2315 26-27 -3.315004303046919 2315 28-29 -2.6896226290381975 2315 30-31 -2.442549266491927 2315 32-33 -2.92155187819111 2315 34-35 -2.582901648293589 2315 36-37 -2.6286674703386446 2315 38-39 -1.1955182472154036 2315 40-41 -0.9653329898384619 2315 42-43 -1.7881673188654226 2315 44-45 -5.395219371010224 2315 46-47 -1.0261989804950709 2315 48-49 -0.3223477949257436 2315 50-51 -1.7894279347313997 2315 52-53 -0.9261469175186292 2315 54-55 0.18569965144584089 2315 56-57 0.9018208179618803 2315 58-59 -0.42894937136813027 2315 60-61 -1.8169035452790503 2315 62-63 -2.8279981203807303 2315 64-65 -2.8659557192242957 2315 66-67 -2.191240608384561 2315 68-69 -1.115828397926574 2315 70-71 -2.016133923059005 2315 72-73 -0.9022517624603346 2315 74-75 0.5812537550593397 2315 76-77 -0.727600687617926 2315 78-79 0.5567343854370215 2315 80-81 0.29952411897732034 2315 82-83 0.016180678611860344 2315 84-85 -0.5470629708241841 2315 86-87 -0.19536069102862008 2315 88-89 0.26942812584541187 2315 90-91 0.9145295111201115 2315 92-93 2.244019528303822 2315 94-95 1.3710785189177628 2315 96-97 0.649180632044267 2315 98-99 0.012590092311951295 2315 100-101 -0.09760481991111725 2316 1 -6.654416490768487 2316 2 -6.011433690258972 2316 3 -2.778496609282879 2316 4 -3.4524199521205325 2316 5 -1.5065851021413366 2316 6 -1.9752663258118055 2316 7 -2.3128996512085926 2316 8 -2.049119322945643 2316 9 -7.472901068518304 2316 10-11 -6.360906777643713 2316 12-13 -5.0633133965222115 2316 14-15 -3.6482979294509335 2316 16-17 -4.099646429305814 2316 18-19 -1.8612352711073399 2316 20-21 -0.9196988665883978 2316 22-23 -1.633346506959377 2316 24-25 -1.5184780317603952 2316 26-27 -2.07512245287095 2316 28-29 -2.9430791398476472 2316 30-31 -6.330432624963521 2316 32-33 -3.5355538892519434 2316 34-35 -4.198914720189016 2316 36-37 -2.5492934130234097 2316 38-39 -4.389082852544718 2316 40-41 -1.0995084548962808 2316 42-43 -3.2984111599916197 2316 44-45 -1.5929946349619577 2316 46-47 -3.089673088086826 2316 48-49 -4.000703752693468 2316 50-51 -3.553882434480016 2316 52-53 -5.6713078023853996 2316 54-55 -3.866085165044854 2316 56-57 -3.972613571178389 2316 58-59 -3.0560861235048833 2316 60-61 -3.420347486958285 2316 62-63 -4.562978009772387 2316 64-65 -3.770556020883422 2316 66-67 -5.178797169470538 2316 68-69 -4.019046095261917 2316 70-71 -3.6256738928930936 2316 72-73 -3.8441823808615396 2316 74-75 -3.8978186431307016 2316 76-77 -3.7608460370397445 2316 78-79 -5.701435549203502 2316 80-81 -5.5740932766989 2316 82-83 -3.5444025290301706 2316 84-85 -2.87857378027617 2316 86-87 -2.927575371772713 2316 88-89 -1.6940585508765604 2316 90-91 -1.5941431887290598 2316 92-93 -1.6358824324804946 2316 94-95 -1.6841628083823892 2316 96-97 -3.1588259039034465 2316 98-99 -4.447314382298405 2316 100-101 -3.5952166851700422 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5517.0 3 927.0 4 678.0 5 572.0 6 577.0 7 593.0 8 820.0 9 1015.0 10 1310.0 11 1853.0 12 2559.0 13 3158.0 14 3429.0 15 4095.0 16 4438.0 17 4579.0 18 3870.0 19 3569.0 20 3761.0 21 3638.0 22 3919.0 23 3979.0 24 4179.0 25 4543.0 26 4733.0 27 5237.0 28 5710.0 29 6208.0 30 6925.0 31 7400.0 32 8253.0 33 8775.0 34 9543.0 35 9485.0 36 8541.0 37 5546.0 38 1166.0 39 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.74207624875738 19.704481196018435 13.757956027215101 12.79548652800909 2 14.784791826671745 29.402425408053283 31.167590662372614 24.645192102902357 3 31.161571826913146 28.397707322110914 29.79254879686048 10.64817205411546 4 11.331375270309525 17.96404479876061 40.2614336894426 30.443146241487266 5 8.502494658912678 45.36729253667762 31.43722044006687 14.692992364342835 6 23.015150634880673 39.259962171827695 13.937034813538096 23.787852379753534 7 19.586610359924066 40.398150659279636 21.50116875234073 18.514070228455566 8 24.718658600362854 23.463517622979925 19.140254256438734 32.67756952021848 9 21.457398379233524 24.526523100765182 26.164078390856556 27.852000129144738 10-11 25.603220620234126 26.122665082613945 22.78324089438716 25.490873402764773 12-13 34.71686906445095 26.674865875152527 22.63207504535389 15.976190015042643 14-15 25.343960435672457 28.591489279281024 32.95725270681206 13.107297578234455 16-17 15.78119956097876 32.220285363806575 31.726709277551812 20.271805797662857 18-19 14.86044521930037 36.077614735176844 32.082909583999225 16.97903046152356 20-21 20.648306589610304 27.62018532270042 29.41723436541504 22.31427372227424 22-23 16.666989626529215 29.157139350721494 32.45068402898888 21.725186993760413 24-25 23.292405991478585 29.42181650208513 29.59818847250891 17.687589033927377 26-27 20.38877922547838 24.356692178216864 38.89310671236992 16.36142188393483 28-29 15.009465719879303 37.023887212555486 32.259044123823244 15.707602943741964 30-31 17.586900835656397 29.352955807440473 31.3069123352187 21.753231021684428 32-33 17.945156361545493 30.885321945531075 34.08135774991843 17.088163943004997 34-35 21.33967706405424 28.428247043737503 32.30457659473445 17.927499297473812 36-37 18.297343131712832 26.891383348138575 38.0631510942421 16.748122425906484 38-39 17.534277547197235 30.6577736147672 35.251368679446394 16.55658015858917 40-41 16.52152566024084 32.289782417696 33.242674040002065 17.94601788206109 42-43 15.110300054907789 32.35909692839379 29.286198766189724 23.244404250508705 44-45 18.228677175882417 26.83402608895047 31.77632875048848 23.160967984678635 46-47 21.404115119997417 31.130850479666655 31.9729319422462 15.492102458089732 48-49 18.984842756931585 29.16452604131326 32.83767759112602 19.012953610629133 50-51 12.27458519799021 30.90779843934278 37.880026495629835 18.937589867037175 52-53 17.217768385406064 33.23736311318346 28.744211897075978 20.800656604334495 54-55 14.298177870121142 27.340026432031227 32.40638117057061 25.955414527277014 56-57 19.76003799075429 25.910598968176284 30.606786001570025 23.722577039499402 58-59 24.448801982213062 25.21377599312557 29.551320775440715 20.78610124922065 60-61 18.222453323170633 27.412806939918415 35.017068814613616 19.34767092229733 62-63 16.225904480176197 27.281182484910527 33.575970521842194 22.916942513071085 64-65 15.374504681106671 32.488075207735214 30.024317856669974 22.113102254488144 66-67 18.3424518612778 26.513923249339527 27.677585215711858 27.466039673670817 68-69 16.14888978603149 26.626402906742026 30.389987888574886 26.83471941865159 70-71 21.481103463422986 26.397658967658998 28.11758132083577 24.003656248082248 72-73 18.756199134786556 22.06197357835868 35.19913155573648 23.982695731118284 74-75 15.598577101150532 30.5848941387811 29.991373433405816 23.825155326662554 76-77 17.844078794302405 23.78079518134581 30.071995450197765 28.30313057415402 78-79 18.45018450184502 24.89320865187636 31.99087507512553 24.665731771153087 80-81 21.325599056375122 23.806169109211652 30.273554266509393 24.594677567903826 82-83 18.642265755779757 23.196592576314785 33.4731549046348 24.68798676327066 84-85 17.815221078819405 25.954346456438504 31.48511332611751 24.745319138624577 86-87 18.286622680892037 26.363677486477926 30.113669408776904 25.23603042385313 88-89 18.141076800294677 25.854063607665488 27.402089236843718 28.60277035519611 90-91 20.258581620893708 21.97749550039493 29.322210568568803 28.441712310142563 92-93 22.75699101791555 24.71612917864656 29.485612781723354 23.041267021714535 94-95 21.00342473506004 23.205409723142996 29.576904543999277 26.214260997797687 96-97 17.093832012514383 23.915980401029074 32.13953277914959 26.850654807306952 98-99 20.994267942181544 25.656212315680232 26.572113037602684 26.777406704535533 100-101 19.664885034614105 22.384218167473414 28.505789495807832 29.445107302104656 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1484.0 1 985.5 2 537.5 3 561.5 4 425.5 5 347.0 6 481.5 7 815.5 8 1161.0 9 1171.5 10 859.5 11 582.5 12 487.0 13 423.0 14 413.0 15 450.5 16 440.5 17 438.5 18 484.5 19 567.5 20 657.5 21 735.5 22 832.0 23 1000.0 24 1174.5 25 1324.0 26 1504.0 27 1745.0 28 1926.0 29 2035.0 30 2187.0 31 2342.0 32 2449.0 33 2466.0 34 2495.5 35 2463.0 36 2219.5 37 2064.5 38 1997.5 39 1783.5 40 1535.0 41 1380.5 42 1317.0 43 1266.0 44 1399.5 45 2177.5 46 5230.5 47 11951.5 48 21027.5 49 22039.0 50 14074.0 51 7877.0 52 4675.0 53 2885.5 54 1854.0 55 1216.0 56 908.0 57 710.0 58 548.5 59 475.5 60 460.5 61 387.5 62 290.5 63 251.0 64 244.0 65 218.5 66 184.0 67 157.0 68 122.0 69 91.5 70 67.0 71 47.0 72 45.5 73 44.5 74 38.0 75 33.5 76 23.0 77 14.0 78 14.5 79 17.5 80 19.0 81 16.0 82 8.5 83 5.0 84 4.0 85 1.5 86 1.0 87 0.5 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14052548797153394 2 0.12183173039733904 3 0.1315009153495088 4 0.13988087564138926 5 0.12956707835907486 6 0.14245932496196786 7 0.17146687981847716 8 0.1605084702060181 9 0.17211149214862181 10-11 0.164376144186886 12-13 0.1540623469045716 14-15 0.1579300208854395 16-17 0.15599618389500552 18-19 0.17049996132326017 20-21 0.17211149214862181 22-23 0.20240827166542041 24-25 0.22271356006497695 26-27 0.23560580666786993 28-29 0.2339942758425083 30-31 0.22142433540468764 32-33 0.2233581723951216 34-35 0.21497821210324108 36-37 0.2220689477348323 38-39 0.2123997627826625 40-41 0.21916819224918135 42-43 0.2107882319573009 44-45 0.2033751901606374 46-47 0.21723435525874737 48-49 0.24978727793105227 50-51 0.25107650259134157 52-53 0.2555887889023541 54-55 0.25494417657220947 56-57 0.23044890802671275 58-59 0.22915968336642342 60-61 0.2046644148209267 62-63 0.19692906685919087 64-65 0.19821829151948017 66-67 0.20563133331614364 68-69 0.20595363948121598 70-71 0.21175515045251786 72-73 0.2401180929788825 74-75 0.24334115462960573 76-77 0.2578449320578604 78-79 0.2520434210865586 80-81 0.2639687491942346 82-83 0.26493566768945154 84-85 0.24463037928989503 86-87 0.24817574710569063 88-89 0.24914266560090761 90-91 0.43446871051749475 92-93 0.5675811566923652 94-95 0.3361653301704355 96-97 0.2771833019621999 98-99 0.30651316298378156 100-101 0.3213392465771085 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 155132.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.115927081453215 #Duplication Level Percentage of deduplicated Percentage of total 1 87.05056654828344 33.18013046953563 2 5.929308303737527 4.520021658974293 3 2.354134956874683 2.6919010906840626 4 1.1178758667343143 1.7043550009024573 5 0.7373583629291391 1.4052548797153392 6 0.517503805175038 1.1835082381455793 7 0.3788263148993743 1.0107521336668126 8 0.2942668696093354 0.8973003635613541 9 0.1555893793336716 0.533739009359771 >10 1.219347201082361 8.938839182115874 >50 0.11669203450025369 2.9748859036175643 >100 0.076103500761035 5.561070572157904 >500 0.018603077963808556 4.758528221127814 >1k 0.030441400304414005 22.172085707655416 >5k 0.003382377811601556 8.467627568780136 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7968 5.136271046592579 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5168 3.3313565221875563 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3343 2.1549390196735683 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2867 1.8481035505247145 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2487 1.6031508650697472 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2382 1.5354665704045587 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2090 1.3472397700023206 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2084 1.3433720960214528 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2069 1.333702911069283 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2059 1.3272567877678365 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1888 1.217028079313101 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1808 1.165459092901529 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1764 1.1370961503751644 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1597 1.0294458912410078 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1594 1.0275120542505738 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1536 0.9901245391021839 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1293 0.8334837428770339 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1272 0.8199468839439962 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1186 0.7645102235515561 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1077 0.6942474795657891 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 978 0.6304308588814687 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 943 0.6078694273264059 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 781 0.5034422298429725 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 708 0.4563855297424129 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 695 0.4480055694505325 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 605 0.38999045973751384 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 591 0.38096588711548873 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 542 0.34937988293840083 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 535 0.3448675966273883 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 502 0.3235953897326148 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 502 0.3235953897326148 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 432 0.2784725266224892 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 417 0.2688033416703195 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 368 0.23721733749323157 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 346 0.22303586623004926 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 330 0.21272206894773485 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 295 0.19016063739267206 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 289 0.18629296341180415 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 283 0.18242528943093625 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 281 0.18113606477064692 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 263 0.1695330428280432 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 251 0.1617976948663074 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 248 0.15986385787587343 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 243 0.1566407962251502 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 216 0.1392362633112446 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 207 0.13343475233994276 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 197 0.12698862903849625 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 182 0.11731944408632647 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 178 0.11474099476574788 No Hit AAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 171 0.11022870845473533 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 171 0.11022870845473533 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 165 0.10636103447386742 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 161 0.10378258515328881 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 157 0.10120413583271021 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 156 0.10055952350256556 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.44612330144651E-4 0.0 16-17 0.0 0.0 0.0 6.44612330144651E-4 0.0 18-19 0.0 0.0 0.0 0.001289224660289302 0.0 20-21 0.0 0.0 0.0 0.0035453678157955806 0.0 22-23 0.0 0.0 0.0 0.01289224660289302 0.0 24-25 0.0 0.0 0.0 0.030296779516798596 0.0 26-27 0.0 0.0 0.0 0.08734497073460021 0.0 28-29 0.0 0.0 0.0 0.23463888817265297 0.0 30-31 0.0 0.0 0.0 0.5479204806229534 0.0 32-33 0.0 0.0 0.0 1.080370265322435 0.0 34-35 0.0 0.0 0.0 1.8207075264935666 0.0 36-37 0.0 0.0 0.0 2.7370239537941883 0.0 38-39 0.0 0.0 0.0 3.9395482556790347 0.0 40-41 0.0 0.0 0.0 5.157543253487352 0.0 42-43 0.0 0.0 0.0 6.213740556429363 0.0 44-45 0.0 0.0 0.0 7.161642987907072 0.0 46-47 0.0 0.0 0.0 8.30744140473919 0.0 48-49 0.0 0.0 0.0 9.250509243740815 0.0 50-51 0.0 0.0 0.0 10.185519428615631 0.0 52-53 0.0 0.0 0.0 11.00192094474383 0.0 54-55 0.0 0.0 0.0 11.813810174561018 0.0 56-57 0.0 0.0 0.0 12.951228631101255 0.0 58-59 0.0 0.0 0.0 13.7321764690715 0.0 60-61 0.0 0.0 0.0 14.635600649769229 0.0 62-63 0.0 0.0 0.0 15.449101410411778 0.0 64-65 0.0 0.0 0.0 16.155274218085243 0.0 66-67 0.0 0.0 0.0 16.994559471933577 0.0 68-69 0.0 0.0 0.0 18.001121625454452 0.0 70-71 0.0 0.0 0.0 18.967395508341284 0.0 72-73 0.0 0.0 0.0 19.70579893252198 0.0 74-75 0.0 0.0 0.0 20.67400665239925 0.0 76-77 0.0 0.0 0.0 21.341825026429106 0.0 78-79 0.0 0.0 0.0 22.58302606812263 0.0 80-81 0.0 0.0 0.0 23.653727148492898 0.0 82-83 0.0 0.0 0.0 24.35538766985535 0.0 84-85 0.0 0.0 0.0 25.201441353170203 0.0 86-87 0.0 0.0 0.0 25.884730423123536 0.0 88-89 0.0 0.0 0.0 26.656009076141608 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCCA 35 3.608875E-8 81.303505 1 ATGATAT 20 0.002083334 71.140564 7 GGGCTAT 25 0.0050436687 56.912457 1 GTACTAG 35 2.8015664E-4 54.20234 1 ACCCATG 60 1.4996458E-6 47.427048 3 AAGTAAG 80 4.2418833E-9 47.427044 1 TCCATGG 40 5.4145034E-4 47.427044 2 GTGCTTA 40 5.4145034E-4 47.427044 1 TATTCAG 45 9.672083E-4 42.157375 5 TACCCAT 70 4.331483E-6 40.651752 2 GTCGCTG 25 0.001605643 38.040092 94-95 ATATCAA 75 6.956494E-6 37.94164 1 ATGGGAG 70 2.0636969E-4 33.87646 5 CCTCGCT 30 0.0039511956 31.659 68-69 GTATTAA 60 0.003970231 31.618032 1 GAGTAAA 160 1.9099389E-9 29.641903 1 AGTAAAA 170 3.6488927E-9 27.898262 2 ATATCGG 35 0.008326021 27.18031 84-85 TGGGTTA 35 0.008385506 27.140682 54-55 ATGGGGT 70 0.008432492 27.10117 5 >>END_MODULE