FastQCFastQC Report
Fri 27 May 2016
SRR939791_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939791_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2449043
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47710.19481078935731222No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41020.1674939966346038No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34830.14221881771777792No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27230.11118628786836326No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG93500.024.8199881
TACATGG95100.024.0463712
ACATGGG95800.022.787413
GTATCAA121600.022.7306611
GGTATCA91050.021.965141
GAGTACT53400.020.86886412-13
TCAACGC138800.019.498534
CAACGCA139750.019.3997215
ATCAACG139500.019.3668883
AACGCAG140500.019.3174886
CATGGGA54750.019.2043764
GTACTTT59100.019.09551814-15
TATCAAC143100.019.0118562
AGAGTAC113100.018.16470710-11
CATGGGG40850.018.0057834
GTCGCGA1102.433544E-517.64331870-71
AGTACTT57800.017.56824512-13
ACGCAGA153850.017.518757
CGCAGAG153350.017.5144148
GTATAGG7950.017.2059631
Produced by FastQC (version 0.11.5)