##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939792_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2399284 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.379514888608433 31.0 31.0 34.0 30.0 34.0 2 31.628157400291087 31.0 31.0 34.0 30.0 34.0 3 31.675052640704475 31.0 31.0 34.0 30.0 34.0 4 35.481969620936916 37.0 35.0 37.0 33.0 37.0 5 35.21896240711813 37.0 35.0 37.0 32.0 37.0 6 35.232249704495175 37.0 35.0 37.0 32.0 37.0 7 35.13731054764671 37.0 35.0 37.0 32.0 37.0 8 35.094868302376874 37.0 35.0 37.0 32.0 37.0 9 36.69368111486593 39.0 35.0 39.0 32.0 39.0 10-11 36.55193653606659 38.0 35.0 39.0 32.0 39.0 12-13 36.478460657429466 38.0 35.0 39.0 32.0 39.0 14-15 37.537251530039796 39.0 36.0 41.0 32.0 41.0 16-17 37.44794905480135 39.0 36.0 41.0 32.0 41.0 18-19 37.40585837274787 39.0 36.0 41.0 32.0 41.0 20-21 37.30714496491453 39.0 36.0 40.5 31.0 41.0 22-23 37.18271659378381 39.0 36.0 40.0 31.0 41.0 24-25 37.04238868762514 39.0 36.0 40.0 31.0 41.0 26-27 36.756836622925846 39.0 35.5 40.0 30.0 41.0 28-29 36.540107798826654 38.0 35.0 40.0 30.0 41.0 30-31 36.29342274611926 38.0 35.0 40.0 30.0 41.0 32-33 36.31282811872209 38.0 35.0 40.0 30.0 41.0 34-35 36.19129144361401 38.0 35.0 40.0 30.0 41.0 36-37 36.26233138719718 38.0 35.0 40.0 30.0 41.0 38-39 36.18150539911073 38.0 35.0 40.0 30.0 41.0 40-41 36.11706242362305 38.0 35.0 40.0 29.5 41.0 42-43 35.92547714234747 38.0 35.0 40.0 29.0 41.0 44-45 35.719583008931 38.0 34.0 40.0 28.0 41.0 46-47 35.49479886499472 38.0 34.0 40.0 27.0 41.0 48-49 35.238362569833335 38.0 34.0 40.0 27.0 41.0 50-51 35.038584010896585 38.0 33.5 40.0 26.0 41.0 52-53 34.80303894828624 38.0 33.0 40.0 26.0 41.0 54-55 34.548757879434035 38.0 33.0 40.0 25.5 41.0 56-57 34.188470810458455 37.0 33.0 40.0 24.5 41.0 58-59 33.8326356946489 37.0 32.0 40.0 23.5 41.0 60-61 33.52935063127166 36.5 32.0 39.0 23.0 41.0 62-63 33.23525143334428 36.0 31.5 39.0 23.0 40.0 64-65 32.798444660990526 35.5 31.0 39.0 22.0 40.0 66-67 31.864713181099027 35.0 30.0 38.5 17.0 40.0 68-69 29.888457139713346 34.0 27.0 37.5 10.0 40.0 70-71 29.222626000090028 34.0 26.0 37.0 9.0 39.0 72-73 29.82008486698532 34.0 27.5 36.0 10.5 39.0 74-75 29.95291553646838 34.0 28.0 36.0 12.5 39.0 76-77 29.51075550039095 32.5 28.5 35.0 13.0 38.0 78-79 30.051804204921133 34.0 29.0 35.5 14.5 37.5 80-81 29.855218473511265 34.0 29.0 35.0 12.0 37.0 82-83 29.41114078199996 34.0 29.0 35.0 7.5 36.5 84-85 29.03858192694154 33.5 29.0 35.0 7.0 36.0 86-87 28.64466128228255 33.0 28.0 35.0 3.5 36.0 88-89 28.190804006528616 33.0 27.0 35.0 2.0 35.0 90-91 27.685523056045053 33.0 26.0 35.0 2.0 35.0 92-93 27.18920519621687 32.5 25.5 34.5 2.0 35.0 94-95 26.345753149689656 32.0 25.0 34.0 2.0 35.0 96-97 25.826515118677072 32.0 24.0 34.0 2.0 35.0 98-99 25.139073365220625 32.0 21.0 34.0 2.0 35.0 100-101 23.657120832715094 30.0 10.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.25676754579759375 1101 2 0.32497509731571483 1101 3 0.34630628739418867 1101 4 0.25092744965713365 1101 5 0.28700754052881905 1101 6 0.26859960239836767 1101 7 0.21574234334267572 1101 8 0.16031591382257915 1101 9 0.35927880710575977 1101 10-11 0.3547626169136109 1101 12-13 0.33737522253121455 1101 14-15 0.37279734631034245 1101 16-17 0.44028610470402896 1101 18-19 0.26293024908793683 1101 20-21 0.36083736503049835 1101 22-23 0.3850077136342591 1101 24-25 0.2707042751195772 1101 26-27 0.36889471640670735 1101 28-29 0.4047731526164924 1101 30-31 0.15091416473490682 1101 32-33 0.1599045285288483 1101 34-35 0.16318135072523887 1101 36-37 0.3418512101908888 1101 38-39 0.4000780483134676 1101 40-41 0.31950213690421236 1101 42-43 0.07035216610859862 1101 44-45 0.15762494491495715 1101 46-47 0.13265836466333525 1101 48-49 0.23939975954581172 1101 50-51 0.40663381155048484 1101 52-53 0.15320746089752646 1101 54-55 -0.07367420750088627 1101 56-57 -0.08461238455308262 1101 58-59 -0.038021265862596465 1101 60-61 -0.13390401199350066 1101 62-63 -0.18446440274521336 1101 64-65 -0.23253552838249192 1101 66-67 -0.260804126299508 1101 68-69 -9.485101853430855 1101 70-71 -6.962082517651954 1101 72-73 -1.7335391995754819 1101 74-75 -0.602567844717953 1101 76-77 -0.4096969753602444 1101 78-79 -0.42719023677946666 1101 80-81 -0.3965331404953325 1101 82-83 -0.34730888910703683 1101 84-85 -0.122500279398853 1101 86-87 -0.3737856628701124 1101 88-89 -0.13124873190844255 1101 90-91 -0.42168468887288313 1101 92-93 -0.6503164167294173 1101 94-95 -0.37447143280967055 1101 96-97 -0.7185646750427672 1101 98-99 -0.5905383892495486 1101 100-101 -0.6366883580784766 1102 1 0.14592963215068977 1102 2 0.0660612396378113 1102 3 0.1270446501550211 1102 4 0.1290531691311685 1102 5 0.20114344208045054 1102 6 0.16820934346878147 1102 7 0.18293919143840043 1102 8 0.1975224900179242 1102 9 0.2508565427284424 1102 10-11 0.323920163486882 1102 12-13 0.2603028941105734 1102 14-15 0.3627125497086894 1102 16-17 0.3612567258431909 1102 18-19 0.22885368894396407 1102 20-21 0.2595418253641739 1102 22-23 0.2043352587483156 1102 24-25 0.02042621360556751 1102 26-27 0.2645283399616787 1102 28-29 0.14376329480888472 1102 30-31 -0.053359713696274014 1102 32-33 -0.20209281245725208 1102 34-35 -0.19438346273845042 1102 36-37 -0.09991152145948234 1102 38-39 0.06322662882160301 1102 40-41 0.16005469296239028 1102 42-43 0.053527985295659164 1102 44-45 -0.02707064663457004 1102 46-47 -0.029665759402355718 1102 48-49 -0.07746163124816263 1102 50-51 0.12675200796081754 1102 52-53 -0.11463111750284583 1102 54-55 -0.41300394143735986 1102 56-57 -0.23580115832612591 1102 58-59 -0.4115082221023556 1102 60-61 -0.2511201541534618 1102 62-63 -0.289799860046962 1102 64-65 -0.19513584940978035 1102 66-67 0.06075009974909662 1102 68-69 -4.682698850690773 1102 70-71 0.14276118421422268 1102 72-73 0.3178175460509287 1102 74-75 0.06748955106978016 1102 76-77 -0.17272087405830305 1102 78-79 -0.25512820393091573 1102 80-81 -0.24353964210857626 1102 82-83 -0.29362631699749997 1102 84-85 -0.3593255951567542 1102 86-87 -0.3855440168756594 1102 88-89 -0.44087841558306806 1102 90-91 -0.42905923692158865 1102 92-93 -0.5642753479355385 1102 94-95 -0.34412705808327004 1102 96-97 -0.921530124075634 1102 98-99 -0.47780390151506325 1102 100-101 -0.4725233532954931 1103 1 0.206858385936588 1103 2 0.17963528464042966 1103 3 0.21676207558673255 1103 4 0.19138306146106032 1103 5 0.16379127044256592 1103 6 0.0774252069703607 1103 7 0.24865205000839552 1103 8 0.23391847498533735 1103 9 0.3019172280748421 1103 10-11 0.38519626833441833 1103 12-13 0.23718648670844544 1103 14-15 0.34976319747362083 1103 16-17 0.32035286779647265 1103 18-19 0.2573321152795316 1103 20-21 0.2312446463527138 1103 22-23 0.19201210571088012 1103 24-25 0.2604318428969066 1103 26-27 0.22575314690077164 1103 28-29 0.06599455561157441 1103 30-31 0.11247246999305105 1103 32-33 0.18167088416358723 1103 34-35 -0.10815064793420959 1103 36-37 0.13388682305314603 1103 38-39 0.2712482104146048 1103 40-41 0.25228484376396665 1103 42-43 0.028735476217434552 1103 44-45 0.19549337664373923 1103 46-47 0.09501923813977697 1103 48-49 0.20123808173726587 1103 50-51 0.01985105177414681 1103 52-53 0.18419894132721026 1103 54-55 0.1110289304526546 1103 56-57 0.24370195760556612 1103 58-59 0.19966901336059095 1103 60-61 0.09619935173747507 1103 62-63 0.019374695556159338 1103 64-65 -0.005693149252799401 1103 66-67 0.22399558442315382 1103 68-69 -4.088194386900593 1103 70-71 0.6063717006818798 1103 72-73 0.910124921215445 1103 74-75 0.6911699183215738 1103 76-77 0.3914645436985431 1103 78-79 0.157875379786951 1103 80-81 0.30877439306260257 1103 82-83 0.15426317446341997 1103 84-85 0.29420460480201527 1103 86-87 0.23624776277326376 1103 88-89 0.036787886368948364 1103 90-91 -0.040656607804674394 1103 92-93 0.03408906542887635 1103 94-95 -0.1565895955458103 1103 96-97 -0.581698130672212 1103 98-99 -0.13135471578015867 1103 100-101 -0.10398149832506576 1104 1 0.11740317731256766 1104 2 0.22315860325044667 1104 3 0.17395146299762132 1104 4 0.18981473941505556 1104 5 0.26079366395463666 1104 6 0.13268558592466206 1104 7 0.07314350552681503 1104 8 0.1055379618861707 1104 9 0.23109225933064437 1104 10-11 0.17159606106881142 1104 12-13 0.19499760947091005 1104 14-15 0.3034254737810471 1104 16-17 0.2858814381186505 1104 18-19 0.06688363602637537 1104 20-21 0.2325334487238493 1104 22-23 0.2477533076792824 1104 24-25 0.16676509181601062 1104 26-27 0.21306120186180522 1104 28-29 0.17029580867107796 1104 30-31 0.12602418005724303 1104 32-33 0.21320021175511528 1104 34-35 0.1297118363764227 1104 36-37 0.20521412814541407 1104 38-39 0.2588428537173897 1104 40-41 0.10851154021328568 1104 42-43 0.2287351074174211 1104 44-45 0.2113593067758046 1104 46-47 0.23496931491165185 1104 48-49 0.2955257998363763 1104 50-51 0.25076211688087113 1104 52-53 0.11788408938197392 1104 54-55 0.12610808722831024 1104 56-57 0.10015930772831183 1104 58-59 0.17804288805955792 1104 60-61 0.13679747159502398 1104 62-63 0.18440855139715495 1104 64-65 0.24049453430204437 1104 66-67 0.2795018300513341 1104 68-69 -4.187914990159069 1104 70-71 0.7391642912437995 1104 72-73 1.0903888345031874 1104 74-75 0.6964485221430827 1104 76-77 0.6134620198771366 1104 78-79 0.14717019113960106 1104 80-81 -0.04346188726966105 1104 82-83 0.07165120370680356 1104 84-85 0.08899828578263325 1104 86-87 0.012328838538309128 1104 88-89 -0.05871717909919738 1104 90-91 -0.09780693314775135 1104 92-93 -0.07610760947022754 1104 94-95 0.39926654909263704 1104 96-97 -0.06927064597433841 1104 98-99 -0.016159264067763246 1104 100-101 0.07417764256760151 1105 1 0.16585341616287153 1105 2 0.20934984789879607 1105 3 0.24811805189140657 1105 4 0.20809425082418187 1105 5 0.3233270779657431 1105 6 0.31434377380210776 1105 7 0.16587669592286147 1105 8 0.30787096290782756 1105 9 0.3089165428878857 1105 10-11 0.3153126200174583 1105 12-13 0.252150015681444 1105 14-15 0.29470213777563004 1105 16-17 0.32354679305038303 1105 18-19 0.2816464946649475 1105 20-21 0.31983433212077017 1105 22-23 0.3626854536731017 1105 24-25 0.32121508096901863 1105 26-27 0.33222599528364327 1105 28-29 0.4840821901554122 1105 30-31 0.4337329971533421 1105 32-33 0.3844883902565428 1105 34-35 0.35028062087590683 1105 36-37 0.3840592039477073 1105 38-39 0.4270339203636979 1105 40-41 0.3902589454318317 1105 42-43 0.3792888297305339 1105 44-45 0.36865161146337755 1105 46-47 0.37967755535532177 1105 48-49 0.20331966235081467 1105 50-51 0.3468402622479658 1105 52-53 0.3135171369437515 1105 54-55 0.45349339180030057 1105 56-57 0.3321333565144684 1105 58-59 0.3143272438436071 1105 60-61 0.325242605247972 1105 62-63 0.3552106236375536 1105 64-65 0.3137828561940026 1105 66-67 0.8515487731444544 1105 68-69 2.122185111265008 1105 70-71 2.3818168553693404 1105 72-73 1.3865445158276195 1105 74-75 1.020836995522199 1105 76-77 0.6864039176631742 1105 78-79 0.5458505656113886 1105 80-81 0.6405002835888105 1105 82-83 0.6266963253693412 1105 84-85 0.47056326981498486 1105 86-87 0.5227805866975679 1105 88-89 0.6513550477001182 1105 90-91 0.7202922893912636 1105 92-93 0.637232468986646 1105 94-95 0.8483964405175755 1105 96-97 0.4445872453566544 1105 98-99 0.7676897086194323 1105 100-101 0.6298873360765214 1106 1 0.3705161567372137 1106 2 0.30510322329518047 1106 3 0.30971582513388896 1106 4 0.21399506484229391 1106 5 0.3072522051122917 1106 6 0.32280619960410206 1106 7 0.40005284701359045 1106 8 0.35570328358333114 1106 9 0.4576955157212623 1106 10-11 0.39508335926546323 1106 12-13 0.4539779370163828 1106 14-15 0.5973027458373537 1106 16-17 0.5414916191550105 1106 18-19 0.5212646113548871 1106 20-21 0.5058944471014115 1106 22-23 0.49962893942661424 1106 24-25 0.6553267831215166 1106 26-27 0.7593239716803879 1106 28-29 0.5781005814538673 1106 30-31 0.6965655746905526 1106 32-33 0.8129956034003953 1106 34-35 0.7826536027601492 1106 36-37 0.8146469969451928 1106 38-39 0.7969848364259562 1106 40-41 0.8271550285242668 1106 42-43 0.7546139933047371 1106 44-45 0.7355096790226767 1106 46-47 0.7455747266073587 1106 48-49 0.7633984488426862 1106 50-51 0.9125723414734566 1106 52-53 0.8760839793660935 1106 54-55 0.9799910123268276 1106 56-57 0.8327159655756162 1106 58-59 0.984145525859077 1106 60-61 1.0770289616879651 1106 62-63 1.0853540458761728 1106 64-65 1.0856756467863278 1106 66-67 1.673850674388131 1106 68-69 3.022042903281754 1106 70-71 3.3919652280336514 1106 72-73 2.2595392431572456 1106 74-75 1.7838972459390128 1106 76-77 1.2603964359820843 1106 78-79 1.2710108706696985 1106 80-81 1.2160923498311114 1106 82-83 1.1172356415567677 1106 84-85 1.1778086381313244 1106 86-87 1.223553039913707 1106 88-89 1.4945526490787628 1106 90-91 1.40489646549565 1106 92-93 1.2903232158937925 1106 94-95 1.6419099817999232 1106 96-97 1.2830072189232489 1106 98-99 1.48078451861182 1106 100-101 1.9753114614624003 1107 1 0.11324771089734043 1107 2 0.11354423283509263 1107 3 0.07194260933869501 1107 4 0.04665778673578558 1107 5 0.03229990466548571 1107 6 0.13434828389343778 1107 7 0.044868690510753595 1107 8 0.09504877325849037 1107 9 0.17300043058661174 1107 10-11 0.1414835524788387 1107 12-13 0.02674692626889197 1107 14-15 0.1923456150560412 1107 16-17 0.11837484581845104 1107 18-19 0.0686743444789002 1107 20-21 0.2383482570277451 1107 22-23 0.3080953082226543 1107 24-25 0.3543800375593875 1107 26-27 0.2664124875601104 1107 28-29 0.13621433583136167 1107 30-31 0.2469701152049879 1107 32-33 0.24639615888886368 1107 34-35 0.1647692892714403 1107 36-37 0.09472669746445206 1107 38-39 0.2187285871806992 1107 40-41 -0.050415313221897406 1107 42-43 -0.004074570878330519 1107 44-45 0.09773042530935783 1107 46-47 0.12472096498436969 1107 48-49 0.15385974578750705 1107 50-51 0.15500803764541615 1107 52-53 0.12413614697869946 1107 54-55 0.2141450999973955 1107 56-57 0.10190446937950526 1107 58-59 0.14040684695556394 1107 60-61 0.13283985409226062 1107 62-63 0.3157247740490945 1107 64-65 0.21404782405960532 1107 66-67 0.76646026260212 1107 68-69 2.2247490981857503 1107 70-71 2.4312068655170442 1107 72-73 1.5122599290647365 1107 74-75 0.9210207817438665 1107 76-77 0.6774378561004291 1107 78-79 0.6794687863803617 1107 80-81 0.5937351465947849 1107 82-83 0.7163118400835167 1107 84-85 0.6232862374550798 1107 86-87 0.5670719385974401 1107 88-89 0.6160500527739714 1107 90-91 0.6990215711592178 1107 92-93 0.7251330214728284 1107 94-95 1.261958285144928 1107 96-97 0.9677916652461569 1107 98-99 1.0105840597300393 1107 100-101 1.1092288627424374 1108 1 -0.23809760910034683 1108 2 -0.24281236079385948 1108 3 -0.20270039233789916 1108 4 -0.13382784661103386 1108 5 -0.06204824819339194 1108 6 -0.03470184040557456 1108 7 -0.08851513327371663 1108 8 0.01221706833856473 1108 9 -0.15759331399337384 1108 10-11 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-0.8060234724246129 2316 54-55 -0.7157015053113938 2316 56-57 -0.7436759850244812 2316 58-59 -0.8123876041582179 2316 60-61 -0.6256933369619375 2316 62-63 -0.8105168337051154 2316 64-65 -0.9249833439631594 2316 66-67 -0.24002233043509946 2316 68-69 0.9874893053797358 2316 70-71 1.360400320676888 2316 72-73 0.36947926124205566 2316 74-75 -0.057438157387341704 2316 76-77 -0.49058579780557565 2316 78-79 -0.5407966531287727 2316 80-81 -0.7206955145585638 2316 82-83 -0.5722280203050865 2316 84-85 -0.7101871519006657 2316 86-87 -0.7202954528035654 2316 88-89 -0.757384654442248 2316 90-91 -0.8870350737209556 2316 92-93 -0.9474720205439766 2316 94-95 -0.5863730473880864 2316 96-97 -0.5866123185695891 2316 98-99 -1.0288437113784035 2316 100-101 -1.2779224422239928 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 11.0 4 39.0 5 102.0 6 192.0 7 506.0 8 1137.0 9 1995.0 10 3092.0 11 4907.0 12 7011.0 13 8927.0 14 10839.0 15 13016.0 16 15942.0 17 18698.0 18 18235.0 19 17057.0 20 17511.0 21 18284.0 22 20401.0 23 23210.0 24 28478.0 25 35087.0 26 43096.0 27 53383.0 28 66558.0 29 80863.0 30 95590.0 31 113095.0 32 136931.0 33 165290.0 34 203654.0 35 255416.0 36 322482.0 37 360380.0 38 218284.0 39 19576.0 40 5.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.69925616323972 19.64732891191727 14.751963394710394 23.901451530132622 2 15.674551891236597 20.67570249146638 39.641888185003175 24.007857432293847 3 19.406414580349804 24.21051447015026 30.098479379681603 26.28459156981833 4 13.22094424836743 17.185085217089764 37.165004226260834 32.42896630828197 5 13.597723320790703 37.50339684672594 34.90345453060163 13.995425301881728 6 32.25191348752377 36.888921861688736 17.223638385451657 13.635526265335827 7 28.364045273506594 32.24653688350358 21.016644965748117 18.37277287724171 8 25.78177489617736 36.334673177497955 19.55091602328028 18.33263590304441 9 25.73663642986824 16.675766603703437 19.949534944591804 37.63806202183652 10-11 24.821780164415717 26.543418786604672 27.93639685839609 20.698404190583524 12-13 25.54474584917834 23.612648606834373 28.104488672453947 22.73811687153334 14-15 22.35387724004328 25.4083509913791 25.86794643735381 26.36982533122382 16-17 22.215660069758414 28.541952472835085 26.620209683694757 22.622177773711744 18-19 22.228756579046085 27.510749040130307 28.250448883917034 22.010045496906578 20-21 22.896559973725495 26.66741411187671 28.329576657036014 22.106449257361778 22-23 22.503025902727646 26.56309132224447 28.262035673976072 22.671847101051814 24-25 22.50013181029404 26.78412266338902 28.33669163039018 22.37905389592676 26-27 22.40087359814277 26.933205794673178 28.161203031335543 22.504717575848513 28-29 22.276236089061683 27.249663545037034 28.150600117285133 22.32350024861615 30-31 22.555788820854332 26.9057351170418 28.07158924523324 22.466886816870623 32-33 22.3950230688644 26.887361129896075 28.06642715921924 22.65118864202028 34-35 22.7270036970811 27.000862791192027 28.012558405128395 22.25957510659848 36-37 22.717809387397995 26.712787710660297 28.123016894546037 22.446386007395667 38-39 22.382015721850284 27.02351811079138 28.107568578410564 22.48689758894777 40-41 22.60324739927056 26.886286368854538 28.14280579419175 22.36766043768315 42-43 22.4607374405914 26.851581037480354 27.97864941218292 22.709032109745326 44-45 22.502069336110246 26.94584347141145 27.969559898936296 22.582527293542 46-47 22.683672615870343 26.99456643152283 27.70827896948887 22.613481983117953 48-49 22.459891486480032 27.03388799924573 27.8075959463978 22.698624567876436 50-51 22.34730876662433 27.149082946277957 27.772557243763853 22.731051043333864 52-53 22.539017221092095 27.048943739371595 27.645510866260096 22.766528173276214 54-55 22.440754987991784 27.050191357796855 27.659370818316546 22.849682835894818 56-57 22.38138401197782 27.13248608570679 27.631145001757396 22.85498490055799 58-59 22.418298719174697 26.858230488527763 27.648272849221446 23.0751979430761 60-61 22.303240994025717 26.988921575676862 27.860335364360424 22.847502065936997 62-63 22.208224626196454 26.98202312708518 27.85632756291989 22.953424683798474 64-65 22.09698760994297 27.084709259541384 27.65995669701016 23.158346433505486 66-67 22.0313036508402 27.108686100381668 27.67489456386857 23.18511568490956 68-69 21.862643816246727 26.847645821281496 28.110524716344088 23.179185646127685 70-71 21.859491378417427 26.750645271652303 27.917899056182314 23.471964293747956 72-73 21.986151596650377 26.8626996139209 27.741258725197742 23.409890064230986 74-75 22.16951210284053 27.194655434317884 27.117567145296416 23.518265317545172 76-77 22.103487662087094 27.0058189894642 27.250498813708322 23.640194534740385 78-79 22.250104881266903 27.082541817562657 27.11828376942335 23.549069531747087 80-81 22.201692577296857 27.096366537660565 27.030658243723376 23.6712826413192 82-83 22.188457512419856 27.136830641314376 27.05530630290251 23.619405543363257 84-85 22.133503273593746 27.140891965467063 26.950501078555206 23.77510368238399 86-87 22.086851525400274 27.122309399728373 26.987746823962684 23.803092250908666 88-89 22.088073125301396 27.104913601127674 26.7938201434681 24.013193130102835 90-91 22.036009830524936 27.11903225221594 26.892108259145576 23.952849658113546 92-93 22.150717039752134 26.989008231686796 26.887641299439043 23.972633429122027 94-95 21.804571025974894 26.887152626782974 27.153139861147697 24.155136486094435 96-97 21.991380465709973 26.7188471492736 27.106678252756993 24.18309413225943 98-99 21.86423677898144 26.882925336648334 26.839195028835324 24.413642855534906 100-101 21.766306415021983 26.748095835065815 27.018268937179492 24.467328812732713 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 217.0 1 145.0 2 88.0 3 121.5 4 127.5 5 129.5 6 199.0 7 395.0 8 651.0 9 841.5 10 876.0 11 820.5 12 873.0 13 991.0 14 1065.5 15 1184.5 16 1344.0 17 1540.5 18 1779.5 19 2089.5 20 2505.5 21 3044.5 22 3760.5 23 4798.0 24 6117.0 25 7742.0 26 10307.5 27 13733.0 28 17075.0 29 20580.5 30 24832.5 31 30002.5 32 36024.5 33 41139.0 34 45530.0 35 50449.5 36 56657.5 37 62985.0 38 67511.5 39 70682.5 40 74687.5 41 78208.5 42 80549.5 43 83352.0 44 86451.0 45 90391.0 46 93332.0 47 95976.0 48 98812.0 49 98435.5 50 95202.0 51 92250.5 52 89931.5 53 87750.0 54 84218.5 55 77756.5 56 70062.5 57 62405.5 58 55696.5 59 48442.0 60 39647.0 61 31769.0 62 25445.5 63 19231.5 64 13532.0 65 9755.0 66 7099.5 67 5211.5 68 3835.0 69 2763.0 70 2025.5 71 1375.5 72 912.0 73 677.0 74 465.0 75 305.0 76 211.5 77 112.5 78 60.5 79 38.0 80 18.0 81 11.5 82 6.0 83 6.0 84 6.0 85 2.5 86 1.0 87 0.5 88 0.0 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02104794597054788 2 0.022339998099432996 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.4587685326122293E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.0419775232944495E-4 26-27 4.7930966071544674E-4 28-29 1.2503730279533393E-4 30-31 5.418283121131137E-4 32-33 0.0022715110007818996 34-35 0.00391783548758713 36-37 0.10315577480615051 38-39 0.09592445079448703 40-41 0.10438530828363794 42-43 0.10565652086205718 44-45 0.09919626021763159 46-47 0.09338202563764857 48-49 0.09400721215162523 50-51 0.09304859283019434 52-53 0.07573092639304059 54-55 0.06462344599472175 56-57 0.035468914892943064 58-59 0.015087834537303628 60-61 0.010482293884342162 62-63 0.0026049438082361236 64-65 0.005939271882778362 66-67 0.16156903476203735 68-69 0.4292113813954496 70-71 0.4078925212688452 72-73 0.19036929350589593 74-75 0.030654978735322705 76-77 0.035156321635954724 78-79 0.005939271882778362 80-81 0.001813040890532342 82-83 2.0839550465888988E-5 84-85 3.9595145885189085E-4 86-87 0.0011878543765556726 88-89 2.083955046588899E-4 90-91 9.377797709650046E-4 92-93 0.00331348852407635 94-95 0.08287889220284052 96-97 0.01794285295113042 98-99 0.02042275945657121 100-101 0.011107480398318832 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2399284.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.17614661722552 #Duplication Level Percentage of deduplicated Percentage of total 1 73.83954310368888 42.218605426454594 2 14.591514647770579 16.68573161736649 3 5.022908478768909 8.61571654880989 4 2.3667752032209095 5.4129234411748985 5 1.23963590369228 3.543880209074332 6 0.7371701929464425 2.528913062025253 7 0.4759303861953606 1.9048305878489091 8 0.3185774383107069 1.4572024257434475 9 0.23703451676724369 1.2197448255624415 >10 1.0876633946012346 10.827112030569998 >50 0.05941530423267527 2.2878112920688083 >100 0.022153203525501045 2.259551031785109 >500 0.0013133944793747878 0.49370682286867296 >1k 3.6483179982394094E-4 0.544270678647275 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4157 0.17326002257340106 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3693 0.1539209197410561 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2411 0.10048831234651671 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.167910093177798E-5 2 0.0 0.0 0.0 0.0 4.167910093177798E-5 3 0.0 0.0 0.0 0.0 4.167910093177798E-5 4 0.0 0.0 0.0 0.0 4.167910093177798E-5 5 0.0 0.0 0.0 0.0 4.167910093177798E-5 6 0.0 0.0 0.0 0.0 4.167910093177798E-5 7 0.0 0.0 0.0 0.0 4.167910093177798E-5 8 0.0 0.0 0.0 0.0 4.167910093177798E-5 9 0.0 0.0 0.0 0.0 8.335820186355597E-5 10-11 0.0 0.0 0.0 2.083955046588899E-5 8.335820186355597E-5 12-13 4.167910093177798E-5 0.0 0.0 8.335820186355597E-5 1.6671640372711193E-4 14-15 4.167910093177798E-5 0.0 0.0 8.335820186355597E-5 1.6671640372711193E-4 16-17 8.335820186355597E-5 0.0 0.0 1.0419775232944495E-4 1.6671640372711193E-4 18-19 8.335820186355597E-5 0.0 0.0 1.2503730279533393E-4 2.083955046588899E-4 20-21 8.335820186355597E-5 0.0 0.0 1.875559541930009E-4 2.5007460559066787E-4 22-23 1.2503730279533393E-4 0.0 0.0 3.3343280745422386E-4 2.7091415605655687E-4 24-25 1.2503730279533393E-4 0.0 0.0 6.043469635107807E-4 2.9175370652244586E-4 26-27 1.2503730279533393E-4 0.0 0.0 0.0012086939270215613 3.3343280745422386E-4 28-29 2.083955046588899E-4 0.0 0.0 0.004563861552029689 3.3343280745422386E-4 30-31 2.5007460559066787E-4 0.0 0.0 0.018672237217436535 3.542723579201128E-4 32-33 2.5007460559066787E-4 0.0 0.0 0.05547488334019649 3.751119083860018E-4 34-35 2.9175370652244586E-4 0.0 0.0 0.1120542628550851 4.167910093177798E-4 36-37 3.751119083860018E-4 0.0 0.0 0.20281050513403165 4.167910093177798E-4 38-39 3.751119083860018E-4 0.0 0.0 0.34083084786961443 4.376305597836688E-4 40-41 3.751119083860018E-4 0.0 0.0 0.4900211896549137 5.001492111813357E-4 42-43 4.167910093177798E-4 0.0 0.0 0.6432335646801296 5.001492111813357E-4 44-45 5.001492111813357E-4 0.0 0.0 0.8283929705695532 5.001492111813357E-4 46-47 5.418283121131137E-4 0.0 0.0 1.0489170935995906 5.001492111813357E-4 48-49 5.418283121131137E-4 0.0 0.0 1.2787356561374144 5.001492111813357E-4 50-51 5.418283121131137E-4 0.0 0.0 1.524913265790961 5.001492111813357E-4 52-53 5.418283121131137E-4 0.0 0.0 1.7643597006440255 6.460260644425588E-4 54-55 5.418283121131137E-4 0.0 0.0 2.021436395191232 7.085447158402257E-4 56-57 5.835074130448917E-4 0.0 0.0 2.320546463028137 7.085447158402257E-4 58-59 5.835074130448917E-4 0.0 0.0 2.6303680598045083 7.085447158402257E-4 60-61 5.835074130448917E-4 0.0 0.0 2.935500757726055 7.085447158402257E-4 62-63 5.835074130448917E-4 0.0 0.0 3.224482804036538 7.085447158402257E-4 64-65 5.835074130448917E-4 0.0 0.0 3.530365725774856 7.502238167720036E-4 66-67 6.668656149084477E-4 0.0 0.0 3.8595264253835726 7.502238167720036E-4 68-69 6.668656149084477E-4 0.0 0.0 4.23511764343029 7.502238167720036E-4 70-71 6.668656149084477E-4 0.0 0.0 4.657243577667337 7.502238167720036E-4 72-73 6.668656149084477E-4 0.0 0.0 5.086413279961855 7.919029177037816E-4 74-75 6.668656149084477E-4 0.0 0.0 5.520938746726106 7.919029177037816E-4 76-77 6.668656149084477E-4 0.0 0.0 5.987890554015282 7.919029177037816E-4 78-79 6.668656149084477E-4 0.0 0.0 6.514089203278978 8.752611195673376E-4 80-81 8.335820186355596E-4 0.0 0.0 7.056876134713523 8.752611195673376E-4 82-83 8.335820186355596E-4 0.0 0.0 7.60512302837013 8.752611195673376E-4 84-85 8.335820186355596E-4 0.0 0.0 8.139053150856672 8.752611195673376E-4 86-87 8.335820186355596E-4 0.0 0.0 8.684695100705044 8.961006700332265E-4 88-89 8.335820186355596E-4 0.0 0.0 9.279497550102448 9.169402204991156E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5425 0.0 32.891945 1 GTATCAA 7740 0.0 26.119814 1 ATCAACG 8945 0.0 22.168404 3 TCAACGC 8985 0.0 22.069714 4 CAACGCA 9030 0.0 21.95973 5 TATCAAC 9300 0.0 21.480593 2 AACGCAG 9280 0.0 21.368143 6 TACATGG 6730 0.0 20.72905 2 GTACATG 7170 0.0 20.71699 1 ACATGGG 6805 0.0 19.937761 3 ACGCAGA 10330 0.0 19.104317 7 GAGTACT 5330 0.0 18.957928 12-13 CGCAGAG 10355 0.0 18.920753 8 AGAGTAC 8270 0.0 18.75775 10-11 GTACTTT 5770 0.0 17.553373 14-15 GTGTAGC 1685 0.0 17.46197 1 AGTACTT 5845 0.0 17.368717 12-13 GTAAGGT 955 0.0 16.889393 4 TAAGGTG 1205 0.0 16.534868 5 GCAGAGT 11650 0.0 16.491783 9 >>END_MODULE