FastQCFastQC Report
Fri 27 May 2016
SRR939791_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939791_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2449043
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44560.1819486223802522No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32760.1337665365614242No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30250.12351763525589383No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG87550.025.630992
GTACATG86850.025.509821
ACATGGG91200.023.7218273
GTATCAA114050.023.0448991
GGACCGA3500.023.0435876
CATGGGG40850.021.4861094
ATCAACG131550.020.5571123
CAACGCA132250.020.4483055
TCAACGC132950.020.412014
GGTATCA85800.020.4032551
TATCAAC133300.020.2864042
AACGCAG135400.020.0072176
GAGTACT52300.019.63786912-13
ACCGAGT4750.018.9762958
GTATAGG7950.018.4992831
ACGCAGA147900.018.0275787
AGTACTT56250.018.00584612-13
CGCAGAG147650.017.960978
GTACTTT58050.017.85777314-15
AGAGTAC110100.017.75221310-11
Produced by FastQC (version 0.11.5)