FastQCFastQC Report
Fri 27 May 2016
SRR939792_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939792_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2399284
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35900.14962797234508296No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28680.11953566147233925No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA71750.030.6190851
GGTATCA49000.029.2820191
ATCAACG84200.025.6463173
TATCAAC85450.025.4357932
TCAACGC84900.025.3240384
CAACGCA85650.025.1582645
AACGCAG88750.024.3326
TACATGG63600.023.7445142
GTACATG66400.023.5924681
ACATGGG66900.022.151793
ACGCAGA97800.021.8879977
CGCAGAG99300.021.3678478
CATGGGG41550.019.9281064
GAGTACT50900.019.2705712-13
AGAGTAC77000.018.23727210-11
GCAGAGT106000.018.0643379
GTACTTT55200.017.68770414-15
GTATAGG6350.017.0392841
CCTACAC10300.016.9000853
AGTACTT56700.016.67705212-13
Produced by FastQC (version 0.11.5)