FastQCFastQC Report
Fri 27 May 2016
SRR939792_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939792_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2399284
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41570.17326002257340106No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36930.1539209197410561No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24110.10048831234651671No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54250.032.8919451
GTATCAA77400.026.1198141
ATCAACG89450.022.1684043
TCAACGC89850.022.0697144
CAACGCA90300.021.959735
TATCAAC93000.021.4805932
AACGCAG92800.021.3681436
TACATGG67300.020.729052
GTACATG71700.020.716991
ACATGGG68050.019.9377613
ACGCAGA103300.019.1043177
GAGTACT53300.018.95792812-13
CGCAGAG103550.018.9207538
AGAGTAC82700.018.7577510-11
GTACTTT57700.017.55337314-15
GTGTAGC16850.017.461971
AGTACTT58450.017.36871712-13
GTAAGGT9550.016.8893934
TAAGGTG12050.016.5348685
GCAGAGT116500.016.4917839
Produced by FastQC (version 0.11.5)