FastQCFastQC Report
Fri 27 May 2016
SRR939793_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939793_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2311499
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35950.1555267815387331No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28980.12537318856724575No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46000.032.4858471
GTATCAA70200.029.9369871
TATCAAC83350.025.3846242
ATCAACG82550.025.3454863
TCAACGC83550.025.0989134
CAACGCA84450.024.5510645
AACGCAG88300.023.8567246
TACATGG61600.023.4117742
GTACATG65200.022.9922241
ACATGGG63150.021.8624483
CGCAGAG95900.021.6678498
ACGCAGA96100.021.5254217
GAGTACT51250.019.34716812-13
AGAGTAC78000.019.00585710-11
GCAGAGT101700.018.752779
GTACTTT55100.017.9974714-15
AGTACTT55600.016.98021912-13
CAGAGTA97750.016.74304210-11
GAGTACA49250.016.5677551
CCCTATA5550.016.24062
Produced by FastQC (version 0.11.5)