FastQCFastQC Report
Fri 27 May 2016
SRR939793_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939793_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2311499
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42180.18247898874280283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35260.15254170562046535No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50000.036.0406881
GTATCAA71450.027.3336621
ATCAACG83100.023.1540623
TCAACGC83450.023.1119634
CAACGCA84600.022.7977945
TATCAAC86400.022.3803252
AACGCAG86750.022.3250626
GTACATG68200.022.2726141
TACATGG67350.021.9181042
ACATGGG65000.021.330553
GTGTAGC16350.020.4852051
ACGCAGA96800.019.9098647
CGCAGAG96600.019.7559648
GAGTACT51650.019.47857912-13
AGAGTAC81600.018.42132610-11
GTACTTT54800.017.92935414-15
CATGGGG40700.017.2636134
GCAGAGT108050.017.1827169
AGTACTT56750.016.73165512-13
CAGAGTA103650.016.73011210-11
Produced by FastQC (version 0.11.5)